Protein detail

ESYT2

Extended synaptotagmin-2 (E-Syt2) (Chr2Syt)

Entry name ESYT2	UniProt ID A0FGR8	EVMP confidence score 0.72
Supporting publications (n) 19	Transmembrane count 2	Protein classification Plasma proteinsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Extended synaptotagmin-2 (E-Syt2) (Chr2Syt)

Protein Class (3)

Plasma proteinsPredicted membrane proteinsTransporters

Protein Function

Transporters

Transmembrane

104..124; Helical; 128..148; Helical

Transmembrane Count

Ensembl

ENSG00000117868

Entrez Gene Symbol

ESYT2

Gene Synonym (3)

CHR2SYTFAM62BKIAA1228

Gene Description

Extended synaptotagmin 2

Chromosome

Position

158730995-158830253

Supporting publications (n)

EVMP confidence score

0.72

Fluorescence & Localization5

Tissue Specificheart muscleCell SpecificAdipocytesBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes

Function & Pathway6

Protein Function

Transporters

Cellular Component (7)

Molecular Function (8)

Biological Process (3)

Reactome (2)

Mediation Categories

Fusion and delivery mediation

Relations & Evidence19

Ligand-Receptor Signaling (15)

15 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane	transmembrane	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	No	No

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Protein Complex Composition (3)

3 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
ESYT1-ESYT2 complex	ESYT1 ESYT2	A0FGR8 Q9BSJ8	0:0	CORUM	CORUM:7313	23791178
	ESYT2	A0FGR8	2	PDB	PDB:4p42PDB:4npj
	COX15 ESYT2	A0FGR8 Q7KZN9	0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	2	27821849 38716512

Sequence, Structure & Domains15

Sequences

Length

921

Mass

102,357

Sequence

MTANRDAALSSHRHPGCAQRPRTPTFASSSQRRSAFGFDDGNFPGLGERSHAPGSRLGARRRAKTARGLRGHRQRGAGAGLSRPGSARAPSPPRPGGPENPGGVLSVELPGLLAQLARSFALLLPVYALGYLGLSFSWVLLALALLAWCRRSRGLKALRLCRALALLEDEERVVRLGVRACDLPAWVHFPDTERAEWLNKTVKHMWPFICQFIEKLFRETIEPAVRGANTHLSTFSFTKVDVGQQPLRINGVKVYTENVDKRQIILDLQISFVGNCEIDLEIKRYFCRAGVKSIQIHGTMRVILEPLIGDMPLVGALSIFFLRKPLLEINWTGLTNLLDVPGLNGLSDTIILDIISNYLVLPNRITVPLVSEVQIAQLRFPVPKGVLRIHFIEAQDLQGKDTYLKGLVKGKSDPYGIIRVGNQIFQSRVIKENLSPKWNEVYEALVYEHPGQELEIELFDEDPDKDDFLGSLMIDLIEVEKERLLDEWFTLDEVPKGKLHLRLEWLTLMPNASNLDKVLTDIKADKDQANDGLSSALLILYLDSARNLPSGKKISSNPNPVVQMSVGHKAQESKIRYKTNEPVWEENFTFFIHNPKRQDLEVEVRDEQHQCSLGNLKVPLSQLLTSEDMTVSQRFQLSNSGPNSTIKMKIALRVLHLEKRERPPDHQHSAQVKRPSVSKEGRKTSIKSHMSGSPGPGGSNTAPSTPVIGGSDKPGMEEKAQPPEAGPQGLHDLGRSSSSLLASPGHISVKEPTPSIASDISLPIATQELRQRLRQLENGTTLGQSPLGQIQLTIRHSSQRNKLIVVVHACRNLIAFSEDGSDPYVRMYLLPDKRRSGRRKTHVSKKTLNPVFDQSFDFSVSLPEVQRRTLDVAVKNSGGFLSKDKGLLGKVLVALASEELAKGWTQWYDLTEDGTRPQAMT

Alternative Products

Event=Alternative splicing; Named isoforms=5; Name=1; IsoId=A0FGR8-1; Sequence=Displayed; Name=2; IsoId=A0FGR8-2; Sequence=VSP_023239; Name=4; IsoId=A0FGR8-4; Sequence=VSP_023238, VSP_023241, VSP_023242; Name=5; IsoId=A0FGR8-5; Sequence=VSP_023236, VSP_023240; Name=6; IsoId=A0FGR8-6; Sequence=VSP_038324

Alternative Sequence

1..593; Missing (in isoform 5); 1..204; Missing (in isoform 4); 1..97; MTANRDAALSSHRHPGCAQRPRTPTFASSSQRRSAFGFDDGNFPGLGERSHAPGSRLGARRRAKTARGLRGHRQRGAGAGLSRPGSARAPSPPRPGG -> MTPPSRAEAGVRRSRVPSEGRWRGAEPPGISASTQPASAGRAARHCGAMSGARGEGPEAGAGGAGGRAA (in isoform 2); 550; S -> SNPLEFNPDVLKKTAVQRALKS (in isoform 6); 594..603; NPKRQDLEVE -> MPVLPPCVLQ (in isoform 5); 706..731; PVIGGSDKPGMEEKAQPPEAGPQGLH -> SQSRSRPPASPRTSRCPSPPRSCGKG (in isoform 4); 732..921; Missing (in isoform 4)

3D Structural Models

Turn

283..286; 341..345; 405..407; 595..597; 607..609; 798..801; 899..902

Helix

196..219; 221..226; 230..232; 334..337; 348..358; 372..379; 476..482; 515..524; 621..624; 627..629; 862..867

Beta Strand

235..241; 248..255; 264..282; 287..304; 316..323; 326..333; 365..370; 384..396; 414..420; 423..426; 437..446; 453..460; 463..465; 467..475; 483..490; 494..496; 498..512; 535..547; 552..554; 560..566; 569..572; 583..593; 599..606; 612..619; 630..636; 638..640; 645..656; 789..797; 802..811; 823..831; 835..837; 851..858; 869..876; 889..894

3D Structure

NMR spectroscopy (1); X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias

58..75; Basic residues

Domain (CC)

Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic.; DOMAIN: The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium (PubMed:24373768). The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate and is required for location at the cell membrane (PubMed:23791178).; DOMAIN: The SMP-LTD domain is a barrel-like domain that binds glycerophospholipids in its interior; can bind two lipid molecules simultaneously. Binds a variety of lipids, including phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol (PubMed:24847877).

Domain (FT)

191..370; SMP-LTD; 369..489; C2 1; 514..639; C2 2; 786..908; C2 3

Region

1..103; Disordered; 660..754; Disordered; 833..840; Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membrane

Protein Families

Extended synaptotagmin family

Sequence Similarities

Belongs to the extended synaptotagmin family.

Clinical Relevance5

Drugs

METHYLPHENIDATE HYDROCHLORIDE

Antibody (2)

HPA002132 HPA070823

Interaction Protein (4)

ENSG00000101558ENSG00000124164ENSG00000139641ENSG00000170027

Interaction Count

Interaction Dataset (2)

biogrid_opencellintact_biogrid_opencell

Supporting Publications18

PMID	Title	Abstract
36398564	Differential ultracentrifugation enables deep plasma proteomics through enrichment of extracellular vesicles.	No abstract available
37786918	Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples.	No abstract available
38168906	Defining the relationship between cellular and extracellular vesicle (EV) content in breast cancer via an integrative multi-omic analysis.	No abstract available
38321535	Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles.	No abstract available
39569406	Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides.	No abstract available
39766158	A Proteomic Examination of Plasma Extracellular Vesicles Across Colorectal Cancer Stages Uncovers Biological Insights That Potentially Improve Prognosis.	No abstract available
40748658	Exosomes released from senescent cells and circulatory exosomes isolated from human plasma reveal aging-associated proteomic and lipid signatures.	No abstract available
41216884	Extracellular Vesicles From Multiple Sclerosis White Matter Exhibit Synaptic, Mitochondrial, Complement and Ageing-Related Pathway Dysregulation.	No abstract available

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