Protein detail

SPD2B

SH3 and PX domain-containing protein 2B (Adapter protein HOFI) (Factor for adipocyte differentiation 49) (Tyrosine kinase substrate with four SH3 domains)

Protein symbol SPD2B	UniProt ID A1X283	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification Disease related genesHuman disease related genesPredicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

SH3 and PX domain-containing protein 2B (Adapter protein HOFI) (Factor for adipocyte differentiation 49) (Tyrosine kinase substrate with four SH3 domains)

Protein Class

Disease related genesHuman disease related genesPredicted intracellular proteins

Protein Function

Disease related genes
Human disease related genes:Congenital malformations:Other congenital malformations
Predicted intracellular proteins

Ensembl

ENSG00000174705

Entrez Gene Symbol

SH3PXD2B

Gene Synonym

FLJ20831KIAA1295

Gene Description

SH3 and PX domains 2B

Chromosome

Position

172325000-172454525

Frequency

EVMP Score

0.38

Fluorescence & Localization

Cell SpecificRetinal horizontal cellsSecretome LocationIntracellular and membraneSecretome FunctionDevelopmental protein

Function & Pathway

Protein Function

Disease related genes
Human disease related genes:Congenital malformations:Other congenital malformations
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

Canonical Pathways

M17200 Sa b cell receptor complexes
M8626 Sig bcr signaling pathway
M141 Pid pi3kci pathway

Mediation Categories

Immune mediationMetabolism mediation

Relations & Evidence

Enzyme-Mediated Modification

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
SH3PXD2B	SRC	P12931	Y	508	phosphorylation	PhosphoSite_MIMPMIMPSIGNORProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20943948 SIGNOR:20943948

Ligand-Receptor Signaling

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
SRC	P12931	SPD2B	A1X283	Yes	Yes	No	PhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:20943948 SIGNOR:20943948 ProtMapper:20943948
SPD2B	A1X283	NOXA1	Q86UR1	Yes	Yes	No	SIGNOR	SIGNOR:20943948

Protein Complex Composition

6 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
AHSG DIS3 PPP5C SH3PXD2B STXBP3	A1X283 O00186 P02765 P53041 Q9Y2L1	0:0:0:0:0	Havugimana2012	Havugimana2012:C_431
NEBL SH3PXD2A SH3PXD2B WHR1 ZNF764	A1X283 O76041 P49842 Q5TCZ1 Q96H86	0:0:0:0:0	hu.MAP2
NEBL SH3PXD2B ZNF764	A1X283 O76041 Q96H86	0:0:0	hu.MAP2
ARPP19 RABIF SH3PXD2B TRIM25	A1X283 P47224 P56211 Q14258	0:0:0:0	hu.MAP
CBLB SH3PXD2B	A1X283 Q13191	0:0	hu.MAP
ENPP2 PSPC1 SH3PXD2B	A1X283 Q13822 Q8WXF1	0:0:0	hu.MAP

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	30950185

Sequence, Structure & Domains

Sequences

Length

911

Mass

101,579

Sequence

MPPRRSIVEVKVLDVQKRRVPNKHYVYIIRVTWSSGSTEAIYRRYSKFFDLQMQMLDKFPMEGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLIPIDEYCKALIQLPPYISQCDEVLQFFETRPEDLNPPKEEHIGKKKSGGDQTSVDPMVLEQYVVVANYQKQESSEISLSVGQVVDIIEKNESGWWFVSTAEEQGWVPATCLEGQDGVQDEFSLQPEEEEKYTVIYPYTARDQDEMNLERGAVVEVIQKNLEGWWKIRYQGKEGWAPASYLKKNSGEPLPPKPGPGSPSHPGALDLDGVSRQQNAVGREKELLSSQRDGRFEGRPVPDGDAKQRSPKMRQRPPPRRDMTIPRGLNLPKPPIPPQVEEEYYTIAEFQTTIPDGISFQAGLKVEVIEKNLSGWWYIQIEDKEGWAPATFIDKYKKTSNASRPNFLAPLPHEVTQLRLGEAAALENNTGSEATGPSRPLPDAPHGVMDSGLPWSKDWKGSKDVLRKASSDMSASAGYEEISDPDMEEKPSLPPRKESIIKSEGELLERERERQRTEQLRGPTPKPPGVILPMMPAKHIPPARDSRRPEPKPDKSRLFQLKNDMGLECGHKVLAKEVKKPNLRPISKSKTDLPEEKPDATPQNPFLKSRPQVRPKPAPSPKTEPPQGEDQVDICNLRSKLRPAKSQDKSLLDGEGPQAVGGQDVAFSRSFLPGEGPGRAQDRTGKQDGLSPKEISCRAPPRPAKTTDPVSKSVPVPLQEAPQQRPVVPPRRPPPPKKTSSSSRPLPEVRGPQCEGHESRAAPTPGRALLVPPKAKPFLSNSLGGQDDTRGKGSLGPWGTGKIGENREKAAAASVPNADGLKDSLYVAVADFEGDKDTSSFQEGTVFEVREKNSSGWWFCQVLSGAPSWEGWIPSNYLRKKP

3D Structural Models

Domain & Motif Annotations

Compositional Bias

282..292; Pro residues; 311..337; Basic and acidic residues; 338..347; Basic residues; 486..499; Basic and acidic residues; 517..548; Basic and acidic residues; 571..586; Basic and acidic residues; 598..609; Basic and acidic residues; 618..628; Basic and acidic residues; 643..653; Pro residues; 756..766; Pro residues; 822..831; Gly residues

Domain (CC)

The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-phosphate (PtdIns(5)P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain (By similarity).

Domain (FT)

5..129; PX; 152..211; SH3 1; 221..280; SH3 2; 368..427; SH3 3; 850..911; SH3 4

Region

275..366; Disordered; 458..834; Disordered

Protein Families

SH3PXD2 family

Sequence Similarities

Belongs to the SH3PXD2 family.

Clinical Relevance

Disease Involvement

Disease variant

Antibody

HPA036471 HPA058141