Protein detail

DFFA

DNA fragmentation factor subunit alpha (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) (ICAD)

Entry name DFFA	UniProt ID O00273	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

DNA fragmentation factor subunit alpha (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) (ICAD)

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000160049

Entrez Gene Symbol

DFFA

Gene Synonym (4)

DFF-45DFF1DFF45ICAD

Gene Description

DNA fragmentation factor subunit alpha

Chromosome

Position

10456522-10472529

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Cell SpecificNeutrophilsSingle-Nuclei Brain Specificoligodendrocyte

Function & Pathway7

Protein Function

Predicted intracellular proteins

Cellular Component (6)

Molecular Function (4)

Biological Process (3)

KEGG

hsa04210 Apoptosis

Reactome (4)

Mediation Categories

Other mediation

Relations & Evidence14

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
DFFA	GZMB	P10144	D	117	cleavage	HPRD	HPRD:11406587
DFFA	GZMB	P10144	D	224	cleavage	HPRD	HPRD:11406587

Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
CASP3	P42574	DFFA	O00273	Yes	Yes	Yes	WangMacrophageSIGNORHPRDCui2007HINTACSNCA1InnateDBSPIKE_LCLit-BM-17SPIKE	Lit-BM-17:18723680 HPRD:11752060 ACSN:9422506 CA1:12655299 HPRD:9108473 HINT:11406587 SIGNOR:9108473 SPIKE_LC:9108473 CA1:9422513 Macrophage:9422506 SPIKE_LC:9422506 Macrophage:9786842 ACSN:9108473 InnateDB:11406587 SPIKE:9108473 SPIKE:9422506 Lit-BM-17:25241761 HINT:18723680 SPIKE_LC:16189514
DFFA	O00273	DFFB	O76075	Yes	No	Yes	WangSIGNORHPRDCui2007HINTHuRIBioGRIDCA1IntActSPIKE_LCSPIKE	BioGRID:15572351 SPIKE_LC:10527860 SPIKE:10932250 IntAct:32296183 SPIKE_LC:17145710 HINT:9108473 IntAct:11371636 HINT:9422506 CA1:12655299 IntAct:9108473 HPRD:9422506 SIGNOR:9108473 HPRD:10409614 HINT:17353931 SPIKE_LC:10932250 HINT:11371636 CA1:9422513 SPIKE_LC:9108473 IntAct:19882353 HPRD:10932250 SPIKE_LC:17353931 HPRD:11371636 HINT:33961781 BioGRID:10527860 SPIKE:10527860 HINT:19882353 IntAct:17353931 HPRD:10527861 IntAct:33961781 HPRD:10527860
	COMPLEX:P42574	DFFA	O00273	Yes	Yes	No	SIGNOR	SIGNOR:9108473

Protein Complex Composition (3)

3 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
DNA fragmentation factor complex	DFFA DFFB	O00273 O76075	1:1	ComplexPortal	intact:EBI-9018105PDB:1ibx	19944011 22727028 14755292
	DFFA DFFB TSPYL4	O00273 O76075 Q9UJ04	0:0:0	hu.MAPhu.MAP2
	DFFA SEPTIN10 SEPTIN5	O00273 Q99719 Q9P0V9	0:0:0	Havugimana2012	Havugimana2012:C_129

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	R SequencingMass spectrometry	15	31508500 38644352 32795414 38731868 25471207 26884841 30071318 30608700 22106071 37686366 38490958 41201090 30760538 37922300 40091455

Sequence, Structure & Domains10

Sequences

Length

331

Mass

36,522

Sequence

MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=DFF45; IsoId=O00273-1; Sequence=Displayed; Name=DFF35; IsoId=O00273-2; Sequence=VSP_001085, VSP_001086

Alternative Sequence

262..268; LVTKEDP -> VGGNQGH (in isoform DFF35); 269..331; Missing (in isoform DFF35)

3D Structural Models

Turn

251..254

Helix

239..246; 257..264; 268..274; 279..297; 298..300

3D Structure

NMR spectroscopy (3)

Domain & Motif Annotations

Domain (FT)

17..96; CIDE-N

Region

305..331; Disordered

Clinical Relevance4

Antibody (4)

CAB002679 HPA018859 HPA019938 HPA025230

Interaction Protein (3)

ENSG00000106211ENSG00000164924ENSG00000169598

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_bioplex

Supporting Publications4

PMID	Title	Abstract
27821849	Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.	No abstract available
31382537	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.	No abstract available
34815502	The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients.	No abstract available
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available