Protein detail

DDX3X

ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)

Entry name DDX3X	UniProt ID O00571	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count	Protein classification Cancer-related genesDisease related genesEnzymesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)

Protein Class (10)

Cancer-related genesDisease related genesEnzymesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function (8)

Human disease related genes:Other diseases:Mental and behavioural disorders
Predicted intracellular proteins
Cancer-related genes:Mutated cancer genes
Potential drug targets
Enzymes
Transporters:Transporter channels and pores
ENZYME proteins:Hydrolases
Disease related genes

Ensembl

ENSG00000215301

Entrez Gene Symbol

DDX3X

Gene Synonym (5)

CAP-RfDBXDDX14DDX3HLP2

Gene Description

DEAD-box helicase 3 X-linked

Chromosome

Position

41333348-41364472

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization1

Cell SpecificEarly spermatids

Function & Pathway8

Protein Function (8)

Human disease related genes:Other diseases:Mental and behavioural disorders
Predicted intracellular proteins
Cancer-related genes:Mutated cancer genes
Potential drug targets
Enzymes
Transporters:Transporter channels and pores
ENZYME proteins:Hydrolases
Disease related genes

Cellular Component (17)

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Molecular Function (23)

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Biological Process (3)

KEGG (3)

Reactome (7)

Canonical Pathways

M177 Pid epha fwdpathway

Mediation Categories (3)

Clinical-translation mediationImmune mediationReceptor-signaling mediation

Relations & Evidence69

Enzyme-Mediated Modification (8)

8 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
DDX3X	CDK1	P06493	T	323	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:16280325 phosphoELM:16280325 SIGNOR:16280325 KEA:16280325
DDX3X	CDK1	P06493	T	204	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:16280325 ProtMapper:16280325 phosphoELM:16280325 SIGNOR:16280325 ProtMapper:17379183
DDX3X	CCNB1	P14635	T	323	phosphorylation	SIGNOR	SIGNOR:16280325
DDX3X	CCNB1	P14635	T	204	phosphorylation	SIGNOR	SIGNOR:16280325
DDX3X	IKBKE	Q14164	S	102	phosphorylation	Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:28402257 ProtMapper:25538732
DDX3X	CDK2	P24941	T	204	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
DDX3X	AURKB	Q96GD4	S	594	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
DDX3X	IGF1R	P08069	Y	104	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (10)

10 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (7)

7 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
TBK1	Q9UHD2	DDX3X	O00571	Yes	Yes	No	WangSPIKE_LCSIGNOR	SIGNOR:18583960 SPIKE_LC:18583960
DDX3X	O00571	SP1	P08047	Yes	Yes	No	InnateDBSIGNOR	InnateDB:16818630 SIGNOR:33627125
DDX3X	O00571	IF4E	P06730	Yes	No	Yes	SIGNORELMHINTIntActInnateDBLMPID	HINT:21883093 HINT:19262567 IntAct:18596238 IntAct:22872150 HINT:17667941 IntAct:21883093 InnateDB:17667941 LMPID:17667941 ELM:17667941 SIGNOR:17667941 HINT:18596238
DDX3X	O00571	PABP1	P11940	Yes	Yes	No	HINTSIGNORIntAct	HINT:21883093 IntAct:18596238 IntAct:22872150 HINT:22872150 IntAct:21883093 SIGNOR:21883093 HINT:18596238
FUS	P35637	DDX3X	O00571	Yes	No	Yes	SIGNOR	SIGNOR:27460707
CDK1	P06493	DDX3X	O00571	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperRLIMS-P_ProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:16280325 KEA:16280325 ProtMapper:16280325 phosphoELM:16280325 PhosphoSite:17379183 SIGNOR:16280325 ProtMapper:17379183
	COMPLEX:P06493_P14635	DDX3X	O00571	Yes	No	Yes	SIGNOR	SIGNOR:16280325

Protein Complex Composition (43)

43 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
CDK12 DDX39B DDX3X EIF4E EIF4G3 ELAVL2 ELAVL4 EWSR1 NCBP1 POLDIP3 SRSF2 THOC2	O00571 O43432 P06730 P26378 Q01130 Q01844 Q09161 Q12926 Q13838 Q8NI27 Q9BY77 Q9NYV4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5740
CDK12 DDX39B DDX3X EIF4G3 EWSR1 NCBP1 POLDIP3 RBM39 SRSF1 SRSF2 THOC2 UBC	O00571 O43432 P0CG48 Q01130 Q01844 Q07955 Q09161 Q13838 Q14498 Q8NI27 Q9BY77 Q9NYV4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5032
DDX3X EIF4G3 NCBP1 NCBP2 POLDIP3 PUF60 RBM39 RNPS1 SF3A2 SRSF1 SRSF2 SRSF9 TRNAU1AP U2AF2	O00571 O43432 P26368 P52298 Q01130 Q07955 Q09161 Q13242 Q14498 Q15287 Q15428 Q9BY77 Q9NX07 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9211
DDX3X EIF4E EIF4G1 EIF4G2 FBL LARP1 NCBP1 PABPN1 POLDIP3 PRMT3 SRSF1 SRSF2 UBC	O00571 O60678 P06730 P0CG48 P22087 P78344 Q01130 Q04637 Q07955 Q09161 Q6PKG0 Q86U42 Q9BY77	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9516
DDX3X FGD2 LUC7L3 NCBP1 PRPF40A PUF60 RNPS1 SF1 SNRNP70 SRPK1 SRPK2 SRSF1 SRSF2 U2AF2	O00571 O75400 O95232 P08621 P26368 P78362 Q01130 Q07955 Q09161 Q15287 Q15637 Q7Z6J4 Q96SB4 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7919
CELF2 CELF3 DDX3X EIF4G2 EWSR1 NCBP1 PCBP1 POLDIP3 PUF60 SRSF2 ZC3H14 ZCCHC7	O00571 O95319 P78344 Q01130 Q01844 Q09161 Q15365 Q5SZQ8 Q6PJT7 Q8N3Z6 Q9BY77 Q9UHX1	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9738
DDX3X ESR1 ESR2 ZNF512B	O00571 P03372 Q92731 Q96KM6	1:1:1:1	CompleatCFinder	Compleat:HC7120
DDX3X EIF4E EIF4G1 EIF4G2 FBL LARP1 NCBP1 PABPN1 POLDIP3 SRSF1 SRSF2 U2AF2	O00571 P06730 P22087 P26368 P78344 Q01130 Q04637 Q07955 Q09161 Q6PKG0 Q86U42 Q9BY77	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3798
DDX3X PPP2R2B RPL11 RPL13A RPL14 RPL17 RPL26 RPL30 RPL7 RPS11 RPS14 RPS15A UBC	O00571 P0CG48 P18124 P18621 P40429 P50914 P61254 P62244 P62263 P62280 P62888 P62913 Q00005	1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9038
DDX1 DDX3X DIS3L2 PUF60 UBC UGP2	O00571 P0CG48 Q16851 Q8IYB7 Q92499 Q9UHX1	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3958

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryAntibody array	6	38113368 40189497 33592500 38014595 36146834 27821849

Sequence, Structure & Domains16

Sequences

Length

662

Mass

73,243

Sequence

MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O00571-1; Sequence=Displayed; Name=2; IsoId=O00571-2; Sequence=VSP_042830

Alternative Sequence

35..51; KGRYIPPHLRNREATKG -> S (in isoform 2)

3D Structural Models

Turn

158..161; 355..357; 411..414; 499..501; 502..504

Helix

144..151; 162..165; 182..184; 189..198; 205..215; 230..245; 249..256; 276..290; 306..313; 324..332; 349..354; 358..365; 387..396; 424..426; 427..437; 451..463; 477..480; 482..488; 522..529; 547..552; 553..562; 569..575; 578..580

Beta Strand

136..138; 168..172; 220..223; 261..263; 268..272; 297..300; 302..304; 318..322; 343..348; 367..369; 376..383; 401..405; 415..421; 444..449; 468..471; 473..475; 491..498; 509..517; 535..537; 539..545

3D Structure

X-ray crystallography (16)

Domain & Motif Annotations

Compositional Bias

21..34; Polar residues; 44..68; Basic and acidic residues; 70..89; Low complexity; 94..130; Basic and acidic residues; 604..622; Low complexity; 623..634; Gly residues

Motif

12..21; Nuclear export signal; 180..208; Q motif; 347..350; DEAD box

Domain (CC)

The C-terminus (residues 536-662) is dispensable for DDX3X trafficking.

Domain (FT)

211..403; Helicase ATP-binding; 414..575; Helicase C-terminal

Region

2..139; Required for TBK1 and IKBKE-dependent IFNB1 activation; 19..144; Disordered; 38..44; Interaction with EIF4E; 81..90; Interaction with VACV protein K7; 88..123; Involved in binding to RNA G-quadruplex; 100..662; Interaction with GSK3B; 100..110; Interaction with IKBKE; 139..172; Interaction with CHUK; 250..259; Involved in stimulation of ATPase activity by DNA and RNA, nucleic acid binding and unwinding and HIV-1 replication; 409..662; Interaction with HCV core protein; 536..661; Interaction with NXF1; 601..634; Disordered

Protein Families (2)

DEAD box helicase family
DDX3/DED1 subfamily

Sequence Similarities

Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.

Clinical Relevance5

Disease Involvement (3)

Cancer-related genesDisease variantIntellectual disability

Antibody (3)

HPA001648 HPA005631 HPA059585

Interaction Protein (6)

ENSG00000088888ENSG00000106263ENSG00000114867ENSG00000120948ENSG00000184110ENSG00000263528

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications4

PMID	Title	Abstract
24657793	Plasma biomarker discovery in preeclampsia using a novel differential isolation technology for circulating extracellular vesicles.	No abstract available
37922300	Proteomic profiling of urinary extracellular vesicles differentiates breast cancer patients from healthy women.	No abstract available
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available
39873726	Size-Dependent Separation of Extracellular Vesicle Subtypes with Exodisc Enabling Proteomic Analysis in Prostate Cancer.	No abstract available