EV MemProt Atlas

Basic Information

Protein Names

Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF-1) (Ezrin-radixin-moesin-binding phosphoprotein 50) (EBP50) (Regulatory cofactor of Na(+)/H(+) exchanger) (Sodium-hydrogen exchanger regulatory factor 1) (Solute carrier family 9 isoform A3 regulatory factor 1)

Protein Class

Disease related genesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsTransporters

Protein Function

Human disease related genes:Urinary system diseases:Kidney diseases
Predicted intracellular proteins
Human disease related genes:Musculoskeletal diseases:Skeletal diseases
Potential drug targets
Transporters:Accessory Factors Involved in Transport
Disease related genes

Ensembl

ENSG00000109062

Entrez Gene Symbol

NHERF1

Gene Synonym

EBP50NHERFNHERF1

Gene Description

SLC9A3 regulator 1

Chromosome

17

Position

74748628-74769353

Frequency

1

EVMP Score

0.50

Fluorescence & Localization

Tissue Specificbone marrowCell SpecificcDCSingle-Nuclei Brain Specificendothelial cell

Function & Pathway

Protein Function

Human disease related genes:Urinary system diseases:Kidney diseases
Predicted intracellular proteins
Human disease related genes:Musculoskeletal diseases:Skeletal diseases
Potential drug targets
Transporters:Accessory Factors Involved in Transport
Disease related genes

Cellular Component

Molecular Function

Biological Process

KEGG

Canonical Pathways

M277 Pid integrin a4b1 pathway
M8626 Sig bcr signaling pathway
M18 Pid integrin1 pathway

Mediation Categories

Fusion and delivery mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

0 records.

Ligand-Receptor Signaling

9 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
extracellular	extracellular	DGIdb	No	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
apical_cell_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network

6 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
NHRF1	O14745	ADRB2	P07550	Yes	Yes	No	DOMINOWangSIGNORELMCui2007HINTHPRDBioGRIDCA1PDZBaseInnateDBLMPIDSPIKE_LCLit-BM-17	BioGRID:11882663 Lit-BM-17:9560162 LMPID:11882663 HPRD:9560162 CA1:11882663 BioGRID:9560162 DOMINO:9671706 LMPID:9671706 Lit-BM-17:9671706 HPRD:9671706 SIGNOR:9671706 Lit-BM-17:11882663 InnateDB:15452121 HINT:11882663 HINT:9560162 PDZBase:9671706 ELM:11882663 HINT:9671706 InnateDB:11882663 SPIKE_LC:9671706 LMPID:9560162 InnateDB:10499588 Lit-BM-17:11526121 HINT:10499588
GRK6	P43250	NHRF1	O14745	Yes	No	Yes	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteHPRD-phos	KEA:15302935 HPRD-phos:18088087 HPRD-phos:18669648 ProtMapper:19413330 ProtMapper:10446210 ProtMapper:15302935 phosphoELM:10446210 ProtMapper:18088087 PhosphoSite:30696771 HPRD-phos:18452278 SIGNOR:10446210 KEA:10446210 KEA:18088087 HPRD:10446210 HPRD-phos:15302935 ProtMapper:20166139 HPRD-phos:19413330 ProtMapper:18669648 ProtMapper:18452278 PhosphoSite:17895247 HPRD-phos:20166139 ProtMapper:19664995 HPRD-phos:19664995
KS6A1	Q15418	NHRF1	O14745	Yes	Yes	No	Sparser_ProtMapperSIGNORProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper	SIGNOR:26862730 ProtMapper:26862730
CDK1	P06493	NHRF1	O14745	Yes	No	Yes	Sparser_ProtMapperPhosphoSite_norefSIGNORiPTMnetProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:11533036 SIGNOR:20937695 ProtMapper:20937695 ProtMapper:15701524 PhosphoSite:30326259 ProtMapper:26862730 ProtMapper:22523604 PhosphoSite:23775624
KPCA	P17252	NHRF1	O14745	Yes	Yes	No	PhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:12881487 PhosphoSite:20571032 phosphoELM:12881487 PhosphoSite:17613530 KEA:12881487
AKT1	P31749	NHRF1	O14745	Yes	No	No	iPTMnetPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:25492869

Protein Complex Composition

9 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
NHERF1-PRKCZ complex	NHERF1 PRKCZ	O14745 Q05513	0:0	CORUM	CORUM:6790	24196963
SLC9A3R1-ACTN4 complex	ACTN4 NHERF1	O14745 O43707	0:0	CORUM	CORUM:6788	26432781
	NHERF1 PCYOX1 RNASET2 TACC1	O00584 O14745 O75410 Q9UHG3	0:0:0:0	Havugimana2012	Havugimana2012:C_344
	NHERF1	O14745	2	PDB	PDB:4q3hPDB:6rqr
	NHERF1 PDZK1 SLC22A5	O14745 O76082 Q5T2W1	1:1:1	CompleatCFinder	Compleat:HC8428
	ACSF3 CLEC4A DHFR NHERF1 NUTF2 UBQLN1 UBQLN4	O14745 P00374 P61970 Q4G176 Q9NRR5 Q9UMR7 Q9UMX0	0:0:0:0:0:0:0	hu.MAP2
	COX5A HMGN5 NHERF1	O14745 P20674 P82970	0:0:0	hu.MAP
	NHERF1 NUTF2 UBQLN1	O14745 P61970 Q9UMX0	0:0:0	hu.MAP2
	IRF2BP2 NHERF1 UBQLN1 ZMYM3 ZMYM4	O14745 Q14202 Q5VZL5 Q7Z5L9 Q9UMX0	0:0:0:0:0	hu.MAP2

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Western blotting	1	37922300

Sequence, Structure & Domains

Sequences

Length

358

Mass

38,868

Sequence

MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O14745-1; Sequence=Displayed; Name=2; IsoId=O14745-2; Sequence=VSP_055497

Alternative Sequence

1..156; Missing (in isoform 2)

3D Structural Models

Turn

93..95; 101..103; 106..108; 160..162; 222..224

Helix

47..50; 72..81; 109..112; 187..190; 212..221; 233..242; 249..252; 261..265; 297..299; 323..328; 329..333; 348..355

Beta Strand

13..18; 23..25; 27..30; 32..40; 58..62; 82..91; 115..118; 153..158; 166..170; 172..182; 184..186; 198..202; 225..231; 286..289; 300..302; 313..315

3D Structure

NMR spectroscopy (6); X-ray crystallography (14)

Domain & Motif Annotations

Compositional Bias

114..132; Low complexity; 135..152; Basic and acidic residues; 288..306; Polar residues; 307..319; Low complexity; 348..358; Basic and acidic residues

Domain (FT)

14..94; PDZ 1; 154..234; PDZ 2

Region

114..192; Disordered; 277..358; Disordered

Clinical Relevance

Disease Involvement

Disease variantOsteoporosis

Antibody

CAB001962 HPA009672 HPA027247

Interaction Protein

ENSG00000001626ENSG00000040199ENSG00000076641ENSG00000081913ENSG00000092820ENSG00000099992ENSG00000113721ENSG00000130234ENSG00000137693ENSG00000169252ENSG00000171862

Interaction Count

11

Interaction Dataset

intact_biogrid

Entry name NHRF1	UniProt ID O14745	EVMP score 0.50
Frequency 1	Transmembrane count	Protein classification Disease related genesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsTransporters

NHRF1