Protein detail

PLS1

Phospholipid scramblase 1 (PL scramblase 1) (Ca(2+)-dependent phospholipid scramblase 1) (Erythrocyte phospholipid scramblase) (Mg(2+)-dependent nuclease) (EC 3.1.-.-) (MmTRA1b)

Entry name PLS1	UniProt ID O15162	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 1	Protein classification Predicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Phospholipid scramblase 1 (PL scramblase 1) (Ca(2+)-dependent phospholipid scramblase 1) (Erythrocyte phospholipid scramblase) (Mg(2+)-dependent nuclease) (EC 3.1.-.-) (MmTRA1b)

Protein Class (2)

Predicted intracellular proteinsTransporters

Protein Function (2)

Transporters
Predicted intracellular proteins

Transmembrane

289..305; Helical

Transmembrane Count

Ensembl

ENSG00000188313

Entrez Gene Symbol

PLSCR1

Gene Synonym

MMTRA1B

Gene Description

Phospholipid scramblase 1

Chromosome

Position

146515180-146544856

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization4

Tissue Specificstomach 1Brain Regional SpecificcerebellumCell SpecificParietal cellsSingle-Nuclei Brain Specificcerebellar inhibitory

Function & Pathway5

Protein Function (2)

Transporters
Predicted intracellular proteins

Cellular Component (12)

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Molecular Function (15)

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Biological Process (3)

Mediation Categories (3)

Fusion and delivery mediationImmune mediationMetabolism mediation

Relations & Evidence27

Enzyme-Mediated Modification (6)

6 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PLSCR1	ABL1	P00519	Y	74	phosphorylation	BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperdbPTMKEAphosphoELMLi2012SIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11390389 ProtMapper:15212693 phosphoELM:11390389 ProtMapper:11390389 KEA:12871937 dbPTM:11390389 SIGNOR:11390389
PLSCR1	ABL1	P00519	Y	69	phosphorylation	BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperdbPTMKEAphosphoELMLi2012SIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11390389 ProtMapper:15212693 phosphoELM:11390389 ProtMapper:11390389 KEA:12871937 dbPTM:11390389 SIGNOR:11390389
PLSCR1	SRC	P12931	Y	74	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11390389 ProtMapper:12871937 SIGNOR:12871937 KEA:12871937 phosphoELM:12871937
PLSCR1	SRC	P12931	Y	69	phosphorylation	BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11390389 ProtMapper:15212693 SIGNOR:12871937 KEA:12871937 ProtMapper:12871937 phosphoELM:12871937
PLSCR1	PRKCD	Q05655	T	161	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapperPhosphoSite	ProtMapper:10770950 HPRD:10770950 KEA:10770950 SIGNOR:10770950
PLSCR1	CSK	P41240	Y	74	phosphorylation	Sparser_ProtMapperProtMapper	ProtMapper:23259795

Ligand-Receptor Signaling (14)

14 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	CellCellInteractions	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	UniProt_location	No	No	No	No	No
transmembrane	transmembrane	UniProt_topology	No	No	No	No	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No

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Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
SRC	P12931	PLS1	O15162	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEALi2012Cui2007CancerCellMapWangPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetKEAphosphoELMSIGNOR_ProtMapperSparser_ProtMapperSPIKE_LCSPIKE	KEA:11390389 ProtMapper:15212693 CancerCellMap:12871937 phosphoELM:12871937 SIGNOR:12871937 KEA:12871937 SPIKE:12871937 ProtMapper:12871937 ProtMapper:23259795 SPIKE_LC:12871937
ABL1	P00519	PLS1	O15162	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperdbPTMPhosphoSite_KEAphosphoELM_KEALi2012HPRDWangPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteSPIKE_LCSPIKE	KEA:11390389 ProtMapper:15212693 PhosphoSite:12871937 HPRD:11390389 SPIKE_LC:20841568 SPIKE:11390389 phosphoELM:11390389 PhosphoSite:11390389 SPIKE_LC:11390389 ProtMapper:11390389 dbPTM:11390389 KEA:12871937 SPIKE:20841568 PhosphoSite:32292520 SIGNOR:11390389
KPCD	Q05655	PLS1	O15162	Yes	Yes	No	Sparser_ProtMapperPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD:10770950 ProtMapper:29748552 HPRD-phos:10770950 ProtMapper:10770950 SIGNOR:10770950 KEA:10770950

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database
PLSCR1 SPRY2	O15162 O43597	0:0	hu.MAP
DYRK2 PLSCR1 RTN1 RTN2 SPRY2	O15162 O43597 O75298 Q16799 Q92630	0:0:0:0:0	hu.MAP2
PLSCR1 REEP1 REEP2 SPRY2	O15162 O43597 Q9BRK0 Q9H902	0:0:0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Size Exclusion Chromatography	Mass spectrometry	1	31414377

Sequence, Structure & Domains12

Sequences

Length

318

Mass

35,049

Sequence

MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O15162-1; Sequence=Displayed; Name=2; IsoId=O15162-2; Sequence=VSP_055237, VSP_055238

Alternative Sequence

1..23; MDKQNSQMNASHPETNLPVGYPP -> MLLTRKQTCQLGILLSIHRQHSK (in isoform 2); 24..104; Missing (in isoform 2)

3D Structural Models

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

1..14; Polar residues; 31..44; Low complexity

Motif

18..26; SH3-binding 1; 22..25; PPXY motif 1; 33..36; PPXY motif 2; 42..50; SH3-binding 2; 84..92; SH3-binding 3; 257..266; Nuclear localization signal

Domain (CC)

The N-terminal proline-rich domain (PRD) is required for phospholipid scramblase activity.; DOMAIN: The transmembrane domain is essential for membrane insertion, phospholipid scramblase activity and proper calcium-binding.

Region

1..84; Proline-rich domain (PRD); 1..64; Disordered; 99..290; Interaction with hepatitis C virus E2 glycoprotein

Protein Families

Phospholipid scramblase family

Sequence Similarities

Belongs to the phospholipid scramblase family.

Clinical Relevance4

Antibody (2)

HPA068923 CAB070421

Interaction Protein (10)

ENSG00000008405ENSG00000108010ENSG00000108256ENSG00000111605ENSG00000113905ENSG00000114354ENSG00000140009ENSG00000182944ENSG00000183283ENSG00000204099

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
32301581	Proteomic and biological profiling of extracellular vesicles from Alzheimer's disease human brain tissues.	Extracellular vesicles (EVs) from human Alzheimer's disease (AD) biospecimens contain amyloid beta (Aβ) peptide and tau.