Protein detail

BCAR3

Breast cancer anti-estrogen resistance protein 3 (Novel SH2-containing protein 2) (SH2 domain-containing protein 3B)

Protein symbol BCAR3	UniProt ID O75815	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Breast cancer anti-estrogen resistance protein 3 (Novel SH2-containing protein 2) (SH2 domain-containing protein 3B)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000137936

Entrez Gene Symbol

BCAR3

Gene Synonym

AND-34MIG7NSP2SH2D3B

Gene Description

BCAR3 adaptor protein, NSP family member

Chromosome

Position

93561741-93847150

Frequency

EVMP Score

0.38

Fluorescence & Localization

Tissue Specificheart muscleCell SpecificAstrocytesSingle-Nuclei Brain Specificpericyte

Function & Pathway

Protein Function

Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

Mediation Categories

Adhesion and uptake mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

0 records.

Ligand-Receptor Signaling

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network

0 records.

Protein Complex Composition

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	BCAR3	O75815	4	PDB	PDB:3t6a

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryWestern blotting	3	27086912 33893753 38716512

Sequence, Structure & Domains

Sequences

Length

825

Mass

92,566

Sequence

MAAGKFASLPRNMPVNHQFPLASSMDLLSSRSPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDDFSHMGTLPHSKSPRQNSPVTQDGIQESPWQDRHGETFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKKELEEELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTKGRPDVAKRLSLTMGGVQAREQNLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPPQSSGVDTSPCPNSPVFRTGSEPALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPSAWLNSEANYCELNPAFATGCGRGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPAAAQMEKGQWDKGEFVTPLLETVSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMDCRVARILGVSEEMRRNMGVSSGLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKIIQVAVELKDSMGDLYSFSALMKALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCVPPNNVSVPLLMPLVTLMERQAVTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQPDEEMNEICKTEFQMRLLWGSKGAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=O75815-1; Sequence=Displayed; Name=2; IsoId=O75815-2; Sequence=VSP_017814; Name=3; IsoId=O75815-3; Sequence=VSP_046716, VSP_046717

Alternative Sequence

1..324; Missing (in isoform 2); 1..91; Missing (in isoform 3); 92..117; ESPWQDRHGETFTFRDPHLLDPTVEY -> MPKECSAFHALSAALCCFYHRKSFIG (in isoform 3)

3D Structural Models

Turn

765..767

Helix

517..519; 534..546; 549..563; 571..577; 582..585; 589..591; 592..614; 620..639; 644..655; 657..660; 663..672; 674..682; 684..692; 696..698; 711..717; 724..726; 732..734; 737..752; 754..764; 772..777; 780..792; 797..815

Beta Strand

578..580

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

79..94; Polar residues; 95..106; Basic and acidic residues

Domain (CC)

The SH2 domain mediates interaction with tyrosine-phosphorylated proteins (PubMed:18722344). However, not involved in the binding to phosphorylated BCAR1 (PubMed:18722344). Required for cell cycle progression in response to INS/insulin (PubMed:24216110). Required for regulation of EFR-induced DNA synthesis (PubMed:18722344).; DOMAIN: The Ras-GEF domain appears to adopt a closed conformation rendering it incapable of carrying out canonical exchange factor function, this closed conformation is probably required for interaction with BCAR1..

Domain (FT)

154..253; SH2; 548..818; Ras-GEF

Region

40..106; Disordered; 744..748; Mediates the interaction with BCAR1/p130CAS

Clinical Relevance

Drugs

ANTIESTROGEN

Antibody

HPA014858

Interaction Protein

ENSG00000050820ENSG00000111859ENSG00000140961ENSG00000169047

Interaction Count

Interaction Dataset

biogrid_opencell_bioplexintact_biogrid