Protein detail

FLOT1

Flotillin-1

Entry name FLOT1	UniProt ID O75955	EVMP confidence score 0.38
Supporting publications (n) 4	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Flotillin-1

Protein Class (3)

Plasma proteinsPredicted intracellular proteinsTransporters

Protein Function (2)

Predicted intracellular proteins
Transporters:Accessory Factors Involved in Transport

Ensembl

ENSG00000137312

Entrez Gene Symbol

FLOT1

Gene Description

Flotillin 1

Chromosome

Position

30727709-30742732

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Cell SpecificChoroid plexus epithelial cellsSingle-Nuclei Brain Specificendothelial cell

Function & Pathway7

Protein Function (2)

Predicted intracellular proteins
Transporters:Accessory Factors Involved in Transport

Cellular Component (28)

Page 1 of 3

Molecular Function (3)

Biological Process (3)

KEGG

hsa04910 Insulin signaling pathway

Reactome (11)

Page 1 of 2

Mediation Categories (5)

Adhesion and uptake mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence25

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
FLOT1	SRC	P12931	Y	56	phosphorylation	Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:26923594
FLOT1	SRC	P12931	Y	149	phosphorylation	Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:26923594
FLOT1	FYN	P06241	Y	160	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
FLOT1	PRKCA	P17252	S	315	phosphorylation	PhosphoSite

Ligand-Receptor Signaling (15)

15 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
extracellular	extracellular	DGIdb	No	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
flotillin	intracellular_intercellular_related	HGNC	No	Yes	No	No	No
intracellular_intercellular_related	intracellular_intercellular_related	OmniPath	Yes	No	No	No	No

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Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
SRC	P12931	FLOT1	O75955	Yes	Yes	No	Sparser_ProtMapperPhosphoSite_norefSIGNORiPTMnetProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:24983503 ProtMapper:26923594 SIGNOR:24983503
FYN	P06241	FLOT1	O75955	Yes	No	No	iPTMnetPhosphoPointProtMapperHPRDREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:19258392 ProtMapper:32297092 HPRD:11598189
KPCA	P17252	FLOT1	O75955	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:21399631 PhosphoSite:31753918

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	FLOT1 J3QLD9	J3QLD9 O75955	1:1	PDB	PDB:9bq2
	ATP6V0A1 FLOT1	O75955 Q93050	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometryWestern blottingELISA	2	38590132 38716512

Sequence, Structure & Domains8

Sequences

Length

427

Mass

47,355

Sequence

MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O75955-1; Sequence=Displayed; Name=2; IsoId=O75955-2; Sequence=VSP_056227

Alternative Sequence

71..118; Missing (in isoform 2)

3D Structural Models

3D Structure

Electron microscopy (1)

Domain & Motif Annotations

Protein Families (2)

Band 7/mec-2 family
Flotillin subfamily

Sequence Similarities

Belongs to the band 7/mec-2 family. Flotillin subfamily.

Clinical Relevance4

Antibody (2)

HPA001393 CAB007766

Interaction Protein (4)

ENSG00000111665ENSG00000132589ENSG00000141469ENSG00000176105

Interaction Count

Interaction Dataset (2)

biogrid_bioplexintact_biogrid

Supporting Publications4

PMID	Title	Abstract
23585443	Proteome profiling of exosomes derived from human primary and metastatic colorectal cancer cells reveal differential expression of key metastatic factors and signal transduction components.	A key finding of this study was the detection and colocalization of protein complexes EPCAM-CLDN7 and TNIK-RAP2A in colorectal cancer cell exosomes. A major finding was the selective enrichment of metastatic factors (MET, S100A8, S100A9, TNC), signal transduction molecules (EFNB2, JAG1, SRC, TNIK), and lipid raft and lipid raft-associated components (CAV1, FLOT1, FLOT2, PROM1) in exosomes derived from metastatic SW620 cells. Exosomes purified using OptiPrep™ density gradient fractionation were 40-100 nm in diameter, were of a buoyant density ~1.09 g/mL, and displayed stereotypic exosomal markers TSG101, Alix, and CD63.
27797340	Sorting protein VPS33B regulates exosomal autocrine signaling to mediate hematopoiesis and leukemogenesis.	Deletion of VPS33B in either mouse or human HSCs resulted in impaired exosome maturation and secretion as well as loss of stemness. Mechanistically, VPS33B interacted with the GDI2/RAB11A/RAB27A pathway to regulate the trafficking of secretory proteins as exosomes. These findings reveal an essential role for VPS33B in exosome pathways in HSCs and LICs. VPS33B co-existed in exosomes with GDI2, VPS16B, FLOT1, and other known exosome markers.
37536457	Blood exosome marker miRNA-30d-5p: Role and regulation mechanism in cell stemness and gemcitabine resistance of hepatocellular carcinoma.	Blood exosome-derived miRNA-30d-5p promoted the stemness and gemcitabine resistance of HCC cells by repressing SOCS3 expression.
38199177	BeWo exomeres are enriched for bioactive extracellular placenta-specific C19MC miRNAs.	Exomeres were positive for AGO2 but negative for exosome markers (CD63, CD9, CD81, FLOT1, and TSG101).