Protein detail
GRP1
RAS guanyl-releasing protein 1 (Calcium and DAG-regulated guanine nucleotide exchange factor II) (CalDAG-GEFII) (Ras guanyl-releasing protein)
Entry name GRP1 | UniProt ID | EVMP confidence score 0.38 |
Supporting publications (n) 1 | Transmembrane count | Protein classification |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information6
Protein Names
RAS guanyl-releasing protein 1 (Calcium and DAG-regulated guanine nucleotide exchange factor II) (CalDAG-GEFII) (Ras guanyl-releasing protein)
Protein Function (4)
- Human disease related genes:Immune system diseases:Primary immunodeficiency
- Disease related genes
- Predicted intracellular proteins
- RAS pathway related proteins
Ensembl
Entrez Gene Symbol
Supporting publications (n)
1
EVMP confidence score
0.38
Fluorescence & Localization3
Tissue SpecificretinaCell SpecificAstrocytesSingle-Nuclei Brain SpecificBergmann glia
Function & Pathway7
Protein Function (4)
- Human disease related genes:Immune system diseases:Primary immunodeficiency
- Disease related genes
- Predicted intracellular proteins
- RAS pathway related proteins
Cellular Component (6)
Molecular Function (7)
Biological Process (3)
KEGG (6)
Reactome (17)
- R-hsa-1169092 activation of ras in b cells
- R-hsa-1280218 adaptive immune system
- R-hsa-1168372 downstream signaling events of b cell receptor bcr
- R-hsa-114508 effects of pip2 hydrolysis
- R-hsa-2871837 fceri mediated nf kb activation
- R-hsa-2454202 fc epsilon receptor fceri signaling
- R-hsa-416476 g alpha q signalling events
- R-hsa-109582 hemostasis
- R-hsa-168249 innate immune system
- R-hsa-354192 integrin signaling
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Mediation Categories (4)
Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation
Relations & Evidence15
Enzyme-Mediated Modification (1)
1 record.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| RASGRP1 | PRKCQ | Q04759 | T | 184 | phosphorylation | PhosphoSite_MIMPMIMPNCI-PID_ProtMapperProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:15899849ProtMapper:9819387 |
Ligand-Receptor Signaling (9)
9 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
Regulatory Interaction Network (1)
1 record.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| KPCT | Q04759 | GRP1 | O95267 | Yes | Yes | No | PhosphoSite_MIMPMIMPNCI-PID_ProtMapperiPTMnetProtMapperWangPhosphoSitePhosphoSite_ProtMapper | ProtMapper:15899849PhosphoSite:15899849ProtMapper:9819387 |
Protein Complex Composition (3)
3 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| DGKZ-RASGRP1-HRAS complex | DGKZHRASRASGRP1 | O95267P01112Q13574 | 0:0:0 | CORUM | CORUM:6219 | 11257115 |
| RASGRP1 | O95267 | 2 | PDB | PDB:4l9u | ||
| ABCB8ADPGKLDHAL6BRAB11FIP5RASGRP1 | O95267Q9BRR6Q9BXF6Q9BYZ2Q9NUT2 | 0:0:0:0:0 | Havugimana2012 | Havugimana2012:C_429 |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Western BlottingMass SpectrometryFlow Cytometry | 1 | 38207106 |
Sequence, Structure & Domains17
Sequences
Length
797
Mass
90,402
Sequence
MGTLGKAREAPRKPSHGCRAASKARLEAKPANSPFPSHPSLAHITQFRMMVSLGHLAKGASLDDLIDSCIQSFDADGNLCRSNQLLQVMLTMHRIVISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWVMFKMDASLTDTMEEFQELVKAKGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTYLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSSRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEDGKVNVHKLLALYNHISELVQLQEVAPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHRAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNPVAPTENNTSVGPVSNLCSLGAKDLLHAPEEGPFTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKATQTESQPWIGSEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLKYAQKKIESLQLEKSNHVLAQMEQGDCS
Alternative Products
Event=Alternative splicing; Named isoforms=5; Comment=Additional isoforms seem to exist. Several splicing events may be used independently in a modular way.; Name=1; IsoId=O95267-1; Sequence=Displayed; Name=2; Synonyms=A; IsoId=O95267-2; Sequence=VSP_030836; Name=3; Synonyms=B; IsoId=O95267-3; Sequence=VSP_030836, VSP_030841, VSP_030842; Name=4; Synonyms=C; IsoId=O95267-4; Sequence=VSP_030836, VSP_030837, VSP_030838; Name=5; Synonyms=D; IsoId=O95267-5; Sequence=VSP_030836, VSP_030839, VSP_030840
Alternative Sequence
442..476; Missing (in isoform 2, isoform 3, isoform 4 and isoform 5); 513..516; REGL -> SSGE (in isoform 4); 517..797; Missing (in isoform 4); 574..581; DCGMNCHK -> GNKYSESR (in isoform 5); 582..797; Missing (in isoform 5); 625..632; APEEGPFT -> GNKYSESR (in isoform 3); 633..797; Missing (in isoform 3)
3D Structural Models
Turn
267..269; 335..337; 556..558; 573..575; 581..583; 783..785
Helix
62..71; 84..92; 93..95; 99..115; 119..135; 138..142; 144..160; 164..168; 172..179; 206..222; 226..234; 242..264; 270..287; 291..301; 304..307; 310..315; 318..331; 338..345; 355..368; 380..395; 396..398; 407..416; 423..433; 464..482; 492..500; 519..533; 748..782; 788..791
Beta Strand
201..203; 376..378; 544..547; 564..566; 569..572; 584..586
3D Structure
X-ray crystallography (2)
Domain & Motif Annotations
Compositional Bias
1..12; Basic and acidic residues
Coiled Coil
746..786
Zinc Finger
541..591; Phorbol-ester/DAG-type
Domain (CC)
The phorbol-ester/DAG-type zinc finger is the principal mediator of the targeting to membranes and is required for functional activation through DAG-binding.; DOMAIN: Two EF-hand domains are present. However, only EF-hand 1 (and not EF-hand 2) binds calcium.
Domain (FT)
53..176; N-terminal Ras-GEF; 205..436; Ras-GEF; 470..505; EF-hand 1; 506..532; EF-hand 2
Region
1..23; Disordered; 57..110; Ras exchanger motif region; required for transforming activity; 673..694; Disordered; 686..694; Suppress the PT region-mediated translocation to plasma membrane; 718..797; PT region; mediates the BCR-dependent translocation to plasma membrane
Protein Families
RASGRP family
Sequence Similarities
Belongs to the RASGRP family.
Clinical Relevance1
Supporting Publications1
| PMID | Title | Abstract |
|---|---|---|
| 31805958 | Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment. | No abstract available |