EV MemProt Atlas

Basic Information

Protein Names

Antiviral innate immune response receptor RIG-I (ATP-dependent RNA helicase DDX58) (EC 3.6.4.13) (DEAD box protein 58) (RIG-I-like receptor 1) (RLR-1) (RNA sensor RIG-I) (Retinoic acid-inducible gene 1 protein) (RIG-1) (Retinoic acid-inducible gene I protein) (RIG-I)

Protein Class

Disease related genesEnzymesHuman disease related genesPotential drug targetsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins
Human disease related genes:Congenital malformations:Congenital malformations of the musculoskeletal system
Potential drug targets
Enzymes
ENZYME proteins:Hydrolases
Disease related genes

Ensembl

ENSG00000107201

Entrez Gene Symbol

RIGI

Gene Synonym

DDX58DKFZp434J1111FLJ13599RIG-1RIG-IRIG1

Gene Description

RNA sensor RIG-I

Chromosome

9

Position

32455302-32526208

Frequency

3

EVMP Score

0.50

Fluorescence & Localization

Function & Pathway

Protein Function

Predicted intracellular proteins
Human disease related genes:Congenital malformations:Congenital malformations of the musculoskeletal system
Potential drug targets
Enzymes
ENZYME proteins:Hydrolases
Disease related genes

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Canonical Pathways

M198 Pid syndecan 1 pathway

Mediation Categories

Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediation

Relations & Evidence

Enzyme-Mediated Modification

20 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
RIGI	DAPK1	P53355	T	671	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	DAPK1	P53355	S	764	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	DAPK1	P53355	T	770	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	DAPK1	P53355	S	8	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	DAPK1	P53355	T	674	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	DAPK1	P53355	T	667	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	IKBKE	Q14164	S	855	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	PRKCA	P17252	T	170	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	PRKCA	P17252	S	8	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
RIGI	VCL	P18206	S	8	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:25011106

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Ligand-Receptor Signaling

9 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
tight_junction	tight_junction	GO_Intercell	Yes	Yes	No	No	No
tight_junction	tight_junction	OmniPath	Yes	Yes	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

14 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
RIGI	O95786	MAVS	Q7Z434	Yes	Yes	Yes	WangMacrophageKEGG-MEDICUSSIGNORHPRDHINTBioGRIDIntActInnateDBDIPSPIKE_LCSPIKE	InnateDB:18207245 InnateDB:19881509 HINT:25018021 Macrophage:16301520 HINT:34544279 HINT:22844514 InnateDB:21200404 HINT:33976430 BioGRID:20554965 BioGRID:16177806 InnateDB:21102435 HINT:17709747 BioGRID:29263274 IntAct:19193793 IntAct:20007272 DIP:21844353 InnateDB:23843640 HINT:21844353 InnateDB:22844514 HPRD:16177806 IntAct:17190814 IntAct:26494172 DIP:26494172 HINT:20080758 InnateDB:16127453 InnateDB:18977754 IntAct:17709747 IntAct:22301138 InnateDB:21844353 BioGRID:37870297 DIP:16585524 HINT:19036819 DIP:17190814 BioGRID:33976430 SPIKE:16177806 HINT:24478431 HINT:33372174 Macrophage:16125763 DIP:18200010 HINT:18948594 IntAct:33976430 Macrophage:16127453 HINT:21903422 HINT:22301138 HINT:19193793 DIP:17709747 Macrophage:16713980 InnateDB:21903422 SPIKE_LC:17145710 HINT:16177806 HINT:19164550 BioGRID:34544279 HINT:18977754 HINT:20007272 InnateDB:16153868 Macrophage:16153868 IntAct:34544279 HINT:16153868 HINT:21102435 SPIKE_LC:16177806 HINT:17190814 DIP:19164550 HINT:35075101 HINT:17020950 DIP:20080758 HINT:21419663 InnateDB:19036819 Macrophage:17020950 InnateDB:20406818 IntAct:35075101 InnateDB:21419663 InnateDB:17190814 IntAct:21102435 InnateDB:18948594 IntAct:24478431 IntAct:18200010 HINT:16585524 IntAct:19164550 HINT:18200010 InnateDB:23499489 InnateDB:16585524 InnateDB:17020950 HINT:16127453 IntAct:16177806 InnateDB:16177806 IntAct:16585524 Macrophage:16177806 IntAct:33372174 HINT:18207245 HINT:26494172 HINT:20406818 Macrophage:16785313 SIGNOR:19052324 HINT:19881509
RN135	Q8IUD6	RIGI	O95786	Yes	Yes	No	SIGNORHINTBioGRIDInnateDBSPIKE_LCLit-BM-17SPIKE	HINT:33536170 HINT:19484123 InnateDB:23950712 HINT:37951994 Lit-BM-17:19017631 Lit-BM-17:19484123 HINT:33373584 BioGRID:35089988 SIGNOR:19017631 SPIKE_LC:19017631 SPIKE:19017631 HINT:31006531 Lit-BM-17:23950712 InnateDB:19484123 HINT:19017631 InnateDB:19017631
TRI25	Q14258	RIGI	O95786	Yes	Yes	No	WangMacrophageKEGG-MEDICUSSIGNORHINTBioGRIDIntActInnateDBSPIKE_LCSPIKE	HINT:19675569 SIGNOR:17392790 HINT:25172487 IntAct:34471099 InnateDB:20406818 HINT:34471099 HINT:20818395 IntAct:35075101 BioGRID:37628607 InnateDB:23950712 HINT:34452305 InnateDB:18948594 BioGRID:36975005 HINT:31600868 InnateDB:20818395 SIGNOR:24493797 IntAct:24478431 InnateDB:17392790 BioGRID:32513696 Macrophage:17392790 SPIKE_LC:18353649 BioGRID:32295922 HINT:23264040 SPIKE:18353649 HINT:24478431 BioGRID:36146771 BioGRID:33770145 BioGRID:34452305 HINT:17392790 HINT:35075101 HINT:18948594 BioGRID:30902577 BioGRID:27122586 BioGRID:34529741 HINT:20406818
RN125	Q96EQ8	RIGI	O95786	Yes	No	Yes	MacrophageKEGG-MEDICUSSIGNORHINTInnateDBWang	HINT:17460044 SIGNOR:17460044 HINT:26471729 InnateDB:17460044 Macrophage:17460044
CSK21	P68400	RIGI	O95786	Yes	No	Yes	PhosphoSite_MIMPMIMPPhosphoSite_norefSIGNORiPTMnetProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:26354181 SIGNOR:21068236 ProtMapper:21068236
TERA	P55072	RIGI	O95786	Yes	No	Yes	SIGNOR	SIGNOR:26471729
DAPK1	P53355	RIGI	O95786	Yes	No	Yes	Sparser_ProtMapperPhosphoSite_norefSIGNORProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:30985869 PhosphoSite:28132841 PhosphoSite:20406818 ProtMapper:30985869 SIGNOR:28132841 ProtMapper:33462384 PhosphoSite:21068236
TRI58	Q8NG06	RIGI	O95786	Yes	Yes	No	SIGNOR	SIGNOR:23499489
	COMPLEX:Q96EP0_Q9BYM8	RIGI	O95786	Yes	No	Yes	SIGNOR	SIGNOR:21292167
CSK22	P19784	RIGI	O95786	Yes	No	Yes	SIGNOR	SIGNOR:21068236

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Protein Complex Composition

5 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
Ubiquitin E3 ligase (TRIM25DDX58)	RIGI TRIM25	O95786 Q14258	1:1	CompleatCORUM	Compleat:HC525CORUM:2717	17392790
	NSMCE1 NSMCE2 NSMCE3 NSMCE4A PDE6D RIGI SMC5 SMC6	O43924 O95786 Q8IY18 Q8WV22 Q96MF7 Q96MG7 Q96SB8 Q9NXX6	1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7679
	RIGI	O95786	10	PDB	PDB:3lrnPDB:7baiPDB:5f9hPDB:3ncuPDB:5f98PDB:7bahPDB:4on9PDB:3lrrPDB:3og8PDB:6kyvPDB:5f9fPDB:2qfdPDB:7mk1PDB:2qfb
	RIGI UBC	O95786 P0CG48	4:6	PDB	PDB:4nqk
	MAVS RIGI UBA52	O95786 P62987 Q7Z434	8:5:8	PDB	PDB:4p4h

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Western BlottingMass SpectrometryFlow Cytometry	1	38207106

Sequence, Structure & Domains

Sequences

Length

925

Mass

106,600

Sequence

MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O95786-1; Sequence=Displayed; Name=2; IsoId=O95786-2; Sequence=VSP_016054

Alternative Sequence

36..80; Missing (in isoform 2)

3D Structural Models

Turn

26..32; 83..85; 183..185; 314..318; 811..813; 827..829; 867..869; 897..899

Helix

2..11; 13..19; 22..25; 35..48; 50..63; 69..80; 86..91; 95..99; 101..117; 120..127; 128..130; 133..146; 148..160; 167..177; 245..255; 270..284; 300..313; 334..339; 348..356; 363..365; 374..376; 382..395; 420..433; 446..452; 470..489; 493..495; 507..518; 531..557; 560..575; 581..591; 594..602; 604..606; 609..622; 637..649; 651..653; 675..683; 706..708; 721..726; 727..731; 745..768; 773..793; 820..822; 838..840; 889..891; 908..910; 920..922

Beta Strand

260..263; 265..267; 293..296; 321..324; 326..328; 330..332; 342..346; 358..360; 367..372; 378..381; 396..398; 404..410; 438..440; 443..445; 457..462; 496..498; 504..506; 526..528; 630..633; 658..660; 667..670; 686..690; 694..700; 701..703; 710..716; 733..735; 737..743; 801..804; 806..810; 816..819; 823..826; 830..833; 835..837; 842..846; 853..855; 856..865; 872..879; 882..887; 892..896; 902..904

3D Structure

Electron microscopy (16); NMR spectroscopy (3); X-ray crystallography (23)

Domain & Motif Annotations

Motif

372..375; DECH box

Domain (CC)

The RLR CTR domain controls homooligomerization and interaction with MAVS/IPS1. In the absence of viral infection, the protein is maintained as a monomer in an autoinhibited state with the CARD domains masked through intramolecular interactions with the RLR CTR domain. Upon binding to viral RNA and ubiquitination by RNF135, a conformational change releases the autoinhibition promoting further homooligomerization, interaction of the CARD domains with the adapter protein MAVS/IPS1 and activation of the downstream RIG-I signaling pathway.; DOMAIN: The helicase domain is responsible for dsRNA recognition.; DOMAIN: The 2 CARD domains are responsible for interaction with and signaling through MAVS/IPS1 and for association with the actin cytoskeleton.; DOMAIN: The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.

Domain (FT)

1..87; CARD 1; 92..172; CARD 2; 251..430; Helicase ATP-binding; 610..776; Helicase C-terminal; 794..925; RLR CTR

Region

218..925; Interaction with ZC3HAV1; 735..925; Mediates interaction with RNF135

Protein Families

Helicase family
RLR subfamily

Sequence Similarities

Belongs to the helicase family. RLR subfamily.

Clinical Relevance

Disease Involvement

Disease variant

Drug Targets

Clinical trial target

Drugs

INARIGIVIR SOPROXIL NULL

Antibody

CAB012643 HPA047193 HPA073217

Interaction Protein

ENSG00000088888ENSG00000101695ENSG00000121060ENSG00000124535

Interaction Count

4

Interaction Dataset

intact_biogrid

Protein symbol RIGI	UniProt ID O95786	EVMP score 0.50
Frequency 3	Transmembrane count	Protein classification Disease related genesEnzymesHuman disease related genesPotential drug targetsPredicted intracellular proteins