Protein detail
RIGI
Antiviral innate immune response receptor RIG-I (ATP-dependent RNA helicase DDX58) (EC 3.6.4.13) (DEAD box protein 58) (RIG-I-like receptor 1) (RLR-1) (RNA sensor RIG-I) (Retinoic acid-inducible gene 1 protein) (RIG-1) (Retinoic acid-inducible gene I protein) (RIG-I)
Protein symbol RIGI | UniProt ID | EVMP score 0.50 |
Frequency 3 | Transmembrane count | Protein classification Disease related genesEnzymesHuman disease related genesPotential drug targetsPredicted intracellular proteins |
Basic Information
Protein Names
Antiviral innate immune response receptor RIG-I (ATP-dependent RNA helicase DDX58) (EC 3.6.4.13) (DEAD box protein 58) (RIG-I-like receptor 1) (RLR-1) (RNA sensor RIG-I) (Retinoic acid-inducible gene 1 protein) (RIG-1) (Retinoic acid-inducible gene I protein) (RIG-I)
Protein Class
Disease related genesEnzymesHuman disease related genesPotential drug targetsPredicted intracellular proteins
Protein Function
- Predicted intracellular proteins
- Human disease related genes:Congenital malformations:Congenital malformations of the musculoskeletal system
- Potential drug targets
- Enzymes
- ENZYME proteins:Hydrolases
- Disease related genes
Ensembl
Entrez Gene Symbol
Gene Synonym
DDX58DKFZp434J1111FLJ13599RIG-1RIG-IRIG1
Gene Description
RNA sensor RIG-I
Chromosome
9
Position
32455302-32526208
Frequency
3
EVMP Score
0.50
Fluorescence & Localization
Function & Pathway
Protein Function
- Predicted intracellular proteins
- Human disease related genes:Congenital malformations:Congenital malformations of the musculoskeletal system
- Potential drug targets
- Enzymes
- ENZYME proteins:Hydrolases
- Disease related genes
Cellular Component
Molecular Function
- GO:0003690 double-stranded DNA binding
- GO:0003724 RNA helicase activity
- GO:0003725 double-stranded RNA binding
- GO:0003727 single-stranded RNA binding
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0005525 GTP binding
- GO:0008270 zinc ion binding
- GO:0016887 ATP hydrolysis activity
- GO:0031625 ubiquitin protein ligase binding
- GO:0038187 pattern recognition receptor activity
- GO:0042802 identical protein binding
Biological Process
KEGG
- hsa04064 NF-kappa B signaling pathway
- KEGG:hsa04622 RIG-I-like receptor signaling pathway
- KEGG:hsa04623 Cytosolic DNA-sensing pathway
- KEGG:hsa05160 Hepatitis C
- KEGG:hsa05161 Hepatitis B
- KEGG:hsa05162 Measles
- KEGG:hsa05164 Influenza A
- KEGG:hsa05168 Herpes simplex virus 1 infection
- KEGG:hsa05169 Epstein-Barr virus infection
- KEGG:hsa05171 Coronavirus disease
Reactome
- R-hsa-1169410 antimicrobial mechanism of ifn stimulated genes
- R-hsa-1280215 cytokine signaling in immune system
- R-hsa-168928 ddx58 ifih1 mediated induction of interferon alpha beta
- R-hsa-5688426 deubiquitination
- R-hsa-9833109 evasion by rsv of host interferon responses
- R-hsa-5663205 infectious disease
- R-hsa-168249 innate immune system
- R-hsa-913531 interferon signaling
- R-hsa-1169408 isg15 antiviral mechanism
- R-hsa-9909505 modulation of host responses by ifn stimulated genes
- R-hsa-936440 negative regulators of ddx58 ifih1 signaling
- R-hsa-933543 nf kb activation through fadd rip 1 pathway mediated by caspase 8 and 10
- R-hsa-8983711 oas antiviral response
- R-hsa-5689896 ovarian tumor domain proteases
- R-hsa-597592 post translational protein modification
- R-hsa-9820952 respiratory syncytial virus infection pathway
- R-hsa-9833110 rsv host interactions
- R-hsa-9692916 sars cov 1 activates modulates innate immune responses
- R-hsa-9692914 sars cov 1 host interactions
- R-hsa-9678108 sars cov 1 infection
- R-hsa-9705671 sars cov 2 activates modulates innate and adaptive immune responses
- R-hsa-9705683 sars cov 2 host interactions
- R-hsa-9694516 sars cov 2 infection
- R-hsa-9679506 sars cov infections
- R-hsa-918233 traf3 dependent irf activation pathway
- R-hsa-933541 traf6 mediated irf7 activation
- R-hsa-933542 traf6 mediated nf kb activation
- R-hsa-5689880 ub specific processing proteases
- R-hsa-9824446 viral infection pathways
Canonical Pathways
M198 Pid syndecan 1 pathway
Mediation Categories
Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediation
Relations & Evidence
Enzyme-Mediated Modification
20 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| RIGI | RNF135 | Q8IUD6 | K | 907 | ubiquitination | SIGNOR | SIGNOR:19017631 |
| RIGI | RNF135 | Q8IUD6 | K | 909 | ubiquitination | SIGNOR | SIGNOR:19017631 |
| RIGI | PPP1CC | P36873 | S | 8 | dephosphorylation | SIGNOR | SIGNOR:23499489 |
| RIGI | PPP1CA | P62136 | S | 8 | dephosphorylation | SIGNOR | SIGNOR:23499489 |
| RIGI | TRIM58 | Q8NG06 | K | 172 | ubiquitination | SIGNOR | SIGNOR:23499489 |
| RIGI | CSNK2A1 | P68400 | S | 855 | phosphorylation | PhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:21068236ProtMapper:21068236 |
| RIGI | CSNK2A1 | P68400 | S | 854 | phosphorylation | PhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:21068236ProtMapper:21068236 |
| RIGI | CSNK2A1 | P68400 | T | 770 | phosphorylation | PhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:21068236ProtMapper:21068236 |
| RIGI | VCP | P55072 | K | 181 | ubiquitination | SIGNOR | SIGNOR:26471729 |
| RIGI | DAPK1 | P53355 | S | 654 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper |
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Ligand-Receptor Signaling
9 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| tight_junction | tight_junction | GO_Intercell | Yes | Yes | No | No | No |
| tight_junction | tight_junction | OmniPath | Yes | Yes | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
Regulatory Interaction Network
14 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| G3BP1 | Q13283 | RIGI | O95786 | Yes | No | Yes | InnateDBSIGNOR | InnateDB:22912779SIGNOR:30804210 |
| PP1G | P36873 | RIGI | O95786 | Yes | Yes | No | SIGNOR_ProtMapperSIGNORProtMapper | SIGNOR:23499489ProtMapper:23499489 |
| PP1A | P62136 | RIGI | O95786 | Yes | Yes | No | SIGNOR_ProtMapperSIGNORProtMapper | SIGNOR:23499489ProtMapper:23499489 |
| KPCA | P17252 | RIGI | O95786 | Yes | No | No | iPTMnetPhosphoSitePhosphoSite_ProtMapperProtMapper | PhosphoSite:23063562PhosphoSite:20071582PhosphoSite:23499489PhosphoSite:28250012PhosphoSite:22114345PhosphoSite:26471729PhosphoSite:23264040 |
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Protein Complex Composition
5 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| Ubiquitin E3 ligase (TRIM25DDX58) | RIGITRIM25 | O95786Q14258 | 1:1 | CompleatCORUM | Compleat:HC525CORUM:2717 | 17392790 |
| NSMCE1NSMCE2NSMCE3NSMCE4APDE6DRIGISMC5SMC6 | O43924O95786Q8IY18Q8WV22Q96MF7Q96MG7Q96SB8Q9NXX6 | 1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC7679 | ||
| RIGI | O95786 | 10 | PDB | PDB:3lrnPDB:7baiPDB:5f9hPDB:3ncuPDB:5f98PDB:7bahPDB:4on9PDB:3lrrPDB:3og8PDB:6kyvPDB:5f9fPDB:2qfdPDB:7mk1PDB:2qfb | ||
| RIGIUBC | O95786P0CG48 | 4:6 | PDB | PDB:4nqk | ||
| MAVSRIGIUBA52 | O95786P62987Q7Z434 | 8:5:8 | PDB | PDB:4p4h |
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Western BlottingMass SpectrometryFlow Cytometry | 1 | 38207106 |
Sequence, Structure & Domains
Sequences
Length
925
Mass
106,600
Sequence
MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O95786-1; Sequence=Displayed; Name=2; IsoId=O95786-2; Sequence=VSP_016054
Alternative Sequence
36..80; Missing (in isoform 2)
3D Structural Models
Turn
26..32; 83..85; 183..185; 314..318; 811..813; 827..829; 867..869; 897..899
Helix
2..11; 13..19; 22..25; 35..48; 50..63; 69..80; 86..91; 95..99; 101..117; 120..127; 128..130; 133..146; 148..160; 167..177; 245..255; 270..284; 300..313; 334..339; 348..356; 363..365; 374..376; 382..395; 420..433; 446..452; 470..489; 493..495; 507..518; 531..557; 560..575; 581..591; 594..602; 604..606; 609..622; 637..649; 651..653; 675..683; 706..708; 721..726; 727..731; 745..768; 773..793; 820..822; 838..840; 889..891; 908..910; 920..922
Beta Strand
260..263; 265..267; 293..296; 321..324; 326..328; 330..332; 342..346; 358..360; 367..372; 378..381; 396..398; 404..410; 438..440; 443..445; 457..462; 496..498; 504..506; 526..528; 630..633; 658..660; 667..670; 686..690; 694..700; 701..703; 710..716; 733..735; 737..743; 801..804; 806..810; 816..819; 823..826; 830..833; 835..837; 842..846; 853..855; 856..865; 872..879; 882..887; 892..896; 902..904
3D Structure
Electron microscopy (16); NMR spectroscopy (3); X-ray crystallography (23)
Domain & Motif Annotations
Motif
372..375; DECH box
Domain (CC)
The RLR CTR domain controls homooligomerization and interaction with MAVS/IPS1. In the absence of viral infection, the protein is maintained as a monomer in an autoinhibited state with the CARD domains masked through intramolecular interactions with the RLR CTR domain. Upon binding to viral RNA and ubiquitination by RNF135, a conformational change releases the autoinhibition promoting further homooligomerization, interaction of the CARD domains with the adapter protein MAVS/IPS1 and activation of the downstream RIG-I signaling pathway.; DOMAIN: The helicase domain is responsible for dsRNA recognition.; DOMAIN: The 2 CARD domains are responsible for interaction with and signaling through MAVS/IPS1 and for association with the actin cytoskeleton.; DOMAIN: The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
Domain (FT)
1..87; CARD 1; 92..172; CARD 2; 251..430; Helicase ATP-binding; 610..776; Helicase C-terminal; 794..925; RLR CTR
Region
218..925; Interaction with ZC3HAV1; 735..925; Mediates interaction with RNF135
Protein Families
- Helicase family
- RLR subfamily
Sequence Similarities
Belongs to the helicase family. RLR subfamily.
Clinical Relevance
Disease Involvement
Disease variant
Drug Targets
Clinical trial target
Interaction Protein
ENSG00000088888ENSG00000101695ENSG00000121060ENSG00000124535
Interaction Count
4
Interaction Dataset
intact_biogrid