Protein detail

G6B

Megakaryocyte and platelet inhibitory receptor G6b (Protein G6b)

Entry name G6B	UniProt ID O95866	EVMP confidence score 0.38
Supporting publications (n) 3	Transmembrane count 1	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information8

Protein Names

Megakaryocyte and platelet inhibitory receptor G6b (Protein G6b)

Protein Function (3)

Disease related genes
Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins

Transmembrane

143..163; Helical

Transmembrane Count

Ensembl

ENSG00000204420

Entrez Gene Symbol

MPIG6B

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization6

Cell SpecificAdipocytesSingle-Nuclei Brain SpecificfibroblastBlood Cell SpecificgdT-cellBlood Lineage SpecificNK-cellsSecretome LocationSecreted to bloodSecretome FunctionReceptor

Function & Pathway7

Protein Function (3)

Disease related genes
Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins

Cellular Component (5)

Molecular Function (2)

Biological Process (3)

Reactome (3)

Canonical Pathways

M220 Pid caspase pathway

Mediation Categories

Immune mediation

Relations & Evidence35

Ligand-Receptor Signaling (33)

33 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane_phobius	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_sosui	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	Yes	No

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	MPIG6B	O95866	2	PDB	PDB:6r0x

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Size Exclusion Chromatography	Mass spectrometry	1	38343172

Sequence, Structure & Domains10

Sequences

Length

241

Mass

26,163

Sequence

MAVFLQLLPLLLSRAQGNPGASLDGRPGDRVNLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWASSSGTPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPGPTHGSVYPQLLIPLLGAGLVLGLGALGLVWWLHRRLPPQPIRPLPRFAPLVKTEPQRPVKEEEPKIPGDLDQEPSLLYADLDHLALSRPRRLSTADPADASTIYAVVV

Alternative Products

Event=Alternative splicing; Named isoforms=7; Name=B; IsoId=O95866-1; Sequence=Displayed; Name=A; IsoId=O95866-2; Sequence=VSP_014176; Name=C; IsoId=O95866-3; Sequence=VSP_014174; Name=D; IsoId=O95866-4; Sequence=VSP_014172; Name=E; IsoId=O95866-5; Sequence=VSP_014173; Name=F; IsoId=O95866-6; Sequence=VSP_014175; Name=G; IsoId=O95866-7; Sequence=VSP_014177

Alternative Sequence

137..241; GSVYPQLLIPLLGAGLVLGLGALGLVWWLHRRLPPQPIRPLPRFAPLVKTEPQRPVKEEEPKIPGDLDQEPSLLYADLDHLALSRPRRLSTADPADASTIYAVVV -> ALSPPHSSTCENRAPEASKGGRAQDSRGPGPGTEPALCGSGPSSPQQAPPAVHSGPC (in isoform D); 137..180; Missing (in isoform E); 138..180; SVYPQLLIPLLGAGLVLGLGALGLVWWLHRRLPPQPIRPLPRF -> ACPRNRFDHSLDLLCPPHI (in isoform C); 181..241; APLVKTEPQRPVKEEEPKIPGDLDQEPSLLYADLDHLALSRPRRLSTADPADASTIYAVVV -> GETNSTPFSFSYMPTPHPSIPESEPLLGADTLVTFSPSFSSVPPT (in isoform F); 182..241; PLVKTEPQRPVKEEEPKIPGDLDQEPSLLYADLDHLALSRPRRLSTADPADASTIYAVVV -> LSPPHSSTCENRAPEASKGGRAQDSRGPGPGTEPALCGSGPSSPQQAPPAVHSGPC (in isoform A); 182..241; PLVKTEPQRPVKEEEPKIPGDLDQEPSLLYADLDHLALSRPRRLSTADPADASTIYAVVV -> LSPPHSSTCENRAPEASKGGRAQDSRGPGPGTGKGMGMGRG (in isoform G)

3D Structural Models

Turn

99..101

Beta Strand

21..25; 30..33; 67..69; 94..97; 104..106; 111..113; 118..123

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Motif

209..214; ITIM motif; 235..240; ITIM motif

Domain (CC)

Isoform B, displayed in this entry, is the only one of the isoforms to contain both a transmembrane region and 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. The 2 ITIM motifs of isoform B are required for the inhibition of CLEC1B and GP6:FCER1G signaling and platelet activation.

Supporting Publications3

PMID	Title	Abstract
30805606	Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro.	No abstract available
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available
40098346	Toward Identification of Markers for Brain-Derived Extracellular Vesicles in Cerebrospinal Fluid: A Large-Scale, Unbiased Analysis Using Proximity Extension Assays.	No abstract available