Protein detail
PAK4
Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (p21-activated kinase 4) (PAK-4)
Protein symbol PAK4 | UniProt ID | EVMP score 0.38 |
Frequency 1 | Transmembrane count | Protein classification EnzymesPredicted intracellular proteinsRAS pathway related proteins |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (p21-activated kinase 4) (PAK-4)
Protein Class
EnzymesPredicted intracellular proteinsRAS pathway related proteins
Protein Function
- Predicted intracellular proteins
- Kinases:STE Ser/Thr protein kinases
- ENZYME proteins:Transferases
- RAS pathway related proteins
- Enzymes
Ensembl
Entrez Gene Symbol
Gene Description
P21 (RAC1) activated kinase 4
Chromosome
19
Position
39125770-39182816
Frequency
1
EVMP Score
0.38
Fluorescence & Localization
Cell SpecificExtravillous trophoblasts
Function & Pathway
Protein Function
- Predicted intracellular proteins
- Kinases:STE Ser/Thr protein kinases
- ENZYME proteins:Transferases
- RAS pathway related proteins
- Enzymes
Cellular Component
Molecular Function
Biological Process
KEGG
- hsa04012 ErbB signaling pathway
- KEGG:hsa04014 Ras signaling pathway
- KEGG:hsa04360 Axon guidance
- KEGG:hsa04510 Focal adhesion
- KEGG:hsa04517 IgSF CAM signaling
- KEGG:hsa04660 T cell receptor signaling pathway
- KEGG:hsa04810 Regulation of actin cytoskeleton
- KEGG:hsa05170 Human immunodeficiency virus 1 infection
- KEGG:hsa05206 MicroRNAs in cancer
- KEGG:hsa05211 Renal cell carcinoma
Reactome
- R-hsa-428540 activation of rac1
- R-hsa-9013148 cdc42 gtpase cycle
- R-hsa-9675108 nervous system development
- R-hsa-9013149 rac1 gtpase cycle
- R-hsa-9013404 rac2 gtpase cycle
- R-hsa-9013423 rac3 gtpase cycle
- R-hsa-9013408 rhog gtpase cycle
- R-hsa-9013407 rhoh gtpase cycle
- R-hsa-9013409 rhoj gtpase cycle
- R-hsa-9013406 rhoq gtpase cycle
- R-hsa-9013420 rhou gtpase cycle
- R-hsa-9013424 rhov gtpase cycle
- R-hsa-9012999 rho gtpase cycle
- R-hsa-9716542 signaling by rho gtpases miro gtpases and rhobtb3
- R-hsa-376176 signaling by robo receptors
Mediation Categories
Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
9 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| PAK4 | PRKD1 | Q15139 | S | 99 | phosphorylation | Sparser_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper | ProtMapper:23841590ProtMapper:26068882 |
| PAK4 | PRKD1 | Q15139 | S | 474 | phosphorylation | PhosphoSiteSparser_ProtMapperProtMapper | ProtMapper:23841590 |
| PAK4 | PRKD2 | Q9BZL6 | S | 99 | phosphorylation | PhosphoSiteSparser_ProtMapperProtMapper | ProtMapper:23841590 |
| PAK4 | CDK15 | Q96Q40 | S | 291 | phosphorylation | PhosphoSite | |
| PAK4 | PKD1 | P98161 | S | 99 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:23841590ProtMapper:26068882 |
| PAK4 | PKD1 | P98161 | S | 474 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:23841590 |
| PAK4 | PKD2 | Q13563 | S | 99 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:23841590 |
| PAK4 | CD226 | Q15762 | S | 181 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:22238634 |
| PAK4 | CD244 | Q9BZW8 | S | 181 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:22238634 |
Ligand-Receptor Signaling
9 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| transmembrane | transmembrane | Ramilowski_location | No | No | No | No | No |
| transmembrane | transmembrane | OmniPath | No | No | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
Regulatory Interaction Network
16 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| KPCD1 | Q15139 | PAK4 | O96013 | Yes | Yes | No | Sparser_ProtMapperPhosphoSite_norefSIGNORiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:26218748PhosphoSite:11668177SIGNOR:24840177ProtMapper:26068882PhosphoSite:27903866 |
| PAK4 | O96013 | CTNB1 | P35222 | Yes | Yes | No | Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperHINTSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper | HINT:32585314ProtMapper:28007610ProtMapper:23543898ProtMapper:33914026ProtMapper:29207896SIGNOR:22173096ProtMapper:30755582ProtMapper:29330094ProtMapper:26068882ProtMapper:22173096ProtMapper:25335797ProtMapper:25560280ProtMapper:30897078ProtMapper:29399291 |
| PAK4 | O96013 | NF2L2 | Q16236 | Yes | No | Yes | SIGNORSparser_ProtMapperProtMapper | ProtMapper:35108418SIGNOR:35108418 |
| PAK4 | O96013 | FUMH | P07954 | Yes | No | Yes | Sparser_ProtMapperiPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:30683654ProtMapper:30683654PhosphoSite:30683654 |
| PAK4 | O96013 | RAN | P62826 | Yes | Yes | No | PhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper | ProtMapper:22960742SIGNOR:20805321ProtMapper:20805321ProtMapper:23162738 |
| PAK4 | O96013 | SMAD2 | Q15796 | Yes | No | Yes | Sparser_ProtMapperiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperREACH_ProtMapper | SIGNOR:23934187ProtMapper:28007610ProtMapper:23934187ProtMapper:28542136 |
| PAK4 | O96013 | ARHG2 | Q92974 | Yes | No | Yes | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORdbPTM | dbPTM:15827085SIGNOR:23125951 |
| PAK4 | O96013 | ESR1 | P03372 | Yes | Yes | No | Sparser_ProtMapperiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperPhosphoSite_ProtMapper | SIGNOR:26554417ProtMapper:30177834ProtMapper:26554417 |
| PAK4 | O96013 | SYNJ1 | O43426 | Yes | Yes | No | SIGNOR | SIGNOR:22371566 |
| PAK4 | O96013 | ITB5 | P18084 | Yes | Yes | No | Sparser_ProtMapperPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointSIGNORProtMapperREACH_ProtMapperiPTMnetHPRDHINTRLIMS-P_ProtMapperSIGNOR_ProtMapperLit-BM-17PhosphoSitePhosphoSite_ProtMapperSPIKE_LC | HINT:12356872ProtMapper:28007610ProtMapper:20507994HPRD:12356872SIGNOR:20507994PhosphoSite:20507994Lit-BM-17:12356872SPIKE_LC:16189514 |
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Protein Complex Composition
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationSize Exclusion Chromatography | Western blottingMORPH | 1 | 32089743 |
Sequence, Structure & Domains
Sequences
Length
591
Mass
64,072
Sequence
MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR
Alternative Products
Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=O96013-1; Sequence=Displayed; Name=2; IsoId=O96013-2; Sequence=VSP_004892, VSP_004893; Name=3; IsoId=O96013-3; Sequence=VSP_017572; Name=4; IsoId=O96013-4; Sequence=VSP_017573
Alternative Sequence
69..221; Missing (in isoform 3); 120; E -> K (in isoform 2); 121..285; Missing (in isoform 2); 132..221; Missing (in isoform 4)
3D Structural Models
Turn
27..30; 40..42; 46..48; 341..343; 370..372; 514..517; 557..559; 586..588
Helix
37..39; 301..311; 317..319; 354..356; 360..362; 363..369; 403..409; 414..433; 443..445; 459..461; 479..481; 484..487; 495..510; 520..529; 538..540; 543..552; 563..566; 570..574; 578..581; 582..584
Beta Strand
15..26; 31..34; 312..315; 321..329; 331..333; 334..340; 346..353; 381..387; 390..395; 446..448; 454..456; 467..469; 475..477
3D Structure
X-ray crystallography (49)
Domain & Motif Annotations
Compositional Bias
149..164; Basic and acidic residues; 191..202; Low complexity; 242..260; Low complexity; 271..290; Pro residues; 292..301; Basic and acidic residues
Domain (FT)
11..24; CRIB; 321..572; Protein kinase
Region
25..320; Linker; 95..301; Disordered; 298..323; GEF-interaction domain (GID)
Protein Families
- Protein kinase superfamily
- STE Ser/Thr protein kinase family
- STE20 subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Clinical Relevance
Related Diseases
Biomarker
Phase 1
Drugs
Interaction Protein
ENSG00000070831ENSG00000108953ENSG00000128245ENSG00000134308ENSG00000164924ENSG00000166913ENSG00000170027ENSG00000175793
Interaction Count
8
Interaction Dataset
intact_biogridintact_biogrid_opencellbiogrid_opencell