EV MemProt Atlas

Basic Information

Protein Names

Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (p21-activated kinase 4) (PAK-4)

Protein Class

EnzymesPredicted intracellular proteinsRAS pathway related proteins

Protein Function

Predicted intracellular proteins
Kinases:STE Ser/Thr protein kinases
ENZYME proteins:Transferases
RAS pathway related proteins
Enzymes

Ensembl

ENSG00000130669

Entrez Gene Symbol

PAK4

Gene Description

P21 (RAC1) activated kinase 4

Chromosome

19

Position

39125770-39182816

Frequency

1

EVMP Score

0.38

Fluorescence & Localization

Cell SpecificExtravillous trophoblasts

Function & Pathway

Protein Function

Predicted intracellular proteins
Kinases:STE Ser/Thr protein kinases
ENZYME proteins:Transferases
RAS pathway related proteins
Enzymes

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Mediation Categories

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PAK4	PRKD1	Q15139	S	99	phosphorylation	Sparser_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	ProtMapper:23841590 ProtMapper:26068882
PAK4	PRKD1	Q15139	S	474	phosphorylation	PhosphoSiteSparser_ProtMapperProtMapper	ProtMapper:23841590
PAK4	PRKD2	Q9BZL6	S	99	phosphorylation	PhosphoSiteSparser_ProtMapperProtMapper	ProtMapper:23841590
PAK4	CDK15	Q96Q40	S	291	phosphorylation	PhosphoSite
PAK4	PKD1	P98161	S	99	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:23841590 ProtMapper:26068882
PAK4	PKD1	P98161	S	474	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:23841590
PAK4	PKD2	Q13563	S	99	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:23841590
PAK4	CD226	Q15762	S	181	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:22238634
PAK4	CD244	Q9BZW8	S	181	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:22238634

Ligand-Receptor Signaling

9 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

16 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PAK4	O96013	SNAI2	O43623	Yes	Yes	No	PhosphoSite_norefSIGNORProtMapperRLIMS-P_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:29849120 PhosphoSite:28288135 PhosphoSite:29849120 SIGNOR:29849120
PAK4	O96013	MZF1	P28698	Yes	Yes	No	SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:30622337 SIGNOR:30622337
KPCD3	O94806	PAK4	O96013	Yes	Yes	No	SIGNOR	SIGNOR:23841590
PAK4	O96013	PACN1	Q9BY11	Yes	Yes	No	SIGNOR_ProtMapperSIGNORProtMapper	SIGNOR:22371566 ProtMapper:22371566
PAK4	O96013	WASL	O00401	Yes	No	No	PhosphoSite_norefProtMapperIntActREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:21898535 IntAct:29100370 PhosphoSite:29100370 ProtMapper:29100370
PAK4	O96013	RUNX1	Q01196	Yes	No	No	Sparser_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	ProtMapper:32549768 PhosphoSite:32549768

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Protein Complex Composition

14 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARHGAP31 CDC42 FHOD1 NCF1 PAK4 PAK5 PLEKHG2 RAC1 TBC1D3F	A6NER0 O96013 P14598 P60953 P63000 Q2M1Z3 Q9H7P9 Q9P286 Q9Y613	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7542
GAK HSPA1B HSPA8 MAP3K5 MAP3K6 PAK4 PGAM5 PPP5C UBC YWHAB YWHAG YWHAH YWHAQ YWHAZ	O14976 O95382 O96013 P0CG48 P0DMV9 P11142 P27348 P31946 P53041 P61981 P63104 Q04917 Q96HS1 Q99683	1:1:1:1:2:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5371
CDC42 MFN2 PAK4 PRKN	O60260 O95140 O96013 P60953	1:1:1:1	NetworkBlastCompleat	Compleat:HC9161
PAK4	O96013	2	PDB	PDB:4l67PDB:2bvaPDB:4figPDB:4fiePDB:4fifPDB:4fii
DAPP1 EXO1 GRK1 HSPA1B PAK4 PI4KB RCVRN SFN YWHAB YWHAG YWHAQ YWHAZ	O96013 P0DMV9 P27348 P31946 P31947 P35243 P61981 P63104 Q15835 Q9UBF8 Q9UN19 Q9UQ84	1:2:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9265
ARHGAP27 ARHGAP31 CDC42 NCF1 NEB PAK4 PAK5 PLEKHG2 RAC1	O96013 P14598 P20929 P60953 P63000 Q2M1Z3 Q6ZUM4 Q9H7P9 Q9P286	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6125
CDC42 DIRAS2 FHL1 FHL2 NCF1 NEB PAK4 PLEKHG2	O96013 P14598 P20929 P60953 Q13642 Q14192 Q96HU8 Q9H7P9	1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7294
ARHGAP27 ARHGAP31 CDC42 LIMK1 NCF1 PAK4 PLEKHG2 RAC1 RAP1A RAP1GDS1	O96013 P14598 P52306 P53667 P60953 P62834 P63000 Q2M1Z3 Q6ZUM4 Q9H7P9	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9188
LIMK1 PAK4	O96013 P53667	1:1	PDB	PDB:6wly
ARHGAP31 CDC42 LIMK1 MAP3K15 PAK2 PAK4 PLEKHG2 RAC1 RAP1B SH3PXD2A	O96013 P53667 P60953 P61224 P63000 Q13177 Q2M1Z3 Q5TCZ1 Q6ZN16 Q9H7P9	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9051

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Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Western blottingMORPH	1	32089743

Sequence, Structure & Domains

Sequences

Length

591

Mass

64,072

Sequence

MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR

Alternative Products

Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=O96013-1; Sequence=Displayed; Name=2; IsoId=O96013-2; Sequence=VSP_004892, VSP_004893; Name=3; IsoId=O96013-3; Sequence=VSP_017572; Name=4; IsoId=O96013-4; Sequence=VSP_017573

Alternative Sequence

69..221; Missing (in isoform 3); 120; E -> K (in isoform 2); 121..285; Missing (in isoform 2); 132..221; Missing (in isoform 4)

3D Structural Models

Turn

27..30; 40..42; 46..48; 341..343; 370..372; 514..517; 557..559; 586..588

Helix

37..39; 301..311; 317..319; 354..356; 360..362; 363..369; 403..409; 414..433; 443..445; 459..461; 479..481; 484..487; 495..510; 520..529; 538..540; 543..552; 563..566; 570..574; 578..581; 582..584

Beta Strand

15..26; 31..34; 312..315; 321..329; 331..333; 334..340; 346..353; 381..387; 390..395; 446..448; 454..456; 467..469; 475..477

3D Structure

X-ray crystallography (49)

Domain & Motif Annotations

Compositional Bias

149..164; Basic and acidic residues; 191..202; Low complexity; 242..260; Low complexity; 271..290; Pro residues; 292..301; Basic and acidic residues

Domain (FT)

11..24; CRIB; 321..572; Protein kinase

Region

25..320; Linker; 95..301; Disordered; 298..323; GEF-interaction domain (GID)

Protein Families

Protein kinase superfamily
STE Ser/Thr protein kinase family
STE20 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Clinical Relevance

Related Diseases

Malignant haematopoietic neoplasm Solid tumour/cancer

Biomarker

Phase 1

Antibody

CAB025747 CAB044670 HPA070175 HPA072220

Interaction Protein

ENSG00000070831ENSG00000108953ENSG00000128245ENSG00000134308ENSG00000164924ENSG00000166913ENSG00000170027ENSG00000175793

Interaction Count

8

Interaction Dataset

intact_biogridintact_biogrid_opencellbiogrid_opencell

Protein symbol PAK4	UniProt ID O96013	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification EnzymesPredicted intracellular proteinsRAS pathway related proteins