Protein detail

CRYAB

Alpha-crystallin B chain (Alpha(B)-crystallin) (Heat shock protein beta-5) (HspB5) (Heat shock protein family B member 5) (Renal carcinoma antigen NY-REN-27) (Rosenthal fiber component)

Entry name CRYAB	UniProt ID P02511	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Cancer-related genesDisease related genesHuman disease related genesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Alpha-crystallin B chain (Alpha(B)-crystallin) (Heat shock protein beta-5) (HspB5) (Heat shock protein family B member 5) (Renal carcinoma antigen NY-REN-27) (Rosenthal fiber component)

Protein Class (7)

Cancer-related genesDisease related genesHuman disease related genesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function (8)

Predicted intracellular proteins
Potential drug targets
Human disease related genes:Musculoskeletal diseases:Muscular diseases
Cancer-related genes:Candidate cancer biomarkers
Transporters:Accessory Factors Involved in Transport
Disease related genes
Human disease related genes:Cardiovascular diseases:Cardiac diseases
Human disease related genes:Nervous system diseases:Eye disease

Ensembl

ENSG00000109846

Entrez Gene Symbol

CRYAB

Gene Synonym (2)

CRYA2HSPB5

Gene Description

Crystallin alpha B

Chromosome

Position

111908564-111923722

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization5

Tissue Specificbone marrowCell SpecificPlateletsBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway8

Protein Function (8)

Predicted intracellular proteins
Potential drug targets
Human disease related genes:Musculoskeletal diseases:Muscular diseases
Cancer-related genes:Candidate cancer biomarkers
Transporters:Accessory Factors Involved in Transport
Disease related genes
Human disease related genes:Cardiovascular diseases:Cardiac diseases
Human disease related genes:Nervous system diseases:Eye disease

Cellular Component (17)

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Molecular Function (10)

Biological Process (3)

KEGG (2)

Reactome (3)

Canonical Pathways (2)

M11736 Sa mmp cytokine connection
M63 Pid avb3 opn pathway

Mediation Categories (3)

Fusion and delivery mediationImmune mediationMetabolism mediation

Relations & Evidence33

Enzyme-Mediated Modification (10)

10 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
CRYAB	MAPK14	Q16539	S	59	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
CRYAB	CAT	P04040	S	59	phosphorylation	Sparser_ProtMapperProtMapper	ProtMapper:21751351
CRYAB	GKN2	Q86XP6	S	59	phosphorylation	Sparser_ProtMapperProtMapper	ProtMapper:25332170
CRYAB	CDK2	P24941	S	19	phosphorylation	KEA	KEA:17570479
CRYAB	CDK2	P24941	S	45	phosphorylation	KEA	KEA:17570479
CRYAB	MAPK1	P28482	S	45	phosphorylation	KEA	KEA:16928191
CRYAB	MAPK3	P27361	S	45	phosphorylation	KEA	KEA:16928191
CRYAB	MAPKAPK2	P49137	S	59	phosphorylation	KEA	KEA:16928191
CRYAB	PPP1CA	P62136	S	59	phosphorylation	KEA	KEA:16928191
CRYAB	PRKG1	Q13976	S	59	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (11)

11 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	GO_Intercell	Yes	No	Yes	No	No
ecm	ecm	OmniPath	Yes	No	Yes	No	No
extracellular	extracellular	OmniPath	No	No	Yes	No	No
intracellular	intracellular	LOCATE	No	No	Yes	No	No
intracellular	intracellular	ComPPI	No	No	Yes	No	No
intracellular	intracellular	GO_Intercell	No	No	Yes	No	No
intracellular	intracellular	UniProt_location	No	No	Yes	No	No
intracellular	intracellular	OmniPath	No	No	Yes	No	No
secreted	secreted	UniProt_keyword	No	No	Yes	No	No
secreted	secreted	UniProt_location	No	No	Yes	No	No

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Regulatory Interaction Network (4)

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
CRYAB	P02511	CRGD	P07320	Yes	Yes	No	HINTLit-BM-17SIGNORBioGRID	HINT:22655036 Lit-BM-17:16274233 Lit-BM-17:21173272 SIGNOR:20621668 HINT:21173272 BioGRID:21173272 Lit-BM-17:12601044 HINT:16274233 HINT:12601044 Lit-BM-17:22655036
CRYAB	P02511	CRGC	P07315	Yes	Yes	No	SIGNORHPRDHINTIntActSPIKE_LCLit-BM-17	IntAct:12601044 SIGNOR:20621668 SPIKE_LC:11700327 HPRD:11700327 Lit-BM-17:11700327 HINT:11700327 Lit-BM-17:12601044 HINT:12601044 IntAct:11700327
CRYAB	P02511	CRYGS	P22914	Yes	Yes	No	DIPSIGNOR	SIGNOR:20621668 DIP:24183572
MK14	Q16539	CRYAB	P02511	Yes	No	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:18061612 PhosphoSite:26620801 PhosphoSite:9368070 PhosphoSite:20005263 PhosphoSite:16500585 PhosphoSite:16129694 PhosphoSite:19272359 PhosphoSite:24023879 PhosphoSite:11096101 PhosphoSite:16928191 PhosphoSite:23049853 PhosphoSite:10706390 PhosphoSite:11158243 PhosphoSite:12468532 PhosphoSite:8175657 PhosphoSite:15511225 PhosphoSite:27641668 PhosphoSite:12060738

Protein Complex Composition (7)

7 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
SCF-FBX4-aLpha-B Crystallin	CRYAB CUL1 FBXO4 SKP1	P02511 P63208 Q13616 Q9UKT5	0:0:0:0	SPIKE
	CRYAB OGA	O60502 P02511	2:2	PDB	PDB:5vvv
	ACO2 ACTB ACTN2 CKM CRYAB CS GCN1 H1-5 MYH2 NTPCR TNNI3 TNNT2 TPM1 ZFP36	O75390 P02511 P06732 P09493 P16401 P19429 P26651 P35609 P45379 P60709 Q92616 Q99798 Q9BSD7 Q9UKX2	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5833
	BAG3 CRYAB FOS GTF3C3 HCFC1 HSPA1B HSPA8 HSPB2 HSPB8 NLK WIPI2	O95817 P01100 P02511 P0DMV9 P11142 P51610 Q16082 Q9UBE8 Q9UJY1 Q9Y4P8 Q9Y5Q9	1:1:1:2:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9146
	CRYAB	P02511	2	PDB	PDB:2klrPDB:2ygdPDB:3sgpPDB:3sgrPDB:7rojPDB:3j07PDB:2n0kPDB:3sgmPDB:2y1zPDB:6bp9PDB:2y22PDB:2wj7PDB:4m5tPDB:4m5sPDB:3sgn
	CRYAB HSPB2 HSPB8	P02511 Q16082 Q9UJY1	1:1:1	CompleatCFinder	Compleat:HC6283
	CRYAB NYNRIN	P02511 Q9P2P1	0:0	hu.MAPhu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	31382537

Sequence, Structure & Domains11

Sequences

Length

175

Mass

20,159

Sequence

MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK

3D Structural Models

Turn

125..127

Helix

86..88; 130..132

Beta Strand

68..70; 72..80; 82..84; 89..94; 97..109; 112..123; 134..137; 141..148; 157..159; 161..163

3D Structure

Electron microscopy (2); NMR spectroscopy (3); X-ray crystallography (15)

Domain & Motif Annotations

Domain (FT)

56..164; sHSP

Region

146..175; Disordered

Protein Families

Small heat shock protein (HSP20) family

Sequence Similarities

Belongs to the small heat shock protein (HSP20) family.

Clinical Relevance5

Disease Involvement (5)

Cancer-related genesCardiomyopathyCataractDisease variantMyofibrillar myopathy

Antibody (3)

CAB002053 CAB040560 HPA057100

Interaction Protein (9)

ENSG00000106211ENSG00000108255ENSG00000142192ENSG00000152137ENSG00000160202ENSG00000163254ENSG00000168036ENSG00000170276ENSG00000244752

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
32795414	Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.	Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers.