Protein detail
RAF1
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Entry name RAF1 | UniProt ID | EVMP confidence score 0.50 |
Supporting publications (n) 1 | Transmembrane count | Protein classification Cancer-related genesDisease related genesEnzymesFDA approved drug targetsHuman disease related genesPredicted intracellular proteinsRAS pathway related proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Protein Class (7)
Cancer-related genesDisease related genesEnzymesFDA approved drug targetsHuman disease related genesPredicted intracellular proteinsRAS pathway related proteins
Protein Function (10)
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- RAS pathway related proteins
- Enzymes
- Cancer-related genes:Candidate cancer biomarkers
- Kinases:TKL Ser/Thr protein kinases
- Human disease related genes:Congenital malformations:Other congenital malformations
- Disease related genes
- Human disease related genes:Cardiovascular diseases:Cardiac diseases
- FDA approved drug targets:Small molecule drugs
Ensembl
Entrez Gene Symbol
Gene Synonym (3)
c-RafCRAFRaf-1
Gene Description
Raf-1 proto-oncogene, serine/threonine kinase
Chromosome
3
Position
12582101-12664201
Supporting publications (n)
1
EVMP confidence score
0.50
Fluorescence & Localization2
Cell SpecificEpicardial cells
Function & Pathway7
Protein Function (10)
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- RAS pathway related proteins
- Enzymes
- Cancer-related genes:Candidate cancer biomarkers
- Kinases:TKL Ser/Thr protein kinases
- Human disease related genes:Congenital malformations:Other congenital malformations
- Disease related genes
- Human disease related genes:Cardiovascular diseases:Cardiac diseases
- FDA approved drug targets:Small molecule drugs
Cellular Component (8)
Molecular Function (10)
- GO:0004672 protein kinase activity
- GO:0004674 protein serine/threonine kinase activity
- GO:0004709 MAP kinase kinase kinase activity
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0019899 enzyme binding
- GO:0042802 identical protein binding
- GO:0044024 histone H2AS1 kinase activity
- GO:0046872 metal ion binding
- GO:0106310 protein serine kinase activity
Biological Process (3)
KEGG (84)
- hsa01521 EGFR tyrosine kinase inhibitor resistance
- KEGG:hsa01522 Endocrine resistance
- KEGG:hsa04010 MAPK signaling pathway
- KEGG:hsa04012 ErbB signaling pathway
- KEGG:hsa04014 Ras signaling pathway
- KEGG:hsa04015 Rap1 signaling pathway
- KEGG:hsa04022 cGMP-PKG signaling pathway
- KEGG:hsa04024 cAMP signaling pathway
- KEGG:hsa04062 Chemokine signaling pathway
- KEGG:hsa04068 FoxO signaling pathway
Page 1 of 9
Reactome (26)
- R-hsa-1280218 adaptive immune system
- R-hsa-5621575 cd209 dc sign signaling
- R-hsa-1280215 cytokine signaling in immune system
- R-hsa-5621481 c type lectin receptors clrs
- R-hsa-5663202 diseases of signal transduction by growth factor receptors and second messengers
- R-hsa-430116 gp1b ix v activation signalling
- R-hsa-109582 hemostasis
- R-hsa-9732724 ifng signaling activates mapks
- R-hsa-168249 innate immune system
- R-hsa-877300 interferon gamma signaling
Page 1 of 3
Mediation Categories (5)
Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation
Relations & Evidence285
Enzyme-Mediated Modification (201)
201 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| RAF1 | PAK1 | Q13153 | S | 233 | phosphorylation | PhosphoNetworks | |
| RAF1 | PAK1 | Q13153 | T | 258 | phosphorylation | PhosphoNetworks | |
| RAF1 | PAK1 | Q13153 | S | 259 | phosphorylation | Sparser_ProtMapperProtMapper | ProtMapper:25843685 |
| RAF1 | SRC | P12931 | Y | 341 | phosphorylation | BEL-Large-Corpus_ProtMapperSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperReactome_ProtMapperdbPTMKEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:29063113ProtMapper:12551923KEA:10205168KEA:17097642ProtMapper:22592532KEA:15258589ProtMapper:16170185ProtMapper:16630891KEA:8876196SIGNOR:12551923dbPTM:-phosphoELM:10205168 |
| RAF1 | SRC | P12931 | Y | 340 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperdbPTMKEASIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:12952943ProtMapper:12551923ProtMapper:7692235KEA:17097642KEA:15710605dbPTM:-ProtMapper:29063113ProtMapper:10998357SIGNOR:12551923SIGNOR:10998357SIGNOR:7692235 |
| RAF1 | SRC | P12931 | S | 338 | phosphorylation | Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperHPRDReactome_ProtMapperKEA | HPRD:17097642KEA:17097642ProtMapper:11376654 |
| RAF1 | SRC | P12931 | S | 339 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA | KEA:17097642 |
| RAF1 | SRC | P12931 | T | 491 | phosphorylation | Reactome_ProtMapperProtMapper | |
| RAF1 | SRC | P12931 | S | 494 | phosphorylation | Reactome_ProtMapperProtMapper | |
| RAF1 | MAPK1 | P28482 | S | 43 | phosphorylation | SIGNORProtMapperdbPTMReactome_ProtMapperSIGNOR_ProtMapper | SIGNOR:15664191dbPTM:8349614ProtMapper:15664191dbPTM:10801873dbPTM:18691976 |
Ligand-Receptor Signaling (9)
9 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
Regulatory Interaction Network (52)
52 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| RAF1 | P04049 | KLF10 | Q13118 | Yes | No | Yes | Sparser_ProtMapperiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:23994618SIGNOR:23994618ProtMapper:23994618 |
| RAF1 | P04049 | MYC | P01106 | Yes | No | No | PhosphoSite_MIMPMIMPPhosphoPointProtMapperHPRDHINTdbPTMBioGRIDPhosphoSitePhosphoSite_ProtMapper | dbPTM:9315742HPRD:9315742BioGRID:9315742HINT:9315742PhosphoSite:9315742 |
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Protein Complex Composition (22)
22 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| RAF1SFN | P04049P31947 | 2:2 | PDB | PDB:3iquPDB:8atrPDB:9ew1PDB:8av0PDB:8q5cPDB:3iqvPDB:9ew7PDB:8a68PDB:8atsPDB:8a6fPDB:9ew3PDB:9ew5PDB:3iqjPDB:9ew4PDB:8a6hPDB:8s42PDB:4ieaPDB:3o8i | ||
| RAF1RAP1A | P04049P62834 | 1:1 | PDB | PDB:3kucPDB:1c1yPDB:1gua | ||
| RAF1YWHAZ | P04049P63104 | 2:2 | PDB | PDB:4ihlPDB:4fj3PDB:3cu8PDB:3nkx | ||
| DNAJB2ZFTRAF1 | P0DTL6P25686 | 0:0 | hu.MAP | |||
| UBE2KZFTRAF1 | P0DTL6P61086 | 0:0 | hu.MAP2 | |||
| CCDC121CFAP263LTBRTRAF1 | P36941Q13077Q6ZUS5Q9H0I3 | 0:0:0:0 | hu.MAP | |||
| CCDC121CFAP263HDAC5LTBRTRAF1 | P36941Q13077Q6ZUS5Q9H0I3Q9UQL6 | 0:0:0:0:0 | hu.MAP | |||
| HTRA4LAMC3OTUD4SNX29TANKTRAF1TRAF2 | P83105Q01804Q12933Q13077Q8TEQ0Q92844Q9Y6N6 | 0:0:0:0:0:0:0 | hu.MAP2 | |||
| OTUD4SNX29TANKTRAF1TRAF2 | Q01804Q12933Q13077Q8TEQ0Q92844 | 0:0:0:0:0 | hu.MAP2 | |||
| BIRC3TRAF1TRAF2 | Q12933Q13077Q13489 | 2:1:1 | PDB | PDB:3m0d |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Mass spectrometry [LTQ-FT Ultra]Mass spectrometry | 1 | 29242380 |
Sequence, Structure & Domains15
Sequences
Length
648
Mass
73,052
Sequence
MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=6C; IsoId=P04049-1; Sequence=Displayed; Name=2; Synonyms=1A; IsoId=P04049-2; Sequence=VSP_034649
Alternative Sequence
278; E -> ENNNLSASPRAWSRRFCLRGR (in isoform 2)
3D Structural Models
Turn
63..65; 153..155; 166..168; 434..437; 478..480; 547..550; 593..595; 612..614
Helix
78..88; 93..95; 118..121; 174..179; 384..397; 442..461; 471..473; 514..518; 527..542; 554..563; 580..588; 599..611
Beta Strand
57..61; 67..71; 96..100; 103..107; 110..112; 125..130; 132..134; 135..137; 141..145; 160..165; 170..172; 182..185; 350..353; 363..376; 408..412; 414..416; 418..422; 429..431; 481..484; 510..512; 520..522
3D Structure
Electron microscopy (9); NMR spectroscopy (7); X-ray crystallography (43)
Domain & Motif Annotations
Compositional Bias
239..271; Polar residues; 275..285; Basic and acidic residues; 286..301; Low complexity
Zinc Finger
138..184; Phorbol-ester/DAG-type
Domain (FT)
56..131; RBD; 349..609; Protein kinase
Region
220..334; Disordered; 331..349; Interaction with PEBP1/RKIP
Protein Families (3)
- Protein kinase superfamily
- TKL Ser/Thr protein kinase family
- RAF subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Clinical Relevance7
Disease Involvement (6)
Cancer-related genesCardiomyopathyDeafnessDisease variantFDA approved drug targetsProto-oncogene
Drug Targets
FDA approved drug targets
Drugs (26)
SORAFENIBTOVORAFENIBREGORAFENIBC-RAF ANTISENSE OLIGONUCLEOTIDE ISIS 5132ENCORAFENIBXL281COBIMETINIBCARBOPLATINSEMAPIMODTRAMETINIB DIMETHYL SULFOXIDERG-7376LGX-806NULLXL-281BELVARAFENIBPACLITAXELLXH254ARQ-736CHIR-265U0126GEMCITABINEB-RAF/VEGFR-2 INHIBITOR RAF265DOCETAXEL ANHYDROUSLY-3009120CISPLATINICO-007
Interaction Protein (32)
ENSG00000044574ENSG00000078061ENSG00000080824ENSG00000089220ENSG00000095002ENSG00000096060ENSG00000096384ENSG00000103266ENSG00000104375ENSG00000105197ENSG00000105401ENSG00000107262ENSG00000108953ENSG00000116473ENSG00000126934ENSG00000128245ENSG00000128731ENSG00000133703ENSG00000134020ENSG00000134308ENSG00000142208ENSG00000146648ENSG00000157764ENSG00000163468ENSG00000164902ENSG00000164924ENSG00000166913ENSG00000169032ENSG00000170027ENSG00000174775ENSG00000175793ENSG00000213281
Interaction Count
32
Interaction Dataset (3)
intact_biogridbiogrid_opencellintact_biogrid_opencell
Supporting Publications1
| PMID | Title | Abstract |
|---|---|---|
| 38731868 | The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer. | No abstract available |