Protein detail
ANXA1
Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35) [Cleaved into: Annexin Ac2-26]
Entry name ANXA1 | UniProt ID | EVMP confidence score 0.63 |
Supporting publications (n) 10 | Transmembrane count | Protein classification Cancer-related genesFDA approved drug targetsPlasma proteinsPredicted intracellular proteinsPredicted secreted proteinsTransporters |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35) [Cleaved into: Annexin Ac2-26]
Protein Class (6)
Cancer-related genesFDA approved drug targetsPlasma proteinsPredicted intracellular proteinsPredicted secreted proteinsTransporters
Protein Function (5)
- Predicted intracellular proteins
- Transporters:Transporter channels and pores
- Cancer-related genes:Candidate cancer biomarkers
- Predicted secreted proteins
- FDA approved drug targets:Small molecule drugs
Ensembl
Entrez Gene Symbol
Gene Synonym (2)
ANX1LPC1
Gene Description
Annexin A1
Chromosome
9
Position
73151865-73170393
Supporting publications (n)
10
EVMP confidence score
0.63
Fluorescence & Localization4
Tissue SpecificbrainCell SpecificAlveolar cells type 1Single-Nuclei Brain Specificdeep-layer near-projecting
Function & Pathway6
Protein Function (5)
- Predicted intracellular proteins
- Transporters:Transporter channels and pores
- Cancer-related genes:Candidate cancer biomarkers
- Predicted secreted proteins
- FDA approved drug targets:Small molecule drugs
Cellular Component (24)
- GO:0001533 cornified envelope
- GO:0001891 phagocytic cup
- GO:0005576 extracellular region
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0005654 nucleoplasm
- GO:0005737 cytoplasm
- GO:0005768 endosome
- GO:0005829 cytosol
- GO:0005884 actin filament
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Molecular Function (10)
- GO:0001786 phosphatidylserine binding
- GO:0005102 signaling receptor binding
- GO:0005509 calcium ion binding
- GO:0005515 protein binding
- GO:0005543 phospholipid binding
- GO:0005544 calcium-dependent phospholipid binding
- GO:0008289 lipid binding
- GO:0019834 phospholipase A2 inhibitor activity
- GO:0048306 calcium-dependent protein binding
- GO:0098641 cadherin binding involved in cell-cell adhesion
Biological Process (3)
Reactome (15)
- R-hsa-373076 class a 1 rhodopsin like receptors
- R-hsa-1280215 cytokine signaling in immune system
- R-hsa-9734767 developmental cell lineages
- R-hsa-9820448 developmental cell lineages of the exocrine pancreas
- R-hsa-9925563 developmental lineage of pancreatic ductal cells
- R-hsa-444473 formyl peptide receptors bind formyl peptides and many other ligands
- R-hsa-500792 gpcr ligand binding
- R-hsa-418594 g alpha i signalling events
- R-hsa-416476 g alpha q signalling events
- R-hsa-6785807 interleukin 4 and interleukin 13 signaling
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Mediation Categories (5)
Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation
Relations & Evidence97
Enzyme-Mediated Modification (29)
29 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| ANXA1 | ABL1 | P00519 | Y | 21 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperKEAphosphoELMPhosphoSitePhosphoSite_ProtMapper | KEA:2457390phosphoELM:2457390 |
| ANXA1 | EGFR | P00533 | Y | 21 | phosphorylation | Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperdbPTMKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | KEA:2457390SIGNOR:24103589ProtMapper:24103589dbPTM:17081983dbPTM:2457390phosphoELM:2457390ProtMapper:22792315ProtMapper:9223619KEA:3020049 |
| ANXA1 | SRC | P12931 | Y | 21 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | KEA:2457390SIGNOR:24103589phosphoELM:2457390ProtMapper:24103589 |
| ANXA1 | SRC | P12931 | T | 216 | phosphorylation | SIGNOR_ProtMapperSIGNORProtMapper | SIGNOR:24103589ProtMapper:24103589 |
| ANXA1 | PRKCA | P17252 | S | 27 | phosphorylation | BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | KEA:2457390SIGNOR:24103589ProtMapper:15212693ProtMapper:24103589phosphoELM:2457390KEA:88309771 |
| ANXA1 | PRKCA | P17252 | S | 28 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| ANXA1 | PRKCA | P17252 | T | 24 | phosphorylation | MIMPphosphoELM_MIMP | |
| ANXA1 | PRKCG | P05129 | S | 27 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapper | SIGNOR:24103589ProtMapper:24103589 |
| ANXA1 | PRKCG | P05129 | S | 28 | phosphorylation | MIMPphosphoELM_MIMPPhosphoSite_MIMP | |
| ANXA1 | PRKCG | P05129 | T | 24 | phosphorylation | MIMPphosphoELM_MIMP |
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Ligand-Receptor Signaling (45)
45 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| cell_surface_ligand | cell_surface_ligand | Baccin2019 | Yes | No | Yes | No | No |
| cell_surface_ligand | cell_surface_ligand | CellPhoneDB | Yes | No | Yes | No | No |
| cell_surface_ligand | cell_surface_ligand | OmniPath | Yes | No | Yes | No | No |
| cell_adhesion | cell_adhesion | Cellinker | Yes | Yes | Yes | No | No |
| adhesion | adhesion | OmniPath | Yes | Yes | Yes | No | No |
| cell_adhesion | cell_adhesion | OmniPath | Yes | Yes | Yes | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | Yes | No | No |
| plasma_membrane | plasma_membrane | Cellinker | No | No | Yes | No | No |
| basolateral_cell_membrane | plasma_membrane | UniProt_location | No | No | Yes | No | No |
| apical_cell_membrane | plasma_membrane | UniProt_location | No | No | Yes | No | No |
Regulatory Interaction Network (11)
11 records.
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Protein Complex Composition (11)
11 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| ANXA11C19orf47CAAP1CCDC9BCHTOPDDX39BDNAI1FYTTD1NCBP3POLDIP3RBM33SARNPTHOC1THOC2THOC3THOC5THOC6THOC7ZC3H11A | O75152P50995P82979Q13769Q13838Q53F19Q6I9Y2Q6ZUT6Q86W42Q8N9M1Q8NI27Q96EV2Q96FV9Q96J01Q96QD9Q9BY77Q9H8G2Q9UI46Q9Y3Y2 | 0:0:0:0:0:0:0:0:0:0:0:0:0:0:0:0:0:0:0 | hu.MAP2 | |||
| ANXA11COL3A1 | P02461P50995 | 0:0 | hu.MAP | |||
| ANXA1FPR2 | P04083P25090 | 0:0 | Cellinker | |||
| ANXA1CRNNSPRR3 | P04083Q9UBC9Q9UBG3 | 0:0:0 | hu.MAP | |||
| ANXA11ATP5MECCDC68RPS21SLC25A5 | P05141P50995P56385P63220Q9H2F9 | 0:0:0:0:0 | Havugimana2012 | Havugimana2012:C_446 | ||
| ANXA11ANXA7 | P20073P50995 | 0:0 | hu.MAP2 | |||
| ANXA11ANXA7MOB1A | P20073P50995Q9H8S9 | 0:0:0 | hu.MAP2 | |||
| ANXA13 | P27216 | 3 | PDB | PDB:6b3i | ||
| ANXA11TARDBP | P50995Q13148 | 6:6 | PDB | PDB:9forPDB:9fof | ||
| ANXA11DNAI1THOC6 | P50995Q86W42Q9UI46 | 0:0:0 | hu.MAP2 |
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Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity Capture | Mass spectrometrySmall R sequencing (Illumi HiSeq 2000 (Solexa) | 16 | 38590132379267563606464738113368373224753330447841068253335925003801459530915084329169863614683438207106390017003698231237670049 |
Sequence, Structure & Domains10
Sequences
Length
346
Mass
38,714
Sequence
MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
3D Structural Models
Helix
3..10; 16..18; 46..55; 65..71; 77..87; 94..99; 106..109; 110..112
Beta Strand
62..64
3D Structure
NMR spectroscopy (2); X-ray crystallography (2)
Domain & Motif Annotations
Repeat
42..113; Annexin 1; 114..185; Annexin 2; 197..269; Annexin 3; 273..344; Annexin 4
Domain (CC)
The full-length protein can bind eight Ca(2+) ions via the annexin repeats. Calcium binding causes a major conformation change that modifies dimer contacts and leads to surface exposure of the N-terminal phosphorylation sites; in the absence of Ca(2+), these sites are buried in the interior of the protein core. The N-terminal region becomes disordered in response to calcium-binding.
Protein Families
Annexin family
Sequence Similarities
Belongs to the annexin family.
Clinical Relevance7
Disease Involvement (2)
Cancer-related genesFDA approved drug targets
Drug Targets (3)
FDA approved drug targetsLiterature-reported targetClinical trial target
Drugs (44)
HALOBETASOLSTAUROSPORINEDEXAMETHASONEDNPAFLUMETHASONE PIVALATEHYDROCORTAMATEDESOXIMETASONERFRP-3PANCREATIC POLYPEPTIDECLOBETASOL1DMEPREDNISONEPREDNISOLONETHERAPEUTIC GLUCOCORTICOIDBIBP3226DESONIDEPREDNICARBATERIMEXOLONECOMPOUND 46 [PMID: 25268943]PHORBOL 12-MYRISTATE 13-ACETATEEFWSLAAPQRF-NH2METHYLPREDNISOLONEMOMETASONE[3H]EYFST 638CLOCORTOLONE[125I]NPFFDIFLORASONE DIACETATE[125I]EYFTHERAPEUTIC CORTICOSTEROIDAC263093RITUXIMABY-RFRP-3BETAMETHASONETRIAMCINOLONETETRADECANOYLPHORBOL ACETATEBECLOMETHASONEHYDROCORTISONE BUTYRATE4-PHENYLENEDIAMINELOTEPREDNOLRF9NEUROPEPTIDE FFALCLOMETASONEAMCINONIDE
Interaction Protein (3)
ENSG00000137275ENSG00000146648ENSG00000269335
Interaction Count
3
Interaction Dataset
intact_biogrid
Supporting Publications10
| PMID | Title | Abstract |
|---|---|---|
| 25664854 | Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. | In this work, we demonstrate that endogenous annexin A1 (ANXA1) is released as a component of extracellular vesicles (EVs) derived from intestinal epithelial cells, and these ANXA1-containing EVs activate wound repair circuits. |
| 30142511 | Annexin A1 regulates EGFR activity and alters EGFR-containing tumour-derived exosomes in head and neck cancers. | Additionally, ANXA1 modulation affected exosome production and influenced the release of exosomal phospho-EGFR. Gain- and loss-of-function studies were performed to analyse the effects of ANXA1 modulation on cell proliferation, mechanism of activation of EGFR signalling as well as on exosome production and exosomal phospho-EGFR. |
| 30893511 | Oncogenic and Non-Malignant Pancreatic Exosome Cargo Reveal Distinct Expression of Oncogenic and Prognostic Factors Involved in Tumor Invasion and Metastasis. | Importantly, valuable insights into oncogenic exosomes (362 unique proteins in comparison to non-malignant exosomes) of key metastatic regulatory factors and signaling molecules fundamental to pancreatic cancer progression (KRAS, CD44, EGFR) are provided. It is reported that oncogenic exosomes contain factors known to regulate the pre-metastatic niche (S100A4, F3, ITGβ5, ANXA1), clinically-relevant proteins which correlate with poor prognosis (CLDN1, MUC1) as well as protein networks involved in various cancer hallmarks including proliferation (CLU, CAV1), invasion (PODXL, ITGA3), metastasis (LAMP1, ST14) and immune surveillance escape (B2M). |
| 31331117 | Annexin A1 Contained in Extracellular Vesicles Promotes the Activation of Keratinocytes by Mesoglycan Effects: An Autocrine Loop Through FPRs. | No abstract available |
| 32894039 | The crosstalk of ABCA1 and ANXA1: a potential mechanism for protection against atherosclerosis. | ABCA1 transports ANXA1 from the cytoplasm to the cell membrane by regulating lipidization and serine phosphorylation, thereby mediating ANXA1 efflux, likely by promoting microparticle and exosome release. The latter activity occurs by mediating the efflux of ANXA1, which plays a critical role in anti-inflammatory effects, cholesterol transport, exosome and microparticle secretion, and apoptotic cell clearance. |
| 32938681 | Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis. | No abstract available |
| 33353163 | Annexin A1 Released in Extracellular Vesicles by Pancreatic Cancer Cells Activates Components of the Tumor Microenvironment, through Interaction with the Formyl-Peptide Receptors. | In PC, the protein Annexin A1 (ANXA1) appears to be overexpressed and may be identified as an oncogenic factor, also because it is a component in tumor-deriving extracellular vesicles (EVs). |
| 34779491 | Exosomal ANXA1 derived from thyroid cancer cells is associated with malignant transformation of human thyroid follicular epithelial cells by promoting cell proliferation. | Furthermore, ANXA1 was transferred from the SW579 cells to the Nthy‑ori3‑1 cells via exosomes. In addition, annexin A1 (ANXA1) is a well‑known oncogene, that can be released from extracellular vesicles by cancer cells. In the present study, the protein expression levels of ANXA1 in thyroid cancer cells and thyroid cancer cell‑derived exosomes were analyzed using western blot analysis. |
| 37862381 | Surfaceome analysis of extracellular vesicles from senescent cells uncovers uptake repressor DPP4. | No abstract available |
| 38375617 | Extracellular vesicles produced by 3D cultured MSCs promote wound healing by regulating macrophage activation through ANXA1. | No abstract available |