Protein detail
G3P
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-)
Protein symbol G3P | UniProt ID | EVMP score 0.50 |
Frequency 8 | Transmembrane count | Protein classification EnzymesEssential proteinsFDA approved drug targetsMetabolic proteinsPlasma proteinsPredicted intracellular proteins |
Basic Information
Protein Names
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-)
Protein Class
EnzymesEssential proteinsFDA approved drug targetsMetabolic proteinsPlasma proteinsPredicted intracellular proteins
Protein Function
- FDA approved drug targets:Small molecule drugs
- Enzymes
- ENZYME proteins:Oxidoreductases
- Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym
GAPD
Gene Description
Glyceraldehyde-3-phosphate dehydrogenase
Chromosome
12
Position
6534512-6538374
Frequency
8
EVMP Score
0.50
Fluorescence & Localization
Cell SpecificAdipocytes
Function & Pathway
Protein Function
- FDA approved drug targets:Small molecule drugs
- Enzymes
- ENZYME proteins:Oxidoreductases
- Predicted intracellular proteins
Cellular Component
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005811 lipid droplet
- GO:0005829 cytosol
- GO:0005886 plasma membrane
- GO:0015630 microtubule cytoskeleton
- GO:0016020 membrane
- GO:0031965 nuclear membrane
- GO:0031982 vesicle
- GO:0043231 intracellular membrane-bounded organelle
- GO:0048471 perinuclear region of cytoplasm
- GO:0070062 extracellular exosome
- GO:0097452 GAIT complex
- GO:1990904 ribonucleoprotein complex
Molecular Function
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
- GO:0005515 protein binding
- GO:0008017 microtubule binding
- GO:0019828 aspartic-type endopeptidase inhibitor activity
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0042802 identical protein binding
- GO:0050661 NADP binding
- GO:0051287 NAD binding
- GO:0097718 disordered domain specific binding
Biological Process
KEGG
- hsa00010 Glycolysis / Gluconeogenesis
- KEGG:hsa01100 Metabolic pathways
- KEGG:hsa01200 Carbon metabolism
- KEGG:hsa01230 Biosynthesis of amino acids
- KEGG:hsa04066 HIF-1 signaling pathway
- KEGG:hsa05010 Alzheimer disease
- KEGG:hsa05130 Pathogenic Escherichia coli infection
- KEGG:hsa05132 Salmonella infection
- KEGG:hsa05415 Diabetic cardiomyopathy
Reactome
Canonical Pathways
M68 Pid rhoa reg pathway
Mediation Categories
Clinical-translation mediationImmune mediationMetabolism mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
15 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| GAPDH | PRKAA1 | Q13131 | S | 122 | phosphorylation | SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper | SIGNOR:26626483 |
| GAPDH | PRKAA2 | P54646 | S | 122 | phosphorylation | SIGNOR | SIGNOR:26626483 |
| GAPDH | PRKAG1 | P54619 | S | 122 | phosphorylation | SIGNOR | SIGNOR:26626483 |
| GAPDH | PRKAB2 | O43741 | S | 122 | phosphorylation | SIGNOR | SIGNOR:26626483 |
| GAPDH | PRKAG3 | Q9UGI9 | S | 122 | phosphorylation | SIGNOR | SIGNOR:26626483 |
| GAPDH | PRKAB1 | Q9Y478 | S | 122 | phosphorylation | SIGNOR | SIGNOR:26626483 |
| GAPDH | PRKAG2 | Q9UGJ0 | S | 122 | phosphorylation | SIGNOR | SIGNOR:26626483 |
| GAPDH | PRKCD | Q05655 | T | 246 | phosphorylation | Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:25399565ProtMapper:25859407 |
| GAPDH | PRKCD | Q05655 | S | 241 | phosphorylation | PhosphoSite | |
| GAPDH | SRC | P12931 | Y | 42 | phosphorylation | BEL-Large-Corpus_ProtMapperPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperRLIMS-P_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:17488287 |
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Ligand-Receptor Signaling
4 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
Regulatory Interaction Network
8 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| FBW10 | Q5XX13 | G3P | P04406 | Yes | Yes | No | SIGNOR | SIGNOR:37450367 |
| AAPK1 | Q13131 | G3P | P04406 | Yes | Yes | No | Sparser_ProtMapperiPTMnetSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:26626483PhosphoSite:26626483PhosphoSite:18183946ProtMapper:32370188SIGNOR:26626483 |
| STK4 | Q13043 | G3P | P04406 | Yes | Yes | No | SIGNOR | SIGNOR:23527007 |
| VRK2 | Q86Y07 | G3P | P04406 | Yes | Yes | No | SIGNOR | SIGNOR:37450367 |
| COMPLEX:O43741_P54619_P54646_Q13131_Q9UGI9_Q9UGJ0_Q9Y478 | G3P | P04406 | Yes | Yes | No | SIGNOR | SIGNOR:26626483 | |
| KPCD | Q05655 | G3P | P04406 | Yes | No | No | Sparser_ProtMapperPhosphoSite_norefiPTMnetProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:25859407ProtMapper:25399565PhosphoSite:23653351PhosphoSite:27129213 |
| AKT2 | P31751 | G3P | P04406 | Yes | No | No | Sparser_ProtMapperPhosphoSite_MIMPMIMPiPTMnetProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:21979951PhosphoSite:21979951ProtMapper:25859407 |
| SRC | P12931 | G3P | P04406 | Yes | No | No | BEL-Large-Corpus_ProtMapperPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:17488287ProtMapper:17488287ProtMapper:32539222 |
Protein Complex Composition
16 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| GAPDHPGK1 | P00558P04406 | 0:0 | hu.MAP | |||
| GAPDHPGAM1PGAM2PGK1 | P00558P04406P15259P18669 | 0:0:0:0 | hu.MAP | |||
| GAPDH | P04406 | 4 | PDB | PDB:6iq6PDB:8g14PDB:8dnsPDB:6ynePDB:8g15PDB:3gpdPDB:1u8fPDB:1znqPDB:9l3ePDB:8g13PDB:8p5fPDB:4wncPDB:8g17PDB:8g12PDB:6yndPDB:6adePDB:6ynfPDB:4wniPDB:6m61PDB:8g16PDB:6ynh | ||
| GAPDHPGAM1 | P04406P18669 | 0:0 | hu.MAP | |||
| GAPDHNIT2PGAM1SCP2 | P04406P18669P22307Q9NQR4 | 0:0:0:0 | hu.MAP | |||
| GAPDHNIT2 | P04406Q9NQR4 | 0:0 | hu.MAP |
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Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Western blotting | 1 | 37922300 |
Sequence, Structure & Domains
Sequences
Length
335
Mass
36,053
Sequence
MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P04406-1; Sequence=Displayed; Name=2; IsoId=P04406-2; Sequence=VSP_047289
Alternative Sequence
1..42; Missing (in isoform 2)
3D Structural Models
Turn
51..53; 133..135; 202..204; 268..273; 297..299
Helix
13..25; 40..48; 82..84; 88..90; 105..108; 110..113; 137..139; 152..168; 196..199; 213..220; 222..224; 255..267; 283..286; 318..333
Beta Strand
5..9; 27..34; 36..38; 60..63; 66..69; 72..77; 94..97; 99..101; 117..123; 126..128; 146..148; 170..179; 185..189; 207..210; 227..234; 241..251; 274..277; 292..296; 301..304; 307..314
3D Structure
Electron microscopy (7); X-ray crystallography (14)
Domain & Motif Annotations
Motif
245..250; [IL]-x-C-x-x-[DE] motif
Domain (CC)
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
Region
2..148; Interaction with WARS1
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Sequence Similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Clinical Relevance
Disease Involvement
FDA approved drug targets
Related Diseases
Biomarker
Phase 2; Phase 1
Drug Targets
FDA approved drug targets
Drugs
CHEMBL:CHEMBL502775SELENIUMETHOXZOLAMIDEDORZOLAMIDEACETAZOLAMIDECHLORTHALIDONECHEMBL:CHEMBL429095ANESTHETIC AGENTTAMOXIFENEPINEPHRINESODIUM BUTYRATEGALLIC ACIDSULPIRIDECHEMBL:CHEMBL601757BRINZOLAMIDEBENZOLAMIDECHEMBL:CHEMBL580421NULLHISTAMINEINDISULAMTOPIRAMATEESTRADIOL VALERATEOMIGAPILMETHOCARBAMOLCHEMBL:CHEMBL578512METHAZOLAMIDEHISTIDINEZONISAMIDEPURPUROGALLINCHEMBL:CHEMBL505670DICHLORPHENAMIDECOMPOUND 5A [PMID: 31287314]PROTEIN SYNTHESIS INHIBITORSLC-0111THIRAMCHEMBL:CHEMBL1399359,10-PHENANTHRENEQUINONEDEFEROXAMINECOMPOUND 5B [PMID: 31287314]
Interaction Protein
ENSG00000082701ENSG00000102144ENSG00000117748ENSG00000130707ENSG00000132646ENSG00000139684ENSG00000146648ENSG00000152700ENSG00000167900
Interaction Count
9
Interaction Dataset
intact_biogridbiogrid_opencell