Protein detail

GNAI2

Guanine nucleotide-binding protein G(i) subunit alpha-2 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)

Entry name GNAI2	UniProt ID P04899	EVMP confidence score 0.75
Supporting publications (n) 45	Transmembrane count	Protein classification Human disease related genesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Guanine nucleotide-binding protein G(i) subunit alpha-2 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)

Protein Class (2)

Human disease related genesPredicted intracellular proteins

Protein Function (3)

Human disease related genes:Cancers:Cancers of endocrine organs
Predicted intracellular proteins
Human disease related genes:Cardiovascular diseases:Cardiac diseases

Ensembl

ENSG00000114353

Entrez Gene Symbol

GNAI2

Gene Synonym (2)

GIPGNAI2B

Gene Description

G protein subunit alpha i2

Chromosome

Position

50226292-50259362

Supporting publications (n)

EVMP confidence score

0.75

Fluorescence & Localization3

Cell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophage

Function & Pathway8

Protein Function (3)

Human disease related genes:Cancers:Cancers of endocrine organs
Predicted intracellular proteins
Human disease related genes:Cardiovascular diseases:Cardiac diseases

Cellular Component (14)

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Molecular Function (6)

Biological Process (3)

KEGG (43)

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Reactome (27)

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Canonical Pathways (3)

M5060 Sa fas signaling
M79 Pid trail pathway
M197 Pid hiv nef pathway

Mediation Categories (5)

Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence43

Enzyme-Mediated Modification (3)

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database
GNAI2	PRKCE	Q02156	S	44	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
GNAI2	PRKCE	Q02156	S	144	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
GNAI2	PRKCE	Q02156	S	302	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper

Ligand-Receptor Signaling (19)

19 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	No	No
adhesion	adhesion	OmniPath	Yes	Yes	No	No	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	Cellinker	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
cell_surface	cell_surface	connectomeDB2020	No	No	No	No	No
cell_surface	cell_surface	Baccin2019	No	No	No	No	No
cell_surface	cell_surface	OmniPath	No	No	No	No	No

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Regulatory Interaction Network (21)

21 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ARRB2	P32121	GNAI2	P04899	Yes	No	Yes	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GNAI2	P04899	GNB5	O14775	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:12495636 SignaLink3:10508237
GNAI2	P04899	GBG4	P50150	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:12495636 SignaLink3:10508237
GNAI2	P04899	FGR	P09769	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:10753828 ProtMapper:11160627 SignaLink3:23109003 SignaLink3:11007482 SignaLink3:9722546
GNAI2	P04899	HCK	P08631	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:10753828 ProtMapper:11160627 SignaLink3:23109003 SignaLink3:11007482 SignaLink3:9722546
GNAI2	P04899	LYN	P07948	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:10753828 ProtMapper:11160627 SignaLink3:23109003 SignaLink3:11007482 SignaLink3:9722546
GNAI2	P04899	SRC	P12931	Yes	Yes	No	NCI-PID_ProtMapperProtMapperSignaLink3WangTCRcuration_SignaLink3	SignaLink3:10753828 ProtMapper:11160627 SignaLink3:23109003 SignaLink3:11007482 SignaLink3:9722546
GNAI2	P04899	ADCY1	Q08828	Yes	No	Yes	Fantom5_LRdbCellTalkDBHPRD_LRdbiTALKKEGG-MEDICUStalklrSIGNORconnectomeDB2020HPRDHPRD_talklrBaccin2019Ramilowski2015_Baccin2019WangLRdbSTRING_talklrEMBRACERamilowski2015	SIGNOR:19703466 SIGNOR:8327893 CellTalkDB:2121366 HPRD:2121366 LRdb:2121366 connectomeDB2020:2121366 Baccin2019:2121366
SMO	Q99835	GNAI2	P04899	Yes	Yes	No	WangSIGNOR	SIGNOR:16885213 SIGNOR:23074268
GNAI2	P04899	TFIP8	O95379	Yes	Yes	No	SIGNOR	SIGNOR:20607800

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Sequence, Structure & Domains13

Sequences

Length

355

Mass

40,451

Sequence

MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Alternative Products

Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=P04899-1; Sequence=Displayed; Name=2; IsoId=P04899-2; Sequence=VSP_017497; Name=3; IsoId=P04899-3; Sequence=VSP_043473; Name=sGi2; Synonyms=sGalphai2; IsoId=P04899-4; Sequence=VSP_043903; Name=5; IsoId=P04899-5; Sequence=VSP_054789; Name=6; IsoId=P04899-6; Sequence=VSP_054788

Alternative Sequence

1..52; Missing (in isoform 6); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MEYAGHLPASSAQGTILACTSCT (in isoform 2); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MR (in isoform 3); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MTEGVKTLGWTKQKGGCHWGRSE (in isoform 5); 332..355; NVQFVFDAVTDVIIKNNLKDCGLF -> SRKLFRETYLKLSGPDQHPHPSPAPAPPLSSDSVP (in isoform sGi2)

3D Structural Models

Turn

51..53; 209..212; 236..238; 273..275; 285..287; 299..301; 315..317; 329..331

Helix

7..30; 46..48; 213..216; 228..230; 243..255; 258..260; 276..281; 302..310; 332..351

Beta Strand

33..39; 42..44; 186..191; 196..201; 220..227; 231..233; 239..242; 264..270; 296..298; 320..324

3D Structure

Electron microscopy (25)

Domain & Motif Annotations

Domain (FT)

32..355; G-alpha

Region

35..48; G1 motif; 174..182; G2 motif; 197..206; G3 motif; 266..273; G4 motif; 325..330; G5 motif

Protein Families (2)

G-alpha family
G(i/o/t/z) subfamily

Sequence Similarities

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Clinical Relevance6

Drug Targets

Clinical trial target

Drugs (19)

VENADAPARIB AG14361 SARUPARIB DUAL VEGFR/PARP INHIBITOR 14B PAMIPARIB COMPOUND 5 [TOMASSI ET AL., 2020]NIRAPARIB LERZEPARIB AZ1366 PJ34 ME0328 AZD9574 COMPOUND 10B [PMID: 26222319]OLAPARIB COMPOUND 9 [LIN ET AL., 2023]RUCAPARIB CICLOPIROX MC2050 TALAZOPARIB

Antibody

HPA007704

Interaction Protein (3)

ENSG00000078369ENSG00000112559ENSG00000160683

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications43

PMID	Title	Abstract
25755179	Urinary extracellular vesicles as reservoirs of altered proteins during the pathogenesis of polycystic kidney disease.	No abstract available
26826536	[Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].	No abstract available
27487081	Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation.	No abstract available
28680152	Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: their emerging role in tumor heterogeneity.	No abstract available
30915084	Extracellular Vesicles Mediate Mesenchymal Stromal Cell-Dependent Regulation of B Cell PI3K-AKT Signaling Pathway and Actin Cytoskeleton.	No abstract available
31617158	Proteomic profiling of peritoneal dialysis effluent-derived extracellular vesicles: a longitudinal study.	No abstract available
31805958	Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.	No abstract available
32230970	Radiation Exposure of Peripheral Mononuclear Blood Cells Alters the Composition and Function of Secreted Extracellular Vesicles.	No abstract available
32560723	T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.	No abstract available
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.

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