Protein detail
GNAI2
Guanine nucleotide-binding protein G(i) subunit alpha-2 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)
Entry name GNAI2 | UniProt ID | EVMP confidence score 0.75 |
Supporting publications (n) 45 | Transmembrane count | Protein classification Human disease related genesPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Guanine nucleotide-binding protein G(i) subunit alpha-2 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)
Protein Class (2)
Human disease related genesPredicted intracellular proteins
Protein Function (3)
- Human disease related genes:Cancers:Cancers of endocrine organs
- Predicted intracellular proteins
- Human disease related genes:Cardiovascular diseases:Cardiac diseases
Ensembl
Entrez Gene Symbol
Gene Synonym (2)
GIPGNAI2B
Gene Description
G protein subunit alpha i2
Chromosome
3
Position
50226292-50259362
Supporting publications (n)
45
EVMP confidence score
0.75
Fluorescence & Localization3
Cell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophage
Function & Pathway8
Protein Function (3)
- Human disease related genes:Cancers:Cancers of endocrine organs
- Predicted intracellular proteins
- Human disease related genes:Cardiovascular diseases:Cardiac diseases
Cellular Component (14)
- GO:0005654 nucleoplasm
- GO:0005737 cytoplasm
- GO:0005813 centrosome
- GO:0005829 cytosol
- GO:0005834 heterotrimeric G-protein complex
- GO:0005886 plasma membrane
- GO:0016020 membrane
- GO:0030425 dendrite
- GO:0030496 midbody
- GO:0044297 cell body
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Molecular Function (6)
Biological Process (3)
KEGG (43)
- hsa04015 Rap1 signaling pathway
- KEGG:hsa04022 cGMP-PKG signaling pathway
- KEGG:hsa04024 cAMP signaling pathway
- KEGG:hsa04062 Chemokine signaling pathway
- KEGG:hsa04071 Sphingolipid signaling pathway
- KEGG:hsa04081 Hormone signaling
- KEGG:hsa04082 Neuroactive ligand signaling
- KEGG:hsa04261 Adrenergic signaling in cardiomyocytes
- KEGG:hsa04360 Axon guidance
- KEGG:hsa04371 Apelin signaling pathway
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Reactome (27)
- R-hsa-170670 adenylate cyclase inhibitory pathway
- R-hsa-9660821 adora2b mediated anti inflammatory cytokines production
- R-hsa-392170 adp signalling through p2y purinoceptor 12
- R-hsa-400042 adrenaline noradrenaline inhibits insulin secretion
- R-hsa-9662851 anti inflammatory response favouring leishmania parasite infection
- R-hsa-8939211 esr mediated signaling
- R-hsa-9009391 extra nuclear estrogen signaling
- R-hsa-977444 gaba b receptor activation
- R-hsa-977443 gaba receptor activation
- R-hsa-9634597 gper1 signaling
Page 1 of 3
Canonical Pathways (3)
- M5060 Sa fas signaling
- M79 Pid trail pathway
- M197 Pid hiv nef pathway
Mediation Categories (5)
Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation
Relations & Evidence43
Enzyme-Mediated Modification (3)
3 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| GNAI2 | PRKCE | Q02156 | S | 44 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| GNAI2 | PRKCE | Q02156 | S | 144 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| GNAI2 | PRKCE | Q02156 | S | 302 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper |
Ligand-Receptor Signaling (19)
19 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | Baccin2019 | No | Yes | No | No | No |
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | connectomeDB2020 | Yes | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | Baccin2019 | Yes | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | OmniPath | Yes | No | No | No | No |
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Regulatory Interaction Network (21)
21 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| KPCE | Q02156 | GNAI2 | P04899 | Yes | No | No | PhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:20043990 |
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Sequence, Structure & Domains13
Sequences
Length
355
Mass
40,451
Sequence
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Alternative Products
Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=P04899-1; Sequence=Displayed; Name=2; IsoId=P04899-2; Sequence=VSP_017497; Name=3; IsoId=P04899-3; Sequence=VSP_043473; Name=sGi2; Synonyms=sGalphai2; IsoId=P04899-4; Sequence=VSP_043903; Name=5; IsoId=P04899-5; Sequence=VSP_054789; Name=6; IsoId=P04899-6; Sequence=VSP_054788
Alternative Sequence
1..52; Missing (in isoform 6); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MEYAGHLPASSAQGTILACTSCT (in isoform 2); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MR (in isoform 3); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MTEGVKTLGWTKQKGGCHWGRSE (in isoform 5); 332..355; NVQFVFDAVTDVIIKNNLKDCGLF -> SRKLFRETYLKLSGPDQHPHPSPAPAPPLSSDSVP (in isoform sGi2)
3D Structural Models
Turn
51..53; 209..212; 236..238; 273..275; 285..287; 299..301; 315..317; 329..331
Helix
7..30; 46..48; 213..216; 228..230; 243..255; 258..260; 276..281; 302..310; 332..351
Beta Strand
33..39; 42..44; 186..191; 196..201; 220..227; 231..233; 239..242; 264..270; 296..298; 320..324
3D Structure
Electron microscopy (25)
Domain & Motif Annotations
Domain (FT)
32..355; G-alpha
Region
35..48; G1 motif; 174..182; G2 motif; 197..206; G3 motif; 266..273; G4 motif; 325..330; G5 motif
Protein Families (2)
- G-alpha family
- G(i/o/t/z) subfamily
Sequence Similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily.
Clinical Relevance6
Drug Targets
Clinical trial target
Drugs (19)
Antibody
Interaction Protein (3)
ENSG00000078369ENSG00000112559ENSG00000160683
Interaction Count
3
Interaction Dataset
intact_biogrid
Supporting Publications43
| PMID | Title | Abstract |
|---|---|---|
| 32916986 | Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines. | No abstract available |
| 33309826 | Proteomic analysis of extracellular vesicles and conditioned medium from human adipose-derived stem/stromal cells and dermal fibroblasts. | No abstract available |
| 33592500 | A Proteomic Approach to Understand the Clinical Significance of Acute Myeloid Leukemia-Derived Extracellular Vesicles Reflecting Essential Characteristics of Leukemia. | No abstract available |
| 34064677 | Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells. | No abstract available |
| 34473505 | Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles. | No abstract available |
| 34576246 | Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators. | No abstract available |
| 34599510 | Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease. | In particular, an increase in the amount of S100A8 and S100A9 carried by exosomes of severe pancreatitis suggests that the mechanism of action of exosomes is mediated by the effect of these proteins on NADPH oxidase. |
| 36064647 | Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells. | No abstract available |
| 36146834 | Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. | No abstract available |
| 36497184 | Oxidative Stress and Extracellular Matrix Remodeling Are Signature Pathways of Extracellular Vesicles Released upon Morphine Exposure on Human Brain Microvascular Endothelial Cells. | No abstract available |