Protein detail
GNAI2
Guanine nucleotide-binding protein G(i) subunit alpha-2 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)
Entry name GNAI2 | UniProt ID | EVMP confidence score 0.75 |
Supporting publications (n) 45 | Transmembrane count | Protein classification Human disease related genesPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Guanine nucleotide-binding protein G(i) subunit alpha-2 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)
Protein Class (2)
Human disease related genesPredicted intracellular proteins
Protein Function (3)
- Human disease related genes:Cancers:Cancers of endocrine organs
- Predicted intracellular proteins
- Human disease related genes:Cardiovascular diseases:Cardiac diseases
Ensembl
Entrez Gene Symbol
Gene Synonym (2)
GIPGNAI2B
Gene Description
G protein subunit alpha i2
Chromosome
3
Position
50226292-50259362
Supporting publications (n)
45
EVMP confidence score
0.75
Fluorescence & Localization3
Cell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophage
Function & Pathway8
Protein Function (3)
- Human disease related genes:Cancers:Cancers of endocrine organs
- Predicted intracellular proteins
- Human disease related genes:Cardiovascular diseases:Cardiac diseases
Cellular Component (14)
- GO:0005654 nucleoplasm
- GO:0005737 cytoplasm
- GO:0005813 centrosome
- GO:0005829 cytosol
- GO:0005834 heterotrimeric G-protein complex
- GO:0005886 plasma membrane
- GO:0016020 membrane
- GO:0030425 dendrite
- GO:0030496 midbody
- GO:0044297 cell body
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Molecular Function (6)
Biological Process (3)
KEGG (43)
- hsa04015 Rap1 signaling pathway
- KEGG:hsa04022 cGMP-PKG signaling pathway
- KEGG:hsa04024 cAMP signaling pathway
- KEGG:hsa04062 Chemokine signaling pathway
- KEGG:hsa04071 Sphingolipid signaling pathway
- KEGG:hsa04081 Hormone signaling
- KEGG:hsa04082 Neuroactive ligand signaling
- KEGG:hsa04261 Adrenergic signaling in cardiomyocytes
- KEGG:hsa04360 Axon guidance
- KEGG:hsa04371 Apelin signaling pathway
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Reactome (27)
- R-hsa-170670 adenylate cyclase inhibitory pathway
- R-hsa-9660821 adora2b mediated anti inflammatory cytokines production
- R-hsa-392170 adp signalling through p2y purinoceptor 12
- R-hsa-400042 adrenaline noradrenaline inhibits insulin secretion
- R-hsa-9662851 anti inflammatory response favouring leishmania parasite infection
- R-hsa-8939211 esr mediated signaling
- R-hsa-9009391 extra nuclear estrogen signaling
- R-hsa-977444 gaba b receptor activation
- R-hsa-977443 gaba receptor activation
- R-hsa-9634597 gper1 signaling
Page 1 of 3
Canonical Pathways (3)
- M5060 Sa fas signaling
- M79 Pid trail pathway
- M197 Pid hiv nef pathway
Mediation Categories (5)
Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation
Relations & Evidence43
Enzyme-Mediated Modification (3)
3 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| GNAI2 | PRKCE | Q02156 | S | 44 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| GNAI2 | PRKCE | Q02156 | S | 144 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| GNAI2 | PRKCE | Q02156 | S | 302 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper |
Ligand-Receptor Signaling (19)
19 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | Baccin2019 | No | Yes | No | No | No |
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | connectomeDB2020 | Yes | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | Baccin2019 | Yes | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | OmniPath | Yes | No | No | No | No |
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Regulatory Interaction Network (21)
21 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| KPCE | Q02156 | GNAI2 | P04899 | Yes | No | No | PhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:20043990 |
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Sequence, Structure & Domains13
Sequences
Length
355
Mass
40,451
Sequence
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Alternative Products
Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=P04899-1; Sequence=Displayed; Name=2; IsoId=P04899-2; Sequence=VSP_017497; Name=3; IsoId=P04899-3; Sequence=VSP_043473; Name=sGi2; Synonyms=sGalphai2; IsoId=P04899-4; Sequence=VSP_043903; Name=5; IsoId=P04899-5; Sequence=VSP_054789; Name=6; IsoId=P04899-6; Sequence=VSP_054788
Alternative Sequence
1..52; Missing (in isoform 6); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MEYAGHLPASSAQGTILACTSCT (in isoform 2); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MR (in isoform 3); 1..39; MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL -> MTEGVKTLGWTKQKGGCHWGRSE (in isoform 5); 332..355; NVQFVFDAVTDVIIKNNLKDCGLF -> SRKLFRETYLKLSGPDQHPHPSPAPAPPLSSDSVP (in isoform sGi2)
3D Structural Models
Turn
51..53; 209..212; 236..238; 273..275; 285..287; 299..301; 315..317; 329..331
Helix
7..30; 46..48; 213..216; 228..230; 243..255; 258..260; 276..281; 302..310; 332..351
Beta Strand
33..39; 42..44; 186..191; 196..201; 220..227; 231..233; 239..242; 264..270; 296..298; 320..324
3D Structure
Electron microscopy (25)
Domain & Motif Annotations
Domain (FT)
32..355; G-alpha
Region
35..48; G1 motif; 174..182; G2 motif; 197..206; G3 motif; 266..273; G4 motif; 325..330; G5 motif
Protein Families (2)
- G-alpha family
- G(i/o/t/z) subfamily
Sequence Similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily.
Clinical Relevance6
Drug Targets
Clinical trial target
Drugs (19)
Antibody
Interaction Protein (3)
ENSG00000078369ENSG00000112559ENSG00000160683
Interaction Count
3
Interaction Dataset
intact_biogrid
Supporting Publications43
| PMID | Title | Abstract |
|---|---|---|
| 36982312 | Saliva and Saliva Extracellular Vesicles for Biomarker Candidate Identification-Assay Development and Pilot Study in Amyotrophic Lateral Sclerosis. | No abstract available |
| 37204515 | Proteomic profiling and functional characterization of serum-derived extracellular vesicles in the mucinous and non-mucinous colon adenocarcinoma. | No abstract available |
| 37322475 | Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration. | No abstract available |
| 37438638 | Extracellular Vesicles Play a Central Role in Cerebral Venous Disease-Associated Brain Atrophy. | No abstract available |
| 37563857 | Comprehensive characterization of human brain-derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applications. | No abstract available |
| 37670049 | Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility. | No abstract available |
| 37926756 | Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells. | No abstract available |
| 38014595 | Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia. | No abstract available |
| 38113368 | In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum. | No abstract available |
| 38207106 | Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity. | No abstract available |