Protein detail
ENOA
Alpha-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein)
Entry name ENOA | UniProt ID | EVMP confidence score 0.38 |
Supporting publications (n) 4 | Transmembrane count | Protein classification Cancer-related genesEnzymesMetabolic proteinsPlasma proteinsPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Alpha-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein)
Protein Class (5)
Cancer-related genesEnzymesMetabolic proteinsPlasma proteinsPredicted intracellular proteins
Protein Function (4)
- Enzymes
- Cancer-related genes:Candidate cancer biomarkers
- Predicted intracellular proteins
- ENZYME proteins:Lyases
Ensembl
Entrez Gene Symbol
Gene Synonym (4)
ENO1L1MBP-1MPB1PPH
Gene Description
Enolase 1
Chromosome
1
Position
8861000-8879190
Supporting publications (n)
4
EVMP confidence score
0.38
Function & Pathway7
Protein Function (4)
- Enzymes
- Cancer-related genes:Candidate cancer biomarkers
- Predicted intracellular proteins
- ENZYME proteins:Lyases
Cellular Component (12)
- GO:0000015 phosphopyruvate hydratase complex
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0005640 nuclear outer membrane
- GO:0005737 cytoplasm
- GO:0005829 cytosol
- GO:0005886 plasma membrane
- GO:0005938 cell cortex
- GO:0009986 cell surface
- GO:0016020 membrane
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Molecular Function (11)
- GO:0000287 magnesium ion binding
- GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
- GO:0001222 transcription corepressor binding
- GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
- GO:0003714 transcription corepressor activity
- GO:0003723 RNA binding
- GO:0004634 phosphopyruvate hydratase activity
- GO:0005515 protein binding
- GO:0042803 protein homodimerization activity
- GO:0045296 cadherin binding
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Biological Process (3)
KEGG (7)
Reactome (7)
Mediation Categories (3)
Clinical-translation mediationFusion and delivery mediationMetabolism mediation
Relations & Evidence36
Enzyme-Mediated Modification (9)
9 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| ENO1 | SRC | P12931 | Y | 44 | phosphorylation | BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapper | ProtMapper:15212693KEA:8578591SIGNOR:7629021KEA:15951569phosphoELM:8578591KEA:15592455KEA:96108162ProtMapper:24841372ProtMapper:7629021SIGNOR:24841372 |
| ENO1 | ULK1 | O75385 | S | 282 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper | |
| ENO1 | ULK1 | O75385 | S | 115 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper | |
| ENO1 | RPS6KA4 | O75676 | S | 37 | phosphorylation | PhosphoNetworks | |
| ENO1 | STK17A | Q9UEE5 | S | 37 | phosphorylation | PhosphoNetworks | |
| ENO1 | YES1 | P07947 | Y | 44 | phosphorylation | PhosphoNetworks | |
| ENO1 | GRK5 | P34947 | T | 100 | phosphorylation | PhosphoNetworks | |
| ENO1 | GRK5 | P34947 | S | 254 | phosphorylation | PhosphoNetworks | |
| ENO1 | GRK5 | P34947 | S | 419 | phosphorylation | PhosphoNetworks |
Ligand-Receptor Signaling (13)
13 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| cell_surface | cell_surface | connectomeDB2020 | No | No | No | No | No |
| cell_surface | cell_surface | OmniPath | No | No | No | No | No |
| receptor | receptor | connectomeDB2020 | No | Yes | No | No | No |
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Regulatory Interaction Network (3)
3 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| ULK1 | O75385 | ENOA | P06733 | Yes | No | Yes | PhosphoSite_norefSIGNORPhosphoSite_ProtMapperProtMapper | SIGNOR:27153534 |
| SRC | P12931 | ENOA | P06733 | Yes | Yes | No | BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperHINTPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapper | HINT:15456896ProtMapper:15212693HINT:11078745KEA:8578591SIGNOR:7629021KEA:15951569phosphoELM:8578591HINT:17525734KEA:15592455KEA:96108162ProtMapper:24841372HINT:16609991ProtMapper:7629021SIGNOR:24841372 |
| ULK2 | Q8IYT8 | ENOA | P06733 | Yes | No | Yes | SIGNOR | SIGNOR:27153534 |
Protein Complex Composition (10)
10 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| ENO1PCMT1TKTTPI1YDJC | A8MPS7P06733P22061P29401P60174 | 0:0:0:0:0 | hu.MAP | |||
| AP3B1AP3D1AP3M1AP3S1AP3S2ENO1HCCSPUS1UBCVAMP7 | O00203O14617P06733P0CG48P51809P53701P59780Q92572Q9Y2T2Q9Y606 | 1:1:1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC5159 | ||
| ALDOAENO1YKT6 | O15498P04075P06733 | 0:0:0 | hu.MAP2 | |||
| ENO1 | P06733 | 4 | PDB | PDB:2psnPDB:3b97 | ||
| ENO1GPIGSTO1PARK7PCMT1TKT | P06733P06744P22061P29401P78417Q99497 | 0:0:0:0:0:0 | hu.MAP | |||
| ENO1PGK2TBC1D13TVP23BUBC | P06733P07205P0CG48Q9NVG8Q9NYZ1 | 1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC7304 | ||
| ALDOCDYNLRB2ENO1FKBP7IRS1SPR | P06733P09972P35270P35568Q8TF09Q9Y680 | 0:0:0:0:0:0 | hu.MAP2 | |||
| CMPK1ENO1TPI1 | P06733P30085P60174 | 0:0:0 | hu.MAP | |||
| ENO1ITPAPPIATAGLN2 | P06733P37802P62937Q9BY32 | 0:0:0:0 | Havugimana2012 | Havugimana2012:C_287 | ||
| ENO1TPI1 | P06733P60174 | 0:0 | hu.MAP |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Western blotting | 1 | 37922300 |
Sequence, Structure & Domains12
Sequences
Length
434
Mass
47,169
Sequence
MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
Alternative Products
Event=Alternative initiation; Named isoforms=2; Name=alpha-enolase; IsoId=P06733-1; Sequence=Displayed; Name=MBP-1; IsoId=P06733-2; Sequence=VSP_018725
Alternative Sequence
1..93; Missing (in isoform MBP-1)
3D Structural Models
Turn
104..106; 237..239; 259..262; 319..323
Helix
57..59; 63..71; 73..79; 87..98; 108..125; 130..138; 156..158; 178..200; 202..204; 220..233; 248..250; 267..269; 273..286; 301..311; 325..334; 344..347; 350..362; 380..387; 401..417; 418..420; 425..427
Beta Strand
5..12; 18..26; 29..34; 147..154; 159..162; 167..171; 241..245; 289..293; 313..318; 338..342; 366..370; 391..394
3D Structure
X-ray crystallography (8)
Domain & Motif Annotations
Region
31..38; Epitope recognized by CAR and healthy patient antibodies; 56..63; Epitope recognized by CAR antibodies; 97..237; Required for repression of c-myc promoter activity; 405..434; Required for interaction with PLG
Protein Families
Enolase family
Sequence Similarities
Belongs to the enolase family.
Clinical Relevance6
Disease Involvement
Cancer-related genes
Interaction Protein (5)
ENSG00000108515ENSG00000111674ENSG00000132646ENSG00000164924ENSG00000165458
Interaction Count
5
Interaction Dataset (4)
intact_biogrid_opencellintact_biogrid_bioplexintact_biogridbiogrid_opencell
Supporting Publications4
| PMID | Title | Abstract |
|---|---|---|
| 28986585 | Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics. | No abstract available |
| 32489530 | Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins. | No abstract available |
| 37786918 | Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples. | No abstract available |
| 38321535 | Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles. | No abstract available |