Protein detail

CXB1

Gap junction beta-1 protein (Connexin-32) (Cx32) (GAP junction 28 kDa liver protein)

Entry name CXB1	UniProt ID P08034	EVMP confidence score 0.38
Supporting publications (n)	Transmembrane count 4	Protein classification Disease related genesHuman disease related genesPotential drug targetsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information12

Protein Names

Gap junction beta-1 protein (Connexin-32) (Cx32) (GAP junction 28 kDa liver protein)

Protein Class (5)

Disease related genesHuman disease related genesPotential drug targetsPredicted membrane proteinsTransporters

Protein Function (4)

Human disease related genes:Nervous system diseases:Neurodegenerative diseases
Disease related genes
Transporters:Transporter channels and pores
Potential drug targets

Transmembrane

23..45; Helical; 76..95; Helical; 131..153; Helical; 192..214; Helical

Transmembrane Count

Ensembl

ENSG00000169562

Entrez Gene Symbol

GJB1

Gene Synonym (3)

CMTXCMTX1CX32

Gene Description

Gap junction protein beta 1

Chromosome

Position

71212811-71225516

EVMP confidence score

0.38

Fluorescence & Localization5

Tissue Specificchoroid plexusCell SpecificAdrenal cortex cellsSingle-Nuclei Brain SpecificBergmann gliaBlood Cell Specificintermediate monocyteBlood Lineage Specificmonocytes

Function & Pathway6

Protein Function (4)

Human disease related genes:Nervous system diseases:Neurodegenerative diseases
Disease related genes
Transporters:Transporter channels and pores
Potential drug targets

Cellular Component (3)

Molecular Function (3)

Biological Process (3)

Reactome (6)

Mediation Categories

Fusion and delivery mediation

Relations & Evidence49

Enzyme-Mediated Modification (18)

18 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
GJB1	PRKACA	P17612	S	233	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	ProtMapper:8390988 HPRD:8390988 KEA:8390988 SIGNOR:8390988
GJB1	PRKCA	P17252	S	229	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	ProtMapper:8390988 HPRD:8390988 KEA:8390988 SIGNOR:8390988
GJB1	PRKCA	P17252	S	233	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	ProtMapper:8390988 HPRD:8390988 KEA:8390988 SIGNOR:8390988
GJB1	EPHA1	P21709	Y	243	phosphorylation	PhosphoSite
GJB1	MST1R	Q04912	Y	243	phosphorylation	PhosphoSite
GJB1	EPHB1	P54762	Y	243	phosphorylation	PhosphoSite
GJB1	PRKCB	P05771	S	229	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA	KEA:17570479
GJB1	PRKCB	P05771	S	233	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
GJB1	PRKCG	P05129	S	229	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
GJB1	PRKCG	P05129	S	233	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP

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Ligand-Receptor Signaling (26)

26 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
cell_surface_ligand	cell_surface_ligand	Baccin2019	Yes	No	No	No	No
cell_surface_ligand	cell_surface_ligand	OmniPath	Yes	No	No	No	No
gap_junction	gap_junction	GO_Intercell	Yes	Yes	No	No	No
gap_junction	gap_junction	Ramilowski_location	Yes	Yes	No	No	No
gap_junction	gap_junction	UniProt_location	Yes	Yes	No	No	No
gap_junction	gap_junction	Almen2009	Yes	Yes	No	No	No

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Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KAPCA	P17612	CXB1	P08034	Yes	No	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD:8390988 HPRD-phos:8390988 ProtMapper:8390988 KEA:8390988 SIGNOR:8390988
KPCA	P17252	CXB1	P08034	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD:8390988 HPRD-phos:8390988 ProtMapper:8390988 KEA:8390988 SIGNOR:8390988
RON	Q04912	CXB1	P08034	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:30401746

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	GJB1	P08034	12	PDB	PDB:7zxtPDB:8qkiPDB:8qk6PDB:8qjfPDB:8qjhPDB:7zxnPDB:7zxpPDB:7zxmPDB:7zxqPDB:7zxo

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	14	32284825 32341357 33204424 35611462 32944193 34623918 40748658 28986585 30950185 31941606 38321535 39558134 29148239 30944935

Sequence, Structure & Domains9

Sequences

Length

283

Mass

32,025

Sequence

MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDQFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCDVYPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

3D Structural Models

Turn

41..43; 45..50

Helix

3..11; 19..40; 60..68; 73..102; 130..156; 184..216

Beta Strand

52..54; 159..161; 164..168; 173..175; 177..180

3D Structure

Electron microscopy (10); NMR spectroscopy (1)

Domain & Motif Annotations

Protein Families (2)

Connexin family
Beta-type (group I) subfamily

Sequence Similarities

Belongs to the connexin family. Beta-type (group I) subfamily.

Clinical Relevance3

Disease Involvement (5)

Charcot-Marie-Tooth diseaseDejerine-Sottas syndromeDisease variantNeurodegenerationNeuropathy

Drugs (4)

FLUFENAMIC ACID OCTANOL CA2+CARBENOXOLONE

Antibody

CAB012994