Protein detail

VILI

Villin-1

Entry name
VILI
UniProt ID
EVMP score
0.38
Frequency
2
Transmembrane count
Protein classification
Cancer-related genesDisease related genesPlasma proteinsPredicted intracellular proteins
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Villin-1
Protein Class
Cancer-related genesDisease related genesPlasma proteinsPredicted intracellular proteins
Protein Function
  • Disease related genes
  • Cancer-related genes:Candidate cancer biomarkers
  • Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym
D2S1471VIL
Gene Description
Villin 1
Chromosome
2
Position
218419121-218453295
Frequency
2
EVMP Score
0.38
Fluorescence & Localization
Cell SpecificAlveolar cells type 2
Function & Pathway
Relations & Evidence

Enzyme-Mediated Modification

5 records.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
VIL1SRCP12931Y81phosphorylationPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperKEA:15342783SIGNOR:15342783ProtMapper:15342783
VIL1SRCP12931Y256phosphorylationPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperKEA:15342783SIGNOR:15342783ProtMapper:15342783
VIL1SRCP12931Y60phosphorylationphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperKEA:15342783SIGNOR:15342783ProtMapper:15342783
VIL1SRCP12931Y64phosphorylationPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
VIL1SRCP12931Y46phosphorylationPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper

Ligand-Receptor Signaling

5 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularLOCATENoNoNoNoNo
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo

Regulatory Interaction Network

1 record.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
SRCP12931VILIP09327YesYesNophosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefPhosphoPointSIGNORProtMapperiPTMnetHPRDPhosphoSite_KEAKEAHPRD_KEAInnateDBSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperSIGNOR:15342783ProtMapper:15342783HPRD:15342783PhosphoSite:12269817InnateDB:17537734PhosphoSite:16921170KEA:15342783PhosphoSite:15342783

Protein Complex Composition

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
AVILVIL1O75366P093270:0hu.MAP2
VIL1P093272PDBPDB:3fg7

Isolation & Detection Technology

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationMass spectrometry23778691838321535
Sequence, Structure & Domains

Sequences

Length
827
Mass
92,695
Sequence
MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSSGPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQNLKKEKGLF
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P09327-1; Sequence=Displayed; Name=2; IsoId=P09327-2; Sequence=VSP_054436, VSP_054437
Alternative Sequence
368..421; AKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGHF -> GEGQAGAVREPGSRSWARRATWSTTHPPSLTCIFNEDFYAGSGLVLADGDVDKL (in isoform 2); 422..827; Missing (in isoform 2)

3D Structural Models

Helix
641..643; 668..684; 705..708; 795..800; 806..811; 814..823
Beta Strand
620..625; 632..635; 648..653; 658..662; 685..688; 695..699
3D Structure
NMR spectroscopy (1); X-ray crystallography (1)

Domain & Motif Annotations

Repeat
27..76; Gelsolin-like 1; 148..188; Gelsolin-like 2; 265..309; Gelsolin-like 3; 407..457; Gelsolin-like 4; 528..568; Gelsolin-like 5; 631..672; Gelsolin-like 6
Domain (CC)
Consists of a large core fragment in the N-terminal portion and a small headpiece (HP) in the C-terminal portion. The core fragment is necessary for both actin-nucleating and -severing activities, whereas the HP binds F-actin strongly in both the presence and absence of calcium and is necessary in actin-bundling activity. The Gelsolin-like 1 repeat is necessary for the actin-capping activity. The entire core fragment is necessary for the actin-severing activity. Two major calcium-sensitive sites are involved in conformational changes and determine separate functional properties: the first site (Glu-25, Asp-44 and Glu-74) regulates the actin-capping and actin-severing activities; while the second site (Asp-61, Asp-86 and Ala-93) regulates only the actin-severing activity.
Domain (FT)
761..827; HP
Region
2..734; Core; 2..126; Necessary for homodimerization; 112..119; LPA/PIP2-binding site 1; 138..146; LPA/PIP2-binding site 2; 735..827; Headpiece; 816..824; LPA/PIP2-binding site 3
Protein Families
Villin/gelsolin family
Sequence Similarities
Belongs to the villin/gelsolin family.
Clinical Relevance
Disease Involvement
Cancer-related genes