Protein detail
ARAF
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Entry name ARAF | UniProt ID | EVMP confidence score 0.50 |
Supporting publications (n) 8 | Transmembrane count | Protein classification EnzymesHuman disease related genesPlasma proteinsPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Protein Class (4)
EnzymesHuman disease related genesPlasma proteinsPredicted intracellular proteins
Protein Function (5)
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- Enzymes
- Human disease related genes:Cancers:Cancers of haematopoietic and lymphoid tissues
- Kinases:TKL Ser/Thr protein kinases
Ensembl
Entrez Gene Symbol
Gene Synonym
ARAF1
Gene Description
A-Raf proto-oncogene, serine/threonine kinase
Chromosome
X
Position
47561205-47571908
Supporting publications (n)
8
EVMP confidence score
0.50
Fluorescence & Localization1
Function & Pathway7
Protein Function (5)
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- Enzymes
- Human disease related genes:Cancers:Cancers of haematopoietic and lymphoid tissues
- Kinases:TKL Ser/Thr protein kinases
Cellular Component (3)
Molecular Function (8)
Biological Process (3)
KEGG (36)
- hsa01521 EGFR tyrosine kinase inhibitor resistance
- KEGG:hsa01522 Endocrine resistance
- KEGG:hsa04010 MAPK signaling pathway
- KEGG:hsa04012 ErbB signaling pathway
- KEGG:hsa04068 FoxO signaling pathway
- KEGG:hsa04270 Vascular smooth muscle contraction
- KEGG:hsa04650 Natural killer cell mediated cytotoxicity
- KEGG:hsa04720 Long-term potentiation
- KEGG:hsa04726 Serotonergic synapse
- KEGG:hsa04730 Long-term depression
Page 1 of 4
Reactome (10)
- R-hsa-5663202 diseases of signal transduction by growth factor receptors and second messengers
- R-hsa-5674135 map2k and mapk activation
- R-hsa-5684996 mapk1 mapk3 signaling
- R-hsa-5683057 mapk family signaling cascades
- R-hsa-5675221 negative regulation of mapk pathway
- R-hsa-6802957 oncogenic mapk signaling
- R-hsa-5673000 raf activation
- R-hsa-6802952 signaling by braf and raf1 fusions
- R-hsa-6802946 signaling by moderate kinase activity braf mutants
- R-hsa-9660537 signaling by mras complex mutants
Mediation Categories (2)
Clinical-translation mediationReceptor-signaling mediation
Relations & Evidence42
Enzyme-Mediated Modification (18)
18 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| ARAF | JAK2 | O60674 | Y | 302 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | JAK2 | O60674 | T | 455 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | JAK2 | O60674 | T | 452 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | JAK2 | O60674 | S | 299 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | BRAF | P15056 | Y | 302 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | BRAF | P15056 | T | 455 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | BRAF | P15056 | T | 452 | phosphorylation | Reactome_ProtMapperProtMapper | |
| ARAF | BRAF | P15056 | S | 299 | phosphorylation | Reactome_ProtMapperProtMapper |
Page 2 of 2Previous
Ligand-Receptor Signaling (6)
6 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
Regulatory Interaction Network (10)
10 records.
Protein Complex Composition (7)
7 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| ARAF-PIK3R1 complex | ARAFPIK3R1 | P10398P27986 | 0:0 | CORUM | CORUM:7361 | 10967104 |
| CAPN7CHMP5PIP4P1SARAFSPASTVPS4BVPS9D1VTA1 | O75351Q86T03Q96BY9Q9NP79Q9NZZ3Q9UBP0Q9Y2B5Q9Y6W3 | 0:0:0:0:0:0:0:0 | hu.MAP | |||
| CAPN7CHMP2BCHMP5SARAFVPS4BVTA1 | O75351Q96BY9Q9NP79Q9NZZ3Q9UQN3Q9Y6W3 | 0:0:0:0:0:0 | hu.MAP | |||
| APOA1ARAFKRAS | P01116P02647P10398 | 1:2:1 | PDB | PDB:2mse | ||
| ARAFSFN | P10398P31947 | 1:1 | PDB | PDB:8vsmPDB:8vslPDB:8vsn | ||
| ARL14EPFAF2SARAFSETDB1 | Q15047Q8N8R7Q96BY9Q96CS3 | 0:0:0:0 | hu.MAP | |||
| SARAF | Q96BY9 | 2 | PDB | PDB:6o2wPDB:6o2vPDB:6o2u |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography | Mass spectrometryMass spectrometry [LTQ-FT Ultra]Small R sequencing (Illumi HiSeq 2000 (Solexa) | 10 | 28211531331694213141437729441425289145002577684628240915317186093233126734202855 |
Sequence, Structure & Domains15
Sequences
Length
606
Mass
67,585
Sequence
MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P10398-1; Sequence=Displayed; Name=2; Synonyms=DA-Raf1; IsoId=P10398-2; Sequence=VSP_045609, VSP_045610
Alternative Sequence
186; S -> R (in isoform 2); 187..606; Missing (in isoform 2)
3D Structural Models
Turn
51..53
Helix
42..50; 79..81
Beta Strand
19..24; 26..28; 30..34; 56..67; 69..72; 74..78; 82..84; 85..90
3D Structure
NMR spectroscopy (2); X-ray crystallography (4)
Domain & Motif Annotations
Compositional Bias
274..289; Basic and acidic residues
Zinc Finger
98..144; Phorbol-ester/DAG-type
Domain (FT)
19..91; RBD; 310..570; Protein kinase
Region
160..207; Disordered; 240..290; Disordered
Protein Families (3)
- Protein kinase superfamily
- TKL Ser/Thr protein kinase family
- RAF subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Clinical Relevance6
Disease Involvement
Proto-oncogene
Drugs (55)
TRAMETINIB DIMETHYL SULFOXIDEL-372662ARGIPRESSIND(CH2)5[TYR(ME)2,THR4,TYR-NH29]OVTRO5028442D(CH2)5[TYR(ME)2,THR4,TYR(3125I)-NH29]OVTREGORAFENIBCOBIMETINIBL-366,948L-369,020D(CH2)5[TYR(ME)2]AVPSORAFENIBDESMOPRESSINTOVORAFENIBRETOSIBANXL281COMPOUND 37 [PMID: 16250654]SSR126768AENCORAFENIB[PHE3]OTL-368,930D(CH2)5[TYR(ME)2,THR4,PHE(3125I,4N3)-NH29]OVTDESGLYNH2-D(CH2)5[TYR(ME)2,THR4,ORN8]OTLIT-001[3H]AVP (HUMAN, MOUSE, RAT)BELVARAFENIBARGIPRESTOCINLS-192629[MPA1,D-TYR(ET)2,D-TIC7,AIB9]OTNELIVAPTANL-365,209L-366,682[MPA1,D-TYR(ET)2,D-TIC7]OTLY-3009120CARBETOCIN[THR4,GLY7]OTL023103[MPA1,D-TYR(ET)2,D-TIC7,D-TIC9]OT[125I]D(CH2)5[TYR(ME)2,THR4,ORN8,TYR-NH29]OVTRELCOVAPTANATOSIBANL-368,228BALOVAPTAND[CHA4]AVPL-368,899D(CH2)5[TYR(ME)2,THR4,PHE(3I,4N3)-NH29]OVTD[LEU4]AVPOPC-21268L-371,257[35S]NON-PEPTIDE OT ANTAGONISTL-367,773[111IN]DOTA-DLVTL-366,875L-366,509L-367,938
Interaction Protein (19)
ENSG00000096384ENSG00000101158ENSG00000105197ENSG00000105401ENSG00000108953ENSG00000126934ENSG00000128245ENSG00000132155ENSG00000134070ENSG00000134308ENSG00000150455ENSG00000157764ENSG00000164924ENSG00000166913ENSG00000169032ENSG00000170027ENSG00000174775ENSG00000175793ENSG00000185507
Interaction Count
19
Interaction Dataset (4)
intact_biogridbiogrid_opencell_bioplexintact_biogrid_opencellbiogrid_opencell
Supporting Publications8
| PMID | Title | Abstract |
|---|---|---|
| 27487081 | Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation. | No abstract available |
| 36064647 | Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells. | No abstract available |
| 36146834 | Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. | No abstract available |
| 36982312 | Saliva and Saliva Extracellular Vesicles for Biomarker Candidate Identification-Assay Development and Pilot Study in Amyotrophic Lateral Sclerosis. | No abstract available |
| 37322475 | Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration. | No abstract available |
| 38113368 | In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum. | No abstract available |
| 38207106 | Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity. | No abstract available |
| 40189497 | Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis. | No abstract available |