EV MemProt Atlas

Basic Information

Protein Names

T-cell-specific surface glycoprotein CD28 (TP44) (CD antigen CD28)

Protein Class

CD markersPredicted membrane proteins

Protein Function

CD markers

Transmembrane

153..179; Helical

Transmembrane Count

1

Ensembl

ENSG00000178562

Entrez Gene Symbol

CD28

Gene Description

CD28 molecule

Chromosome

2

Position

203706475-203738912

Frequency

2

EVMP Score

0.50

Fluorescence & Localization

Cell SpecificEnterocytesSingle-Nuclei Brain Specificcommitted oligodendrocyte precursorBlood Cell SpecificbasophilBlood Lineage SpecificgranulocytesSecretome LocationSecreted - unknown locationSecretome FunctionReceptor

Function & Pathway

Protein Function

CD markers

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Mediation Categories

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

12 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
CD28	ITK	Q08881	Y	209	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapper	SIGNOR:22936936 HPRD:15144186 phosphoELM:8992971 KEA:15144186 KEA:16094384 KEA:8992971 KEA:8946678 SIGNOR:8992971 KEA:15592455 ProtMapper:22936936 ProtMapper:8992971 HPRD:19901323 HPRD:8992971
CD28	ITK	Q08881	Y	218	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapper	HPRD:15144186 phosphoELM:8992971 KEA:15144186 KEA:8992971 KEA:8946678 ProtMapper:8992971 HPRD:8992971
CD28	ITK	Q08881	Y	191	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapper	SIGNOR:22936936 phosphoELM:8992971 KEA:16094384 KEA:8992971 SIGNOR:8992971 ProtMapper:22936936 ProtMapper:8992971 HPRD:19901323 HPRD:8992971
CD28	ITK	Q08881	Y	206	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	KEA:8992971 KEA:8946678 SIGNOR:8992971 ProtMapper:8992971 HPRD:8992971
CD28	LCK	P06239	Y	191	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperReactome_ProtMapperKEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapper	SIGNOR:22936936 phosphoELM:8992971 ProtMapper:27460989 KEA:16094384 KEA:8992971 SIGNOR:8992971 ProtMapper:22936936 ProtMapper:8992971 ProtMapper:7568038
CD28	LCK	P06239	Y	209	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:27460989
CD28	SRC	P12931	Y	191	phosphorylation	Li2012Reactome_ProtMapperProtMapper
CD28	TEC	P42680	Y	191	phosphorylation	Li2012
CD28	LYN	P07948	Y	191	phosphorylation	Reactome_ProtMapperProtMapper
CD28	FYN	P06241	Y	191	phosphorylation	Reactome_ProtMapperREACH_ProtMapperProtMapper	ProtMapper:7568038

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Ligand-Receptor Signaling

51 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane	transmembrane	LOCATE	No	No	No	Yes	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	Yes	No
transmembrane	transmembrane	OmniPath	No	No	No	Yes	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	Yes	No
plasma_membrane	plasma_membrane	Cellinker	No	No	No	Yes	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	Membranome	No	No	No	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	CSPA	No	No	No	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	OmniPath	No	No	No	Yes	No
cell_surface	cell_surface	Surfaceome	No	No	No	Yes	No

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Regulatory Interaction Network

10 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
CD28	P10747	GRB2	P62993	Yes	Yes	No	WangSIGNORELMHINTHPRDBioGRIDLi2012SPIKE_LCSPIKE	HPRD:7737275 HINT:8576157 ELM:27927989 BioGRID:8576157 HPRD:8576157 HINT:27927989 ELM:24098653 SIGNOR:24098653 SPIKE:9694876 HINT:9694876 SPIKE:21487479 HPRD:9694876 HINT:7737275 SPIKE_LC:18829987 HINT:24475931 BioGRID:9694876 HINT:24098653 SPIKE_LC:21487479 SPIKE_LC:9694876 SIGNOR:7737275 SPIKE:18829987 HINT:7584133
CD28	P10747	CBLB	Q13191	Yes	No	Yes	SignaLink3TCRcuration_SignaLink3	SignaLink3:12193687 SignaLink3:23109003
CD28	P10747	P85A	P27986	Yes	No	No	WangNetPathHPRDHINTSignaLink3BioGRIDIntActInnateDBSPIKE_LCSPIKETCRcuration_SignaLink3	BioGRID:8183372 HPRD:7737275 SignaLink3:17722974 SignaLink3:23109003 SPIKE:8146197 SignaLink3:8080844 IntAct:8183372 HINT:9417079 HINT:27927989 InnateDB:18641334 SPIKE_LC:8146197 IntAct:9417079 HINT:8146197 IntAct:7807015 InnateDB:11526404 HINT:7568038 BioGRID:8164687 IntAct:7568038 SignaLink3:16107703 HINT:24475931 BioGRID:7509360 HINT:18641334 SignaLink3:22315502 HINT:10820259 SignaLink3:15790353 IntAct:8146197 IntAct:10820259 HPRD:8621607 HINT:8183372 HINT:7957566 HINT:7807015 NetPath:8146197 IntAct:7584133 HINT:11526404 HINT:7584133
CD28	P10747	VAV	P15498	Yes	No	No	ACSN_SignaLink3SignaLink3TCRcuration_SignaLink3	SignaLink3:16713974 SignaLink3:26192618 SignaLink3:11907067 SignaLink3:10426994 SignaLink3:7523569 SignaLink3:23109003 SignaLink3:15197224 SignaLink3:22437870 SignaLink3:11034388 SignaLink3:15536084 SignaLink3:9359474 SignaLink3:1313950
CD28	P10747		Q96EV4	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:17722974 SignaLink3:22315502 SignaLink3:15790353 SignaLink3:23109003 SignaLink3:8080844 SignaLink3:16107703
CD28	P10747		Q6FHA6	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:23109003 SignaLink3:10820259
CD28	P10747	ITK	Q08881	Yes	No	No	HPRDHINTSignaLink3WangTCRcuration_SignaLink3	HPRD:10586033 HPRD:15144186 SignaLink3:19132916 SignaLink3:23109003 SignaLink3:15771581 SignaLink3:9312162 HINT:7524075 HINT:9317120 HPRD:8992971
ITK	Q08881	CD28	P10747	Yes	Yes	No	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDHINTPhosphoSite_KEAKEAphosphoELM_KEAHPRD_KEAphosphoELMSIGNOR_ProtMapperHPRD-phos	HPRD:15144186 phosphoELM:8992971 KEA:16094384 HPRD-phos:8992971 HPRD-phos:15144186 KEA:8992971 HINT:7524075 SIGNOR:8992971 HINT:9317120 SIGNOR:22936936 KEA:15144186 KEA:15592455 ProtMapper:8992971 HPRD-phos:19901323 HPRD:10586033 ProtMapper:15144186 ProtMapper:19901323 KEA:8946678 ProtMapper:22936936 HPRD:8992971
LCK	P06239	CD28	P10747	Yes	Yes	No	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDRLIMS-P_ProtMapperPhosphoSite_KEAKEAReactome_ProtMapperphosphoELM_KEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapper	SIGNOR:22936936 HPRD:10430626 phosphoELM:8992971 ProtMapper:27460989 KEA:16094384 KEA:8992971 SIGNOR:8992971 ProtMapper:22936936 ProtMapper:8992971 ProtMapper:7568038
CD28	P10747	PK3CG	P48736	Yes	Yes	No	HPRDWangSIGNOR	SIGNOR:18006698 HPRD:9915850

Protein Complex Composition

6 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
CD28-transactivation complex	CD28 NCL	P10747 P19338	1:1	CompleatCORUM	CORUM:92Compleat:HC3376	12324461
	CD28 GRAP2	O75791 P10747	2:2	PDB	PDB:5gjh
	CD28	P10747	2	PDB	PDB:7vu5PDB:8s6z
	CD28 PIK3R1	P10747 P27986	1:1	PDB	PDB:5gjiPDB:5aul
	CD28 GRB2	P10747 P62993	1:1	PDB	PDB:3wa4
	CD28 PTPN11	P10747 Q06124	1:1	PDB	PDB:7ppn

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Polymer Precipitation	Western blotting	1	38731868

Sequence, Structure & Domains

Sequences

Length

220

Mass

25,066

Sequence

MLRLLLALNLFPSIQVTGNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS

Alternative Products

Event=Alternative splicing; Named isoforms=7; Name=1; IsoId=P10747-1; Sequence=Displayed; Name=2; Synonyms=CD28-S2; IsoId=P10747-2; Sequence=VSP_002494; Name=3; Synonyms=CD28i; IsoId=P10747-3; Sequence=VSP_002495; Name=4; Synonyms=CD28-S1; IsoId=P10747-4; Sequence=VSP_002496; Name=5; IsoId=P10747-5; Sequence=VSP_002495, VSP_002497, VSP_002498; Name=6; Synonyms=CD28-S3; IsoId=P10747-6; Sequence=VSP_002495, VSP_002499; Name=7; IsoId=P10747-7; Sequence=VSP_047701

Alternative Sequence

1..17; MLRLLLALNLFPSIQVT -> MPCGLSALIMCPKGMVAVVVAVDDGDSQALA (in isoform 7); 19..137; Missing (in isoform 2); 40..137; CKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKG -> W (in isoform 4); 40..124; CKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLD -> Y (in isoform 3, isoform 5 and isoform 6); 138..139; KH -> EE (in isoform 5); 140..220; Missing (in isoform 5); 152..207; Missing (in isoform 6)

3D Structural Models

Helix

104..106; 153..182

Beta Strand

27..30; 35..43; 49..58; 64..74; 77..79; 81..83; 85..90; 92..101; 108..121; 123..125; 131..134

3D Structure

NMR spectroscopy (2); X-ray crystallography (8)

Domain & Motif Annotations

Domain (FT)

28..137; Ig-like V-type

Region

198..220; Disordered

Clinical Relevance

Disease Involvement

Disease variant

Related Diseases

Brain cancer Diabetes mellitus Immune system disease Lupus erythematosus Malignant haematopoietic neoplasm Mature B-cell lymphoma Necrotising fasciitis Osteosarcoma Ovarian cancer Prostate cancer Respiratory infection Sarcoma Solid tumour/cancer Stomach cancer Transplant rejection

Biomarker

Phase 1/2; Discontinued in Phase 1; Investigative; Phase 2; Phase 1; Phase 3; Approved

Drugs

BENZODEPA PHOSPHATIDYLINOSITIDE 3-KINASE INHIBITOR RETINOIC ACID AGENT MPA TGN-1412 INTERLEUKIN-12 GENE NULL LULIZUMAB PEGOL ALDESLEUKIN GUSPERIMUS TRIHYDROCHLORIDE 3-HYDROXYANTHRANILIC ACID GELDANAMYCIN LORATADINE BELATACEPT BISPECIFIC ANTIBODY TETRADECANOYLPHORBOL ACETATE

Antibody

HPA041233 HPA070003

Protein symbol CD28	UniProt ID P10747	EVMP score 0.50
Frequency 2	Transmembrane count 1	Protein classification CD markersPredicted membrane proteins