Protein detail
BRAF
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf) (p94) (v-Raf murine sarcoma viral oncogene homolog B1)
Entry name BRAF | UniProt ID | EVMP score 0.38 |
Frequency 1 | Transmembrane count | Protein classification Cancer-related genesDisease related genesEnzymesFDA approved drug targetsHuman disease related genesPredicted intracellular proteinsTransporters |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf) (p94) (v-Raf murine sarcoma viral oncogene homolog B1)
Protein Class
Cancer-related genesDisease related genesEnzymesFDA approved drug targetsHuman disease related genesPredicted intracellular proteinsTransporters
Protein Function
- Cancer-related genes:Mutational cancer driver genes
- Human disease related genes:Cancers:Cancers of the digestive system
- Human disease related genes:Cancers:Cancers of the lung and pleura
- Predicted intracellular proteins
- Human disease related genes:Cancers:Skin cancers
- ENZYME proteins:Transferases
- Cancer-related genes:Mutated cancer genes
- Human disease related genes:Cancers:Cancers of endocrine organs
- Enzymes
- Human disease related genes:Cancers:Cancers of haematopoietic and lymphoid tissues
- Kinases:TKL Ser/Thr protein kinases
- Transporters:Accessory Factors Involved in Transport
- Human disease related genes:Congenital malformations:Other congenital malformations
- Disease related genes
- FDA approved drug targets:Small molecule drugs
Ensembl
Entrez Gene Symbol
Gene Synonym
BRAF-1BRAF1
Gene Description
B-Raf proto-oncogene, serine/threonine kinase
Chromosome
7
Position
140719327-140924929
Frequency
1
EVMP Score
0.38
Fluorescence & Localization
Cell SpecificEarly spermatidsSingle-Nuclei Brain Specificvascular associated smooth muscle cell
Function & Pathway
Protein Function
- Cancer-related genes:Mutational cancer driver genes
- Human disease related genes:Cancers:Cancers of the digestive system
- Human disease related genes:Cancers:Cancers of the lung and pleura
- Predicted intracellular proteins
- Human disease related genes:Cancers:Skin cancers
- ENZYME proteins:Transferases
- Cancer-related genes:Mutated cancer genes
- Human disease related genes:Cancers:Cancers of endocrine organs
- Enzymes
- Human disease related genes:Cancers:Cancers of haematopoietic and lymphoid tissues
- Kinases:TKL Ser/Thr protein kinases
- Transporters:Accessory Factors Involved in Transport
- Human disease related genes:Congenital malformations:Other congenital malformations
- Disease related genes
- FDA approved drug targets:Small molecule drugs
Cellular Component
Molecular Function
- GO:0004672 protein kinase activity
- GO:0004674 protein serine/threonine kinase activity
- GO:0004708 MAP kinase kinase activity
- GO:0004709 MAP kinase kinase kinase activity
- GO:0005509 calcium ion binding
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0031434 mitogen-activated protein kinase kinase binding
- GO:0042802 identical protein binding
- GO:0044024 histone H2AS1 kinase activity
- GO:0044877 protein-containing complex binding
- GO:0097110 scaffold protein binding
- GO:0106310 protein serine kinase activity
Biological Process
KEGG
- hsa01521 EGFR tyrosine kinase inhibitor resistance
- KEGG:hsa01522 Endocrine resistance
- KEGG:hsa04010 MAPK signaling pathway
- KEGG:hsa04012 ErbB signaling pathway
- KEGG:hsa04015 Rap1 signaling pathway
- KEGG:hsa04024 cAMP signaling pathway
- KEGG:hsa04062 Chemokine signaling pathway
- KEGG:hsa04068 FoxO signaling pathway
- KEGG:hsa04150 mTOR signaling pathway
- KEGG:hsa04270 Vascular smooth muscle contraction
- KEGG:hsa04510 Focal adhesion
- KEGG:hsa04650 Natural killer cell mediated cytotoxicity
- KEGG:hsa04720 Long-term potentiation
- KEGG:hsa04722 Neurotrophin signaling pathway
- KEGG:hsa04726 Serotonergic synapse
- KEGG:hsa04730 Long-term depression
- KEGG:hsa04810 Regulation of actin cytoskeleton
- KEGG:hsa04910 Insulin signaling pathway
- KEGG:hsa04914 Progesterone-mediated oocyte maturation
- KEGG:hsa04928 Parathyroid hormone synthesis, secretion and action
- KEGG:hsa04934 Cushing syndrome
- KEGG:hsa05010 Alzheimer disease
- KEGG:hsa05022 Pathways of neurodegeneration - multiple diseases
- KEGG:hsa05034 Alcoholism
- KEGG:hsa05160 Hepatitis C
- KEGG:hsa05161 Hepatitis B
- KEGG:hsa05200 Pathways in cancer
- KEGG:hsa05205 Proteoglycans in cancer
- KEGG:hsa05208 Chemical carcinogenesis - reactive oxygen species
- KEGG:hsa05210 Colorectal cancer
- KEGG:hsa05211 Renal cell carcinoma
- KEGG:hsa05212 Pancreatic cancer
- KEGG:hsa05213 Endometrial cancer
- KEGG:hsa05214 Glioma
- KEGG:hsa05215 Prostate cancer
- KEGG:hsa05216 Thyroid cancer
- KEGG:hsa05218 Melanoma
- KEGG:hsa05219 Bladder cancer
- KEGG:hsa05220 Chronic myeloid leukemia
- KEGG:hsa05221 Acute myeloid leukemia
- KEGG:hsa05223 Non-small cell lung cancer
- KEGG:hsa05224 Breast cancer
- KEGG:hsa05225 Hepatocellular carcinoma
- KEGG:hsa05226 Gastric cancer
Reactome
- R-hsa-170984 arms mediated activation
- R-hsa-5663202 diseases of signal transduction by growth factor receptors and second messengers
- R-hsa-5674135 map2k and mapk activation
- R-hsa-5684996 mapk1 mapk3 signaling
- R-hsa-5683057 mapk family signaling cascades
- R-hsa-5674499 negative feedback regulation of mapk pathway
- R-hsa-5654726 negative regulation of fgfr1 signaling
- R-hsa-5654727 negative regulation of fgfr2 signaling
- R-hsa-5654732 negative regulation of fgfr3 signaling
- R-hsa-5654733 negative regulation of fgfr4 signaling
- R-hsa-5675221 negative regulation of mapk pathway
- R-hsa-6802957 oncogenic mapk signaling
- R-hsa-169893 prolonged erk activation events
- R-hsa-5673000 raf activation
- R-hsa-6802952 signaling by braf and raf1 fusions
- R-hsa-190236 signaling by fgfr
- R-hsa-5654736 signaling by fgfr1
- R-hsa-5654738 signaling by fgfr2
- R-hsa-5654741 signaling by fgfr3
- R-hsa-5654743 signaling by fgfr4
- R-hsa-6802946 signaling by moderate kinase activity braf mutants
- R-hsa-9660537 signaling by mras complex mutants
- R-hsa-166520 signaling by ntrks
- R-hsa-9006934 signaling by receptor tyrosine kinases
- R-hsa-187687 signalling to erks
- R-hsa-187706 signalling to p38 via rit and rin
- R-hsa-1295596 spry regulation of fgf signaling
Mediation Categories
Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
81 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| BRAF | SGK2 | Q9HBY8 | S | 365 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA | KEA:10869359KEA:11410590 |
| BRAF | SGK3 | Q96BR1 | S | 365 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA | KEA:10869359KEA:11410590 |
| BRAF | PRKX | P51817 | S | 429 | phosphorylation | MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP | |
| BRAF | PRKY | O43930 | S | 429 | phosphorylation | MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP | |
| BRAF | PIN1 | Q13526 | T | 753 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19933846 |
| BRAF | PIN1 | Q13526 | T | 401 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19933846 |
| BRAF | PIN1 | Q13526 | S | 151 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19933846 |
| BRAF | PIN1 | Q13526 | S | 750 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19933846 |
| BRAF | PIN1 | Q13526 | T | 599 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19933846 |
| BRAF | PIN1 | Q13526 | S | 602 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19933846 |
Ligand-Receptor Signaling
9 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
Regulatory Interaction Network
32 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| BRAF | P15056 | SL9A1 | P19634 | Yes | No | No | RLIMS-P_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper | PhosphoSite:25578862ProtMapper:27555478PhosphoSite:27555478 |
| PAK1 | Q13153 | BRAF | P15056 | Yes | Yes | No | PhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPKEGG-MEDICUSMIMPHPRD_MIMPiPTMnetProtMapperPhosphoSite_KEAKEAKinexus_KEAphosphoELM_KEAphosphoELMREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | KEA:10205168ProtMapper:25733387KEA:15710605PhosphoSite:33931501phosphoELM:15710605KEA:17192257 |
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Protein Complex Composition
8 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| BRAF-CNK1 complexnot RAS stimulated | BRAFCNKSR1 | P15056Q969H4 | 1:1 | CompleatCORUM | CORUM:5925Compleat:HC2925 | 15845549 |
| BAG2BAG3BRAFCCSDNAJC3HSPA1BHSPA8HSPD1PMPCAYWHAZ | O14618O95816O95817P0DMV9P10809P11142P15056P63104Q10713Q13217 | 1:1:1:2:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC7209 | ||
| BAG2BAG3BRAFDNAJC3DYNLT1HSPA1BHSPA8PMPCASOD1 | O95816O95817P00441P0DMV9P11142P15056P63172Q10713Q13217 | 1:1:1:2:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC5763 | ||
| BRAF | P15056 | 2 | PDB | PDB:4h58PDB:4xv2PDB:5jrqPDB:4pp7PDB:4kspPDB:3tv6PDB:4mnfPDB:3idpPDB:4e26PDB:4g9rPDB:5vykPDB:4ksqPDB:6n0qPDB:3priPDB:4ehgPDB:3c4cPDB:6xloPDB:5valPDB:6b8uPDB:5ct7PDB:2fb8PDB:8f7pPDB:3og7PDB:6p7gPDB:7k0vPDB:4xv1PDB:3prfPDB:3q4cPDB:5c9cPDB:7shvPDB:6n0pPDB:3tv4PDB:8qqgPDB:1uwhPDB:5itaPDB:4g9cPDB:4jvgPDB:5vamPDB:4cqePDB:3skcPDB:3ny5PDB:5hidPDB:4rzwPDB:4mbjPDB:5jt2PDB:8c7xPDB:4e4xPDB:4yhtPDB:5hiePDB:6uuoPDB:3ppkPDB:4wo5PDB:3ppjPDB:6nsqPDB:4xv3PDB:6cadPDB:6v34PDB:6v2uPDB:7p3vPDB:3ii5PDB:4rzvPDB:4r5yPDB:4fk3PDB:3d4qPDB:5cswPDB:4ehePDB:3psbPDB:4dbnPDB:5jsmPDB:8f7oPDB:5fd2PDB:4fc0PDB:8c7yPDB:1uwjPDB:3psdPDB:3q96 | ||
| BRAFMLST8MTORRAP1ARHEBRICTORRPS6KB1RPTORTSC2YWHAEYWHAZ | P15056P23443P42345P49815P62258P62834P63104Q15382Q6R327Q8N122Q9BVC4 | 1:1:1:1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC5539 | ||
| BRAFFOXO1HDAC4MARK3RHEBTSC1TSC2YWHAEYWHAGYWHAZ | P15056P27448P49815P56524P61981P62258P63104Q12778Q15382Q92574 | 1:1:1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC9621 | ||
| BRAFSFN | P15056P31947 | 1:1 | PDB | PDB:9f35PDB:8vsoPDB:9ew6 | ||
| BRAFYWHAZ | P15056P63104 | 2:2 | PDB | PDB:8vypPDB:8vysPDB:6u2hPDB:8vyqPDB:8vyrPDB:6xagPDB:8vywPDB:6q0kPDB:8vyoPDB:7mffPDB:8vyvPDB:8vyuPDB:7mfe |
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity CaptureMicrofluidics-Based Methods | Mass spectrometry | 32 | 3640649138938674397661583150850038225453398737264054596330382917387318682316151324393738258445992688484128242843305502873060870033709510406199952323027825820723339139413768636640098346283965113076053831320591379223004121688440311616400914553435521138564289 |
Sequence, Structure & Domains
Sequences
Length
766
Mass
84,437
Sequence
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
3D Structural Models
Turn
162..164; 187..189; 586..588; 598..600; 602..605; 655..658
Helix
43..68; 76..103; 177..186; 192..194; 214..217; 448..450; 454..456; 492..505; 537..542; 550..569; 579..581; 609..612; 614..619; 622..626; 635..651; 662..671; 678..680; 687..696; 701..703; 707..721
Beta Strand
156..161; 165..170; 195..199; 206..208; 221..226; 458..466; 469..484; 487..489; 516..520; 522..524; 526..530; 534..536; 543..545; 582..585; 589..592; 629..631; 683..685; 723..726
3D Structure
Electron microscopy (21); NMR spectroscopy (4); X-ray crystallography (102)
Domain & Motif Annotations
Compositional Bias
1..13; Gly residues; 314..341; Low complexity; 348..363; Basic and acidic residues; 423..447; Basic and acidic residues
Zinc Finger
234..280; Phorbol-ester/DAG-type
Domain (FT)
155..227; RBD; 457..717; Protein kinase
Region
1..38; Disordered; 308..454; Disordered
Protein Families
- Protein kinase superfamily
- TKL Ser/Thr protein kinase family
- RAF subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Clinical Relevance
Disease Involvement
Cancer-related genesCardiomyopathyDeafnessDisease variantEctodermal dysplasiaFDA approved drug targetsIntellectual disabilityProto-oncogene
Related Diseases
Biomarker
Approved; Phase 2
Drug Targets
FDA approved drug targets
Drugs
SELUMETINIBNVP-TAE 226RG-7256CETUXIMABNULLOSIMERTINIBALPELISIBDNA-DEPENDENT PROTEIN KINASE-TARGETING SIDNA DT01BRAFV600/PI3K INHIBITOR ASN003HSP90 INHIBITOR XL888MAP855ENZALUTAMIDERAF-265BINIMETINIBPAN-RAF INHIBITOR LY3009120GEDATOLISIBPEMBROLIZUMABBI-847325RABEPRAZOLERAF INHIBITOR BDTX-4933FICERAFUSP ALFABRAF(V600E) KINASE INHIBITOR ABM-1310PILARALISIBDASATINIB ANHYDROUSSORAFENIBPLX8394ATEZOLIZUMABPANITUMUMABCCT196969NAB-PACLITAXELMOCETINOSTATTIZATERKIBCFT1946SAPITINIBNIVOLUMABIRINOTECAN HYDROCHLORIDEABEMACICLIBMITOGEN-ACTIVATED PROTEIN KINASE KINASE INHIBITORPYRVINIUMXL281REGORAFENIBU0126REFAMETINIBCRIZOTINIBLENVATINIBTEMSIROLIMUSGEFITINIBAFATINIBYM-254890AKT INHIBITOR MK2206DACTOLISIBALECTINIBTRAMETINIB DIMETHYL SULFOXIDEOBATOCLAXTOVORAFENIBDABRAFENIBVEMURAFENIBCCT241161ENCORAFENIBCOBIMETINIBAZ628GDC-0879RIBOCICLIBKO-947BELVARAFENIBBMS-908662EVEROLIMUSTORIPALIMAB-TPZIERLOTINIBDOXORUBICIN HYDROCHLORIDEP53-HDM2 INTERACTION INHIBITOR MI-773HYDROXYCHLOROQUINENAVITOCLAXEXARAFENIBLXH254PLX-4720TAK-733VINBLASTINEAZD6482NVP-AEW541BI-3406CHLOROQUINELEUCOVORIN CALCIUMFLUOROURACILTEMUTERKIBPF-04217903SAPANISERTIBCAPECITABINEPANOBINOSTATERK INHIBITOR CC-90003RUCAPARIBE6201GANETESPIBBRAF(V600E) KINASE INHIBITOR RO5212054IMATINIBARQ-736LIFIRAFENIBZM336372MK-8353OXALIPLATINBI-2536PIMASERTIBGILTERITINIBAGERAFENIBRAVOXERTINIBNAPORAFENIBLAPATINIBSCH772984PONATINIBTUBASTATIN AP53/HDM2 INTERACTION INHIBITOR CGM097PICTILISIBROMIDEPSINCHIR-265MIRANSERTIBPLX-8394XL-281OMIPALISIBDOXORUBICIN LIPOSOMETUNLAMETINIBSB590885TW-37RINETERKIBCOMMENSAL BACTERIAL STRAIN FORMULATION VE800ZSTK474VTX-11EBUPARLISIBMIRDAMETINIBULIXERTINIBBOSUTINIBTGX-221SONOLISIBDEL-22379PALBOCICLIBSARACATINIBMEK INHIBITOR RO4987655SINTILIMABBRAF INHIBITORBEVACIZUMAB-AWWB(10R)-10-METHYL-3-(6-METHYLPYRIDIN-3-YL)-9,10,11,12-TETRAHYDRO-8H-[1,4]DIAZEPINO[5',6':4,5]THIENO[3,2-F]QUINOLIN-8-ONEMEK INHIBITOR IMM-1-104RUXOLITINIBASTX029VORUCICLIBIPILIMUMABMILADEMETANUPROSERTIBTIVOZANIBLY3009120VENETOCLAXLY-3009120PF-00477736ERK1/2 INHIBITOR ERAS-007TINLORAFENIBGEMCITABINEENTINOSTATAZD3514AXITINIBSY-5609LORLATINIBGSK-2636771LGX-806VORINOSTATPAC-1TALAZOPARIBIDELALISIBB-RAF/VEGFR-2 INHIBITOR RAF265CEDIRANIBERK1/2 INHIBITOR JSI-1187FUTUXIMAB/MODOTUXIMAB MIXTUREAVUTOMETINIBDENOSUMABRIFAMPINOLAPARIBNERATINIBPAN-RAF INHIBITOR XP-102CELECOXIBSHP2 INHIBITOR PF-07284892PI3K/BET INHIBITOR LY294002SAR 260301.00GSK2334470NAVTEMADLINCI-1040SPARTALIZUMABMEK 1/2 INHIBITOR FCN-159VALPROIC ACIDBRAF INHIBITOR BGB-3245TAK-632FUTIBATINIB
Interaction Protein
ENSG00000078061ENSG00000096384ENSG00000105401ENSG00000108953ENSG00000126934ENSG00000132155ENSG00000134308ENSG00000140575ENSG00000141564ENSG00000142208ENSG00000164924ENSG00000166913ENSG00000169032ENSG00000170027ENSG00000174775ENSG00000175793ENSG00000213281
Interaction Count
17
Interaction Dataset
intact_biogridbiogrid_bioplex