Protein detail
ITB4
Integrin beta-4 (GP150) (CD antigen CD104)
Entry name ITB4 | UniProt ID | EVMP confidence score 0.50 |
Supporting publications (n) 4 | Transmembrane count 1 | Protein classification Cancer-related genesCD markersDisease related genesHuman disease related genesPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information13
Protein Names
Integrin beta-4 (GP150) (CD antigen CD104)
Protein Class (7)
Cancer-related genesCD markersDisease related genesHuman disease related genesPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins
Protein Function (5)
- Predicted intracellular proteins
- CD markers
- Cancer-related genes:Candidate cancer biomarkers
- Human disease related genes:Congenital malformations:Congenital malformations of skin
- Disease related genes
Transmembrane
711..733; Helical
Transmembrane Count
1
Ensembl
Entrez Gene Symbol
Gene Synonym
CD104
Gene Description
Integrin subunit beta 4
Chromosome
17
Position
75721328-75757818
Supporting publications (n)
4
EVMP confidence score
0.50
Fluorescence & Localization6
Tissue SpecificovaryBrain Regional Specificchoroid plexusCell SpecificAdipocytesSingle-Nuclei Brain Specificchoroid plexus epithelial cellSecretome LocationSecreted to extracellular matrixSecretome FunctionCell adhesion
Function & Pathway7
Protein Function (5)
- Predicted intracellular proteins
- CD markers
- Cancer-related genes:Candidate cancer biomarkers
- Human disease related genes:Congenital malformations:Congenital malformations of skin
- Disease related genes
Cellular Component (13)
- GO:0005604 basement membrane
- GO:0005730 nucleolus
- GO:0005886 plasma membrane
- GO:0005925 focal adhesion
- GO:0008305 integrin complex
- GO:0009925 basal plasma membrane
- GO:0009986 cell surface
- GO:0030054 cell junction
- GO:0030056 hemidesmosome
- GO:0031252 cell leading edge
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Molecular Function (6)
Biological Process (3)
KEGG (11)
- hsa04151 PI3K-Akt signaling pathway
- KEGG:hsa04382 Cornified envelope formation
- KEGG:hsa04510 Focal adhesion
- KEGG:hsa04512 ECM-receptor interaction
- KEGG:hsa04518 Integrin signaling
- KEGG:hsa04810 Regulation of actin cytoskeleton
- KEGG:hsa04820 Cytoskeleton in muscle cells
- KEGG:hsa05165 Human papillomavirus infection
- KEGG:hsa05410 Hypertrophic cardiomyopathy
- KEGG:hsa05412 Arrhythmogenic right ventricular cardiomyopathy
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Reactome (12)
- R-hsa-2022090 assembly of collagen fibrils and other multimeric structures
- R-hsa-1500931 cell cell communication
- R-hsa-446728 cell junction organization
- R-hsa-1474290 collagen formation
- R-hsa-9734767 developmental cell lineages
- R-hsa-9734779 developmental cell lineages of the integumentary system
- R-hsa-9725554 differentiation of keratinocytes in interfollicular epidermis in mammalian skin
- R-hsa-1474244 extracellular matrix organization
- R-hsa-3000157 laminin interactions
- R-hsa-3000171 non integrin membrane ecm interactions
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Mediation Categories (2)
Adhesion and uptake mediationReceptor-signaling mediation
Relations & Evidence88
Enzyme-Mediated Modification (13)
13 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| ITGB4 | PRKCA | P17252 | S | 1,364 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperKEAphosphoELMPhosphoSitePhosphoSite_ProtMapper | phosphoELM:15121854KEA:15121854 |
| ITGB4 | PRKCA | P17252 | S | 1,356 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperKEAphosphoELMPhosphoSitePhosphoSite_ProtMapper | phosphoELM:15121854KEA:15121854 |
| ITGB4 | PRKCA | P17252 | S | 1,360 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:15121854KEA:15121854SIGNOR:15121854phosphoELM:15121854 |
| ITGB4 | PRKCA | P17252 | S | 1,494 | phosphorylation | PhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:15121854SIGNOR:15121854 |
| ITGB4 | PRKCA | P17252 | S | 1,424 | phosphorylation | PhosphoSite | |
| ITGB4 | PRKACA | P17612 | S | 1,364 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:17615294ProtMapper:17615294 |
| ITGB4 | MAPK3 | P27361 | S | 1,356 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| ITGB4 | PRKD1 | Q15139 | T | 1,736 | phosphorylation | PhosphoSite | |
| ITGB4 | PRKD2 | Q9BZL6 | T | 1,736 | phosphorylation | PhosphoSite | |
| ITGB4 | RPS6KA1 | Q15418 | S | 1,364 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper |
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Ligand-Receptor Signaling (59)
59 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| adhesion | adhesion | MCAM | Yes | Yes | No | Yes | No |
| adhesion | adhesion | Adhesome | Yes | Yes | No | Yes | No |
| cell_adhesion | cell_adhesion | Cellinker | Yes | Yes | No | Yes | No |
| integrin | matrix_adhesion | Integrins | No | Yes | No | Yes | No |
| integrin | matrix_adhesion | UniProt_keyword | No | Yes | No | Yes | No |
| matrix_adhesion | matrix_adhesion | Zhong2015 | No | Yes | No | Yes | No |
| integrin | matrix_adhesion | Almen2009 | No | Yes | No | Yes | No |
| integrin | matrix_adhesion | OmniPath | No | Yes | No | Yes | No |
| adhesion | adhesion | OmniPath | Yes | Yes | No | Yes | No |
| cell_adhesion | cell_adhesion | OmniPath | Yes | Yes | No | Yes | No |
Regulatory Interaction Network (13)
13 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| ITB4 | P16144 | PK3CB | P42338 | Yes | Yes | No | NetPathSIGNORCui2007CancerCellMapWang | CancerCellMap:11733063SIGNOR:9428518NetPath:11733063CancerCellMap:8143784CancerCellMap:7721947CancerCellMap:12867433 |
| FGFR1 | P11362 | ITB4 | P16144 | Yes | No | No | Sparser_ProtMapperiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:23677256PhosphoSite:11438664ProtMapper:26918348 |
| KPCD1 | Q15139 | ITB4 | P16144 | Yes | Yes | No | WangiPTMnetPhosphoSite | PhosphoSite:22357621PhosphoSite:26580203 |
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Protein Complex Composition (2)
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Protein Organic Solvent Precipitation | Mass spectrometry | 1 | 32384937 |
Sequence, Structure & Domains15
Sequences
Length
1,822
Mass
202,167
Sequence
MAGPRPSPWARLLLAALISVSLSGTLANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSNIVELLEEAFNRIRSNLDIRALDSPRGLRTEVTSKMFQKTRTGSFHIRRGEVGIYQVQLRALEHVDGTHVCQLPEDQKGNIHLKPSFSDGLKMDAGIICDVCTCELQKEVRSARCSFNGDFVCGQCVCSEGWSGQTCNCSTGSLSDIQPCLREGEDKPCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGPSCDCPLSNATCIDSNGGICNGRGHCECGRCHCHQQSLYTDTICEINYSAIHPGLCEDLRSCVQCQAWGTGEKKGRTCEECNFKVKMVDELKRAEEVVVRCSFRDEDDDCTYSYTMEGDGAPGPNSTVLVHKKKDCPPGSFWWLIPLLLLLLPLLALLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDVVRWKVTNNMQRPGFATHAASINPTELVPYGLSLRLARLCTENLLKPDTRECAQLRQEVEENLNEVYRQISGVHKLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKPALLKLTEKQVEQRAFHDLKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPAGTATLGRRLVNITIIKEQARDVVSFEQPEFSVSRGDQVARIPVIRRVLDGGKSQVSYRTQDGTAQGNRDYIPVEGELLFQPGEAWKELQVKLLELQEVDSLLRGRQVRRFHVQLSNPKFGAHLGQPHSTTIIIRDPDELDRSFTSQMLSSQPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNDDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSSDAEAPHGPPDDGGAGGKGGSLPRSATPGPPGEHLVNGRMDFAFPGSTNSLHRMTTTSAAAYGTHLSPHVPHRVLSTSSTLTRDYNSLTRSEHSHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEFVSRTLTTSGTLSTHMDQQFFQT
Alternative Products
Event=Alternative splicing; Named isoforms=5; Name=Beta-4C; IsoId=P16144-1; Sequence=Displayed; Name=Beta-4A; IsoId=P16144-2; Sequence=VSP_002749; Name=Beta-4B; IsoId=P16144-3; Sequence=VSP_002749, VSP_002750; Name=Beta-4D; IsoId=P16144-4; Sequence=VSP_002749, VSP_002751; Name=Beta-4E; IsoId=P16144-5; Sequence=VSP_002747, VSP_002748
Alternative Sequence
851..964; LNEVYRQISGVHKLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKPALLKLTEKQVEQRAFHDLKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEA -> VRTQELGLAGDVAERGLQADLRCTQAPADQVPAAAQCREKARPHHCGHSADGAPLGQAGPAEAYREAGGTEGLPRPQGGPRLLHPHCRPGRPGHGGVPGGRGAGGRTGAPLYPA (in isoform Beta-4E); 965..1822; Missing (in isoform Beta-4E); 1370..1439; Missing (in isoform Beta-4A, isoform Beta-4B and isoform Beta-4D); 1519; H -> HGLPPIWEHGRSRLPLSWALGSRSRAQMKGFPPSRGPRDSIILAGRPAAPSWGP (in isoform Beta-4B); 1678..1685; CEMAQGGG -> W (in isoform Beta-4D)
3D Structural Models
Turn
1035..1037; 1070..1073; 1567..1569; 1632..1635
Helix
1003..1005; 1168..1170; 1316..1318
Beta Strand
990..995; 997..1002; 1006..1016; 1022..1033; 1043..1048; 1054..1061; 1076..1087; 1096..1103; 1131..1137; 1139..1141; 1143..1148; 1156..1163; 1172..1183; 1191..1200; 1203..1207; 1211..1214; 1227..1230; 1232..1234; 1236..1239; 1252..1260; 1262..1264; 1266..1268; 1271..1275; 1282..1286; 1294..1302; 1310..1314; 1334..1336; 1344..1348; 1522..1524; 1532..1540; 1543..1549; 1558..1566; 1573..1577; 1584..1587; 1595..1604; 1612..1620; 1636..1639; 1648..1653; 1656..1662; 1671..1680; 1683..1685; 1688..1694; 1697..1703; 1712..1722; 1724..1732
3D Structure
NMR spectroscopy (1); X-ray crystallography (12)
Domain & Motif Annotations
Compositional Bias
1418..1427; Gly residues; 1503..1518; Polar residues
Domain (CC)
The VWFA domain (or beta I domain) contains three cation-binding sites: the ligand-associated metal ion-binding site (LIMBS or SyMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent MIDAS site (ADMIDAS). This domain is also part of the ligand-binding site.; DOMAIN: The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
Domain (FT)
29..73; PSI; 131..329; VWFA; 457..491; I-EGF 1; 492..537; I-EGF 2; 538..574; I-EGF 3; 575..615; I-EGF 4; 979..1084; Calx-beta; 1129..1218; Fibronectin type-III 1; 1222..1321; Fibronectin type-III 2; 1530..1625; Fibronectin type-III 3; 1643..1739; Fibronectin type-III 4
Region
194..199; Involved in NRG1- and IGF1-binding; 732..749; Palmitoylated on several cysteines; 1113..1140; Disordered; 1400..1444; Disordered; 1451..1752; Interaction with ARHGEF40; 1495..1525; Disordered
Protein Families
Integrin beta chain family
Sequence Similarities
Belongs to the integrin beta chain family.
Clinical Relevance2
Supporting Publications4
| PMID | Title | Abstract |
|---|---|---|
| 24505114 | Proteomics analysis of cancer exosomes using a novel modified aptamer-based array (SOMAscan™) platform. | These included proteins of known association with cancer exosomes such as MFG-E8, integrins, and MET, and also those less widely reported as exosomally associated, such as ROR1 and ITIH4. |
| 31588238 | Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment. | No abstract available |
| 37922300 | Proteomic profiling of urinary extracellular vesicles differentiates breast cancer patients from healthy women. | No abstract available |
| 38731868 | The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer. | No abstract available |