Protein detail

PECA1

Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)

Entry name PECA1	UniProt ID P16284	EVMP confidence score 0.38
Supporting publications (n) 3	Transmembrane count 1	Protein classification Cancer-related genesCandidate cardiovascular disease genesCD markersPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)

Protein Class (6)

Cancer-related genesCandidate cardiovascular disease genesCD markersPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (4)

Candidate cardiovascular disease genes
Cancer-related genes:Candidate cancer biomarkers
CD markers
Predicted intracellular proteins

Transmembrane

602..620; Helical

Transmembrane Count

Ensembl

ENSG00000261371

Entrez Gene Symbol

PECAM1

Gene Synonym

CD31

Gene Description

Platelet and endothelial cell adhesion molecule 1

Chromosome

Position

64319415-64413776

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Tissue Specificblood vesselCell SpecificEndometrial stromal cellsSingle-Nuclei Brain Specificendothelial cellBlood Cell Specificmemory B-cell

Function & Pathway7

Protein Function (4)

Candidate cardiovascular disease genes
Cancer-related genes:Candidate cancer biomarkers
CD markers
Predicted intracellular proteins

Cellular Component (9)

Molecular Function (3)

Biological Process (3)

KEGG (5)

Reactome (14)

Page 1 of 2

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationImmune mediation

Relations & Evidence84

Enzyme-Mediated Modification (17)

17 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PECAM1	FYN	P06241	Y	690	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
PECAM1	FYN	P06241	Y	713	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
PECAM1	FYN	P06241	Y	663	phosphorylation	Reactome_ProtMapperProtMapper
PECAM1	LYN	P07948	Y	663	phosphorylation	Reactome_ProtMapperProtMapper
PECAM1	YES1	P07947	Y	663	phosphorylation	Reactome_ProtMapperProtMapper
PECAM1	SRC	P12931	Y	663	phosphorylation	Reactome_ProtMapperProtMapper
PECAM1	HCK	P08631	Y	713	phosphorylation	KEA	KEA:17570479

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Ligand-Receptor Signaling (59)

59 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ligand	ligand	OmniPath	Yes	No	No	Yes	No
receptor	receptor	talklr	No	Yes	No	Yes	No
receptor	receptor	connectomeDB2020	No	Yes	No	Yes	No
receptor	receptor	iTALK	No	Yes	No	Yes	No
receptor	receptor	EMBRACE	No	Yes	No	Yes	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	Yes	No
transmembrane_phobius	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_sosui	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	Yes	No

Page 6 of 6Previous

Regulatory Interaction Network (6)

6 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PECA1	P16284	CD38	P28907	Yes	Yes	No	CellChatDBCellPhoneDBICELLNETSIGNORconnectomeDB2020HPRDCellPhoneDB_CellinkerBaccin2019CellinkerWangLRdb	ICELLNET:26407101 CellChatDB:26407101 ICELLNET:9551996 connectomeDB2020:26407101 SIGNOR:18626062 Baccin2019:23308177 HPRD:9551996 Cellinker:9551996 LRdb:9551996 connectomeDB2020:9551996
LCK	P06239	PECA1	P16284	Yes	Yes	No	iPTMnetPhosphoPointSIGNORProtMapperHPRDReactome_ProtMapperKEAphosphoELM_KEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:9624175 HPRD:9624175 PhosphoSite:12207897 PhosphoSite:26956486 SIGNOR:9624175 ProtMapper:9624175
FER	P16591	PECA1	P16284	Yes	Yes	No	Sparser_ProtMapperiPTMnetSIGNORProtMapperdbPTMKEAphosphoELM_KEASIGNOR_ProtMapper	KEA:16731527 SIGNOR:12972546 dbPTM:9298995 KEA:9624175 dbPTM:19690332 ProtMapper:12972546 dbPTM:18083107 dbPTM:15592455 ProtMapper:33117780 dbPTM:19342684 dbPTM:18088087
CSK	P41240	PECA1	P16284	Yes	Yes	No	iPTMnetPhosphoPointSIGNORProtMapperHPRDSIGNOR_ProtMapper	HPRD:9624175 ProtMapper:9624175 SIGNOR:9624175
FES	P07332	PECA1	P16284	Yes	Yes	No	iPTMnetSIGNORProtMapperKEAphosphoELM_KEASIGNOR_ProtMapper	KEA:16731527 SIGNOR:12972546 ProtMapper:12972546 KEA:9624175
FYN	P06241	PECA1	P16284	Yes	No	No	iPTMnetPhosphoPointProtMapperHPRDReactome_ProtMapperSPIKE_LCPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:18182571 PhosphoSite:12535670 PhosphoSite:11927609 HPRD:10858437 SPIKE_LC:16713569 PhosphoSite:28974577 PhosphoSite:9312087 PhosphoSite:15201144

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	PECAM1	P16284	2	PDB	PDB:5gniPDB:5c14

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	30915084

Sequence, Structure & Domains14

Sequences

Length

738

Mass

82,522

Sequence

MQPRWAQGATMWLGVLLTLLLCSSLEGQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

Alternative Products

Event=Alternative splicing; Named isoforms=6; Name=Long; IsoId=P16284-1; Sequence=Displayed; Name=Delta12; IsoId=P16284-2; Sequence=VSP_011806; Name=Delta13; IsoId=P16284-3; Sequence=VSP_011807; Name=Delta14; IsoId=P16284-4; Sequence=VSP_011809; Name=Delta14-15; IsoId=P16284-5; Sequence=VSP_011808, VSP_011811; Name=Delta15; IsoId=P16284-6; Sequence=VSP_011810, VSP_011811

Alternative Sequence

664..681; Missing (in isoform Delta12); 682..702; Missing (in isoform Delta13); 703..729; Missing (in isoform Delta14-15); 703..721; Missing (in isoform Delta14); 722..729; Missing (in isoform Delta15); 730..738; RTEGSLDGT -> ENGRLP (in isoform Delta14-15 and isoform Delta15)

3D Structural Models

Turn

170..173

Helix

101..103; 709..728

Beta Strand

32..43; 45..48; 53..64; 71..77; 80..98; 105..115; 117..119; 123..128; 134..138; 140..142; 147..153; 161..169; 174..182; 184..194; 200..218; 222..227; 731..733

3D Structure

NMR spectroscopy (1); X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

686..698; Polar residues; 699..715; Basic and acidic residues

Motif

688..693; ITIM motif 1; 711..716; ITIM motif 2

Domain (CC)

The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.

Domain (FT)

35..121; Ig-like C2-type 1; 145..233; Ig-like C2-type 2; 236..315; Ig-like C2-type 3; 328..401; Ig-like C2-type 4; 424..493; Ig-like C2-type 5; 499..591; Ig-like C2-type 6

Region

658..715; Disordered; 709..729; Membrane-bound segment which detaches upon phosphorylation; 721..738; May play a role in cytoprotective signaling

Clinical Relevance6

Disease Involvement

Cancer-related genes

Drugs

RESVERATROL

Antibody (2)

HPA004690 HPA004871

Interaction Protein

ENSG00000197122

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications3

PMID	Title	Abstract
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available