Protein detail
AKAP5
A-kinase anchor protein 5 (AKAP-5) (A-kinase anchor protein 79 kDa) (AKAP 79) (H21) (cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein)
Protein symbol AKAP5 | UniProt ID | EVMP score 0.38 |
Frequency 1 | Transmembrane count | Protein classification Plasma proteinsPredicted intracellular proteinsTransporters |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
A-kinase anchor protein 5 (AKAP-5) (A-kinase anchor protein 79 kDa) (AKAP 79) (H21) (cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein)
Protein Class
Plasma proteinsPredicted intracellular proteinsTransporters
Protein Function
- Predicted intracellular proteins
- Transporters:Accessory Factors Involved in Transport
Ensembl
Entrez Gene Symbol
Gene Synonym
AKAP75AKAP79
Gene Description
A-kinase anchoring protein 5
Chromosome
14
Position
64465499-64474503
Frequency
1
EVMP Score
0.38
Fluorescence & Localization
Tissue SpecificliverCell SpecificEpicardial cellsSingle-Nuclei Brain Specificfibroblast
Function & Pathway
Protein Function
- Predicted intracellular proteins
- Transporters:Accessory Factors Involved in Transport
Cellular Component
- GO:0005829 cytosol
- GO:0005886 plasma membrane
- GO:0008287 protein serine/threonine phosphatase complex
- GO:0009898 cytoplasmic side of plasma membrane
- GO:0014069 postsynaptic density
- GO:0030425 dendrite
- GO:0032590 dendrite membrane
- GO:0043197 dendritic spine
- GO:0045121 membrane raft
- GO:0045211 postsynaptic membrane
- GO:0060076 excitatory synapse
- GO:0098837 postsynaptic recycling endosome
- GO:0098944 postsynaptic recycling endosome membrane
Molecular Function
- GO:0005515 protein binding
- GO:0005516 calmodulin binding
- GO:0008179 adenylate cyclase binding
- GO:0017124 SH3 domain binding
- GO:0030346 protein phosphatase 2B binding
- GO:0031698 beta-2 adrenergic receptor binding
- GO:0034237 protein kinase A regulatory subunit binding
- GO:0035254 glutamate receptor binding
- GO:0050811 GABA receptor binding
- GO:0051018 protein kinase A binding
- GO:0060090 molecular adaptor activity
- GO:0097110 scaffold protein binding
Biological Process
Reactome
- R-hsa-381676 glucagon like peptide 1 glp1 regulates insulin secretion
- R-hsa-163685 integration of energy metabolism
- R-hsa-9675108 nervous system development
- R-hsa-112316 neuronal system
- R-hsa-112314 neurotransmitter receptors and postsynaptic signal transmission
- R-hsa-422356 regulation of insulin secretion
- R-hsa-9010642 robo receptors bind akap5
- R-hsa-376176 signaling by robo receptors
- R-hsa-399719 trafficking of ampa receptors
- R-hsa-112315 transmission across chemical synapses
Mediation Categories
Fusion and delivery mediationMetabolism mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
4 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| AKAP5 | ZDHHC2 | Q9UIJ5 | C | 36 | palmitoylation | SIGNOR | SIGNOR:25589740 |
| AKAP5 | ZDHHC2 | Q9UIJ5 | C | 129 | palmitoylation | SIGNOR | SIGNOR:25589740 |
| AKAP5 | CAMK2A | Q9UQM7 | S | 92 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper | |
| AKAP5 | CAMK2A | Q9UQM7 | T | 87 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper |
Ligand-Receptor Signaling
4 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
Regulatory Interaction Network
3 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| AKAP5 | P24588 | KAPCA | P17612 | Yes | Yes | No | SIGNORCui2007HINTCA1Wang | CA1:8599116HINT:35271311CA1:1512224HINT:32707033HINT:26496610SIGNOR:10939335 |
| ZDHC2 | Q9UIJ5 | AKAP5 | P24588 | Yes | Yes | No | SIGNOR | SIGNOR:25589740 |
| AKAP5 | P24588 | PP2BC | P48454 | Yes | Yes | No | SIGNOR | SIGNOR:20694001 |
Protein Complex Composition
4 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| AKAP5-PRKAR2A-PPP3CA complex | AKAP5PPP3CAPRKAR2A | P13861P24588Q08209 | 1:1:1 | CompleatCORUM | Compleat:HC884CORUM:517 | 12507994 |
| PKA (RII-alpha and RII-beta)-AKAP5-ADRB1 complex | ADRB1AKAP5PRKAR2APRKAR2B | P08588P13861P24588P31323 | 0:0:0:0 | CORUM | CORUM:878 | 16940053 |
| PRKAC-AKAP5-ADRB1 complex | ADRB1AKAP5PRKACAPRKACBPRKACG | P08588P17612P22612P22694P24588 | 1:1:1:1:1 | Compleat | Compleat:HC1017 | 16940053 |
| AKAP5CALM1 | P0DP23P24588 | 2:2 | PDB | PDB:5nin |
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Mass spectrometry | 1 | 36435201 |
Sequence, Structure & Domains
Sequences
Length
427
Mass
47,088
Sequence
METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEASNSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAIIITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSLVKNAIQLSIEQLVNEMASDDNKINNLLQ
3D Structural Models
Turn
409..411
Helix
79..83; 393..408
Beta Strand
339..344
3D Structure
NMR spectroscopy (1); X-ray crystallography (2)
Domain & Motif Annotations
Compositional Bias
8..19; Basic and acidic residues; 37..48; Basic residues; 92..102; Basic and acidic residues; 171..182; Polar residues; 242..252; Polar residues; 302..333; Basic and acidic residues
Motif
76..96; AKAP CaM-binding
Domain (CC)
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.; DOMAIN: The N-terminal region, which is highly basic, is required for interaction with calmodulin.
Region
1..170; Essential to the intracellular anchoring function; 1..122; Disordered; 171..205; Disordered; 239..269; Disordered; 281..333; Disordered; 392..405; PKA-RII subunit binding domain; 410..427; Tethers NFATC2 to CRAC channels