Protein detail

MAP4

Microtubule-associated protein 4 (MAP-4)

Entry name MAP4	UniProt ID P27816	EVMP confidence score 0.75
Supporting publications (n) 21	Transmembrane count	Protein classification FDA approved drug targetsPlasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Microtubule-associated protein 4 (MAP-4)

Protein Class (3)

FDA approved drug targetsPlasma proteinsPredicted intracellular proteins

Protein Function (2)

FDA approved drug targets:Small molecule drugs
Predicted intracellular proteins

Ensembl

ENSG00000047849

Entrez Gene Symbol

MAP4

Gene Description

Microtubule associated protein 4

Chromosome

Position

47850690-48089272

Supporting publications (n)

EVMP confidence score

0.75

Fluorescence & Localization1

Function & Pathway5

Protein Function (2)

FDA approved drug targets:Small molecule drugs
Predicted intracellular proteins

Cellular Component (10)

Molecular Function (5)

Biological Process (3)

Mediation Categories

Clinical-translation mediation

Relations & Evidence58

Enzyme-Mediated Modification (33)

33 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
MAP4	MAPK9	P45984	S	787	phosphorylation	KEA	KEA:17570479
MAP4	PRKCB	P05771	S	941	phosphorylation	KEA	KEA:17570479
MAP4	PRKDC	P78527	S	1,073	phosphorylation	KEA	KEA:17570479

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Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MARK1	Q9P0L2	MAP4	P27816	Yes	No	Yes	SPIKEphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phosSPIKE_LC	HPRD-phos:19415658 HPRD-phos:18669648 ProtMapper:19415658 ProtMapper:18707149 SPIKE:8631898 HPRD-phos:18767875 SPIKE_LC:8631898 SPIKE:10542369 HPRD:8631898 KEA:17081983 HPRD-phos:20068231 ProtMapper:8631898 ProtMapper:17693683 ProtMapper:18212344 HPRD-phos:18452278 ProtMapper:18767875 HPRD-phos:20058876 ProtMapper:20058876 ProtMapper:19651622 HPRD-phos:18212344 SIGNOR:8631898 HPRD-phos:18707149 HPRD-phos:8631898 ProtMapper:20166139 ProtMapper:20068231 ProtMapper:18669648 ProtMapper:18452278 HPRD-phos:19651622 KEA:8631898 HPRD-phos:17693683 SPIKE_LC:10542369 HPRD-phos:20166139
CHK1	O14757	MAP4	P27816	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperPhosphoSite_ProtMapper	SIGNOR:36991467
CDK1	P06493	MAP4	P27816	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAHPRDWangPhosphoSite_ProtMapperNetworKIN_KEAphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteHPRD-phos	ProtMapper:10791892 HPRD-phos:19415658 HPRD:11683421 phosphoELM:9398320 KEA:11683421 ProtMapper:19415658 HPRD-phos:19691289 HPRD-phos:9398320 KEA:15216889 SIGNOR:9398320 HPRD-phos:20068231 HPRD:9398320 ProtMapper:11683421 PhosphoSite:10791892 KEA:9398320 ProtMapper:9398320 ProtMapper:19691289 ProtMapper:20068231 HPRD-phos:11683421 SIGNOR:10791892 KEA:17570479 ProtMapper:19664995 HPRD-phos:19664995
TTK	P33981	MAP4	P27816	Yes	No	Yes	PhosphoSite_norefSIGNORProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:31253867 PhosphoSite:31253867
MK14	Q16539	MAP4	P27816	Yes	No	No	PhosphoSite_ProtMapperPhosphoSite_norefProtMapperKEAPhosphoSiteNetworKIN_KEA	KEA:17570479 PhosphoSite:9398320 PhosphoSite:25746230

Protein Complex Composition (14)

14 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
IL13RA1 MAP4K3	P78552 Q8IVH8	0:0	hu.MAP2
MAP3K11 MAP4K2 TRAF2	Q12851 Q12933 Q16584	1:1:1	CompleatCFinder	Compleat:HC8575
EIF2A MAP4K3	Q8IVH8 Q9BY44	0:0	hu.MAP
MAP4K1	Q92918	2	PDB	PDB:7siuPDB:9n9xPDB:7m0kPDB:9nc2PDB:8pasPDB:8fkoPDB:7r9pPDB:9cztPDB:9nacPDB:9nbsPDB:6cqePDB:7m0mPDB:7m0lPDB:7l26PDB:9bi8PDB:6nfzPDB:9czxPDB:8pauPDB:8cdwPDB:8fjzPDB:8xn7PDB:9n7rPDB:9bj1PDB:6nfyPDB:9czuPDB:6ng0PDB:8fh4PDB:9d00PDB:6cqdPDB:7r9nPDB:9bikPDB:9czwPDB:7l24PDB:7l25PDB:7r9tPDB:7kac

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	27894104

Sequence, Structure & Domains8

Sequences

Length

1,152

Mass

121,005

Sequence

MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI

Alternative Products

Event=Alternative splicing; Named isoforms=7; Comment=Additional isoforms seem to exist.; Name=1; IsoId=P27816-1; Sequence=Displayed; Name=2; IsoId=P27816-2; Sequence=VSP_003200; Name=3; IsoId=P27816-3; Sequence=VSP_032065, VSP_032068, VSP_032072, VSP_032073, VSP_032074, VSP_032075; Name=4; IsoId=P27816-4; Sequence=VSP_032066, VSP_032067, VSP_032069, VSP_032070, VSP_032076; Name=5; IsoId=P27816-5; Sequence=VSP_032071, VSP_032077, VSP_032079; Name=6; IsoId=P27816-6; Sequence=VSP_032078; Name=7; IsoId=P27816-7; Sequence=VSP_043240, VSP_043241

Alternative Sequence

1..151; Missing (in isoform 3); 1..82; Missing (in isoform 4); 83..221; LLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLE -> METTGDQGIEGMAYMDENRNITFTCPRTPSELINKSSPLEVLGSAACEKLPTPTPQVVKEGDSFPDT (in isoform 4); 98..99; GS -> EA (in isoform 7); 100..1152; Missing (in isoform 7); 152..437; LVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVA -> MSLSDKQTASLTAAYGQLSKGKPAECRMDSPKEISQAGFEWQRTEGKLNEIGLNVSMDGQPKDGLVKNASFLEQNKLCFFEGKLDKELSIEMQDKDCQEASGHLESRYVISETCHPLEGNSVHQKTSEFHLGLIEGPDKNKTIPVQGKVAGKNGLETKSQSDLDFPGAADIPTRYVKEQETSVWNPSFHPVAQGSLGSREATPGEMENSITPGCPVIGVVNDNSEQLKCESPLLVSLAHPAPIIEHSPTTIPPITMVFTQEHLNASCHIRDHDKELEK (in isoform 3); 225..271; EIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTE -> NGQEIAPAQISKSLMVDNYTKDGVPGQERPKGPSAVVPSTSTGG (in isoform 4); 275..666; AKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETS -> PITTAIETVNIHGDHSLKNKAELADSMKNEAGIDEGHVIGESESVHSGASKHSVEKVTELAKGHLLPGVPVEDQSLPGEARALEGYADRGNFPAHPVNEEKETKEGSVAVQIPDLLEDKAQKLSFCEDQNAQDRNSKGSDSLNKKVDLTLLSPKSENDKLKEISLACKITELESVSLPTPEIQSDFLHSKVEAPPSEVADTLVIMTASKGVRLPEPKDKILETPQKMTEKSESKTPGEGKKEDKSRMAEPMKGYMRPTKSRGLTPLLPKSTIQEQERHKQLKSA (in isoform 4); 322..666; WPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETS -> LPEPKDKILETPQKMTEKSESKTPGEGKKEDKSRMAEPMKGYMRPTKSRGLTPLLPKSTIQEQERHKQLKSA (in isoform 5); 441..631; ETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGT -> TEEAVLNQAPQQKKAVRRALSECSHLSVPPAVNLADKYPELPAREEPSSGLLPPPSSPMPSPTPGKLGAPAMKRSMTVGEEQTASYKLSPGKLPILSTKEIPPFICEEPVAKKREELAHFSNSSSNSGKKELGTAGLYLHSKLEQIPEGSSKEKGQEDFSETRIDSCSQVCQRGEKQPGQTALA (in isoform 3); 558..730; Missing (in isoform 2); 635..666; CSLPAEEDSVLEKLGERKPCNSQPSELSSETS -> EIEVTATQSTPSFLFEKPPRD (in isoform 3); 703..716; PLATTQPAKTSTSK -> VGARMVVIFYCHNF (in isoform 3); 717..1152; Missing (in isoform 3); 939..953; Missing (in isoform 4); 954..1022; Missing (in isoform 5); 1088..1152; TEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI -> IETYRLTFRANARARTDHGADIVSRPPHFPGGPNSGSRVLGPLSRAVH (in isoform 6); 1151..1152; SI -> N (in isoform 5)

Domain & Motif Annotations

Compositional Bias

53..67; Basic and acidic residues; 68..81; Polar residues; 371..384; Basic and acidic residues; 644..653; Basic and acidic residues; 654..666; Polar residues; 680..689; Polar residues; 704..725; Polar residues; 769..786; Basic and acidic residues; 788..819; Low complexity; 839..848; Basic and acidic residues; 861..878; Low complexity; 911..929; Polar residues; 1105..1119; Low complexity; 1142..1152; Polar residues

Repeat

248..261; 1; 262..275; 2; 276..289; 3; 290..303; 4; 304..317; 5; 318..331; 6; 332..345; 7; 346..351; 8; truncated; 352..377; 26 residues 1; 378..403; 26 residues 2; 408..421; 9; 422..433; 10; 434..447; 11; 448..461; 12; 462..475; 13; 476..489; 14; 490..503; 15; 504..517; 16; 532..545; 17; 923..953; Tau/MAP 1; 992..1022; Tau/MAP 2; 1023..1053; Tau/MAP 3; 1054..1085; Tau/MAP 4

Region

50..100; Disordered; 248..545; 17 X 14 AA tandem repeats; 362..401; Disordered; 641..991; Disordered; 1074..1152; Disordered

Clinical Relevance6

Disease Involvement

FDA approved drug targets

Drug Targets (5)

FDA approved drug targetsPatented-recorded targetLiterature-reported targetClinical trial targetSuccessful target

Antibody (2)

HPA038149 HPA038150

Interaction Protein (2)

ENSG00000014216ENSG00000044574

Interaction Count

Interaction Dataset (2)

intact_biogrid_opencellbiogrid_opencell

Supporting Publications21

PMID	Title	Abstract
26826536	[Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].	No abstract available
30915084	Extracellular Vesicles Mediate Mesenchymal Stromal Cell-Dependent Regulation of B Cell PI3K-AKT Signaling Pathway and Actin Cytoskeleton.	No abstract available
31382537	An Insight into the Proteome of Uveal Melanoma-Derived Ectosomes Reveals the Presence of Potentially Useful Biomarkers.	No abstract available
31805958	Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.	No abstract available
32560723	T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.	No abstract available
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
33309826	Proteomic analysis of extracellular vesicles and conditioned medium from human adipose-derived stem/stromal cells and dermal fibroblasts.	No abstract available
34064677	Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells.	No abstract available
36064647	Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.	No abstract available
36497184	Oxidative Stress and Extracellular Matrix Remodeling Are Signature Pathways of Extracellular Vesicles Released upon Morphine Exposure on Human Brain Microvascular Endothelial Cells.	No abstract available

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