Protein detail

MAP4

Microtubule-associated protein 4 (MAP-4)

Entry name MAP4	UniProt ID P27816	EVMP confidence score 0.75
Supporting publications (n) 21	Transmembrane count	Protein classification FDA approved drug targetsPlasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Microtubule-associated protein 4 (MAP-4)

Protein Class (3)

FDA approved drug targetsPlasma proteinsPredicted intracellular proteins

Protein Function (2)

FDA approved drug targets:Small molecule drugs
Predicted intracellular proteins

Ensembl

ENSG00000047849

Entrez Gene Symbol

MAP4

Gene Description

Microtubule associated protein 4

Chromosome

Position

47850690-48089272

Supporting publications (n)

EVMP confidence score

0.75

Fluorescence & Localization1

Function & Pathway5

Protein Function (2)

FDA approved drug targets:Small molecule drugs
Predicted intracellular proteins

Cellular Component (10)

Molecular Function (5)

Biological Process (3)

Mediation Categories

Clinical-translation mediation

Relations & Evidence58

Enzyme-Mediated Modification (33)

33 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
MAP4	CDK1	P06493	S	787	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:9398320 KEA:17570479 ProtMapper:10791892 HPRD:11683421 KEA:15216889 HPRD:20068231 SIGNOR:9398320 phosphoELM:9398320 HPRD:19691289 KEA:11683421 HPRD:19664995 SIGNOR:10791892 HPRD:9398320 HPRD:19415658 ProtMapper:9398320
MAP4	CDK1	P06493	S	696	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:9398320 HPRD:11683421 KEA:15216889 HPRD:20068231 SIGNOR:9398320 phosphoELM:9398320 KEA:11683421 HPRD:9398320 KEA:17570479 ProtMapper:9398320
MAP4	CDK1	P06493	T	917	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
MAP4	CDK1	P06493	T	901	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
MAP4	CDK1	P06493	T	892	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
MAP4	CDK1	P06493	S	431	phosphorylation	KEA	KEA:9398320 KEA:11683421 KEA:15216889
MAP4	CDK1	P06493	S	522	phosphorylation	KEA	KEA:9398320 KEA:11683421 KEA:15216889
MAP4	MARK1	Q9P0L2	S	1,073	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	HPRD:18707149 HPRD:17693683 HPRD:19651622 HPRD:20068231 HPRD:8631898 KEA:17081983 HPRD:18212344 HPRD:18669648 HPRD:19415658 ProtMapper:8631898 SIGNOR:8631898 HPRD:20166139 KEA:8631898 HPRD:18452278
MAP4	MARK1	Q9P0L2	S	928	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	HPRD:18767875 HPRD:20058876 HPRD:20068231 HPRD:8631898 ProtMapper:8631898 SIGNOR:8631898 KEA:8631898 HPRD:20166139
MAP4	MARK1	Q9P0L2	S	941	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	HPRD:20068231 HPRD:8631898 HPRD:18669648 ProtMapper:8631898 SIGNOR:8631898 KEA:8631898 HPRD:20166139

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Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MARK1	Q9P0L2	MAP4	P27816	Yes	No	Yes	SPIKEphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phosSPIKE_LC	HPRD-phos:19415658 HPRD-phos:18669648 ProtMapper:19415658 ProtMapper:18707149 SPIKE:8631898 HPRD-phos:18767875 SPIKE_LC:8631898 SPIKE:10542369 HPRD:8631898 KEA:17081983 HPRD-phos:20068231 ProtMapper:8631898 ProtMapper:17693683 ProtMapper:18212344 HPRD-phos:18452278 ProtMapper:18767875 HPRD-phos:20058876 ProtMapper:20058876 ProtMapper:19651622 HPRD-phos:18212344 SIGNOR:8631898 HPRD-phos:18707149 HPRD-phos:8631898 ProtMapper:20166139 ProtMapper:20068231 ProtMapper:18669648 ProtMapper:18452278 HPRD-phos:19651622 KEA:8631898 HPRD-phos:17693683 SPIKE_LC:10542369 HPRD-phos:20166139
CHK1	O14757	MAP4	P27816	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperPhosphoSite_ProtMapper	SIGNOR:36991467
CDK1	P06493	MAP4	P27816	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAHPRDWangPhosphoSite_ProtMapperNetworKIN_KEAphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteHPRD-phos	ProtMapper:10791892 HPRD-phos:19415658 HPRD:11683421 phosphoELM:9398320 KEA:11683421 ProtMapper:19415658 HPRD-phos:19691289 HPRD-phos:9398320 KEA:15216889 SIGNOR:9398320 HPRD-phos:20068231 HPRD:9398320 ProtMapper:11683421 PhosphoSite:10791892 KEA:9398320 ProtMapper:9398320 ProtMapper:19691289 ProtMapper:20068231 HPRD-phos:11683421 SIGNOR:10791892 KEA:17570479 ProtMapper:19664995 HPRD-phos:19664995
TTK	P33981	MAP4	P27816	Yes	No	Yes	PhosphoSite_norefSIGNORProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:31253867 PhosphoSite:31253867
MK14	Q16539	MAP4	P27816	Yes	No	No	PhosphoSite_ProtMapperPhosphoSite_norefProtMapperKEAPhosphoSiteNetworKIN_KEA	KEA:17570479 PhosphoSite:9398320 PhosphoSite:25746230

Protein Complex Composition (14)

14 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HT_DM_Cluster155	CASP9 MAP4K4 MINK1 SARM1 TNIK VMA12 ZNF354B	O95819 P55211 Q6SZW1 Q8N4C8 Q8N511 Q96LW1 Q9UKE5	1:1:1:1:1:1:1	Compleat	Compleat:HC1461	22036573
	DDX39B DHX38 MAGOHB MAP4 RBM8A RPS20 SLU7 TFCP2L1	O95391 P27816 P60866 Q13838 Q92620 Q96A72 Q9NZI6 Q9Y5S9	0:0:0:0:0:0:0:0	Havugimana2012	Havugimana2012:C_554
	MAP4K4	O95819	2	PDB	PDB:4u40PDB:4rvtPDB:8v5iPDB:5j95PDB:5di1PDB:4u3zPDB:4zp5PDB:4oboPDB:4u45PDB:4u43PDB:5w5qPDB:4obpPDB:4zk5PDB:4u41PDB:4obqPDB:4u44PDB:4u42PDB:4u3y
	KIF5A MAP4K4 MDM2	O95819 Q00987 Q12840	1:1:1	CompleatCFinder	Compleat:HC8257
	IPO9 MAP4K4 RAP2C	O95819 Q96P70 Q9Y3L5	0:0:0	hu.MAP2
	MAP4K4 RAP2C	O95819 Q9Y3L5	0:0	hu.MAP2
	ARHGAP27 ARHGAP31 CDC42 MAP4K3 NEB PAK2 PLEKHG2 RAC1 SH3PXD2A SH3RF1	P20929 P60953 P63000 Q13177 Q2M1Z3 Q5TCZ1 Q6ZUM4 Q7Z6J0 Q8IVH8 Q9H7P9	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8529
	ARHGAP31 CDC42 MAP3K9 MAP4K3 NEB PAK2 PLEKHG2 SH3PXD2A SH3RF1	P20929 P60953 P80192 Q13177 Q2M1Z3 Q5TCZ1 Q7Z6J0 Q8IVH8 Q9H7P9	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8147
	EIF4B MAP4	P23588 P27816	0:0	hu.MAPhu.MAP2
	EIF4B MAP4 SH3GLB1	P23588 P27816 Q9Y371	0:0:0	hu.MAP2

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	27894104

Sequence, Structure & Domains8

Sequences

Length

1,152

Mass

121,005

Sequence

MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI

Alternative Products

Event=Alternative splicing; Named isoforms=7; Comment=Additional isoforms seem to exist.; Name=1; IsoId=P27816-1; Sequence=Displayed; Name=2; IsoId=P27816-2; Sequence=VSP_003200; Name=3; IsoId=P27816-3; Sequence=VSP_032065, VSP_032068, VSP_032072, VSP_032073, VSP_032074, VSP_032075; Name=4; IsoId=P27816-4; Sequence=VSP_032066, VSP_032067, VSP_032069, VSP_032070, VSP_032076; Name=5; IsoId=P27816-5; Sequence=VSP_032071, VSP_032077, VSP_032079; Name=6; IsoId=P27816-6; Sequence=VSP_032078; Name=7; IsoId=P27816-7; Sequence=VSP_043240, VSP_043241

Alternative Sequence

1..151; Missing (in isoform 3); 1..82; Missing (in isoform 4); 83..221; LLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLE -> METTGDQGIEGMAYMDENRNITFTCPRTPSELINKSSPLEVLGSAACEKLPTPTPQVVKEGDSFPDT (in isoform 4); 98..99; GS -> EA (in isoform 7); 100..1152; Missing (in isoform 7); 152..437; LVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVA -> MSLSDKQTASLTAAYGQLSKGKPAECRMDSPKEISQAGFEWQRTEGKLNEIGLNVSMDGQPKDGLVKNASFLEQNKLCFFEGKLDKELSIEMQDKDCQEASGHLESRYVISETCHPLEGNSVHQKTSEFHLGLIEGPDKNKTIPVQGKVAGKNGLETKSQSDLDFPGAADIPTRYVKEQETSVWNPSFHPVAQGSLGSREATPGEMENSITPGCPVIGVVNDNSEQLKCESPLLVSLAHPAPIIEHSPTTIPPITMVFTQEHLNASCHIRDHDKELEK (in isoform 3); 225..271; EIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTE -> NGQEIAPAQISKSLMVDNYTKDGVPGQERPKGPSAVVPSTSTGG (in isoform 4); 275..666; AKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETS -> PITTAIETVNIHGDHSLKNKAELADSMKNEAGIDEGHVIGESESVHSGASKHSVEKVTELAKGHLLPGVPVEDQSLPGEARALEGYADRGNFPAHPVNEEKETKEGSVAVQIPDLLEDKAQKLSFCEDQNAQDRNSKGSDSLNKKVDLTLLSPKSENDKLKEISLACKITELESVSLPTPEIQSDFLHSKVEAPPSEVADTLVIMTASKGVRLPEPKDKILETPQKMTEKSESKTPGEGKKEDKSRMAEPMKGYMRPTKSRGLTPLLPKSTIQEQERHKQLKSA (in isoform 4); 322..666; WPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETS -> LPEPKDKILETPQKMTEKSESKTPGEGKKEDKSRMAEPMKGYMRPTKSRGLTPLLPKSTIQEQERHKQLKSA (in isoform 5); 441..631; ETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGT -> TEEAVLNQAPQQKKAVRRALSECSHLSVPPAVNLADKYPELPAREEPSSGLLPPPSSPMPSPTPGKLGAPAMKRSMTVGEEQTASYKLSPGKLPILSTKEIPPFICEEPVAKKREELAHFSNSSSNSGKKELGTAGLYLHSKLEQIPEGSSKEKGQEDFSETRIDSCSQVCQRGEKQPGQTALA (in isoform 3); 558..730; Missing (in isoform 2); 635..666; CSLPAEEDSVLEKLGERKPCNSQPSELSSETS -> EIEVTATQSTPSFLFEKPPRD (in isoform 3); 703..716; PLATTQPAKTSTSK -> VGARMVVIFYCHNF (in isoform 3); 717..1152; Missing (in isoform 3); 939..953; Missing (in isoform 4); 954..1022; Missing (in isoform 5); 1088..1152; TEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI -> IETYRLTFRANARARTDHGADIVSRPPHFPGGPNSGSRVLGPLSRAVH (in isoform 6); 1151..1152; SI -> N (in isoform 5)

Domain & Motif Annotations

Compositional Bias

53..67; Basic and acidic residues; 68..81; Polar residues; 371..384; Basic and acidic residues; 644..653; Basic and acidic residues; 654..666; Polar residues; 680..689; Polar residues; 704..725; Polar residues; 769..786; Basic and acidic residues; 788..819; Low complexity; 839..848; Basic and acidic residues; 861..878; Low complexity; 911..929; Polar residues; 1105..1119; Low complexity; 1142..1152; Polar residues

Repeat

248..261; 1; 262..275; 2; 276..289; 3; 290..303; 4; 304..317; 5; 318..331; 6; 332..345; 7; 346..351; 8; truncated; 352..377; 26 residues 1; 378..403; 26 residues 2; 408..421; 9; 422..433; 10; 434..447; 11; 448..461; 12; 462..475; 13; 476..489; 14; 490..503; 15; 504..517; 16; 532..545; 17; 923..953; Tau/MAP 1; 992..1022; Tau/MAP 2; 1023..1053; Tau/MAP 3; 1054..1085; Tau/MAP 4

Region

50..100; Disordered; 248..545; 17 X 14 AA tandem repeats; 362..401; Disordered; 641..991; Disordered; 1074..1152; Disordered

Clinical Relevance6

Disease Involvement

FDA approved drug targets

Drug Targets (5)

FDA approved drug targetsPatented-recorded targetLiterature-reported targetClinical trial targetSuccessful target

Antibody (2)

HPA038149 HPA038150

Interaction Protein (2)

ENSG00000014216ENSG00000044574

Interaction Count

Interaction Dataset (2)

intact_biogrid_opencellbiogrid_opencell

Supporting Publications21

PMID	Title	Abstract
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
38590132	Proteomic Identification of Small Extracellular Vesicle Proteins LAMB1 and Histone H4 for Prostate Cancer Diagnosis and Risk Stratification.	No abstract available
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available
39207047	The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available
40465195	Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.	No abstract available
40840701	Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma.	No abstract available
40985879	TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins.	No abstract available

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