Protein detail

EPHA3

Ephrin type-A receptor 3 (EC 2.7.10.1) (EPH-like kinase 4) (EK4) (hEK4) (HEK) (Human embryo kinase) (Tyrosine-protein kinase TYRO4) (Tyrosine-protein kinase receptor ETK1) (Eph-like tyrosine kinase 1)

Entry name EPHA3	UniProt ID P29320	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 1	Protein classification Cancer-related genesDisease related genesEnzymesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Protein Class (6)

Cancer-related genesDisease related genesEnzymesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (7)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Kinases:Tyr protein kinases
Disease related genes

Transmembrane

542..565; Helical

Transmembrane Count

Ensembl

ENSG00000044524

Entrez Gene Symbol

EPHA3

Gene Synonym (5)

ETKETK1HEKHEK4TYRO4

Gene Description

EPH receptor A3

Chromosome

Position

89107621-89482134

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization4

Cell SpecificSyncytiotrophoblastsSecretome LocationSecreted - unknown locationSecretome FunctionReceptor

Function & Pathway8

Protein Function (7)

Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Kinases:Tyr protein kinases
Disease related genes

Cellular Component (8)

Molecular Function (5)

Biological Process (3)

KEGG

hsa04360 Axon guidance

Reactome (4)

Canonical Pathways (3)

M5880 Naba ecm affiliated
M5885 Naba matrisome associated
M5889 Naba matrisome

Mediation Categories (4)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence81

Enzyme-Mediated Modification (3)

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EPHA3	PTPN1	P18031	Y	779	dephosphorylation	SIGNORDEPOD	DEPOD:21135139 SIGNOR:21135139
EPHA3	PTPN1	P18031	Y	779	phosphorylation	SIGNOR_ProtMapperProtMapper	ProtMapper:21135139
EPHA3	LCK	P06239	Y	602	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (66)

66 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
plasma_membrane	plasma_membrane	OmniPath	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	Membranome	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	CSPA	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	HPMR	No	No	Yes	Yes	No
plasma_membrane_transmembrane	plasma_membrane_transmembrane	OmniPath	No	No	Yes	Yes	No
secreted	secreted	UniProt_keyword	No	No	Yes	Yes	No
secreted	secreted	UniProt_location	No	No	Yes	Yes	No
secreted	secreted	OmniPath	No	No	Yes	Yes	No
cell_surface	cell_surface	Surfaceome	No	No	Yes	Yes	No
cell_surface	cell_surface	connectomeDB2020	No	No	Yes	Yes	No

Page 5 of 7Previous Next

Regulatory Interaction Network (11)

11 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
EFNA1	P20827	EPHA3	P29320	Yes	Yes	No	iTALKICELLNETSIGNORCellPhoneDB_CellinkerSignaLink3HPMR_talklrCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDDLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrCellinkerSTRING_talklrCellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	connectomeDB2020:8070404 Cellinker:9330863 SignaLink3:18988627 Cellinker:8070404 ICELLNET:24003208 SignaLink3:8070404 SIGNOR:9330863 Cellinker:15114347 LRdb:11114742 SignaLink3:9195962 CellChatDB:15114347 ICELLNET:15114347 SignaLink3:9576626 SignaLink3:23331499 HPRD:8070404 SIGNOR:9576626 connectomeDB2020:9330863 SignaLink3:21071413
EFNA5	P52803	EPHA3	P29320	Yes	Yes	No	iTALKICELLNETSIGNORHINTCellPhoneDB_CellinkerSignaLink3HPMR_talklrHPMRCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDDLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBioGRIDCellinkerSTRING_talklrCellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	HINT:9195962 Cellinker:9330863 HPRD:9751130 SignaLink3:9245480 HINT:19823572 Cellinker:15114347 connectomeDB2020:9751130 SignaLink3:9195962 SignaLink3:9751130 Cellinker:9751130 HINT:9245480 Cellinker:9195962 SignaLink3:18988627 connectomeDB2020:9195962 HPRD:9195962 LRdb:11114742 Cellinker:9245480 BioGRID:22242939 CellChatDB:15114347 HINT:25993310 HPRD:9245480 SignaLink3:23331499 HINT:22242939 connectomeDB2020:9245480 connectomeDB2020:9330863 HINT:33961781 SignaLink3:21071413 HPMR:9330863 ICELLNET:24003208 SIGNOR:9330863 ICELLNET:15114347 SignaLink3:9576626 BioGRID:9245480
EPHA3	P29320	CRK	P46108	Yes	Yes	No	WangPhosphoPointSIGNORHPRDSignaLink3SPIKE_LC	SignaLink3:18988627 SignaLink3:11870224 SPIKE_LC:16713569 SignaLink3:23331499 HPRD:11870224 SignaLink3:21071413 SIGNOR:11870224
EFNA3	P52797	EPHA3	P29320	Yes	Yes	No	iTALKICELLNETSIGNORCellPhoneDB_CellinkerSignaLink3HPMR_talklrCellChatDBDLRP_CellinkertalklrDLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBCellinkerCellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:9330863 ICELLNET:24003208 SIGNOR:9330863 Cellinker:15114347 LRdb:11114742 SignaLink3:9195962 ICELLNET:15114347 CellChatDB:15114347 SignaLink3:23331499 SignaLink3:9267020 connectomeDB2020:9330863
EFNA4	P52798	EPHA3	P29320	Yes	Yes	No	CellChatDBCellTalkDBDLRP_CellinkeriTALKCellPhoneDBtalklrICELLNETRamilowski2015connectomeDB2020CellPhoneDB_CellinkerSignaLink3DLRP_talklrCellinkerLRdbCellCall	Cellinker:28589441 connectomeDB2020:8755474 ICELLNET:24003208 talklr:8755474 Cellinker:15114347 LRdb:11114742 Ramilowski2015:8755474 SignaLink3:9195962 ICELLNET:15114347 CellChatDB:15114347 SignaLink3:9576626 SignaLink3:23331499
EFNB2	P52799	EPHA3	P29320	Yes	Yes	No	Fantom5_LRdbCellTalkDBHPRD_LRdbiTALKtalklrconnectomeDB2020HPRDHINTHPRD_talklrBioGRIDSignaLink3CellinkerLRdbSTRING_talklrRamilowski2015	HINT:9195962 BioGRID:8559144 HINT:8559144 SignaLink3:8559144 connectomeDB2020:8559144 SignaLink3:9195962 LRdb:8559144 SignaLink3:23331499 CellTalkDB:8559144 HPRD:8559144 Cellinker:32589946
EPHA3	P29320	SRC	P12931	Yes	Yes	No	SIGNORSignaLink3	SignaLink3:23331499 SIGNOR:9632142 SignaLink3:9632142
EPHA3	P29320	FYN	P06241	Yes	Yes	No	SignaLink3	SignaLink3:23331499 SignaLink3:9632142
PTN1	P18031	EPHA3	P29320	Yes	No	Yes	SIGNOR_ProtMapperSIGNORProtMapperDEPOD	DEPOD:21135139 ProtMapper:21135139 SIGNOR:21135139
EPHA3	P29320	ANDR	P10275	Yes	Yes	No	REACH_ProtMapperSIGNORSparser_ProtMapperProtMapper	ProtMapper:20570899 SIGNOR:20570899 ProtMapper:23752182

Page 1 of 2Next

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	29441425

Sequence, Structure & Domains13

Sequences

Length

983

Mass

110,131

Sequence

MDCQLSILLLLSCSVLDSFGELIPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKALDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPPSSPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNIKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVLTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRARGTNVTISSLKPDTIYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGESSQVVMIAISAAVAIILLTVVIYVLIGRFCGYKSKHGADEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNVDITTFRTTGDWLNGVWTAHCKEIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIISSIKALETQSKNGPVPV

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P29320-1; Sequence=Displayed; Name=2; IsoId=P29320-2; Sequence=VSP_002995, VSP_002996

Alternative Sequence

532..539; SFSISGES -> CMYYFNAV (in isoform 2); 540..983; Missing (in isoform 2)

3D Structural Models

Turn

38..41; 610..612; 713..716; 819..821; 824..827

Helix

106..108; 137..139; 154..159; 618..620; 661..674; 707..712; 720..739; 749..751; 765..768; 788..790; 793..798; 803..818; 830..838; 851..860; 865..867; 871..883; 885..889

Beta Strand

29..34; 45..48; 51..58; 64..71; 75..77; 80..83; 96..104; 117..128; 130..132; 141..147; 165..170; 176..200; 621..629; 631..641; 647..654; 685..689; 691..694; 696..700; 752..754; 760..762; 890..892; 898..903

3D Structure

X-ray crystallography (28)

Domain & Motif Annotations

Motif

981..983; PDZ-binding

Domain (FT)

29..207; Eph LBD; 325..435; Fibronectin type-III 1; 436..531; Fibronectin type-III 2; 621..882; Protein kinase; 911..975; SAM

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
Ephrin receptor subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Clinical Relevance8

Disease Involvement

Cancer-related genes

Related Diseases (4)

Acute myeloid leukaemia Brain cancer Malignant haematopoietic neoplasm Myelodysplastic syndrome

Biomarker

Phase 2; Phase 1; Phase 1/2

Drugs (9)

IRAK-1/4 INHIBITOR BELIZATINIB TAKINIB VANDETANIB KB-004 IFABOTUZUMAB HESPERADIN COMPOUND 1 [WO2012007375]COMPOUND 7 [WO2012007375]

Antibody (2)

CAB010462 HPA069390

Interaction Protein (2)

ENSG00000135333ENSG00000142627

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
29635386	Amniotic Fluid Exosome Proteomic Profile Exhibits Unique Pathways of Term and Preterm Labor.	Exosomes were isolated by differential centrifugation and quantified using nanocrystals (Qdot) coupled to CD63 and placental alkaline phosphatase (PLAP) by fluorescence nanoparticle tracking analysis.