Protein detail

NOS1

Nitric oxide synthase 1 (EC 1.14.13.39) (Constitutive NOS) (NC-NOS) (NOS type I) (Neuronal NOS) (N-NOS) (nNOS) (Nitric oxide synthase, brain) (bNOS) (Peptidyl-cysteine S-nitrosylase NOS1)

Entry name NOS1	UniProt ID P29475	EVMP score 0.50
Frequency 1	Transmembrane count	Protein classification Cancer-related genesEnzymesFDA approved drug targetsMetabolic proteinsPlasma proteinsPredicted intracellular proteinsTransporters

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Nitric oxide synthase 1 (EC 1.14.13.39) (Constitutive NOS) (NC-NOS) (NOS type I) (Neuronal NOS) (N-NOS) (nNOS) (Nitric oxide synthase, brain) (bNOS) (Peptidyl-cysteine S-nitrosylase NOS1)

Protein Class

Cancer-related genesEnzymesFDA approved drug targetsMetabolic proteinsPlasma proteinsPredicted intracellular proteinsTransporters

Protein Function

ENZYME proteins:Oxidoreductases
Predicted intracellular proteins
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Transporters:Accessory Factors Involved in Transport
FDA approved drug targets:Small molecule drugs

Ensembl

ENSG00000089250

Entrez Gene Symbol

NOS1

Gene Synonym

nNOSNOS

Gene Description

Nitric oxide synthase 1

Chromosome

Position

117208142-117452170

Frequency

EVMP Score

0.50

Fluorescence & Localization

Tissue Specificsalivary glandCell SpecificFoveolar cellsSingle-Nuclei Brain SpecificastrocyteBlood Cell Specificplasmacytoid DC

Function & Pathway

Protein Function

ENZYME proteins:Oxidoreductases
Predicted intracellular proteins
Enzymes
Cancer-related genes:Candidate cancer biomarkers
Transporters:Accessory Factors Involved in Transport
FDA approved drug targets:Small molecule drugs

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Mediation Categories

Clinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

33 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
NOS1	CAMK2A	Q9UQM7	S	852	phosphorylation	SIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapper	KEA:1375933 ProtMapper:10400690 phosphoELM:12630910 KEA:10874031 KEA:10400690 KEA:12630910 phosphoELM:10400690 SIGNOR:10400690 phosphoELM:10874031
NOS1	CAMK2A	Q9UQM7	S	886	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
NOS1	CAMK4	Q16566	S	852	phosphorylation	SIGNOR_ProtMapperKEASIGNORProtMapper	KEA:1375933 ProtMapper:10400690 KEA:10874031 KEA:10400690 KEA:12630910 SIGNOR:10400690
NOS1	CAMK4	Q16566	S	886	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
NOS1	CAMK1	Q14012	S	852	phosphorylation	SIGNORProtMapperKEASIGNOR_ProtMapperREACH_ProtMapper	KEA:1375933 ProtMapper:10400690 KEA:10874031 KEA:10400690 ProtMapper:27885874 KEA:12630910 SIGNOR:10400690
NOS1	CAMK1	Q14012	S	746	phosphorylation	MIMPHPRDHPRD_MIMPKEA	HPRD:15251453 KEA:15251453
NOS1	CAMK1	Q14012	S	886	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
NOS1	PRKACA	P17612	S	886	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
NOS1	PRKACA	P17612	S	852	phosphorylation	KEA	KEA:1375933 KEA:10874031 KEA:10400690 KEA:12630910
NOS1	PRKCA	P17252	S	886	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP

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Ligand-Receptor Signaling

8 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

9 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
NOS1	P29475	HDAC2	Q92769	Yes	Yes	Yes	SPIKESPIKE_LCSIGNOR	SPIKE:19737086 SIGNOR:19047631 SPIKE_LC:19737086
KPCA	P17252	NOS1	P29475	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEACA1HPRD_KEAWang	KEA:1375933 ProtMapper:1375933 SIGNOR:1375933 ProtMapper:12630910 HPRD:12630910 HPRD:1375933 ProtMapper:10400690 KEA:10874031 ProtMapper:10874031 KEA:10400690 CA1:7694293 HPRD:10400690 KEA:12630910 HPRD:10874031
KCC2A	Q9UQM7	NOS1	P29475	Yes	Yes	Yes	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEACA1phosphoELM_KEAHPRD_KEAphosphoELMSIGNOR_ProtMapper	KEA:1375933 ProtMapper:1375933 phosphoELM:10874031 ProtMapper:12630910 HPRD:12630910 CA1:10400690 HPRD:1375933 ProtMapper:10400690 phosphoELM:12630910 KEA:10874031 CA1:10874031 KEA:10400690 ProtMapper:10874031 HPRD:10400690 KEA:12630910 phosphoELM:10400690 SIGNOR:10400690 HPRD:10874031
KCC4	Q16566	NOS1	P29475	Yes	No	Yes	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEACA1phosphoELM_KEAHPRD_KEASIGNOR_ProtMapper	KEA:1375933 ProtMapper:1375933 ProtMapper:12630910 HPRD:12630910 CA1:10400690 HPRD:1375933 ProtMapper:10400690 KEA:10874031 ProtMapper:10874031 KEA:10400690 HPRD:10400690 KEA:12630910 SIGNOR:10400690 HPRD:10874031
KAPCA	P17612	NOS1	P29475	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEACA1HPRD_KEAWang	CA1:7532810 KEA:1375933 ProtMapper:1375933 SIGNOR:1375933 ProtMapper:12630910 HPRD:12630910 HPRD:1375933 ProtMapper:10400690 KEA:10874031 ProtMapper:10874031 KEA:10400690 HPRD:10400690 KEA:12630910 HPRD:10874031
DLG4	P78352	NOS1	P29475	Yes	Yes	No	SIGNORHPRDCui2007HINTCA1WangLit-BM-17	Lit-BM-17:8625413 CA1:8922396 HINT:9459447 HPRD:8625413 SIGNOR:11052931 HPRD:9459447 HPRD:10488080 HINT:10488080 HINT:8625413 HINT:26941280 CA1:11031113 Lit-BM-17:9459447
SNTA1	Q13424	NOS1	P29475	Yes	Yes	No	HPRDHINTSIGNOR	HPRD:10221915 HINT:9412493 HPRD:11747091 SIGNOR:12456711 HINT:33961781
KPCD1	Q15139	NOS1	P29475	Yes	Yes	No	Lit-BM-17SIGNORSparser_ProtMapperProtMapper	ProtMapper:24740233 Lit-BM-17:24740233 SIGNOR:24740233
KCC1A	Q14012	NOS1	P29475	Yes	No	Yes	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAphosphoELM_KEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperHPRD-phos	KEA:1375933 ProtMapper:1375933 ProtMapper:12630910 HPRD:12630910 ProtMapper:15251453 HPRD:1375933 ProtMapper:10400690 KEA:10874031 ProtMapper:10874031 KEA:10400690 ProtMapper:27885874 HPRD:15251453 KEA:15251453 HPRD-phos:15251453 HPRD:10400690 KEA:12630910 SIGNOR:10400690 HPRD:10874031

Protein Complex Composition

9 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
C1orf226 NKAP NKAPL NOS1AP	A1L170 O75052 Q5M9Q1 Q8N5F7	0:0:0:0	hu.MAP2
CNOT1 NANOS1	A5YKK6 Q8WY41	2:2	PDB	PDB:4cqo
EPM2A GYG1 GYG2 GYS1 GYS2 NANOS1 PPP1R3C STBD1	B3EWF7 O15488 O95210 P13807 P46976 P54840 Q8WY41 Q9UQK1	0:0:0:0:0:0:0:0	hu.MAP2
EPM2A GYG1 GYG2 GYS1 NANOS1 PPP1R3C STBD1	B3EWF7 O15488 O95210 P13807 P46976 Q8WY41 Q9UQK1	0:0:0:0:0:0:0	hu.MAP2
EPM2A GYG1 GYG2 GYS1 GYS2 NANOS1 PPP1R3C STBD1	O15488 O95210 O95278 P13807 P46976 P54840 Q8WY41 Q9UQK1	0:0:0:0:0:0:0:0	hu.MAP2
EPM2A GYG1 GYG2 GYS1 NANOS1 PPP1R3C STBD1	O15488 O95210 O95278 P13807 P46976 Q8WY41 Q9UQK1	0:0:0:0:0:0:0	hu.MAP2
B9D1 NOS1 NOS1AP PRKACA USP8 YWHAZ	O75052 P17612 P29475 P40818 P63104 Q9UPM9	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7828
NOS1	P29475	4	PDB	PDB:5fvxPDB:8fghPDB:7ts5PDB:8ufpPDB:6ngcPDB:7ts3PDB:5vv5PDB:7ts7PDB:6ngdPDB:7us8PDB:6pobPDB:8fglPDB:7ts2PDB:8fgiPDB:8bi9PDB:7uamPDB:6ngaPDB:9cw0PDB:6pnhPDB:5uo1PDB:9cw3PDB:6pnaPDB:6auzPDB:5uo2PDB:6av0PDB:6av5PDB:6pndPDB:6nhcPDB:6po8PDB:9cw1PDB:8fgkPDB:6pnfPDB:9cvuPDB:6po5PDB:5vv0PDB:6po7PDB:5uo4PDB:7us7PDB:4v3uPDB:6ng1PDB:7ts1PDB:6av4PDB:6pocPDB:4uh6PDB:6ngePDB:6cidPDB:8fgjPDB:6ngiPDB:9cvzPDB:5vv1PDB:6ng6PDB:9cvxPDB:6av3PDB:6av1PDB:5fvvPDB:5vv4PDB:9cvvPDB:5uo7PDB:5uo5PDB:5fvwPDB:5vuvPDB:5fvuPDB:5uo6PDB:4uh5PDB:6potPDB:5adiPDB:6pnbPDB:6nghPDB:9cvwPDB:6nhbPDB:6pncPDB:6ng8PDB:6po9PDB:5adgPDB:6pngPDB:6auyPDB:6ng4PDB:9c1yPDB:5vuzPDB:5vv2PDB:8fgmPDB:6ng2PDB:6cicPDB:6poaPDB:4uchPDB:8ufqPDB:8fggPDB:7ts4PDB:5vv3PDB:6av2PDB:5uo3PDB:8bi8PDB:8fgfPDB:7ts8PDB:6ngfPDB:6ng7PDB:5adfPDB:9cvyPDB:6ngbPDB:7ts6PDB:5vuyPDB:5vuwPDB:6pnePDB:4d1nPDB:9cvsPDB:6ng5PDB:5vux
CDC5L MTREX NOS1 PPP2R1A PPP2R2D RBM7 ZCCHC8	P29475 P30153 P42285 Q66LE6 Q6NZY4 Q99459 Q9Y580	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5655

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Western blottingELISA	4	38731868 26775013 26801919 37922300

Sequence, Structure & Domains

Sequences

Length

1,434

Mass

160,970

Sequence

MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Alternative Products

Event=Alternative splicing; Named isoforms=5; Comment=Isoform 3 is produced by different alternative splicing events implicating either the untranslated exons TEX1 (TN-NOS) or TEX1B (TN-NOSB) leading to a N-terminally truncated protein which possesses enzymatic activity comparable to that of isoform 1. The C-terminally truncated isoform 4 is produced by insertion of the TEX2 exon between exons 3 and 4 of isoform 1, leading to a frameshift and a premature stop codon.; Name=1; Synonyms=N-NOS-1; IsoId=P29475-1; Sequence=Displayed; Name=2; Synonyms=N-NOS-2; IsoId=P29475-2; Sequence=VSP_003574; Name=3; Synonyms=TN-NOS, TN-NOSB; IsoId=P29475-3; Sequence=VSP_003571; Name=4; Synonyms=TEX2-insertion; IsoId=P29475-4; Sequence=VSP_003572, VSP_003573; Name=5; Synonyms=nNOSmu; IsoId=P29475-5; Sequence=VSP_044916

Alternative Sequence

1..336; Missing (in isoform 3); 285..407; PPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELI -> MRKLRITEGFGVQRGSHNHPPPQENSPPQRMAAPPSVHASSRSRTGRLRWFSLTPSTLRAHWKRDALSTSAWAPSCILLSMQGGLKTSAQKDSSSLSPKSLLINTIHQLKDLAPKPTWKGWKR (in isoform 4); 408..1434; Missing (in isoform 4); 509..613; Missing (in isoform 2); 844; K -> KYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHR (in isoform 5)

3D Structural Models

Turn

311..313; 606..609

Helix

321..324; 344..346; 356..373; 380..396; 403..415; 423..425; 440..455; 456..458; 501..503; 504..512; 543..545; 557..562; 595..599; 601..604; 612..618; 626..628; 630..648; 656..674; 681..684; 690..692; 694..697; 715..718

Beta Strand

105..111; 117..123; 306..310; 316..319; 333..336; 351..353; 377..379; 430..433; 463..466; 471..475; 481..485; 489..491; 493..495; 497..499; 520..522; 527..530; 537..539; 548..550; 565..568; 576..579; 582..585; 687..689; 702..704; 706..711

3D Structure

X-ray crystallography (106)

Domain & Motif Annotations

Compositional Bias

285..299; Polar residues

Domain (CC)

The PDZ domain participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes. Mediates interaction with VAC14..

Domain (FT)

17..99; PDZ; 760..940; Flavodoxin-like; 995..1242; FAD-binding FR-type

Region

1..205; Interaction with NOSIP; 112..192; Disordered; 163..245; Interaction with DYNLL1/PIN; 276..302; Disordered; 730..750; Calmodulin-binding

Protein Families

NOS family

Sequence Similarities

Belongs to the NOS family.

Clinical Relevance

Disease Involvement

Cancer-related genesFDA approved drug targets

Related Diseases

Headache Migraine

Biomarker

Phase 2

Drug Targets

FDA approved drug targets

Drugs

RECOMBINANT 70-KD HEAT-SHOCK PROTEIN NULL PRASTERONE DIURETIC CIMETIDINE PENICILLIN NIMESULIDE ANTI-ULCER AGENT THERAPEUTIC ANDROSTANOLONE THIOURACIL LIOTHYRONINE FLUOROURACIL SIROLIMUS ACETAZOLAMIDE TESTOSTERONE BACLOFEN IBUPROFEN, SODIUM SALT ADJUVANT ANTIBIOTIC THEOPHYLLINE PROPOFOL STAUROSPORINE PRAVASTATIN SODIUM ANAKINRA TILARGININE ACETATE CYCLOPHOSPHAMIDE ANHYDROUS BENZODIAZEPINE SODIUM CHLORIDE TIRAPAZAMINE IDOXIFENE TILARGININE RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST ATORVASTATIN CALCIUM TRIHYDRATE ENZYME INHIBITOR METHYLTESTOSTERONE EPIRUBICIN COMPOUND 14J [PMID: 27050842]RECOMBINANT NEUROTROPHIC FACTOR NXN-188 CAFFEINE ANALGESIC AGENT GABAPENTIN ENALAPRIL MALEATE L-NNA THERAPEUTIC CORTICOSTEROID GREEN TEA LEAF EXTRACT CHEMOPREVENTIVE AGENT ANTIDEPRESSANT AGENT PRAZOSIN ZZL-7 3-BROMO-7NI DEXTRAN SULFATE SODIUM TRETINOIN BUTHIONINE SULFOXIMINE ANTIESTROGEN CARVEDILOL BACILLUS CALMETTE-GUERIN ANTIGEN GINKGO BILOBA EXTRACT MECHLORETHAMINE FULVESTRANT ROSIGLITAZONE 7NI POLYESTRADIOL PHOSPHATE NONSTEROIDAL ANTIINFLAMMATORY DRUG AZITHROMYCIN CLARITHROMYCIN SOY PROTEIN ISOLATE RESINIFERATOXIN IH636 GRAPE SEED PROANTHOCYANIDIN EXTRACT DITIOCARB PHENYLEPHRINE GW274150

Antibody

CAB002167 HPA058312 HPA069509