Protein detail

GRK4

G protein-coupled receptor kinase 4 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK4) (ITI1)

Entry name GRK4	UniProt ID P32298	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

G protein-coupled receptor kinase 4 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK4) (ITI1)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000125388

Entrez Gene Symbol

GRK4

Gene Synonym (2)

GPRK2LGPRK4

Gene Description

G protein-coupled receptor kinase 4

Chromosome

Position

2963571-3040760

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Cell SpecificEndometrial glandular cellsBlood Cell SpecificeosinophilBlood Lineage Specificgranulocytes

Function & Pathway8

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component (5)

Molecular Function (4)

Biological Process (3)

KEGG (3)

Reactome (3)

Canonical Pathways (3)

M47 Pid integrin cs pathway
M169 Pid integrin2 pathway
M159 Pid amb2 neutrophils pathway

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence14

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network (4)

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
GRK4	P32298	GNAI1	P63096	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GRK4	P32298	GNAI2	P04899	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GRK4	P32298	GNAI3	P08754	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GRK4	P32298	BKRB2	P30411	Yes	No	Yes	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAHPRDPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteHPRD-phos	ProtMapper:11517230 KEA:11517230 phosphoELM:11517230 HPRD-phos:11517230 SIGNOR:11517230 HPRD:11517230 PhosphoSite:11517230

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	CREB5 GRK4 MAPK14 MAPK7 MEF2C MEF2D UBC	P0CG48 P32298 Q02930 Q06413 Q13164 Q14814 Q16539	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8373
	GRK4	P32298	2	PDB	PDB:4yhj

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	38892056

Sequence, Structure & Domains13

Sequences

Length

578

Mass

66,583

Sequence

MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKEVEPKQC

Alternative Products

Event=Alternative splicing; Named isoforms=4; Name=1; Synonyms=GRK4-alpha, GRK4D; IsoId=P32298-1; Sequence=Displayed; Name=2; Synonyms=GRK4-beta, GRK4C; IsoId=P32298-2; Sequence=VSP_004936; Name=3; Synonyms=GRK4-delta, GRK4A; IsoId=P32298-3; Sequence=VSP_004936, VSP_004937; Name=4; Synonyms=GRK4-gamma, GRK4B; IsoId=P32298-4; Sequence=VSP_004937

Alternative Sequence

18..49; Missing (in isoform 2 and isoform 3); 516..561; Missing (in isoform 3 and isoform 4)

3D Structural Models

Turn

39..42; 95..97; 140..143; 207..209; 277..279; 305..307; 350..352; 433..438; 519..524

Helix

30..33; 43..48; 53..57; 61..71; 75..91; 98..109; 123..132; 144..154; 157..162; 166..178; 184..186; 220..226; 229..241; 272..276; 286..304; 315..317; 355..358; 366..381; 395..403; 415..424; 429..431; 439..444; 447..449; 454..458; 494..500; 509..518

Beta Strand

113..115; 135..137; 187..195; 197..206; 212..219; 250..255; 257..264; 280..283; 318..320; 326..328; 341..343; 363..365; 382..384; 505..507

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Domain (FT)

52..172; RGS; 187..449; Protein kinase; 450..515; AGC-kinase C-terminal

Region

1..154; N-terminal

Protein Families (3)

Protein kinase superfamily
AGC Ser/Thr protein kinase family
GPRK subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Clinical Relevance5

Drugs (2)

VERAPAMIL ATENOLOL

Antibody

HPA057023

Interaction Protein

ENSG00000198055

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications1

PMID	Title	Abstract
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available