Protein detail

GRK5

G protein-coupled receptor kinase 5 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK5)

Entry name GRK5	UniProt ID P34947	EVMP confidence score 0.50
Supporting publications (n) 4	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

G protein-coupled receptor kinase 5 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK5)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000198873

Entrez Gene Symbol

GRK5

Gene Synonym

GPRK5

Gene Description

G protein-coupled receptor kinase 5

Chromosome

Position

119207571-119459745

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization3

Tissue SpecifictongueCell SpecificAdrenal medulla cells

Function & Pathway8

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component (5)

Molecular Function (8)

Biological Process (3)

KEGG (3)

Reactome (3)

Canonical Pathways (3)

M212 Pid integrin5 pathway
M47 Pid integrin cs pathway
M156 Pid ecadherin nascent aj pathway

Mediation Categories

Receptor-signaling mediation

Relations & Evidence38

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
GRK5	MAP3K14	Q99558	S	484	phosphorylation	PhosphoNetworks
GRK5	MAST1	Q9Y2H9	S	484	phosphorylation	PhosphoNetworks
GRK5	SRC	P12931	Y	253	phosphorylation	RLIMS-P_ProtMapperProtMapper	ProtMapper:21097543
GRK5	PRKCB	P05771	T	485	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (14)

14 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
gpcr	receptor	DGIdb	No	Yes	No	No	No
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	No	No
adhesion	adhesion	OmniPath	Yes	Yes	No	No	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	No	No	No

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Regulatory Interaction Network (17)

17 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
GRK5	P34947	GNAI1	P63096	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GRK5	P34947	GNAI2	P04899	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GRK5	P34947	GNAI3	P08754	Yes	No	No	SignaLink3TCRcuration_SignaLink3	SignaLink3:16183910 SignaLink3:10508237 SignaLink3:18538863 SignaLink3:23109003 SignaLink3:15337520 SignaLink3:17084806 SignaLink3:10644702
GRK5	P34947	HDAC5	Q9UQL6	Yes	No	Yes	SIGNOR	SIGNOR:23658733
GRK5	P34947	BKRB2	P30411	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	ProtMapper:11517230 KEA:11517230 HPRD-phos:11517230 SIGNOR:11517230 HPRD:11517230
PGFRB	P09619	GRK5	P34947	Yes	Yes	No	iPTMnetSIGNOR	SIGNOR:19092051
GRK5	P34947	CXCR4	P61073	Yes	Yes	Yes	Sparser_ProtMapperiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperWangREACH_ProtMapper	SIGNOR:24632620 ProtMapper:24632620
GRK5	P34947	SYUB	Q16143	Yes	No	Yes	PhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapper	HPRD:10852916 KEA:10852916 SIGNOR:10852916 ProtMapper:10852916
GRK5	P34947	LRP6	O75581	Yes	Yes	No	NCI-PID_ProtMapperiPTMnetSIGNORProtMapperWang	SIGNOR:2787365 ProtMapper:19801552
GRK5	P34947	ADRB2	P07550	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperHINTPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEAphosphoELMCellinkerSIGNOR_ProtMapper	phosphoELM:8662852 Cellinker:28431242 SIGNOR:8662852 HPRD:8662852 ProtMapper:9516468 HINT:8672451 KEA:96278816 KEA:8662852 ProtMapper:8662852 HINT:8662852

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Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	CALM3 GRK1 GRK5 RCVRN YWHAE YWHAQ	P0DP25 P27348 P34947 P35243 P62258 Q15835	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3991
	GRK5 GRK6	P34947 P43250	0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Protein Organic Solvent Precipitation	Mass spectrometry	1	32384937

Sequence, Structure & Domains13

Sequences

Length

590

Mass

67,787

Sequence

MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS

3D Structural Models

Turn

17..20; 139..142; 206..208; 436..438

Helix

3..16; 22..25; 30..33; 39..42; 43..48; 53..57; 61..71; 75..91; 95..97; 98..109; 123..134; 143..153; 156..162; 165..177; 219..224; 228..240; 271..277; 285..304; 314..316; 330..332; 354..357; 366..380; 394..403; 414..423; 428..430; 439..443; 446..448; 453..457; 477..479; 494..502; 508..517; 520..524; 535..537

Beta Strand

111..113; 185..195; 198..205; 211..218; 249..254; 256..263; 268..270; 278..280; 317..319; 321..323; 325..327; 362..364; 381..383; 384..386; 489..491; 529..531

3D Structure

X-ray crystallography (11)

Domain & Motif Annotations

Compositional Bias

538..547; Basic and acidic residues; 563..590; Low complexity

Motif

388..395; Nuclear localization signal

Domain (FT)

53..171; RGS; 186..448; Protein kinase; 449..514; AGC-kinase C-terminal

Region

1..185; N-terminal; 20..39; Interaction with calmodulin; 531..590; Disordered; 546..565; Sufficient for membrane localization

Protein Families (3)

Protein kinase superfamily
AGC Ser/Thr protein kinase family
GPRK subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Clinical Relevance2

Drugs (15)

TRANDOLAPRIL CITALOPRAM ALISERTIB NULL SOTRASTAURIN NVP-TAE684 MLN-8054 ANTIHYPERTENSIVE AGENT RG-1530 VERAPAMIL ENTRECTINIB HYDROCHLOROTHIAZIDE JNJ-7706621 CHEMBL:CHEMBL1997335 PF-562271

Antibody (2)

CAB025579 HPA046838

Supporting Publications4

PMID	Title	Abstract
26775013	Proteomic characterization of circulating extracellular vesicles identifies novel serum myeloma associated markers.	No abstract available
38490958	Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer.	No abstract available
41019078	Proteomics of plasma-derived extracellular vesicles from human patients identifies biomarkers for monitoring visceral leishmaniasis therapy.	No abstract available
41227296	Extracellular Vesicles Profiling in Acute Myeloid Leukemia Cell Lines: A Proteomic Characterization.	No abstract available