Protein detail
GRK5
G protein-coupled receptor kinase 5 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK5)
Entry name GRK5 | UniProt ID | EVMP confidence score 0.50 |
Supporting publications (n) 4 | Transmembrane count | Protein classification EnzymesPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
G protein-coupled receptor kinase 5 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK5)
Protein Class (2)
EnzymesPredicted intracellular proteins
Protein Function (4)
- ENZYME proteins:Transferases
- Enzymes
- Kinases:AGC Ser/Thr protein kinases
- Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym
GPRK5
Gene Description
G protein-coupled receptor kinase 5
Chromosome
10
Position
119207571-119459745
Supporting publications (n)
4
EVMP confidence score
0.50
Fluorescence & Localization3
Tissue SpecifictongueCell SpecificAdrenal medulla cells
Function & Pathway8
Protein Function (4)
- ENZYME proteins:Transferases
- Enzymes
- Kinases:AGC Ser/Thr protein kinases
- Predicted intracellular proteins
Cellular Component (5)
Molecular Function (8)
- GO:0004672 protein kinase activity
- GO:0004674 protein serine/threonine kinase activity
- GO:0004703 G protein-coupled receptor kinase activity
- GO:0005080 protein kinase C binding
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0005543 phospholipid binding
- GO:0047696 beta-adrenergic receptor kinase activity
Biological Process (3)
KEGG (3)
Reactome (3)
Canonical Pathways (3)
- M212 Pid integrin5 pathway
- M47 Pid integrin cs pathway
- M156 Pid ecadherin nascent aj pathway
Mediation Categories
Receptor-signaling mediation
Relations & Evidence38
Enzyme-Mediated Modification (4)
4 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| GRK5 | MAP3K14 | Q99558 | S | 484 | phosphorylation | PhosphoNetworks | |
| GRK5 | MAST1 | Q9Y2H9 | S | 484 | phosphorylation | PhosphoNetworks | |
| GRK5 | SRC | P12931 | Y | 253 | phosphorylation | RLIMS-P_ProtMapperProtMapper | ProtMapper:21097543 |
| GRK5 | PRKCB | P05771 | T | 485 | phosphorylation | KEA | KEA:17570479 |
Ligand-Receptor Signaling (14)
14 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | Cellinker | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
| receptor | receptor | scConnect | No | Yes | No | No | No |
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Regulatory Interaction Network (17)
17 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| GRK5 | P34947 | P53 | P04637 | Yes | No | Yes | Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperdbPTMSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper | dbPTM:15053879SIGNOR:20124405dbPTM:20124405ProtMapper:27326393ProtMapper:26226012ProtMapper:26029824ProtMapper:20124405 |
| GRK5 | P34947 | NTR1 | P30989 | Yes | Yes | No | SIGNOR | SIGNOR:26120872 |
| GRK5 | P34947 | MCR | P08235 | Yes | No | Yes | SIGNOR | SIGNOR:32326036 |
| GRK5 | P34947 | SYUA | P37840 | Yes | Yes | Yes | HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksNCI-PID_ProtMapperHPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEABioGRIDHPRD_KEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapperSparser_ProtMapperHPRD-phos | HPRD:10852916HPRD-phos:10852916HPRD-phos:10617630ProtMapper:10852916ProtMapper:10617630SIGNOR:16957079KEA:10617630ProtMapper:25915624KEA:10852916ProtMapper:16957079HPRD:10617630KEA:11813001ProtMapper:26614013phosphoELM:10852916BioGRID:10852916 |
| GRK5 | P34947 | F10A1 | P50502 | Yes | Yes | No | iPTMnetSIGNORProtMapperdbPTMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:21728385dbPTM:21728385PhosphoSite:21728385ProtMapper:21728385 |
| GRK5 | P34947 | MOES | P26038 | Yes | No | No | Sparser_ProtMapperiPTMnetProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:24755472ProtMapper:26226012ProtMapper:24755472 |
| GRK5 | P34947 | HDAC6 | Q9UBN7 | Yes | No | No | Sparser_ProtMapperProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:31363957PhosphoSite:32655357ProtMapper:31363957PhosphoSite:30443180 |
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Protein Complex Composition (2)
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Protein Organic Solvent Precipitation | Mass spectrometry | 1 | 32384937 |
Sequence, Structure & Domains13
Sequences
Length
590
Mass
67,787
Sequence
MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS
3D Structural Models
Turn
17..20; 139..142; 206..208; 436..438
Helix
3..16; 22..25; 30..33; 39..42; 43..48; 53..57; 61..71; 75..91; 95..97; 98..109; 123..134; 143..153; 156..162; 165..177; 219..224; 228..240; 271..277; 285..304; 314..316; 330..332; 354..357; 366..380; 394..403; 414..423; 428..430; 439..443; 446..448; 453..457; 477..479; 494..502; 508..517; 520..524; 535..537
Beta Strand
111..113; 185..195; 198..205; 211..218; 249..254; 256..263; 268..270; 278..280; 317..319; 321..323; 325..327; 362..364; 381..383; 384..386; 489..491; 529..531
3D Structure
X-ray crystallography (11)
Domain & Motif Annotations
Compositional Bias
538..547; Basic and acidic residues; 563..590; Low complexity
Motif
388..395; Nuclear localization signal
Domain (FT)
53..171; RGS; 186..448; Protein kinase; 449..514; AGC-kinase C-terminal
Region
1..185; N-terminal; 20..39; Interaction with calmodulin; 531..590; Disordered; 546..565; Sufficient for membrane localization
Protein Families (3)
- Protein kinase superfamily
- AGC Ser/Thr protein kinase family
- GPRK subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Clinical Relevance2
Supporting Publications4
| PMID | Title | Abstract |
|---|---|---|
| 26775013 | Proteomic characterization of circulating extracellular vesicles identifies novel serum myeloma associated markers. | No abstract available |
| 38490958 | Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer. | No abstract available |
| 41019078 | Proteomics of plasma-derived extracellular vesicles from human patients identifies biomarkers for monitoring visceral leishmaniasis therapy. | No abstract available |
| 41227296 | Extracellular Vesicles Profiling in Acute Myeloid Leukemia Cell Lines: A Proteomic Characterization. | No abstract available |