EV MemProt Atlas

Basic Information11

Protein Names

Radixin

Protein Class (3)

Disease related genesHuman disease related genesPredicted intracellular proteins

Protein Function (3)

Disease related genes
Predicted intracellular proteins
Human disease related genes:Nervous system diseases:Ear disease

Ensembl

ENSG00000137710

Entrez Gene Symbol

RDX

Gene Synonym

DFNB24

Gene Description

Radixin

Chromosome

11

Position

109864295-110296712

Supporting publications (n)

13

EVMP confidence score

0.72

Fluorescence & Localization5

Tissue SpecificesophagusCell SpecificCytotrophoblastsSingle-Nuclei Brain Specificcentral nervous system macrophageSecretome LocationSecreted to bloodSecretome FunctionEnzyme inhibitor

Function & Pathway7

Protein Function (3)

Disease related genes
Predicted intracellular proteins
Human disease related genes:Nervous system diseases:Ear disease

Cellular Component (17)

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Molecular Function (7)

Biological Process (3)

KEGG (5)

Reactome (6)

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence77

Enzyme-Mediated Modification (13)

13 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
RDX	ROCK2	O75116	T	564	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA	KEA:9456324
RDX	ROCK2	O75116	T	573	phosphorylation	MIMPHPRD_MIMPKEAphosphoELM_MIMP	KEA:9456324
RDX	PRKCA	P17252	T	564	phosphorylation	KEA	KEA:17570479

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Ligand-Receptor Signaling (13)

13 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
ferm_domain	intracellular_intercellular_related	HGNC	Yes	No	No	No	No
adhesion_cytoskeleton_adaptor	intracellular_intercellular_related	Adhesome	Yes	No	No	No	No

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Regulatory Interaction Network (4)

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ROCK1	Q13464	RADI	P35241	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAHPRDKinexus_KEAWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEASIGNOR_ProtMapperAdhesomeACSNHPRD-phos	SIGNOR:9456324 HPRD-phos:9456324 KEA:17447891 KEA:9456324 Adhesome:9456324 ACSN:10970850 ACSN:10047517 HPRD:9456324 ProtMapper:20068231 HPRD-phos:20068231 ACSN:8522586 ProtMapper:9456324 ACSN:8527459 ACSN:9287351 ACSN:12778124 ACSN:12802084 ACSN:12045227 ACSN:7579708
LRRK2	Q5S007	RADI	P35241	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperKEAKinexus_KEAPhosphoSite_ProtMapper	KEA:17447891 SIGNOR:17447891
RHOA	P61586	RADI	P35241	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	ProtMapper:35267019 SIGNOR:35267019
VPS11	Q9H270	RADI	P35241	Yes	Yes	No	SIGNOR	SIGNOR:21148287

Protein Complex Composition (46)

46 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ALG12 H2AX PRDX2 PRDX3 PRDX4 SUMO4 UBC	P0CG48 P16104 P30048 P32119 Q13162 Q6EEV6 Q9BV10	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8857
DUSP23 H2AX PRDX2 PRDX3 PRDX4 SUMO4 UBC	P0CG48 P16104 P30048 P32119 Q13162 Q6EEV6 Q9BVJ7	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4181
ACSM2B PRDX2 PRDX3 PRDX4 SUMO4 UBC	P0CG48 P30048 P32119 Q13162 Q68CK6 Q6EEV6	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4269
ACLY PKM PRDX2	P14618 P32119 P53396	0:0:0	hu.MAP2
DUSP23 H2AC20 H2AX H2BC14 H3C11 H4C4 KAT5 NAP1L5 PRDX4 RCC1 SSRP1 SUPT16H	P16104 P18754 P62805 P68431 Q08945 Q13162 Q16777 Q92993 Q96NT1 Q99879 Q9BVJ7 Q9Y5B9	1:1:2:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7578
DTYMK PRDX1 PRDX2 TTN	P23919 P32119 Q06830 Q8WZ42	0:0:0:0	hu.MAP
PRDX5	P30044	8	PDB	PDB:2vl9PDB:1h4oPDB:2vl3PDB:1oc3PDB:2vl2PDB:4k7oPDB:4k7iPDB:4k7nPDB:4mmm
PRDX5 SPR	P30044 P35270	0:0	hu.MAP
IFT27 PRDX5 PTGR1 SUMO1	P30044 P63165 Q14914 Q9BW83	0:0:0:0	Havugimana2012	Havugimana2012:C_303
BOLA3 C11orf54 FAHD2A FAHD2B PRDX5	P30044 Q53S33 Q6P2I3 Q96GK7 Q9H0W9	0:0:0:0:0	hu.MAP2

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	37968711

Sequence, Structure & Domains9

Sequences

Length

583

Mass

68,564

Sequence

MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVNYFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERAQLENEKKKREIAEKEKERIEREKEELMERLKQIEEQTIKAQKELEEQTRKALELDQERKRAKEEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQEDLEKTKEELKTVMSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKKQLQALSSELAQARDETKKTQNDVLHAENVKAGRDKYKTLRQIRQGNTKQRIDEFEAM

Alternative Products

Event=Alternative splicing; Named isoforms=5; Name=1; IsoId=P35241-1; Sequence=Displayed; Name=2; IsoId=P35241-2; Sequence=VSP_045315, VSP_045318; Name=3; IsoId=P35241-3; Sequence=VSP_045316, VSP_045317, VSP_045318; Name=4; IsoId=P35241-4; Sequence=VSP_047276; Name=5; IsoId=P35241-5; Sequence=VSP_045318

Alternative Sequence

1..347; Missing (in isoform 2); 1..156; MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQR -> MLSWNLPFSPIQLANNFLTS (in isoform 4); 33..64; Missing (in isoform 3); 168..539; Missing (in isoform 3); 583; M -> MWGPKLYALFQMRSCQSSIKQM (in isoform 2, isoform 3 and isoform 5)

Domain & Motif Annotations

Compositional Bias

376..400; Basic and acidic residues; 469..480; Pro residues; 483..492; Basic and acidic residues; 506..525; Basic and acidic residues

Domain (CC)

The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ability to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN (By similarity).

Domain (FT)

5..295; FERM

Region

310..330; Disordered; 376..407; Disordered; 462..526; Disordered

Clinical Relevance5

Disease Involvement (3)

DeafnessDisease variantNon-syndromic deafness

Antibody (3)

HPA000263 HPA000763 CAB037312

Interaction Protein (2)

ENSG00000092820ENSG00000141522

Interaction Count

2

Interaction Dataset (2)

intact_biogrid_bioplexintact_biogrid

Supporting Publications13

PMID	Title	Abstract
27605433	Secreted primary human malignant mesothelioma exosome signature reflects oncogenic cargo.	No abstract available
27863537	High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources.	No abstract available
27894104	Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.	No abstract available
28590090	Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.	No abstract available
28986585	Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.	No abstract available
29687628	Proteomic Profiling Exosomes from Vascular Smooth Muscle Cell.	No abstract available
30646616	Preferential Localization of MUC1 Glycoprotein in Exosomes Secreted by Non-Small Cell Lung Carcinoma Cells.	THBS1, ANXA6, HIST1H4A, COL18A1, MDK, SRGN, ENO1, TUBA4A, SLC3A2, GPI, MIF, MUC1, TALDO1, SLC7A5, ICAM1, HSP90AA1, G6PD, and LRP1 were found to be expressed in exosomes at more than 5-fold higher level as compared to total cellular membrane proteins.
32089743	Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism.	No abstract available
32284825	Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine.	No abstract available
33035814	Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells.	No abstract available