EV MemProt Atlas

Basic Information11

Protein Names

Myosin-9 (Cellular myosin heavy chain, type A) (Myosin heavy chain 9) (Myosin heavy chain, non-muscle IIa) (Non-muscle myosin heavy chain A) (NMMHC-A) (Non-muscle myosin heavy chain IIa) (NMMHC II-a) (NMMHC-IIA)

Protein Class (5)

Cancer-related genesDisease related genesHuman disease related genesPlasma proteinsPredicted intracellular proteins

Protein Function (5)

Cancer-related genes:Mutational cancer driver genes
Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins
Disease related genes
Human disease related genes:Nervous system diseases:Ear disease

Ensembl

ENSG00000100345

Entrez Gene Symbol

MYH9

Gene Synonym (6)

DFNA17EPSTSFTNSMHANMHC-II-ANMMHCA

Gene Description

Myosin heavy chain 9

Chromosome

22

Position

36281280-36388010

Supporting publications (n)

52

EVMP confidence score

0.88

Fluorescence & Localization1

Function & Pathway8

Protein Function (5)

Cancer-related genes:Mutational cancer driver genes
Human disease related genes:Cardiovascular diseases:Hematologic diseases
Predicted intracellular proteins
Disease related genes
Human disease related genes:Nervous system diseases:Ear disease

Cellular Component (28)

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Molecular Function (15)

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Biological Process (3)

KEGG (8)

Reactome (27)

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Canonical Pathways

M277 Pid integrin a4b1 pathway

Mediation Categories (6)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence43

Enzyme-Mediated Modification (14)

14 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database
MYH9	CSNK2B	P67870	S	1,916	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
MYH9	PRKCG	P05129	S	1,916	phosphorylation	MIMPphosphoELM_MIMPPhosphoSite_MIMP
MYH9	PRKCD	Q05655	S	1,916	phosphorylation	MIMPphosphoELM_MIMPPhosphoSite_MIMP
MYH9	PRKCE	Q02156	S	1,916	phosphorylation	MIMPphosphoELM_MIMPPhosphoSite_MIMP

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Ligand-Receptor Signaling (11)

11 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	GO_Intercell	No	Yes	No	No	No
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
actin_regulation_adhesome	intracellular_intercellular_related	Adhesome	Yes	No	No	No	No
intracellular_intercellular_related	intracellular_intercellular_related	OmniPath	Yes	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No

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Regulatory Interaction Network (8)

8 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
CSK21	P68400	MYH9	P35579	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:19826041 PhosphoSite:22123909 SIGNOR:18971378 ProtMapper:21316371 ProtMapper:18971378 PhosphoSite:18971378 PhosphoSite:30087119 SIGNOR:22123909 PhosphoSite:24462114 ProtMapper:17567956 ProtMapper:22123909 SIGNOR:21316371 PhosphoSite:30267961 SIGNOR:17567956
SPC25	Q9HBM1	MYH9	P35579	Yes	Yes	No	SIGNOR	SIGNOR:39488790
RIOK1	Q9BRS2	MYH9	P35579	Yes	Yes	No	SIGNOR	SIGNOR:39488790
RHG07	Q96QB1	MYH9	P35579	Yes	No	Yes	SIGNOR	SIGNOR:26977077
MYH9	P35579		COMPLEX:O15514_P0CAP2_P19387_P19388_P24928_P30876_P36954_P52434_P52435_P53803_P61218_P62487_P62875_Q6EEV4_Q9GZM3_Q9H1A7	Yes	Yes	No	SIGNOR	SIGNOR:32685004
S10A4	P26447	MYH9	P35579	Yes	No	Yes	SIGNORHPRDHINTBioGRIDIntActDIP	DIP:22483112 HINT:20421509 IntAct:20421509 IntAct:22483112 BioGRID:37509135 HINT:22460785 SIGNOR:16707441 DIP:20421509 HPRD:9405067 IntAct:31837246 HPRD:15289939 IntAct:22460785 HINT:33961781 HINT:22483112 HINT:24830809
KPCA	P17252	MYH9	P35579	Yes	No	No	BEL-Large-Corpus_ProtMapperPhosphoNetworksphosphoELM_MIMPAdhesomeMIMPPhosphoSite_MIMPPhosphoSite_ProtMapperiPTMnetProtMapperPhosphoSite_KEAKinexus_KEAKEAphosphoELM_KEAphosphoELMPhosphoSiteNetworKIN_KEA	phosphoELM:9545325 PhosphoSite:25544611 PhosphoSite:17567956 ProtMapper:15212693 KEA:9545325 PhosphoSite:9545325 KEA:91113708 PhosphoSite:1899200 Adhesome:9545325 PhosphoSite:29208598 Adhesome:91113708 KEA:1899200 KEA:17570479 phosphoELM:1899200 Adhesome:1899200 PhosphoSite:21316371
SRC	P12931	MYH9	P35579	Yes	No	No	iPTMnetPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:25635050

Protein Complex Composition (9)

9 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ACO2 ACTB ACTN2 CKM CLNS1A CS GCN1 H1-5 HSPB6 MYH2 MYH9 TNNI3 TNNT2 TPM1 ZFP36	O14558 O75390 P06732 P09493 P16401 P19429 P26651 P35579 P35609 P45379 P54105 P60709 Q92616 Q99798 Q9UKX2	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6448
ACTRT1 MYH10 MYH9 MYL12B MYL6 MYO18A PLEC	O14950 O95411 P35579 P35580 P60660 Q15149 Q8TDG2	0:0:0:0:0:0:0	hu.MAP2
BECN1 MYH10 MYH9 MYL12B MYL6 USP45	O14950 P35579 P35580 P60660 Q14457 Q70EL2	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8563
ACTRT1 MYH10 MYH9 MYL12B MYL6 MYO18A PLEC	O14950 P35579 P35580 P60660 Q15149 Q8TDG2 Q92614	0:0:0:0:0:0:0	hu.MAP2
ACO2 ACTB ACTN2 CKM CS GCN1 H1-5 MYH9 NDUFB10 NTPCR SLC25A3 TNNI3 TNNT2 TPM1 ZFP36	O75390 O96000 P06732 P09493 P16401 P19429 P26651 P35579 P35609 P45379 P60709 Q00325 Q92616 Q99798 Q9BSD7	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7939
ACO2 ACTB ACTN2 CKM CS GCN1 H1-5 MAPRE3 MYH2 MYH9 TNNC1 TNNI3 TNNT2 TPM1 ZFP36	O75390 P06732 P09493 P16401 P19429 P26651 P35579 P35609 P45379 P60709 P63316 Q92616 Q99798 Q9UKX2 Q9UPY8	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7155
ACTB CALM3 CORO1A MCM5 MYH9 MYL6 MYO19 PWP1 UBC UNC45A	P0CG48 P0DP25 P31146 P33992 P35579 P60660 P60709 Q13610 Q96H55 Q9H3U1	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8287
MYH9 S100A4	P26447 P35579	2:1	PDB	PDB:4etoPDB:4cfqPDB:3zwhPDB:2lnkPDB:4cfr
MYH10 MYH9	P35579 P35580	0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	37886648

Sequence, Structure & Domains15

Sequences

Length

1,960

Mass

226,532

Sequence

MAQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEEEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVMQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLKLRNWQWWRLFTKVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEEQIILEDQNCKLAKEKKLLEDRIAEFTTNLTEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELESQISELQEDLESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVELDNVTGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQVEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVGCLETAEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSACNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAVAARKKLEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEKKLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P35579-1; Sequence=Displayed; Name=2; IsoId=P35579-2; Sequence=VSP_035409, VSP_035410

Alternative Sequence

1..136; Missing (in isoform 2); 980..1421; Missing (in isoform 2)

3D Structural Models

Helix

1899..1903; 1904..1921

Beta Strand

1895..1897; 1922..1925

3D Structure

NMR spectroscopy (1); X-ray crystallography (4)

Domain & Motif Annotations

Compositional Bias

1035..1055; Basic and acidic residues; 1122..1137; Basic and acidic residues; 1948..1960; Basic and acidic residues

Coiled Coil

837..1926

Domain (CC)

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Domain (FT)

27..77; Myosin N-terminal SH3-like; 81..776; Myosin motor; 779..808; IQ

Region

2..838; Mediates interaction with LIMCH1; 654..676; Actin-binding; 1035..1057; Disordered; 1118..1137; Disordered; 1877..1960; Disordered

Protein Families (2)

TRAFAC class myosin-kinesin ATPase superfamily
Myosin family

Sequence Similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Clinical Relevance5

Disease Involvement (6)

Alport syndromeCancer-related genesCataractDeafnessDisease variantNon-syndromic deafness

Antibody (3)

HPA001644 CAB015386 HPA064783

Interaction Protein (10)

ENSG00000077549ENSG00000092841ENSG00000101335ENSG00000101608ENSG00000118680ENSG00000133895ENSG00000144381ENSG00000177885ENSG00000196154ENSG00000196465

Interaction Count

10

Interaction Dataset (4)

biogrid_opencellintact_biogrid_opencellbiogrid_opencell_bioplexintact_biogrid

Supporting Publications50

PMID	Title	Abstract
29687628	Proteomic Profiling Exosomes from Vascular Smooth Muscle Cell.	No abstract available
29740045	Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy.	Untargeted proteomics detected markers of exosomes (CD9, CD63, CD81), immune activation (CD14, CRP, HLA-A, HLA-B), oxidative stress (CAT, PRDX1, PRDX2, TXN), and Notch4 in plasma exosomes.
30379855	Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis.	No abstract available
30408591	Portrait of blood-derived extracellular vesicles in patients with Parkinson's disease.	No abstract available
30646616	Preferential Localization of MUC1 Glycoprotein in Exosomes Secreted by Non-Small Cell Lung Carcinoma Cells.	THBS1, ANXA6, HIST1H4A, COL18A1, MDK, SRGN, ENO1, TUBA4A, SLC3A2, GPI, MIF, MUC1, TALDO1, SLC7A5, ICAM1, HSP90AA1, G6PD, and LRP1 were found to be expressed in exosomes at more than 5-fold higher level as compared to total cellular membrane proteins.
30950185	Unique Protein Profiles of Extracellular Vesicles as Diagnostic Biomarkers for Early and Advanced Non-Small Cell Lung Cancer.	No abstract available
31081149	Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosis.	No abstract available
31196968	Cancer Cell Derived Small Extracellular Vesicles Contribute to Recipient Cell Metastasis Through Promoting HGF/c-Met Pathway.	No abstract available
31941606	The role of actinin-4 (ACTN4) in exosomes as a potential novel therapeutic target in castration-resistant prostate cancer.	No abstract available
32089743	Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism.	No abstract available

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Entry name MYH9	UniProt ID P35579	EVMP confidence score 0.88
Supporting publications (n) 52	Transmembrane count	Protein classification Cancer-related genesDisease related genesHuman disease related genesPlasma proteinsPredicted intracellular proteins