EV MemProt Atlas

Basic Information10

Protein Names

Tyrosine-protein kinase SYK (EC 2.7.10.2) (Spleen tyrosine kinase) (p72-Syk)

Protein Class (6)

Cancer-related genesDisease related genesEnzymesFDA approved drug targetsHuman disease related genesPredicted intracellular proteins

Protein Function (8)

Predicted intracellular proteins
ENZYME proteins:Transferases
Human disease related genes:Immune system diseases:Primary immunodeficiency
Enzymes
Cancer-related genes
Kinases:Tyr protein kinases
Disease related genes
FDA approved drug targets:Small molecule drugs

Ensembl

ENSG00000165025

Entrez Gene Symbol

SYK

Gene Description

Spleen associated tyrosine kinase

Chromosome

9

Position

90801787-90898549

Supporting publications (n)

4

EVMP confidence score

0.50

Fluorescence & Localization2

Cell SpecificPlateletsSingle-Nuclei Brain Specificendothelial cell

Function & Pathway7

Protein Function (8)

Predicted intracellular proteins
ENZYME proteins:Transferases
Human disease related genes:Immune system diseases:Primary immunodeficiency
Enzymes
Cancer-related genes
Kinases:Tyr protein kinases
Disease related genes
FDA approved drug targets:Small molecule drugs

Cellular Component (8)

Molecular Function (17)

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Biological Process (3)

KEGG (17)

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Reactome (39)

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Mediation Categories (6)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence101

Enzyme-Mediated Modification (29)

29 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
SYK	SRC	P12931	Y	352	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:23100514
SYK	PRKAA1	Q13131	S	178	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
SYK	TLR9	Q9NR96	Y	525	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:27258042
SYK	TLR9	Q9NR96	Y	526	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:27258042
SYK	TLR9	Q9NR96	Y	352	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:27258042
SYK	CD36	P16671	Y	525	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:23395392
SYK	CD36	P16671	Y	526	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:23395392
SYK	ABL1	P00519	Y	525	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:23100514
SYK	ABL1	P00519	Y	526	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:23100514
SYK	ABL1	P00519	Y	352	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:23100514

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Ligand-Receptor Signaling (13)

13 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	No	No
adhesion	adhesion	OmniPath	Yes	Yes	No	No	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No

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Regulatory Interaction Network (56)

56 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
FGR	P09769	KSYK	P43405	Yes	Yes	No	DOMINOWangPhosphoPointSIGNORHPRDBioGRIDSPIKE_LCLit-BM-17	Lit-BM-17:15536084 DOMINO:15536084 BioGRID:11672534 SIGNOR:21746961 SPIKE_LC:15536084 HPRD:11672534 Lit-BM-17:11672534
ABL1	P00519	KSYK	P43405	Yes	Yes	No	REACH_ProtMapperSIGNORProtMapperAdhesome	Adhesome:7797565 ProtMapper:23100514 SIGNOR:23100514
KSYK	P43405	UBP25	Q9UHP3	Yes	No	Yes	Sparser_ProtMapperSIGNORProtMapperREACH_ProtMapperHINTHuRIIntActLit-BM-17	HINT:19909739 IntAct:25416956 IntAct:32296183 SIGNOR:19909739 ProtMapper:19909739 HINT:32707033 ProtMapper:34177595 IntAct:21516116 Lit-BM-17:19909739
KSYK	P43405	IKZF1	Q13422	Yes	Yes	No	PhosphoSite_MIMPMIMPPhosphoSite_norefSIGNORiPTMnetProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:23071339 SIGNOR:23071339 ProtMapper:23071339
KSYK	P43405	PAX5	Q02548	Yes	No	Yes	SIGNOR	SIGNOR:27181361
KSYK	P43405	CGAS	Q8N884	Yes	Yes	No	SIGNOR	SIGNOR:36252040
SHIP1	Q92835	KSYK	P43405	Yes	No	Yes	SIGNORAdhesome	Adhesome:22267732 SIGNOR:32323266
KSYK	P43405	NTAL	Q9GZY6	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperWangPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:17993265 PhosphoSite:14722116 SIGNOR:14722116
KSYK	P43405	B3AT	P02730	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSiteSparser_ProtMapperHPRD-phos	HPRD:16118313 PhosphoSite:21951684 HPRD-phos:2065070 ProtMapper:1998697 HPRD-phos:10942405 PhosphoSite:15649129 phosphoELM:10942405 PhosphoSite:1998697 PhosphoSite:12070037 KEA:10942405 PhosphoSite:17404032 HPRD:2065070 HPRD:1998697 PhosphoSite:2065070 PhosphoSite:27856737 ProtMapper:2065070 PhosphoSite:18827007 ProtMapper:10942405 KEA:1998697 PhosphoSite:10942405 ProtMapper:19306898 SIGNOR:10942405 PhosphoSite:6681814 KEA:2065070 HPRD:10942405
NEDD4	P46934	KSYK	P43405	Yes	No	Yes	SIGNOR	SIGNOR:11046148

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Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	CBL SYK	P22681 P43405	2:2	PDB	PDB:3buw
	SYK	P43405	2	PDB	PDB:4xg8PDB:8xq0PDB:3srvPDB:3fqhPDB:7q63PDB:5ghvPDB:8xq2PDB:8wywPDB:8xq1PDB:4xg9PDB:4xg3PDB:5t68PDB:7sa7PDB:5lmbPDB:5y5uPDB:4puzPDB:6vov

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass Spectrometry	1	38113368

Sequence, Structure & Domains14

Sequences

Length

635

Mass

72,066

Sequence

MASSGMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=Long; IsoId=P43405-1; Sequence=Displayed; Name=Short; IsoId=P43405-2; Sequence=VSP_005010

Alternative Sequence

283..305; Missing (in isoform Short)

3D Structural Models

Turn

567..569; 572..575

Helix

10..12; 22..31; 85..92; 119..136; 140..158; 163..165; 175..183; 237..244; 342..344; 346..348; 351..353; 360..363; 367..369; 411..424; 456..462; 468..487; 497..499; 513..515; 536..538; 541..546; 551..566; 578..586; 599..608; 613..615; 619..624

Beta Strand

38..43; 47..49; 51..57; 60..68; 74..76; 82..84; 99..101; 169..172; 184..186; 187..189; 192..201; 203..209; 212..220; 221..224; 226..228; 229..231; 234..236; 251..253; 370..372; 377..380; 384..391; 393..409; 435..449; 500..504; 507..510; 524..527; 548..550; 589..591

3D Structure

NMR spectroscopy (2); X-ray crystallography (91)

Domain & Motif Annotations

Domain (CC)

The SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins. Some proteins like CLEC1B have a partial ITAM domain (also called hemITAM) containing a single YxxL motif. The interaction with SYK requires CLEC1B homodimerization.

Domain (FT)

15..107; SH2 1; 168..259; SH2 2; 371..631; Protein kinase

Region

108..167; Interdomain A; 260..370; Interdomain B

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
SYK/ZAP-70 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.

Clinical Relevance9

Disease Involvement (3)

Cancer-related genesDisease variantFDA approved drug targets

Related Diseases (17)

Acquired hypomelanotic disorder Acute myeloid leukaemia Allergic/hypersensitivity disorder Asthma Atopic eczema B-cell lymphoma Chronic obstructive pulmonary disease Cutaneous lupus erythematosus Diabetes mellitus Diffuse large B-cell lymphoma Malignant haematopoietic neoplasm Mature B-cell leukaemia Rheumatoid arthritis Solid tumour/cancer Thrombocytopenia Thrombosis Urticaria

Biomarker

Phase 1; Phase 2; Investigative; Phase 3; Approved

Drug Targets

FDA approved drug targets

Antibody (2)

HPA001384 CAB007773

Interaction Protein (8)

ENSG00000110395ENSG00000112773ENSG00000145675ENSG00000146648ENSG00000154127ENSG00000155313ENSG00000158869ENSG00000182866

Interaction Count

8

Interaction Dataset

intact_biogrid

Supporting Publications4

PMID	Title	Abstract
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available
40091455	Potential Role of Menstrual Fluid-Derived Small Extracellular Vesicle Proteins in Endometriosis Pathogenesiss.	No abstract available
40098346	Toward Identification of Markers for Brain-Derived Extracellular Vesicles in Cerebrospinal Fluid: A Large-Scale, Unbiased Analysis Using Proximity Extension Assays.	No abstract available
41227296	Extracellular Vesicles Profiling in Acute Myeloid Leukemia Cell Lines: A Proteomic Characterization.	No abstract available

Entry name KSYK	UniProt ID P43405	EVMP confidence score 0.50
Supporting publications (n) 4	Transmembrane count	Protein classification Cancer-related genesDisease related genesEnzymesFDA approved drug targetsHuman disease related genesPredicted intracellular proteins

KSYK