Protein detail
EAA4
Excitatory amino acid transporter 4 (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6)
Protein symbol EAA4 | UniProt ID | EVMP score 0.50 |
Frequency 2 | Transmembrane count 8 | Protein classification Metabolic proteinsPredicted intracellular proteinsPredicted membrane proteinsTransporters |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Excitatory amino acid transporter 4 (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6)
Protein Class
Metabolic proteinsPredicted intracellular proteinsPredicted membrane proteinsTransporters
Protein Function
- Transporters:Electrochemical Potential-driven transporters
- Predicted intracellular proteins
Transmembrane
56..76; Helical; 99..119; Helical; 133..153; Helical; 262..285; Helical; Name=4; 295..322; Helical; Name=5; 344..365; Helical; Name=6; 411..437; Helical; Name=7; 498..519; Helical; Name=8
Transmembrane Count
8
Ensembl
Entrez Gene Symbol
Gene Synonym
EAAT4
Gene Description
Solute carrier family 1 member 6
Chromosome
19
Position
14950033-15022990
Frequency
2
EVMP Score
0.50
Fluorescence & Localization
Blood Cell SpecificbasophilBlood Lineage Specificgranulocytes
Function & Pathway
Protein Function
- Transporters:Electrochemical Potential-driven transporters
- Predicted intracellular proteins
Cellular Component
Molecular Function
- GO:0005313 L-glutamate transmembrane transporter activity
- GO:0005314 high-affinity L-glutamate transmembrane transporter activity
- GO:0015175 neutral L-amino acid transmembrane transporter activity
- GO:0015183 L-aspartate transmembrane transporter activity
- GO:0015501 glutamate:sodium symporter activity
- GO:0046872 metal ion binding
KEGG
Reactome
- R-hsa-210500 glutamate neurotransmitter release cycle
- R-hsa-112316 neuronal system
- R-hsa-112310 neurotransmitter release cycle
- R-hsa-425407 slc mediated transmembrane transport
- R-hsa-9958863 slc mediated transport of amino acids
- R-hsa-112315 transmission across chemical synapses
- R-hsa-382551 transport of small molecules
Mediation Categories
Fusion and delivery mediation
Relations & Evidence
Enzyme-Mediated Modification
0 records.
Ligand-Receptor Signaling
26 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | No | Yes | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | CellPhoneDB | Yes | No | No | No | No |
| cell_surface_ligand | cell_surface_ligand | OmniPath | Yes | No | No | No | No |
| transporter | transporter | Surfaceome | No | Yes | No | No | No |
| solute_carrier | transporter | Almen2009 | No | Yes | No | No | No |
| slc1 | transporter | Surfaceome | No | Yes | No | No | No |
| slc | transporter | Surfaceome | No | Yes | No | No | No |
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Regulatory Interaction Network
1 record.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| CAV1 | Q03135 | EAA4 | P48664 | Yes | No | Yes | SIGNOR | SIGNOR:26690923 |
Protein Complex Composition
2 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| Glutamate_byGLS2_and_SLC1A6 | GLS2SLC1A6 | P48664Q9UI32 | 1:1 | CellPhoneDBCellChatDB | CellPhoneDB:Glutamate_byGLS2_and_SLC1A6 | |
| Glutamate_byGLS_and_SLC1A6 | GLSSLC1A6 | O94925P48664 | 1:1 | CellPhoneDBCellChatDB | CellPhoneDB:Glutamate_byGLS_and_SLC1A6 |
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyPolymer Precipitation | Mass spectrometryR Sequencing | 34 | 23161513283965112316151332988874327954142316151327605433395581342316151330408591405634753095018532056672322848253234135735611462346239183976615829436780363985643778691838168906407486583481790628986585278941043064661641197617383215354008906732089743337213743370951034980157 |
Sequence, Structure & Domains
Sequences
Length
564
Mass
61,565
Sequence
MSSHGNSLFLRESGQRLGRVGWLQRLQESLQQRALRTRLRLQTMTLEHVLRFLRRNAFILLTVSAVVIGVSLAFALRPYQLTYRQIKYFSFPGELLMRMLQMLVLPLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTIIAVFIGILMVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRTMVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGLVVFSVAFGLVIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIWYAPVGILFLIAGKILEMEDMAVLGGQLGMYTLTVIVGLFLHAGIVLPLIYFLVTHRNPFPFIGGMLQALITAMGTSSSSATLPITFRCLEEGLGVDRRITRFVLPVGATVNMDGTALYEALAAIFIAQVNNYELNLGQITTISITATAASVGAAGIPQAGLVTMVIVLTSVGLPTEDITLIIAVDWFLDRLRTMTNVLGDSIGAAVIEHLSQRELELQEAELTLPSLGKPYKSLMAQEKGASRGRGGNESAM
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P48664-1; Sequence=Displayed; Name=2; IsoId=P48664-2; Sequence=VSP_055130
Alternative Sequence
313..564; Missing (in isoform 2)
3D Structural Models
Domain & Motif Annotations
Domain (CC)
Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.
Protein Families
- Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
- SLC1A6 subfamily
Sequence Similarities
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A6 subfamily.