EV MemProt Atlas

Basic Information11

Protein Names

Protein numb homolog (h-Numb) (Protein S171)

Protein Class (2)

Cancer-related genesPredicted intracellular proteins

Protein Function (2)

Cancer-related genes:Candidate cancer biomarkers
Predicted intracellular proteins

Ensembl

ENSG00000133961

Entrez Gene Symbol

NUMB

Gene Synonym

C14orf41

Gene Description

NUMB endocytic adaptor protein

Chromosome

14

Position

73275107-73458617

Supporting publications (n)

13

EVMP confidence score

0.60

Fluorescence & Localization2

Cell SpecificMyonuclei

Function & Pathway7

Protein Function (2)

Cancer-related genes:Candidate cancer biomarkers
Predicted intracellular proteins

Cellular Component (11)

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Molecular Function (4)

Biological Process (3)

KEGG

hsa04330 Notch signaling pathway

Reactome (13)

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Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence38

Enzyme-Mediated Modification (11)

11 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
NUMB	CDK5	Q00535	S	288	phosphorylation	RLIMS-P_ProtMapperREACH_ProtMapperProtMapper	ProtMapper:25403733

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Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (15)

15 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
NUMB	P49757	NOTC1	P46531	Yes	Yes	Yes	WangNetPathSIGNORHPRDCui2007HINTBioGRIDSPIKE_LCSPIKE	SPIKE:16713569 HPRD:12682059 SPIKE_LC:8755477 HINT:8755477 HPRD:8755477 NetPath:8755477 SPIKE:15358736 SPIKE_LC:15358736 SPIKE:8755477 SPIKE_LC:16713569 BioGRID:8755477 HINT:23252402 SIGNOR:12682059
NUMB	P49757	ITCH	Q96J02	Yes	Yes	No	WangNetPathSIGNORHPRDCui2007HINTSignaLink3SPIKE_LCSPIKE	HINT:20818436 HPRD:12682059 SignaLink3:20818436 SPIKE:12682059 SPIKE_LC:12682059 SignaLink3:18628966 HINT:12682059 SIGNOR:20818436 SignaLink3:23331499 HINT:18628966 SignaLink3:12682059 NetPath:12682059
AAK1	Q2M2I8	NUMB	P49757	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:18657069 SIGNOR:18657069 phosphoELM:18657069 ProtMapper:18657069
NUMB	P49757	MDM2	Q00987	Yes	No	Yes	SIGNORHPRDHINTBioGRIDIntActDIPLit-BM-17	Lit-BM-17:28223335 SIGNOR:20940030 BioGRID:22337874 HINT:18172499 DIP:18172499 Lit-BM-17:27106262 HINT:22337874 BioGRID:31451549 HPRD:9632782 Lit-BM-17:23252402 HINT:12646252 Lit-BM-17:18172499 Lit-BM-17:16678796 HINT:29269425 Lit-BM-17:22337874 IntAct:18172499 HINT:16678796 Lit-BM-17:12646252 HPRD:12646252
MDM2	Q00987	NUMB	P49757	Yes	No	Yes	SIGNORHPRDHINTBioGRIDIntActDIPLit-BM-17	Lit-BM-17:28223335 BioGRID:22337874 HINT:18172499 DIP:18172499 Lit-BM-17:27106262 HINT:22337874 BioGRID:31451549 HPRD:9632782 Lit-BM-17:23252402 HINT:12646252 SIGNOR:12646252 Lit-BM-17:18172499 Lit-BM-17:16678796 HINT:29269425 Lit-BM-17:22337874 IntAct:18172499 HINT:16678796 Lit-BM-17:12646252 HPRD:12646252
LNX1	Q8TBB1	NUMB	P49757	Yes	No	Yes	SIGNORELMHINTHPRDBioGRIDSPIKE_LC	HINT:11782429 BioGRID:16002321 ELM:9535908 HINT:16002321 HPRD:11782429 SIGNOR:11782429 ELM:9846878 SPIKE_LC:16713569 HINT:14990566 HPRD:9535908
ZNT9	Q6PML9	NUMB	P49757	Yes	No	Yes	SIGNOR	SIGNOR:11782429
NUMB	P49757	GLI1	P08151	Yes	No	Yes	SIGNORACSN	ACSN:18552861 ACSN:20818436 ACSN:20940030 ACSN:17115028 SIGNOR:20818436 ACSN:17312394
LNX2	Q8N448	NUMB	P49757	Yes	No	Yes	HPRDHINTLit-BM-17SIGNOR	HPRD:11922143 SIGNOR:26451611 HPRD:11782429 HINT:26451611 SIGNOR:32714586 Lit-BM-17:26451611 Lit-BM-17:25814554
KCC1A	Q14012	NUMB	P49757	Yes	No	Yes	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	ProtMapper:17022975 SIGNOR:17022975

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Protein Complex Composition (4)

4 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
Lnx1-Jam4-Numb complex	IGSF5 LNX1 NUMB	P49757 Q8TBB1 Q9NSI5	1:1:1	Compleat	Compleat:HC1827	16832352
NUMB-ITCH	ITCH NUMB	P49757 Q96J02	0:0	SPIKE
NUMB-TP53-MDM2 complex	MDM2 NUMB TP53	P04637 P49757 Q00987	1:1:1	CompleatCORUM	Compleat:HC2860CORUM:3172	18172499
	NUMB	P49757	4	PDB	PDB:5njkPDB:5njj

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Protein Organic Solvent Precipitation	Mass spectrometry [LTQ-FT Ultra]	1	32384937

Sequence, Structure & Domains12

Sequences

Length

651

Mass

70,804

Sequence

MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQMQDAKKAETDKIVVGSSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNAVPEVEGEAESISSLCSQITNAFSTPEDPFSSAPMTKPVTVVAPQSPTFQANGTDSAFHVLAKPAHTALAPVAMPVRETNPWAHAPDAANKEIAATCSGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQASAAPLQPVLQPPPPTAISQPASPFQGNAFLTSQPVPVGVVPALQPAFVPAQSYPVANGMPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNPFSSDLQKTFEIEL

Alternative Products

Event=Alternative splicing; Named isoforms=9; Name=1; Synonyms=p72; IsoId=P49757-1; Sequence=Displayed; Name=2; Synonyms=p66; IsoId=P49757-2; Sequence=VSP_004349; Name=3; Synonyms=p71; IsoId=P49757-3; Sequence=VSP_004348; Name=4; Synonyms=p65; IsoId=P49757-4; Sequence=VSP_004348, VSP_004349; Name=5; IsoId=P49757-5; Sequence=VSP_047756, VSP_004349; Name=6; IsoId=P49757-6; Sequence=VSP_004348, VSP_047756, VSP_004349; Name=7; IsoId=P49757-7; Sequence=VSP_047756, VSP_053763, VSP_004349; Name=8; IsoId=P49757-8; Sequence=VSP_004348, VSP_047756, VSP_053763, VSP_004349; Name=9; IsoId=P49757-9; Sequence=VSP_053745, VSP_004349

Alternative Sequence

1..254; Missing (in isoform 9); 68..78; Missing (in isoform 3, isoform 4, isoform 6 and isoform 8); 219..316; Missing (in isoform 5, isoform 6, isoform 7 and isoform 8); 317..365; Missing (in isoform 7 and isoform 8); 366..413; Missing (in isoform 2, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9)

3D Structural Models

Turn

72..74; 100..102; 134..137

Helix

28..33; 55..71; 77..79; 111..113; 151..168

Beta Strand

38..48; 50..53; 84..90; 92..99; 105..110; 114..119; 126..133; 138..149

3D Structure

X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

235..252; Low complexity; 436..449; Basic and acidic residues; 453..466; Low complexity; 630..644; Polar residues

Domain (FT)

33..193; PID

Region

228..255; Disordered; 419..483; Disordered; 623..651; Disordered

Clinical Relevance5

Disease Involvement

Cancer-related genes

Antibody (2)

HPA002874 CAB011478

Interaction Protein (11)

ENSG00000006125ENSG00000072201ENSG00000085832ENSG00000108953ENSG00000122705ENSG00000135679ENSG00000141510ENSG00000146648ENSG00000164924ENSG00000166913ENSG00000170027

Interaction Count

11

Interaction Dataset (2)

biogrid_opencellintact_biogrid

Supporting Publications13

PMID	Title	Abstract
28590090	Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.	No abstract available
28986585	Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.	No abstract available
29148239	Metabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.	No abstract available
30646616	Preferential Localization of MUC1 Glycoprotein in Exosomes Secreted by Non-Small Cell Lung Carcinoma Cells.	THBS1, ANXA6, HIST1H4A, COL18A1, MDK, SRGN, ENO1, TUBA4A, SLC3A2, GPI, MIF, MUC1, TALDO1, SLC7A5, ICAM1, HSP90AA1, G6PD, and LRP1 were found to be expressed in exosomes at more than 5-fold higher level as compared to total cellular membrane proteins.
30950185	Unique Protein Profiles of Extracellular Vesicles as Diagnostic Biomarkers for Early and Advanced Non-Small Cell Lung Cancer.	No abstract available
32089743	Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism.	No abstract available
33709510	Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.	No abstract available
34817906	Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform.	No abstract available
36398564	Differential ultracentrifugation enables deep plasma proteomics through enrichment of extracellular vesicles.	No abstract available
37786918	Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples.	No abstract available

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Entry name NUMB	UniProt ID P49757	EVMP confidence score 0.60
Supporting publications (n) 13	Transmembrane count	Protein classification Cancer-related genesPredicted intracellular proteins