EV MemProt Atlas

Basic Information10

Protein Names

Vasodilator-stimulated phosphoprotein (VASP)

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000125753

Entrez Gene Symbol

VASP

Gene Description

Vasodilator stimulated phosphoprotein

Chromosome

19

Position

45506579-45526989

Supporting publications (n)

9

EVMP confidence score

0.63

Fluorescence & Localization5

Cell SpecificLymphatic endothelial cellsSingle-Nuclei Brain Specificendothelial cellBlood Cell SpecificNK-cellBlood Lineage SpecificNK-cells

Function & Pathway7

Protein Function

Predicted intracellular proteins

Cellular Component (10)

Molecular Function (4)

Biological Process (3)

KEGG (8)

Reactome (8)

Mediation Categories (5)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence99

Enzyme-Mediated Modification (69)

69 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
VASP	PRKAA1	Q13131	T	278	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20191567 SIGNOR:17082196 ProtMapper:17082196
VASP	PRKAA1	Q13131	S	322	phosphorylation	SIGNOR_ProtMapperSIGNORProtMapper	ProtMapper:21945940 SIGNOR:21945940
VASP	PRKAG1	P54619	T	278	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20191567 SIGNOR:17082196 ProtMapper:17082196
VASP	PRKAG1	P54619	S	322	phosphorylation	SIGNOR_ProtMapperSIGNORProtMapper	ProtMapper:21945940 SIGNOR:21945940
VASP	PRKAB2	O43741	T	278	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20191567 SIGNOR:17082196 ProtMapper:17082196
VASP	PRKAG3	Q9UGI9	T	278	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20191567 SIGNOR:17082196 ProtMapper:17082196
VASP	PRKAG3	Q9UGI9	S	322	phosphorylation	SIGNOR_ProtMapperSIGNORProtMapper	ProtMapper:21945940 SIGNOR:21945940
VASP	PRKAB1	Q9Y478	T	278	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20191567 SIGNOR:17082196 ProtMapper:17082196
VASP	PRKAB1	Q9Y478	S	322	phosphorylation	SIGNOR_ProtMapperSIGNORProtMapper	ProtMapper:21945940 SIGNOR:21945940
VASP	PRKAG2	Q9UGJ0	T	278	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:20191567 SIGNOR:17082196 ProtMapper:17082196

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Ligand-Receptor Signaling (16)

16 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
extracellular	extracellular	OmniPath	No	No	Yes	No	No
intracellular	intracellular	LOCATE	No	No	Yes	No	No
intracellular	intracellular	ComPPI	No	No	Yes	No	No
intracellular	intracellular	GO_Intercell	No	No	Yes	No	No
intracellular	intracellular	UniProt_location	No	No	Yes	No	No
intracellular	intracellular	OmniPath	No	No	Yes	No	No
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	Yes	No	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	Yes	No	No
tight_junction	tight_junction	GO_Intercell	Yes	Yes	Yes	No	No
tight_junction	tight_junction	OmniPath	Yes	Yes	Yes	No	No

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Regulatory Interaction Network (11)

11 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KAPCA	P17612	VASP	P50552	Yes	Yes	No	WangphosphoELM_MIMPAdhesomeMIMPPhosphoSite_MIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAKinexus_KEAHPRD_KEASIGNOR_ProtMapperHPRD-phosPhosphoSite_ProtMapper	SIGNOR:16197368 KEA:8182057 ProtMapper:8182057 KEA:7925440 Adhesome:10851246 Adhesome:8836115 HPRD:10851246 ProtMapper:12576312 KEA:9685341 SIGNOR:8182057 HPRD-phos:12576312 SIGNOR:12576312 KEA:10851246 Adhesome:10922374 HPRD-phos:20058876 ProtMapper:20058876 KEA:94237860 ProtMapper:17287340 KEA:12576312 HPRD:8182057 ProtMapper:16197368 KEA:10922374 HPRD-phos:17287340 ProtMapper:20166139 HPRD:10922374 Adhesome:8182057 KEA:14679200 HPRD-phos:20166139
VASP	P50552	KAPCA	P17612	Yes	Yes	No	HPRDSIGNORAdhesome	HPRD:8182057 SIGNOR:12576312 Adhesome:10851246 Adhesome:10922374 SIGNOR:16197368 HPRD:10851246 Adhesome:8836115 HPRD:10922374 Adhesome:8182057 SIGNOR:8182057
AAPK2	P54646	VASP	P50552	Yes	No	Yes	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	ProtMapper:21945940 SIGNOR:21945940 SIGNOR:17082196 ProtMapper:17082196
KS6A1	Q15418	VASP	P50552	Yes	No	Yes	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:24097826 PhosphoSite:18637808 PhosphoSite:20463151 PhosphoSite:21423205 ProtMapper:21423205 PhosphoSite:21451103 PhosphoSite:14679200 PhosphoSite:8182057 SIGNOR:21423205
TRIM9	Q9C026	VASP	P50552	Yes	No	Yes	SIGNORHPRDHuRIIntActSPIKE_LC	IntAct:32296183 IntAct:16189514 HPRD:16189514 SIGNOR:26702829 SPIKE_LC:16189514
KGP1	Q13976	VASP	P50552	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperPhosphoSite_KEAHPRDKinexus_KEAPhosphoSite_ProtMapperNetworKIN_KEABEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperSparser_ProtMapperHPRD-phos	KEA:15694326 KEA:8182057 ProtMapper:8182057 KEA:7925440 ProtMapper:21702043 ProtMapper:15212693 HPRD:10851246 ProtMapper:12576312 KEA:9685341 SIGNOR:14679200 ProtMapper:20483741 HPRD-phos:12576312 SIGNOR:12576312 KEA:10851246 HPRD-phos:20058876 ProtMapper:20058876 KEA:94237860 ProtMapper:14551241 ProtMapper:14679200 ProtMapper:17287340 KEA:12576312 HPRD:8182057 KEA:10922374 HPRD-phos:17287340 ProtMapper:20166139 SIGNOR:15066263 HPRD:10922374 KEA:17570479 KEA:14679200 HPRD-phos:20166139
	COMPLEX:O43741_P54619_P54646_Q13131_Q9UGI9_Q9UGJ0_Q9Y478	VASP	P50552	Yes	No	Yes	SIGNOR	SIGNOR:21945940 SIGNOR:17082196
RAPH1	Q70E73	VASP	P50552	Yes	Yes	No	SIGNORBioGRID	SIGNOR:20417104 BioGRID:15469845
VASP	P50552	PUR9	P31939	Yes	Yes	No	SIGNOR	SIGNOR:18845790
KPCD1	Q15139	VASP	P50552	Yes	Yes	No	Sparser_ProtMapperPhosphoSite_norefiPTMnetProtMapperRLIMS-P_ProtMapperWangPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:16197368 PhosphoSite:25001901 PhosphoSite:7925440 ProtMapper:24401601 PhosphoSite:18195108 PhosphoSite:30814249 PhosphoSite:26784437 PhosphoSite:15265792 PhosphoSite:18559661 PhosphoSite:23611601 PhosphoSite:33393955 PhosphoSite:10922374 PhosphoSite:11748216 PhosphoSite:15178555 PhosphoSite:9685341 PhosphoSite:23846685 PhosphoSite:20958117 PhosphoSite:26336132 PhosphoSite:17684063 ProtMapper:23846685 PhosphoSite:16283430 PhosphoSite:16339778 PhosphoSite:11919161 PhosphoSite:18079117 PhosphoSite:25961942 PhosphoSite:25759389 PhosphoSite:33737052 PhosphoSite:27620165 ProtMapper:28051168

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Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	ACAT2 AMOT CPNE3 VASP	O75131 P50552 Q4VCS5 Q9BWD1	0:0:0:0	Havugimana2012	Havugimana2012:C_277
	VASP	P50552	4	PDB	PDB:8yvl

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Protein Organic Solvent Precipitation	Mass spectrometryMass spectrometry [LTQ-FT Ultra]Western blotting	1	32384937

Sequence, Structure & Domains16

Sequences

Length

380

Mass

39,830

Sequence

MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP

3D Structural Models

Turn

18..20; 42..45

Helix

96..114; 226..231; 341..376

Beta Strand

5..12; 14..16; 25..27; 34..41; 46..55; 60..66; 79..86; 88..95

3D Structure

Electron microscopy (3); NMR spectroscopy (1); X-ray crystallography (5)

Domain & Motif Annotations

Compositional Bias

162..192; Pro residues; 224..233; Low complexity; 312..342; Polar residues

Repeat

344..358; 1; 359..373; 2

Motif

234..237; KLKR

Coiled Coil

337..373

Domain (CC)

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.; DOMAIN: The WH1 domain mediates interaction with XIRP1.

Domain (FT)

2..113; WH1

Region

111..343; Disordered; 225..377; EVH2; 225..245; EVH2 block A; 259..278; EVH2 block B; 343..377; EVH2 block C; 344..373; 2 X 15 AA tandem repeats of L-[EQ]-[KR]-[MV]-K-[EQ]-E-[IL]-[IL]-E-[AEV]-[FV]-[KRV]-[KQ]-E

Protein Families

Ena/VASP family

Sequence Similarities

Belongs to the Ena/VASP family.

Clinical Relevance5

Drugs

HYDROCHLOROTHIAZIDE

Antibody (2)

CAB004612 HPA005724

Interaction Protein (11)

ENSG00000075624ENSG00000100505ENSG00000108518ENSG00000109971ENSG00000138443ENSG00000154380ENSG00000159840ENSG00000168334ENSG00000184009ENSG00000196405ENSG00000213066

Interaction Count

11

Interaction Dataset (3)

biogrid_opencellintact_biogridintact_biogrid_opencell

Supporting Publications9

PMID	Title	Abstract
25776846	Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens.	No abstract available
28211531	Proteomic analysis of urinary extracellular vesicles from high Gleason score prostate cancer.	No abstract available
28240915	Quantitative Proteomic Analysis of Serum Exosomes from Patients with Locally Advanced Pancreatic Cancer Undergoing Chemoradiotherapy.	No abstract available
29441425	Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins.	No abstract available
31414377	Global extracellular vesicle proteomic signature defines U87-MG glioma cell hypoxic status with potential implications for non-invasive diagnostics.	No abstract available
32331267	Mass Spectrometry-Based Proteomic Characterization of Cutaneous Melanoma Ectosomes Reveals the Presence of Cancer-Related Molecules.	No abstract available
33017416	Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers.	No abstract available
33169421	A novel method of high-purity extracellular vesicle enrichment from microliter-scale human serum for proteomic analysis.	No abstract available
34202855	Proteomic Profiling of Ectosomes Derived from Paired Urothelial Bladder Cancer and Normal Cells Reveals the Presence of Biologically-Relevant Molecules.	No abstract available

Entry name VASP	UniProt ID P50552	EVMP confidence score 0.63
Supporting publications (n) 9	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins