EV MemProt Atlas

Basic Information

Protein Names

Ephrin-B2 (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L)

Protein Class

Cancer-related genesPlasma proteinsPredicted membrane proteins

Protein Function

Cancer-related genes:Candidate cancer biomarkers

Transmembrane

230..250; Helical

Transmembrane Count

1

Ensembl

ENSG00000125266

Entrez Gene Symbol

EFNB2

Gene Synonym

EPLG5Htk-LHTKLLERK5MGC126226MGC126227MGC126228

Gene Description

Ephrin B2

Chromosome

13

Position

106489745-106535662

Frequency

1

EVMP Score

0.25

Fluorescence & Localization

Tissue Specificlymphoid tissueCell SpecificCone photoreceptor cellsSingle-Nuclei Brain SpecificleukocyteBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway

Protein Function

Cancer-related genes:Candidate cancer biomarkers

Cellular Component

Molecular Function

Biological Process

KEGG

hsa04360 Axon guidance

Reactome

Mediation Categories

Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EFNB2	EPHB2	P29323	Y	304	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEAphosphoELM	KEA:15096211 phosphoELM:15096211 KEA:15951569
EFNB2	EGFR	P00533	Y	330	phosphorylation	BEL-Large-Corpus_ProtMapperProtMapper	ProtMapper:15951569

Ligand-Receptor Signaling

53 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane_phobius	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_sosui	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	Yes	No

Page 6 of 6Previous

Regulatory Interaction Network

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
EFNB2	P52799	EPHB4	P54760	Yes	Yes	No	iTALKICELLNETHINTCellPhoneDB_CellinkerSignaLink3HPMR_talklrHPMRCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDIntActDLRP_talklrRamilowski2015_Baccin2019WangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBaccin2019CellinkerSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:15114347 Cellinker:9718367 connectomeDB2020:9718367 HPRD:9718367 SignaLink3:12051776 Cellinker:12051776 Baccin2019:9718367 connectomeDB2020:7534404 HINT:26481148 SignaLink3:18988627 HINT:16867992 Baccin2019:7534404 LRdb:11114742 CellChatDB:15114347 SignaLink3:23331499 HPMR:7534404 Baccin2019:12051776 HINT:33961781 CellTalkDB:15114347 SignaLink3:9718367 ICELLNET:24003208 IntAct:26481148 connectomeDB2020:12051776 HPRD:12051776 ICELLNET:15114347 SignaLink3:9576626 Cellinker:7534404
EFNB2	P52799	EPHB2	P29323	Yes	Yes	No	iTALKICELLNETProtMapperHINTCellPhoneDB_CellinkerSignaLink3HPMR_talklrCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDRamilowski2015_Baccin2019DLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBioGRIDBaccin2019CellinkerREACH_ProtMapperSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	CellTalkDB:15114347 BioGRID:11780069 ICELLNET:24003208 connectomeDB2020:11780069 Cellinker:15114347 ProtMapper:28719605 HINT:11780069 LRdb:11114742 CellChatDB:15114347 ICELLNET:15114347 SignaLink3:11780069 SignaLink3:23331499 SignaLink3:9267020 connectomeDB2020:9330863Baccin2019:933086311780069HPRD:11780069 HINT:33961781
EFNB2	P52799	EPHB1	P54762	Yes	Yes	No	iTALKICELLNETCellPhoneDB_CellinkerSignaLink3HPMR_talklrHPMRCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDRamilowski2015_Baccin2019DLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBaccin2019CellinkerSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:9330863 Cellinker:15114347 Baccin2019:9330863 HPRD:10066262 Baccin2019:10066262 connectomeDB2020:8559144 LRdb:11114742 CellChatDB:15114347 SignaLink3:23331499 HPRD:8559144 connectomeDB2020:9330863 Cellinker:10066262 CellTalkDB:15114347 Baccin2019:8559144 HPMR:9330863 ICELLNET:24003208 connectomeDB2020:10066262 ICELLNET:15114347 SignaLink3:9576626 Cellinker:8559144
EFNB2	P52799	EPHB3	P54753	Yes	Yes	No	iTALKICELLNETSIGNORCellPhoneDB_CellinkerSignaLink3HPMR_talklrCellChatDBDLRP_CellinkertalklrRamilowski2015_Baccin2019DLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBBaccin2019CellinkerSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:9330863 CellTalkDB:15114347 connectomeDB2020:9330863 ICELLNET:24003208 SIGNOR:9330863 Cellinker:15114347 SIGNOR:8559144 LRdb:11114742 CellChatDB:15114347 ICELLNET:15114347 SignaLink3:9576626 SignaLink3:23331499 Baccin2019:9330863
EFNB2	P52799	EPHA3	P29320	Yes	Yes	No	Fantom5_LRdbCellTalkDBHPRD_LRdbiTALKtalklrconnectomeDB2020HPRDHINTHPRD_talklrBioGRIDSignaLink3CellinkerLRdbSTRING_talklrRamilowski2015	HINT:9195962 BioGRID:8559144 HINT:8559144 SignaLink3:8559144 connectomeDB2020:8559144 SignaLink3:9195962 LRdb:8559144 SignaLink3:23331499 CellTalkDB:8559144 HPRD:8559144 Cellinker:32589946

Protein Complex Composition

0 records.

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	ELISA	3	40465195 37322475 36982312

Sequence, Structure & Domains

Sequences

Length

333

Mass

36,923

Sequence

MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGCIIFIVIIITLVVLLLKYRRRHRKHSPQHTTTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

3D Structural Models

Turn

44..46; 103..105

Helix

83..87; 142..144; 151..155

Beta Strand

28..34; 47..50; 57..62; 65..68; 75..81; 98..101; 108..113; 119..122; 130..135; 145..149; 159..164

3D Structure

NMR spectroscopy (1); X-ray crystallography (8)

Domain & Motif Annotations

Compositional Bias

175..185; Basic and acidic residues; 190..213; Polar residues

Motif

331..333; PDZ-binding

Domain (FT)

28..164; Ephrin RBD

Region

165..213; Disordered

Protein Families

Ephrin family

Sequence Similarities

Belongs to the ephrin family.

Clinical Relevance

Disease Involvement

Cancer-related genes

Antibody

HPA008999 CAB009368

Interaction Protein

ENSG00000196411

Interaction Count

1

Interaction Dataset

intact_biogrid

Protein symbol EFNB2	UniProt ID P52799	EVMP score 0.25
Frequency 1	Transmembrane count 1	Protein classification Cancer-related genesPlasma proteinsPredicted membrane proteins