Protein detail

EPHB1

Ephrin type-B receptor 1 (EC 2.7.10.1) (ELK) (EPH tyrosine kinase 2) (EPH-like kinase 6) (EK6) (hEK6) (Neuronally-expressed EPH-related tyrosine kinase) (NET) (Tyrosine-protein kinase receptor EPH-2)

Entry name EPHB1	UniProt ID P54762	EVMP confidence score 0.50
Supporting publications (n) 5	Transmembrane count 1	Protein classification EnzymesPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Ephrin type-B receptor 1 (EC 2.7.10.1) (ELK) (EPH tyrosine kinase 2) (EPH-like kinase 6) (EK6) (hEK6) (Neuronally-expressed EPH-related tyrosine kinase) (NET) (Tyrosine-protein kinase receptor EPH-2)

Protein Class (3)

EnzymesPredicted intracellular proteinsPredicted membrane proteins

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:Tyr protein kinases
Predicted intracellular proteins

Transmembrane

541..563; Helical

Transmembrane Count

Ensembl

ENSG00000154928

Entrez Gene Symbol

EPHB1

Gene Synonym (2)

EPHT2Hek6

Gene Description

EPH receptor B1

Chromosome

Position

134795260-135260467

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization7

Tissue SpecificplacentaCell SpecificCytotrophoblastsBlood Cell Specificmemory B-cellBlood Lineage SpecificB-cellsSecretome LocationSecreted to bloodSecretome FunctionCell adhesion

Function & Pathway6

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:Tyr protein kinases
Predicted intracellular proteins

Cellular Component (11)

Page 1 of 2

Molecular Function (5)

KEGG

hsa04360 Axon guidance

Reactome (5)

Mediation Categories (4)

Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence84

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EPHB1	EPHA1	P21709	Y	928	phosphorylation	Li2012

Ligand-Receptor Signaling (68)

68 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
extracellular	extracellular	OmniPath	No	No	No	Yes	No
intracellular	intracellular	LOCATE	No	No	No	Yes	No
intracellular	intracellular	ComPPI	No	No	No	Yes	No
intracellular	intracellular	GO_Intercell	No	No	No	Yes	No
intracellular	intracellular	UniProt_location	No	No	No	Yes	No
intracellular	intracellular	OmniPath	No	No	No	Yes	No
cell_surface_ligand	cell_surface_ligand	Baccin2019	Yes	No	No	Yes	No
cell_surface_ligand	cell_surface_ligand	OmniPath	Yes	No	No	Yes	No
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	Yes	No
cell_adhesion	cell_adhesion	Zhong2015	Yes	Yes	No	Yes	No

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Regulatory Interaction Network (10)

10 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
EPHB1	P54762	PPAC	P24666	Yes	Yes	No	HPRDPhosphoPointSignaLink3	SignaLink3:23331499 SignaLink3:9499402 HPRD:9499402
EFNB1	P98172	EPHB1	P54762	Yes	Yes	No	iTALKICELLNETSIGNORCellPhoneDB_CellinkerSignaLink3HPMR_talklrHPMRCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDRamilowski2015_Baccin2019DLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBaccin2019CellinkerSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:9330863 Baccin2019:10669731 HPRD:12223469 Cellinker:12223469 Cellinker:15114347 Baccin2019:12223469 Baccin2019:9330863 Baccin2019:11713248 Cellinker:11713248 connectomeDB2020:11713248 connectomeDB2020:12223469 SIGNOR:11713248 HPMR:11713248 HPRD:10669731 connectomeDB2020:10669731 LRdb:11114742 CellChatDB:15114347 Cellinker:10669731 SignaLink3:23331499 connectomeDB2020:9330863 ICELLNET:24003208 ICELLNET:15114347 SignaLink3:9267020
EFNB2	P52799	EPHB1	P54762	Yes	Yes	No	iTALKICELLNETCellPhoneDB_CellinkerSignaLink3HPMR_talklrHPMRCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDRamilowski2015_Baccin2019DLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBaccin2019CellinkerSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:9330863 Cellinker:15114347 Baccin2019:9330863 HPRD:10066262 Baccin2019:10066262 connectomeDB2020:8559144 LRdb:11114742 CellChatDB:15114347 SignaLink3:23331499 HPRD:8559144 connectomeDB2020:9330863 Cellinker:10066262 CellTalkDB:15114347 Baccin2019:8559144 HPMR:9330863 ICELLNET:24003208 connectomeDB2020:10066262 ICELLNET:15114347 SignaLink3:9576626 Cellinker:8559144
EPHB1	P54762	GRB7	Q14451	Yes	Yes	No	Li2012Lit-BM-17SignaLink3	Lit-BM-17:12223469 SignaLink3:23331499 SignaLink3:12223469
EPHB1	P54762	NCK1	P16333	Yes	Yes	No	HPRDWangPhosphoPointSignaLink3	SignaLink3:23331499 SignaLink3:9430661 HPRD:9430661
EPHB1	P54762	MP2K4	P45985	Yes	Yes	No	SIGNORACSN	ACSN:18000759 ACSN:15574420 ACSN:14585353 ACSN:19568798 SIGNOR:26319181 ACSN:19151919 ACSN:18855897 ACSN:12040186 ACSN:11274345 ACSN:11777939 ACSN:17496910 ACSN:12738796 ACSN:21614932
EPHB1	P54762	STAT3	P40763	Yes	Yes	No	SIGNOR	SIGNOR:29079839
EPHB1	P54762	CSKI1	Q8WXD9	Yes	Yes	No	Sparser_ProtMapperiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperREACH_ProtMapper	ProtMapper:23181695 SIGNOR:23181695
EPHB1	P54762	NRCAM	Q92823	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	ProtMapper:24023801 SIGNOR:24023801
CNKR1	Q969H4	EPHB1	P54762	Yes	Yes	No	SIGNOR	SIGNOR:26319181

Protein Complex Composition (4)

4 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
EPHB1 EPHB6 PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1 PRKAG2 PRKAG3 ULK1 ULK2	O15197 O43741 O75385 P54619 P54646 P54762 Q13131 Q8IYT8 Q9UGI9 Q9UGJ0 Q9Y478	1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8523
ACO1 EPHB1 EPHB6 MX2 PFKP UBC	O15197 P0CG48 P20592 P21399 P54762 Q01813	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4672
EPHB1 PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1 PRKAG2 PRKAG3 ULK1 ULK2 YWHAE YWHAH	O43741 O75385 P54619 P54646 P54762 P62258 Q04917 Q13131 Q8IYT8 Q9UGI9 Q9UGJ0 Q9Y478	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7768
EPHB1	P54762	9	PDB	PDB:5mjaPDB:5mjbPDB:3zfx

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometry	6	29562735 24505114 30071318 36635400 40098346 31588238

Sequence, Structure & Domains13

Sequences

Length

984

Mass

109,885

Sequence

MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSPTAMA

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P54762-1; Sequence=Displayed; Name=2; IsoId=P54762-5; Sequence=VSP_056017; Name=3; IsoId=P54762-6; Sequence=VSP_056018, VSP_056019

Alternative Sequence

1..439; Missing (in isoform 2); 158..242; VNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNG -> LALQGHSRPARKLKAAPTAPPTAAPLQRRLPSAPVGPVITERTLTLQKWHALASHQVPAMLSPSSMRRPSFWSGTLQGRQVGGMM (in isoform 3); 243..984; Missing (in isoform 3)

3D Structural Models

Turn

711..714; 824..827; 922..924

Helix

616..618; 659..673; 705..710; 718..737; 747..749; 788..790; 793..798; 803..818; 830..838; 851..860; 865..867; 871..883; 885..887; 916..921; 926..928; 929..935; 940..943; 948..954; 959..975

Beta Strand

441..445; 447..453; 464..473; 482..495; 501..512; 514..517; 521..524; 619..627; 632..638; 646..652; 683..687; 689..692; 694..698; 750..752; 758..760; 783..786; 889..891

3D Structure

NMR spectroscopy (2); X-ray crystallography (6)

Domain & Motif Annotations

Motif

982..984; PDZ-binding

Domain (FT)

19..201; Eph LBD; 322..432; Fibronectin type-III 1; 433..528; Fibronectin type-III 2; 619..882; Protein kinase; 911..975; SAM

Protein Families (3)

Protein kinase superfamily
Tyr protein kinase family
Ephrin receptor subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Clinical Relevance5

Drugs (60)

RITLECITINIB DEURUXOLITINIB XL019 JAK3 INHIBITOR IV ZOTIRACICLIB COMPOUND 5E [PMID: 28580438]ILGINATINIB FILGOTINIB PF-06263276 SOLCITINIB -SAR 20347.00 IVARMACITINIB ABROCITINIB JAK INHIBITOR 20A JAK INHIBITOR 17B POVORCITINIB FEDRATINIB IZENCITINIB MOMELOTINIB PF-956980 SJ988497 PEFICITINIB LASW1393 JAK3 INHIBITOR 34 CEE321 PACRITINIB DECERNOTINIB COMPOUND 8L [PMID: 36053746]UPADACITINIB VANDETANIB DEUCRAVACITINIB COMPOUND 13AC [PMID: 33256400]NEZULCITINIB ROPSACITINIB HESPERADIN PROGESTERONE COMPOUND 18E [PMID: 31670517]JAK3 INHIBITOR 32 COMPOUND 19A [PMID: 24359159]GDC-0214 BMS-911543 OCLACITINIB AZD1480 COMPOUND 30 [PMID: 37057760]COMPOUND 25AP [PMID: 37796543]BARICITINIB BREPOCITINIB LONDAMOCITINIB RECOMBINANT FIBROBLAST GROWTH FACTOR 2 RUXOLITINIB DELGOCITINIB GOLIDOCITINIB CERDULATINIB LEPZACITINIB JAK3 INHIBITOR II ITACITINIB LORPUCITINIB WXFL10203614 TOFACITINIB NIK INHIBITOR 12F

Antibody

HPA067740

Interaction Protein

ENSG00000141738

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications5

PMID	Title	Abstract
34980157	Proteomic analysis of extracellular vesicles secreted by primary human epithelial endometrial cells reveals key proteins related to embryo implantation.	No abstract available
37886648	Pathological mechanisms of type 1 diabetes in children: investigation of the exosomal protein expression profile.	No abstract available
38343172	Analysis of secreted small extracellular vesicles from activated human microglial cell lines reveals distinct pro- and anti-inflammatory proteomic profiles.	No abstract available
39025337	Shotgun proteomics of thyroid carcinoma exosomes - Insight into the role of exosomal proteins in carcinogenesis and thyroid homeostasis.	No abstract available
39277144	The proteomic signature of circulating extracellular vesicles following intracerebral hemorrhage: Novel insights into mechanisms underlying recovery.	No abstract available