Protein detail
EPHA4
Ephrin type-A receptor 4 (EC 2.7.10.1) (EPH-like kinase 8) (EK8) (hEK8) (Tyrosine-protein kinase TYRO1) (Tyrosine-protein kinase receptor SEK)
Entry name EPHA4 | UniProt ID | EVMP confidence score 0.88 |
Supporting publications (n) 24 | Transmembrane count 1 | Protein classification Cancer-related genesEnzymesPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins |
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.70High >= 0.60Medium >= 0.40Low >= 0.30Basic Information
Protein Names
Ephrin type-A receptor 4 (EC 2.7.10.1) (EPH-like kinase 8) (EK8) (hEK8) (Tyrosine-protein kinase TYRO1) (Tyrosine-protein kinase receptor SEK)
Protein Class (5)
Cancer-related genesEnzymesPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins
Protein Function (5)
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- Enzymes
- Cancer-related genes:Candidate cancer biomarkers
- Kinases:Tyr protein kinases
Transmembrane
548..569; Helical
Transmembrane Count
1
Ensembl
Entrez Gene Symbol
Gene Synonym (2)
Hek8TYRO1
Gene Description
EPH receptor A4
Chromosome
2
Position
221418027-221574202
Supporting publications (n)
24
EVMP confidence score
0.88
Fluorescence & Localization
Cell SpecificNeutrophilsBlood Cell Specificneutrophil
Function & Pathway
Protein Function (5)
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- Enzymes
- Cancer-related genes:Candidate cancer biomarkers
- Kinases:Tyr protein kinases
Cellular Component (19)
- GO:0005737 cytoplasm
- GO:0005741 mitochondrial outer membrane
- GO:0005886 plasma membrane
- GO:0005912 adherens junction
- GO:0009986 cell surface
- GO:0030175 filopodium
- GO:0030424 axon
- GO:0030425 dendrite
- GO:0031594 neuromuscular junction
- GO:0031901 early endosome membrane
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Molecular Function (13)
- GO:0001540 amyloid-beta binding
- GO:0004672 protein kinase activity
- GO:0004713 protein tyrosine kinase activity
- GO:0005004 GPI-linked ephrin receptor activity
- GO:0005005 transmembrane-ephrin receptor activity
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0016301 kinase activity
- GO:0042731 PH domain binding
- GO:0042802 identical protein binding
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Biological Process (3)
Reactome (7)
Canonical Pathways (2)
- M247 Pid insulin glucose pathway
- M7955 Sig insulin receptor pathway in cardiac myocytes
Mediation Categories (4)
Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationReceptor-signaling mediation
Relations & Evidence88
Enzyme-Mediated Modification (1)
1 record.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| EPHA4 | PTPRO | Q16827 | Y | 602 | phosphorylation | KEA | KEA:11136979 |
Ligand-Receptor Signaling (70)
70 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| extracellular | extracellular | HPMR | Yes | ||||
| extracellular | extracellular | OmniPath | Yes | ||||
| intracellular | intracellular | LOCATE | Yes | ||||
| intracellular | intracellular | ComPPI | Yes | ||||
| intracellular | intracellular | GO_Intercell | Yes | ||||
| intracellular | intracellular | UniProt_location | Yes | ||||
| intracellular | intracellular | OmniPath | Yes | ||||
| cell_surface_ligand | cell_surface_ligand | connectomeDB2020 | Yes | Yes | |||
| cell_surface_ligand | cell_surface_ligand | Baccin2019 | Yes | Yes | |||
| cell_surface_ligand | cell_surface_ligand | OmniPath | Yes | Yes |
Regulatory Interaction Network (12)
12 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| EFNB3 | Q15768 | EPHA4 | P54764 | Yes | Yes | iTALKICELLNETSIGNORHINTCellPhoneDB_CellinkerDIPHPMR_talklrCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDIntActDLRP_talklrRamilowski2015_Baccin2019WangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBioGRIDBaccin2019CellinkerSTRING_talklrEMBRACECellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb | HINT:9484836connectomeDB2020:12383247Cellinker:12383247Cellinker:9484836Cellinker:15114347IntAct:23812375BioGRID:9484836Baccin2019:12649481Baccin2019:12383247DIP:23812375connectomeDB2020:9484836CellChatDB:15114347HPRD:9484836Cellinker:12649481Baccin2019:9484836LRdb:12094214HINT:33961781HPRD:12383247HINT:23812375ICELLNET:24003208SIGNOR:9330863ICELLNET:15114347 | |
| EFNA3 | P52797 | EPHA4 | P54764 | Yes | Yes | iTALKICELLNETSIGNORCellPhoneDB_CellinkerHPMR_talklrCellChatDBHPRD_LRdbDLRP_CellinkertalklrHPRDRamilowski2015_Baccin2019DLRP_talklrWangRamilowski2015HPMR_LRdbCellPhoneDBHPRD_talklrBaccin2019CellinkerSTRING_talklrEMBRACECellCallCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb | HPRD:12496762ICELLNET:24003208connectomeDB2020:12496762SIGNOR:9330863Cellinker:15114347LRdb:11114742ICELLNET:15114347CellChatDB:15114347Baccin2019:933086312496762connectomeDB2020:9330863 |
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Protein Complex Composition (4)
4 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| EIF3AEIF3BEIF3FEIF3HEIF3JEIF4A1EIF4A2EIF4EEIF4G1EIF4G2EIF4G3EPHA4NCBP1PABPC1UBC | O00303O15372O43432O75822P06730P0CG48P11940P54764P55884P60842P78344Q04637Q09161Q14152Q14240 | 1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC8104 | ||
| EFNA2EPHA4 | O43921P54764 | 1:1 | PDB | PDB:2wo3 | ||
| EPHA4 | P54764 | 2 | PDB | PDB:5jr2PDB:2wo1PDB:4w4zPDB:4bk4PDB:3ckhPDB:4w50 | ||
| EFNB3EPHA4 | P54764Q15768 | 2:2 | PDB | PDB:4bkf |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| PRotein Organic Solvent Precipitation；Differential Ultracentrifugation | Mass spectrometry | 1 | 32384937 |
Sequence, Structure & Domains
Sequences
Length
986
Mass
109,860
Sequence
MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTVLLVSVSGSVVLVVILIAAFVISRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P54764-1; Sequence=Displayed; Name=2; IsoId=P54764-2; Sequence=VSP_056016
Alternative Sequence
1..53; MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGG -> MK (in isoform 2)
3D Structural Models
Turn
40..42; 373..375; 538..540
Helix
36..38; 108..110; 139..141; 426..428
Beta Strand
29..35; 46..53; 55..60; 62..65; 66..72; 77..79; 82..85; 93..96; 97..105; 114..116; 119..130; 131..133; 142..149; 154..159; 162..173; 178..190; 192..203; 207..209; 212..214; 226..230; 237..248; 257..262; 266..269; 272..275; 304..306; 333..340; 343..349; 361..368; 385..388; 390..393; 395..401; 405..416; 418..423; 429..435; 447..452; 457..461; 473..483; 489..500; 508..517; 528..531
3D Structure
NMR spectroscopy (1); X-ray crystallography (16)
Domain & Motif Annotations
Motif
984..986; PDZ-binding
Domain (CC)
The protein kinase domain mediates interaction with NGEF.
Domain (FT)
30..209; Eph LBD; 328..439; Fibronectin type-III 1; 440..537; Fibronectin type-III 2; 621..882; Protein kinase; 911..975; SAM
Protein Families (3)
- Protein kinase superfamily
- Tyr protein kinase family
- Ephrin receptor subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Clinical Relevance
Disease Involvement
Cancer-related genes
Related Diseases
Biomarker
Terminated
Drugs
Antibody
Interaction Protein (4)
ENSG00000108947ENSG00000133216ENSG00000184349ENSG00000243364
Interaction Count
4
Interaction Dataset
intact_biogrid
Supporting Publications24
| PMID | Title | Related sentences |
|---|---|---|
| 40311616 | Integrative proteomic profiling of tumor and plasma extracellular vesicles identifies a diagnostic biomarker panel for colorectal cancer. | No related sentences available |
| 40619995 | A 96-Well Ultrafiltration Approach for the High-Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium. | No related sentences available |
| 40784529 | Serum-derived exosome proteomics unveils the distinct and adjustable nature of the dampness constitution in traditional Chinese medicine. | No related sentences available |
| 41216884 | Extracellular Vesicles From Multiple Sclerosis White Matter Exhibit Synaptic, Mitochondrial, Complement and Ageing-Related Pathway Dysregulation. | No related sentences available |
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