Protein detail

BCAR1

Breast cancer anti-estrogen resistance protein 1 (CRK-associated substrate) (Cas scaffolding protein family member 1) (p130cas)

Protein symbol BCAR1	UniProt ID P56945	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Breast cancer anti-estrogen resistance protein 1 (CRK-associated substrate) (Cas scaffolding protein family member 1) (p130cas)

Protein Class

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000050820

Entrez Gene Symbol

BCAR1

Gene Synonym

CASCASS1CrkasP130Cas

Gene Description

BCAR1 scaffold protein, Cas family member

Chromosome

Position

75228181-75268053

Frequency

EVMP Score

0.38

Fluorescence & Localization

Tissue Specificadipose tissueCell SpecificMicrogliaSingle-Nuclei Brain Specificcentral nervous system macrophageSecretome LocationSecreted to extracellular matrixSecretome FunctionDevelopmental protein

Function & Pathway

Protein Function

Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

KEGG

Reactome

Canonical Pathways

M77 Pid wnt signaling pathway
M266 Pid ncadherin pathway
M5493 Wnt signaling

Mediation Categories

Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

77 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
BCAR1	FGFR1	P11362	Y	456	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
BCAR1	FGFR1	P11362	Y	128	phosphorylation	PhosphoNetworksSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapper	ProtMapper:11019781 SIGNOR:11019781 phosphoELM:12601080 SIGNOR:12601080 ProtMapper:12601080 KEA:12601080
BCAR1	FGFR1	P11362	Y	327	phosphorylation	PhosphoNetworksSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapper	ProtMapper:12601080 SIGNOR:12601080 phosphoELM:12601080 KEA:12601080
BCAR1	FGFR1	P11362	Y	306	phosphorylation	PhosphoNetworksSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapper	ProtMapper:12601080 SIGNOR:12601080 phosphoELM:12601080 KEA:12601080
BCAR1	FGFR1	P11362	Y	249	phosphorylation	PhosphoNetworksSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapper	ProtMapper:12601080 SIGNOR:12601080 phosphoELM:12601080 KEA:12601080
BCAR1	FGFR1	P11362	Y	410	phosphorylation	PhosphoNetworksSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapper	ProtMapper:12601080 SIGNOR:12601080 phosphoELM:12601080 KEA:12601080
BCAR1	FGFR1	P11362	Y	165	phosphorylation	PhosphoNetworks
BCAR1	FGFR1	P11362	Y	224	phosphorylation	PhosphoNetworks
BCAR1	FGFR1	P11362	Y	267	phosphorylation	PhosphoNetworks
BCAR1	FGFR1	P11362	Y	287	phosphorylation	PhosphoNetworks

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Ligand-Receptor Signaling

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
adhesion_cytoskeleton_adaptor	intracellular_intercellular_related	Adhesome	Yes	No	No	No	No
intracellular_intercellular_related	intracellular_intercellular_related	OmniPath	Yes	No	No	No	No

Regulatory Interaction Network

13 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
FAK1	Q05397	BCAR1	P56945	Yes	Yes	No	SPIKEHPRD_MIMPSIGNORProtMapperHINTPhosphoSite_KEAphosphoELM_KEADOMINOMPPIHPRDCui2007CA1WangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperAdhesomeSPIKE_LCHPRD-phos	Adhesome:10753828 HINT:11514617 HINT:12119061 HPRD:12119061 MPPI:10545505 HINT:8649427 ProtMapper:11604500 ProtMapper:12119061 HPRD:7479864 SPIKE_LC:10092764 HPRD:9360968 HPRD:11514617 DOMINO:9285683 HINT:9148935 SPIKE_LC:9285683 SPIKE_LC:9148935 KEA:8621540 SIGNOR:11604500 ProtMapper:9360983 SPIKE:9285683 Adhesome:7479864 Adhesome:9285683 Adhesome:8662921 KEA:9360968 HINT:9360983 Adhesome:12135674 Adhesome:8810278 HPRD-phos:12119061 KEA:12119061 KEA:12480533 ProtMapper:24939893 Adhesome:10592173 HINT:9285683 ProtMapper:9360968 Adhesome:11514617 ProtMapper:19138410 SPIKE:10092764 HPRD-phos:9360983 KEA:11604500 KEA:9360983 Adhesome:21855630 Adhesome:11119718 Adhesome:15673687 HPRD:9360983 Adhesome:11577104 Adhesome:11839772 Adhesome:8649427 Adhesome:12615911 HINT:10545505 HPRD-phos:9360968 Adhesome:9038154 KEA:8670206 HINT:21034468 HINT:9360968 CA1:8662921 SPIKE:9148935 KEA:12972425 Adhesome:18233957 HINT:7479864
SRC	P12931	BCAR1	P56945	Yes	Yes	No	SPIKEHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperHINTPhosphoSite_KEAphosphoELM_KEALi2012Lit-BM-17HPRDCui2007CA1IntActWangNetworKIN_KEAPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetELMKEABioGRIDHPRD_KEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapperSparser_ProtMapperAdhesomeSPIKE_LCHPRD-phos	Adhesome:8070403 Adhesome:10542110 ELM:10542110 ProtMapper:21937722 phosphoELM:11604500 CA1:8070403 ProtMapper:20927395 KEA:12972425 ELM:9360983 ProtMapper:11604500 SIGNOR:22710723 Lit-BM-17:10085298 HINT:10487518 Lit-BM-17:15020686 ProtMapper:15212693 ProtMapper:12972425 Adhesome:10487518 HINT:9148935 KEA:15817476 BioGRID:12397603 Lit-BM-17:12397603 ProtMapper:17016520 ProtMapper:21765460 SIGNOR:12972425 IntAct:17038317 ProtMapper:23974298 Adhesome:17038317 ProtMapper:21301050 SIGNOR:11604500 ProtMapper:22710723 SPIKE:15272013 KEA:15316024 HINT:10542110 ProtMapper:17389395 IntAct:10542110 BioGRID:17129785 KEA:12480533 SPIKE_LC:15272013 Lit-BM-17:17038317 HINT:10739664 ProtMapper:21963850 ProtMapper:24962474 ProtMapper:30422386 Lit-BM-17:10487518 Adhesome:11514617 Lit-BM-17:11514617 ProtMapper:19138410 ProtMapper:23457577 IntAct:21034468 KEA:11604500 HPRD-phos:19901323 Adhesome:10085298 HINT:17038317 HPRD-phos:12972425 Adhesome:12972425 HPRD:12972425 Adhesome:12615911 HINT:12397603 HPRD-phos:17389395 Lit-BM-17:10739664 Lit-BM-17:17129785 HINT:17129785 ProtMapper:19901323 KEA:8670206 ProtMapper:25976166 HINT:10085298 HINT:21034468 Lit-BM-17:10542110 ProtMapper:20103658 KEA:17570479 Adhesome:10739664 Adhesome:12397603 HPRD-phos:17016520 ProtMapper:28808245
ABL1	P00519	BCAR1	P56945	Yes	Yes	No	WangAdhesomeSIGNORPhosphoPointHPRDdbPTMBioGRIDSPIKE_LCSPIKE	Adhesome:8810278 Adhesome:17129785 Adhesome:7780740 dbPTM:16212419 SPIKE_LC:7780740 dbPTM:15951569 SPIKE_LC:20841568 HPRD:7780740 SIGNOR:17615370 dbPTM:19534553 dbPTM:18083107 BioGRID:7780740 SPIKE:7780740 SPIKE:20841568
FYN	P06241	BCAR1	P56945	Yes	Yes	Yes	DOMINOWangAdhesomeNetPathPhosphoPointSIGNORELMHINTHPRDHuRIIntActSPIKE_LCLit-BM-17SPIKE	ELM:10542110 NetPath:9020138 Lit-BM-17:17474147 Adhesome:17474147 IntAct:31413325 Lit-BM-17:9360983 ELM:9360983 SIGNOR:24586768 SPIKE:17474147 SPIKE_LC:9020138 HINT:9188452 Adhesome:17129785 SPIKE:9020138 HINT:9360983 HINT:10739664 Adhesome:9188452 SPIKE_LC:17474147 SPIKE:16713569 Lit-BM-17:10739664 Adhesome:9360983 IntAct:9360983 HPRD:9188452 Lit-BM-17:9188452 SPIKE_LC:16713569 IntAct:17474147 Adhesome:10739664 Adhesome:11181827 DOMINO:17474147
PTPRF	P10586	BCAR1	P56945	Yes	No	Yes	HPRDWangSIGNORAdhesome	SIGNOR:10320483 Adhesome:10822386 HPRD:10320483 Adhesome:10320483 HPRD:15588985 HPRD:10822386
BCAR1	P56945	SMAD3	P84022	Yes	No	Yes	NetPathSIGNOR	NetPath:18321991 SIGNOR:18321991
FGFR1	P11362	BCAR1	P56945	Yes	Yes	No	WangPhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMLi2012SIGNOR_ProtMapper	ProtMapper:11019781 SIGNOR:11019781 phosphoELM:12601080 SIGNOR:12601080 ProtMapper:12601080 KEA:12601080
PTN12	Q05209	BCAR1	P56945	Yes	No	Yes	DOMINOWangAdhesomeSIGNORDEPODHPRDHINTBioGRIDIntActSPIKE_LC	BioGRID:12714323 SIGNOR:11432829 HPRD:12714323 DEPOD:9748319 DOMINO:9285683 SPIKE_LC:9285683 HPRD:9285683 HINT:9748319 HPRD:9920935 Adhesome:9285683 Adhesome:11432829 DEPOD:8887669 Adhesome:8887669 HINT:9285683 IntAct:9285683 HINT:8887669 DEPOD:9920935 DEPOD:9285683 HPRD:8887669 HPRD:9748319 DEPOD:12714323
PTK6	Q13882	BCAR1	P56945	Yes	Yes	No	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:33391524 SIGNOR:22084245 ProtMapper:25938342 PhosphoSite:15870699 ProtMapper:22084245
BCAR1	P56945	PKN3	Q6P5Z2	Yes	Yes	No	SIGNOR	SIGNOR:30422386

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Protein Complex Composition

9 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
FAK\u2013p130Cas\u2013MT1 complex	BCAR1 MMP14 PTK2	P50281 P56945 Q05397	0:0:0	CORUM	CORUM:6921	22291036
HT_SC_Cluster41	BCAR1 PHB1 PHB2	P35232 P56945 Q99623	1:1:1	Compleat	Compleat:HC3492
NPHP1-NPHP4-BCAR1 complex	BCAR1 NPHP1 NPHP4	O15259 O75161 P56945	0:0:0	CORUM	CORUM:6381	15661758
	ARCN1 ARF4 ATP9A BCAR1 PDE6D RHBDD3 SCPEP1 SFT2D2 SLC66A2 TM9SF4 UBC ZDHHC13	O43924 O75110 O95562 P0CG48 P18085 P48444 P56945 Q8IUH4 Q8N2U9 Q92544 Q9HB40 Q9Y3P4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4577
	ARCN1 ARF4 BCAR1 GOLT1B PDE6D RHBDD3 SCPEP1 SFT2D3 SLC66A2 TM9SF4 UBC ZDHHC13	O43924 P0CG48 P18085 P48444 P56945 Q587I9 Q8IUH4 Q8N2U9 Q92544 Q9HB40 Q9Y3E0 Q9Y3P4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3877
	ARCN1 ARF4 BCAR1 PDE6D RGP1 RHBDD3 SCPEP1 SFT2D1 SLC66A2 TM9SF4 UBC ZDHHC13	O43924 P0CG48 P18085 P48444 P56945 Q8IUH4 Q8N2U9 Q8WV19 Q92544 Q92546 Q9HB40 Q9Y3P4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6145
	ARCN1 BCAR1 GOLT1B PDE6D RHBDD3 SCPEP1 SFT2D1 SLC66A2 TM9SF4 TRIM23 UBC ZDHHC12	O43924 P0CG48 P36406 P48444 P56945 Q8N2U9 Q8WV19 Q92544 Q96GR4 Q9HB40 Q9Y3E0 Q9Y3P4	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6282
	BCAR1	P56945	2	PDB	PDB:1wyx
	BCAR1 SH2D3C	P56945 Q8N5H7	2:2	PDB	PDB:3t6g

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationMicrofluidics-Based Methods	Mass spectrometry	1	40545963

Sequence, Structure & Domains

Sequences

Length

870

Mass

93,372

Sequence

MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA

Alternative Products

Event=Alternative splicing; Named isoforms=8; Name=1; IsoId=P56945-1; Sequence=Displayed; Name=2; IsoId=P56945-2; Sequence=VSP_043559; Name=3; IsoId=P56945-3; Sequence=VSP_045355; Name=4; IsoId=P56945-4; Sequence=VSP_046127, VSP_046128; Name=5; IsoId=P56945-5; Sequence=VSP_046748; Name=6; IsoId=P56945-6; Sequence=VSP_046749; Name=7; IsoId=P56945-7; Sequence=VSP_046750; Name=8; IsoId=P56945-8; Sequence=VSP_046751

Alternative Sequence

1..4; MNHL -> MLTHRPQEAEQRGRTPGPSFEW (in isoform 2); 1..4; MNHL -> MQGK (in isoform 4); 1..4; MNHL -> ME (in isoform 5); 1..4; MNHL -> MPAKPFLSSVLLSWKVLDFSGPGPQGTGQPCSCGHWAEGQGGPPEPAGGP (in isoform 6); 1..4; MNHL -> MHCPGEAPLAAPRPTPKDPCLR (in isoform 7); 1..4; MNHL -> MSVP (in isoform 8); 64..211; Missing (in isoform 4); 304; A -> AVSKCQGNARARLRLWGVW (in isoform 3)

3D Structural Models

Helix

37..39; 57..59; 740..770; 775..801; 806..835; 840..870

Beta Strand

5..12; 29..34; 43..48; 51..56; 60..68

3D Structure

NMR spectroscopy (3); X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

73..85; Pro residues; 97..111; Polar residues; 135..151; Polar residues; 416..426; Basic and acidic residues; 427..444; Low complexity; 626..655; Polar residues

Motif

635..643; SH3-binding

Domain (CC)

Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9 (By similarity).; DOMAIN: A serine-rich region promotes activation of the serum response element (SRE).; DOMAIN: The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

Domain (FT)

3..65; SH3

Region

70..156; Disordered; 115..416; Substrate for kinases; 411..449; Disordered; 609..658; Disordered; 715..734; Disordered; 746..796; Divergent helix-loop-helix motif

Protein Families

CAS family

Sequence Similarities

Belongs to the CAS family.

Clinical Relevance

Disease Involvement

Diabetes mellitus

Drugs

CELECOXIB

Antibody

CAB000443 HPA042282 HPA063796

Interaction Protein

ENSG00000010810ENSG00000061938ENSG00000095370ENSG00000108953ENSG00000125731ENSG00000126603ENSG00000127947ENSG00000128245ENSG00000136205ENSG00000137936ENSG00000164924ENSG00000166913ENSG00000167193ENSG00000169398ENSG00000170027ENSG00000196396ENSG00000197122ENSG00000241685ENSG00000277363

Interaction Count

Interaction Dataset

intact_biogridbiogrid_opencellbiogrid_bioplexbiogrid_opencell_bioplex