Protein detail

RAB8A

Ras-related protein Rab-8A (EC 3.6.5.2) (Oncogene c-mel)

Entry name RAB8A	UniProt ID P61006	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information6

Protein Names

Ras-related protein Rab-8A (EC 3.6.5.2) (Oncogene c-mel)

Protein Function (3)

Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Ensembl

ENSG00000167461

Entrez Gene Symbol

RAB8A

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization6

Tissue SpecificovaryCell SpecificDecidual stromal cellsSingle-Nuclei Brain SpecificfibroblastSecretome LocationIntracellular and membraneSecretome FunctionReceptor

Function & Pathway7

Protein Function (3)

Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Cellular Component (24)

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Molecular Function (7)

Biological Process (3)

KEGG (7)

Reactome (17)

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Mediation Categories (4)

Adhesion and uptake mediationFusion and delivery mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence30

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
RAB8A	STK24	Q9Y6E0	T	72	phosphorylation	PhosphoSiteSIGNOR	SIGNOR:32227113
RAB8A	MAP3K7	O43318	T	72	phosphorylation	PhosphoSiteSIGNOR	SIGNOR:32227113
RAB8A	LRRK2	Q5S007	T	72	phosphorylation	Sparser_ProtMapperSIGNORProtMapperREACH_ProtMapperPhosphoSite	ProtMapper:29482628 ProtMapper:30209220 SIGNOR:32227113 ProtMapper:29357897 ProtMapper:29308544
RAB8A	PINK1	Q9BXM7	S	111	phosphorylation	Sparser_ProtMapperSIGNORProtMapperRLIMS-P_ProtMapperREACH_ProtMapper	ProtMapper:28860483 SIGNOR:31361120 ProtMapper:26471730

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (7)

7 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
CE290	O15078	RAB8A	P61006	Yes	Yes	No	SIGNOR	SIGNOR:18694559
	COMPLEX:A8MTZ0_Q3SYG4_Q8IWZ6_Q8N3I7_Q8NFJ9_Q8TAM2_Q96RK4_Q9BXC9	RAB8A	P61006	Yes	Yes	No	SIGNOR	SIGNOR:17574030
PINK1	Q9BXM7	RAB8A	P61006	Yes	No	Yes	Sparser_ProtMapperSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperREACH_ProtMapper	ProtMapper:32227113 ProtMapper:28860483 ProtMapper:31361120 ProtMapper:27070490 SIGNOR:31361120 ProtMapper:26471730
M3K7	O43318	RAB8A	P61006	Yes	Yes	No	SIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:34914695 ProtMapper:32227113 PhosphoSite:30709905 PhosphoSite:32601174 PhosphoSite:32227113 PhosphoSite:34088839 SIGNOR:32227113 PhosphoSite:33887226 PhosphoSite:31292254 PhosphoSite:32017888
STK24	Q9Y6E0	RAB8A	P61006	Yes	Yes	No	SIGNOR_ProtMapperSIGNORPhosphoSite_ProtMapperProtMapper	ProtMapper:32227113 SIGNOR:32227113
LRRK2	Q5S007	RAB8A	P61006	Yes	Yes	No	Sparser_ProtMapperSIGNORProtMapperHINTIntActSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper	ProtMapper:32227113 HINT:26824392 ProtMapper:29482628 ProtMapper:31054512 ProtMapper:33546409 ProtMapper:30209220 IntAct:26824392 SIGNOR:32227113 ProtMapper:29357897 IntAct:32017888 ProtMapper:29308544 ProtMapper:33887226 HINT:32017888
RPGR	Q92834	RAB8A	P61006	Yes	Yes	No	SIGNOR	SIGNOR:20631154

Protein Complex Composition (11)

11 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HD-RAB8A-OPTN complex	HTT OPTN RAB8A	P42858 P61006 Q96CV9	1:1:1	CompleatCORUM	CORUM:2241Compleat:HC1303	11137014
HOOK2-PCM1-RAB8A complex	HOOK2 PCM1 RAB8A	P61006 Q15154 Q96ED9	0:0:0	CORUM	CORUM:7442	21998199
HT_DM_Cluster147	AGTRAP RAB10 RAB8A RAB8B SSR1 TSPO TSPO2	B1AH88 P43307 P61006 P61026 Q5TGU0 Q6RW13 Q92930	1:1:1:1:1:1:1	Compleat	Compleat:HC476	22036573
	ASPDH RAB8A	A6ND91 P61006	0:0	hu.MAP
	RAB8A	P61006	5	PDB	PDB:4lhwPDB:6stfPDB:4lhvPDB:6stgPDB:6whe
	OCRL RAB8A	P61006 Q01968	4:4	PDB	PDB:3qbt
	RAB3IL1 RAB8A	P61006 Q8TBN0	2:4	PDB	PDB:4li0
	RAB8A RAB8B	P61006 Q92930	0:0	hu.MAP2
	RAB8A RILPL2	P61006 Q969X0	2:2	PDB	PDB:6sq2PDB:6rirPDB:7lwb
	OPTN RAB8A	P61006 Q96CV9	2:2	PDB	PDB:9ikq

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	6	37786918 34817906 28986585 27894104 39948040 38321535

Sequence, Structure & Domains13

Sequences

Length

207

Mass

23,668

Sequence

MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P61006-1; Sequence=Displayed; Name=2; IsoId=P61006-2; Sequence=VSP_056399

Alternative Sequence

161..205; AFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCV -> RYQSKNGQKIGRQQPPGEQPGSQNHTGPAEEEQLFPMCSSVRNTA (in isoform 2)

3D Structural Models

Turn

38..41; 69..72; 122..124; 152..155

Helix

21..30; 73..78; 93..97; 99..109; 126..128; 133..142; 158..174

Beta Strand

7..14; 34..36; 42..52; 55..64; 65..67; 82..89; 115..121; 146..149

3D Structure

X-ray crystallography (20)

Domain & Motif Annotations

Motif

31..45; Switch 1; 63..80; Switch 2

Domain (CC)

Switch 1, switch 2 and the interswitch regions are characteristic of Rab GTPases and mediate the interactions with Rab downstream effectors. The switch regions undergo conformational changes upon nucleotide binding which drives interaction with specific sets of effector proteins, with most effectors only binding to GTP-bound Rab.

Protein Families (2)

Small GTPase superfamily
Rab family

Sequence Similarities

Belongs to the small GTPase superfamily. Rab family.

Clinical Relevance3

Interaction Protein (6)

ENSG00000072274ENSG00000123240ENSG00000127328ENSG00000138190ENSG00000166128ENSG00000203879

Interaction Count

Interaction Dataset (2)

intact_biogridbiogrid_bioplex

Supporting Publications1

PMID	Title	Abstract
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available