Protein detail

PRAME

Melanoma antigen preferentially expressed in tumors (Opa-interacting protein 4) (OIP-4) (Preferentially expressed antigen of melanoma)

Entry name PRAME	UniProt ID P78395	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Melanoma antigen preferentially expressed in tumors (Opa-interacting protein 4) (OIP-4) (Preferentially expressed antigen of melanoma)

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000185686

Entrez Gene Symbol

PRAME

Gene Synonym (2)

CT130MAPE

Gene Description

PRAME nuclear receptor transcriptional regulator

Chromosome

Position

22547701-22559361

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization5

Cell SpecificEpicardial cellsSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell SpecificbasophilBlood Lineage Specificdendritic cells

Function & Pathway5

Protein Function

Predicted intracellular proteins

Cellular Component (7)

Molecular Function (3)

Biological Process (3)

Mediation Categories

Clinical-translation mediation

Relations & Evidence16

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (9)

9 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
PRAME complex	CUL2 ELOB ELOC LAGE3 OSGEP PRAME RBX1	P62877 P78395 Q13617 Q14657 Q15369 Q15370 Q9NPF4	0:0:0:0:0:0:0	CORUM	CORUM:7556	22912744
PRAME-Cullin2 ubiquitin ligase complex	CUL2 PRAME	P78395 Q13617	0:0	CORUM	CORUM:6736	21822215
PRAME-Elongin C-Elongin B E3 ubiquitin ligase complex	CUL2 ELOB ELOC PRAME RBX1	P62877 P78395 Q13617 Q15369 Q15370	1:1:1:1:1	ComplexPortal	intact:EBI-9010045	27222660 21822215 22649776 23460923 14755292
PRAME-ElonginBC ubiquitin ligase	ELOB ELOC PRAME	P78395 Q15369 Q15370	0:0:0	CORUM	CORUM:6738	21822215
	PRAMEF10 PRAMEF17	O60809 Q5VTA0	0:0	hu.MAP2
	PRAMEF15 PRAMEF4 PRAMEF5	O60810 P0DUQ1 Q5TYX0	0:0:0	hu.MAPhu.MAP2
	PRAMEF4 PRAMEF5 PRAMEF9	O60810 P0DUQ2 Q5TYX0	0:0:0	hu.MAP2
	PRAMEF15 PRAMEF5	P0DUQ1 Q5TYX0	0:0	hu.MAP2
	PRAMEF5 PRAMEF9	P0DUQ2 Q5TYX0	0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	36550367

Sequence, Structure & Domains7

Sequences

Length

509

Mass

57,890

Sequence

MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN

Domain & Motif Annotations

Repeat

116..145; LRR 1; degenerate; 207..231; LRR 2; degenerate; 232..258; LRR 3; degenerate; 259..294; LRR 4; degenerate; 295..320; LRR 5; 321..352; LRR 6; 353..371; LRR 7; 377..404; LRR 8; 405..429; LRR 9

Region

416..509; Mediates interaction with RARA

Protein Families

PRAME family

Sequence Similarities

Belongs to the PRAME family.

Clinical Relevance6

Related Diseases (2)

Malignant haematopoietic neoplasm Solid tumour/cancer

Biomarker

Phase 1; Phase 1/2

Antibody (3)

HPA045153 HPA068562 CAB080138

Interaction Protein (3)

ENSG00000092094ENSG00000154582ENSG00000196976

Interaction Count

Interaction Dataset (2)

biogrid_bioplexintact_biogrid

Supporting Publications1

PMID	Title	Abstract
34167524	Exosomes from cervical cancer cells facilitate pro-angiogenic endothelial reconditioning through transfer of Hedgehog-GLI signaling components.	Changes in angiogenesis-related transcripts of vascular endothelial growth factor VEGF-A, VEGF-B, VEGFR2 and angiopoietin-1, angiopoietin-2, osteopontin were measured in exosome-treated HUVEC and in the exosomal RNA by RT-PCR. Overall, the data showed cervical cancer exosomes promote pro-angiogenic response in endothelial cells via upregulation of Hh-GLI signaling and modulate downstream angiogenesis-related target genes.