Protein detail

X3CL1

Fractalkine (C-X3-C motif chemokine 1) (CX3C membrane-anchored chemokine) (Neurotactin) (Small-inducible cytokine D1) [Cleaved into: Processed fractalkine]

Entry name X3CL1	UniProt ID P78423	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count 1	Protein classification Plasma proteinsPredicted membrane proteinsPredicted secreted proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Fractalkine (C-X3-C motif chemokine 1) (CX3C membrane-anchored chemokine) (Neurotactin) (Small-inducible cytokine D1) [Cleaved into: Processed fractalkine]

Protein Class (3)

Plasma proteinsPredicted membrane proteinsPredicted secreted proteins

Protein Function

Predicted secreted proteins

Transmembrane

342..362; Helical

Transmembrane Count

Ensembl

ENSG00000006210

Entrez Gene Symbol

CX3CL1

Gene Synonym (6)

ABCD-3C3XkineCXC3CXC3CNTNSCYD1

Gene Description

C-X3-C motif chemokine ligand 1

Chromosome

Position

57372477-57385044

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Tissue SpecificliverCell SpecificHepatocytesSecretome LocationSecreted to bloodSecretome FunctionTransport

Function & Pathway7

Protein Function

Predicted secreted proteins

Cellular Component (9)

Molecular Function (8)

Biological Process (3)

KEGG (6)

Reactome (6)

Mediation Categories (5)

Adhesion and uptake mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence62

Ligand-Receptor Signaling (59)

59 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
cell_surface_ligand	cell_surface_ligand	OmniPath	Yes	No	Yes	Yes	No
transmembrane	transmembrane	UniProt_location	No	No	Yes	Yes	No
transmembrane	transmembrane	UniProt_topology	No	No	Yes	Yes	No
transmembrane	transmembrane	UniProt_keyword	No	No	Yes	Yes	No
transmembrane_predicted	transmembrane	OmniPath	No	No	Yes	Yes	No
transmembrane	transmembrane	CellPhoneDB	No	No	Yes	Yes	No
transmembrane	transmembrane	LOCATE	No	No	Yes	Yes	No
transmembrane	transmembrane	Ramilowski_location	No	No	Yes	Yes	No
transmembrane	transmembrane	OmniPath	No	No	Yes	Yes	No
secreted	secreted	Matrisome	No	No	Yes	Yes	No

Page 2 of 6Previous Next

Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
X3CL1	P78423	CX3C1	P49238	Yes	Yes	No	iTALKKirouac2010KEGG-MEDICUSGuide2Pharma_LRdbICELLNETSIGNORHINTCellPhoneDB_CellinkerInnateDBDIPLit-BM-17HPMR_talklrHPMRCellChatDBHPRD_LRdbDLRP_CellinkertalklrscConnectHPRDDLRP_talklrGuide2Pharma_CellinkerWangRamilowski2015HPMR_LRdbCellPhoneDBGuide2Pharma_talklrHPRD_talklrCellinkerSTRING_talklrCellCallGuide2PharmaCellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020SPIKE_LCLRdb	Cellinker:11432858 Cellinker:9390561 LRdb:10699158 SPIKE_LC:16189514 connectomeDB2020:9931005 scConnect:14607932 connectomeDB2020:9390561 SIGNOR:11432858 HINT:9931005 Cellinker:14607932 HPRD:9390561 HPRD:9931005 HPMR:11432858 InnateDB:9931005 HINT:23125415 Guide2Pharma:14607932 connectomeDB2020:11432858 ICELLNET:22633458 Cellinker:9931005 InnateDB:9390561 Lit-BM-17:10488147 DIP:10488147 Lit-BM-17:9931005 connectomeDB2020:14607932 Cellinker:28389721

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	CX3CL1	P78423	4	PDB	PDB:1f2l

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Polymer Precipitation	Western blotting	1	38731868

Sequence, Structure & Domains11

Sequences

Length

397

Mass

42,203

Sequence

MAPISLSWLLRLATFCHLTVLLAGQHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQAVGLLAFLGLLFCLGVAMFTYQSLQGCPRKMAGEMAEGLRYIPRSCGSNSYVLVPV

3D Structural Models

Turn

68..70

Helix

27..29; 45..47; 56..58; 80..90; 93..96

Beta Strand

34..36; 48..53; 59..61; 63..67; 72..75

3D Structure

Electron microscopy (4); NMR spectroscopy (1); X-ray crystallography (5)

Domain & Motif Annotations

Compositional Bias

133..147; Polar residues; 193..202; Polar residues; 219..243; Low complexity

Region

25..100; Chemokine and involved in interaction with ITGAV:ITGB3 and ITGA4:ITGB1; 101..341; Mucin-like stalk; 128..265; Disordered; 289..309; Disordered

Protein Families

Intercrine delta family

Sequence Similarities

Belongs to the intercrine delta family.

Clinical Relevance2

Drugs (3)

FREUND'S ADJUVANT ATEZOLIZUMAB QUETMOLIMAB

Antibody (3)

CAB026192 HPA040361 CAB080311

Supporting Publications1

PMID	Title	Abstract
38439112	Docosahexaenoic acid (DHA) impairs hypoxia-induced cellular and exosomal overexpression of immune-checkpoints and immunomodulatory molecules in different subtypes of breast cancer cells.	MDA-MB-231 and BT-474 cells were treated with 100 µM DHA under hypoxic and normoxic conditions for 24 h. Exosomes were isolated by ultracentrifuge and confirmed by electron microscope and anti-CD9, -CD63, -CD81 immunoblotting. Total RNA from cells and exosomes were extracted and expression of CD39, CD73, CD47, CD80, PD-L1, B7-H3, B7-H4 genes and their related miRs were evaluated by quantitative Real-time PCR.